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Protein

Putative type II secretion system protein M

Gene

gspM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins.Curated

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG12173-MONOMER.
ECOL316407:JW5704-MONOMER.
MetaCyc:EG12173-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative type II secretion system protein M
Short name:
T2SS protein M
Alternative name(s):
Putative general secretion pathway protein M
Transport protein PshM
Gene namesi
Name:gspM
Synonyms:hopZ, pshM
Ordered Locus Names:b3334, JW5704
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12173. gspM.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence analysisAdd
BLAST
Transmembranei17 – 3721HelicalSequence analysisAdd
BLAST
Topological domaini38 – 153116PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 153153Putative type II secretion system protein MPRO_0000207323Add
BLAST

Proteomic databases

PaxDbiP36678.
PRIDEiP36678.

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262464. 145 interactions.
STRINGi511145.b3334.

Structurei

3D structure databases

ProteinModelPortaliP36678.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GSP M family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ZA7S. LUCA.
HOGENOMiHOG000125397.
KOiK02462.
OMAiKCHERCN.
OrthoDBiEOG6MD94K.

Family and domain databases

InterProiIPR007690. T2SS_M.
IPR023229. T2SS_M_periplasmic.
[Graphical view]
PfamiPF04612. T2SSM. 1 hit.
[Graphical view]
SUPFAMiSSF103054. SSF103054. 1 hit.

Sequencei

Sequence statusi: Complete.

P36678-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKSWWAEKS TSEKQIVAAL AVLSLGVFCW LGVIKPIDTY IAEHQSHAQK
60 70 80 90 100
IKKDIKWMQD QASTHGLLGH PALTQPIKNI LLEEAKRENL AITLENGPDN
110 120 130 140 150
TLTIHPVTAP LENVSRWLTT AQVTYGIVIE DLQFTLAGNE EITLRHLSFR

EQQ
Length:153
Mass (Da):17,234
Last modified:December 15, 1998 - v2
Checksum:i8BF3991FB160383C
GO

Sequence cautioni

The sequence AAA58131.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAC36927.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28106 Genomic DNA. Translation: AAC36927.1. Different initiation.
U18997 Genomic DNA. Translation: AAA58131.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76359.2.
AP009048 Genomic DNA. Translation: BAE77957.1.
PIRiA65127.
RefSeqiNP_417793.2. NC_000913.3.
WP_001295161.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76359; AAC76359; b3334.
BAE77957; BAE77957; BAE77957.
GeneIDi947841.
KEGGiecj:JW5704.
eco:b3334.
PATRICi32122100. VBIEscCol129921_3427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28106 Genomic DNA. Translation: AAC36927.1. Different initiation.
U18997 Genomic DNA. Translation: AAA58131.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76359.2.
AP009048 Genomic DNA. Translation: BAE77957.1.
PIRiA65127.
RefSeqiNP_417793.2. NC_000913.3.
WP_001295161.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP36678.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262464. 145 interactions.
STRINGi511145.b3334.

Proteomic databases

PaxDbiP36678.
PRIDEiP36678.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76359; AAC76359; b3334.
BAE77957; BAE77957; BAE77957.
GeneIDi947841.
KEGGiecj:JW5704.
eco:b3334.
PATRICi32122100. VBIEscCol129921_3427.

Organism-specific databases

EchoBASEiEB2091.
EcoGeneiEG12173. gspM.

Phylogenomic databases

eggNOGiENOG410ZA7S. LUCA.
HOGENOMiHOG000125397.
KOiK02462.
OMAiKCHERCN.
OrthoDBiEOG6MD94K.

Enzyme and pathway databases

BioCyciEcoCyc:EG12173-MONOMER.
ECOL316407:JW5704-MONOMER.
MetaCyc:EG12173-MONOMER.

Miscellaneous databases

PROiP36678.

Family and domain databases

InterProiIPR007690. T2SS_M.
IPR023229. T2SS_M_periplasmic.
[Graphical view]
PfamiPF04612. T2SSM. 1 hit.
[Graphical view]
SUPFAMiSSF103054. SSF103054. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Escherichia coli contains a set of genes homologous to those involved in protein secretion, DNA uptake and the assembly of type-4 fimbriae in other bacteria."
    Whitchurch C.B., Mattick J.S.
    Gene 150:9-15(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins."
    Francetic O., Pugsley A.P.
    J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  5. "Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."
    Francetic O., Belin D., Badaut C., Pugsley A.P.
    EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION, TRANSCRIPTIONAL REGULATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiGSPM_ECOLI
AccessioniPrimary (citable) accession number: P36678
Secondary accession number(s): Q2M6Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 15, 1998
Last modified: January 20, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.