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P36659

- CBPA_ECOLI

UniProt

P36659 - CBPA_ECOLI

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Protein
Curved DNA-binding protein
Gene
cbpA, b1000, JW0985
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

DNA-binding protein that preferentially recognizes a curved DNA sequence. It is probably a functional analog of DnaJ; displays overlapping activities with DnaJ, but functions under different conditions, probably acting as a molecular chaperone in an adaptive response to environmental stresses other than heat shock. Lacks autonomous chaperone activity; binds native substrates and targets them for recognition by DnaK. Its activity is inhibited by the binding of CbpM.2 Publications

GO - Molecular functioni

  1. bent DNA binding Source: EcoliWiki
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. protein folding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12193-MONOMER.
ECOL316407:JW0985-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Curved DNA-binding protein
Gene namesi
Name:cbpA
Ordered Locus Names:b1000, JW0985
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12193. cbpA.

Subcellular locationi

Cytoplasmnucleoid 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. nucleoid Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Curved DNA-binding proteinUniRule annotation
PRO_0000169988Add
BLAST

Proteomic databases

PaxDbiP36659.
PRIDEiP36659.

Expressioni

Inductioni

In late stationary phase, by phosphate-starvation conditions.2 Publications

Gene expression databases

GenevestigatoriP36659.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaKP0A6Y84EBI-546131,EBI-542092

Protein-protein interaction databases

DIPiDIP-9249N.
IntActiP36659. 49 interactions.
MINTiMINT-1219908.
STRINGi511145.b1000.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 105
Helixi18 – 3215
Turni34 – 363
Beta strandi39 – 413
Helixi42 – 5615
Helixi59 – 7012

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KQXNMR-A2-72[»]
3UCSX-ray1.87C/D2-73[»]
ProteinModelPortaliP36659.
SMRiP36659. Positions 2-73, 114-303.

Miscellaneous databases

EvolutionaryTraceiP36659.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 6965J
Add
BLAST

Sequence similaritiesi

Contains 1 J domain.

Phylogenomic databases

eggNOGiCOG2214.
HOGENOMiHOG000226716.
KOiK05516.
OMAiTEQTRSI.
OrthoDBiEOG6BPDKP.
PhylomeDBiP36659.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01154. CbpA.
InterProiIPR023859. DNA-bd_curved-DNA.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36659-1 [UniParc]FASTAAdd to Basket

« Hide

MELKDYYAIM GVKPTDDLKT IKTAYRRLAR KYHPDVSKEP DAEARFKEVA    50
EAWEVLSDEQ RRAEYDQMWQ HRNDPQFNRQ FHHGDGQSFN AEDFDDIFSS 100
IFGQHARQSR QRPATRGHDI EIEVAVFLEE TLTEHKRTIS YNLPVYNAFG 150
MIEQEIPKTL NVKIPAGVGN GQRIRLKGQG TPGENGGPNG DLWLVIHIAP 200
HPLFDIVGQD LEIVVPVSPW EAALGAKVTV PTLKESILLT IPPGSQAGQR 250
LRVKGKGLVS KKQTGDLYAV LKIVMPPKPD ENTAALWQQL ADAQSSFDPR 300
KDWGKA 306
Length:306
Mass (Da):34,455
Last modified:November 1, 1997 - v2
Checksum:iA3893D2BFDE75803
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti294 – 30613QSSFD…DWGKA → PVVF in BAA03950. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16500 Genomic DNA. Translation: BAA03950.1.
U00096 Genomic DNA. Translation: AAC74085.1.
AP009048 Genomic DNA. Translation: BAA36142.1.
PIRiF64841.
RefSeqiNP_415520.1. NC_000913.3.
YP_489273.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74085; AAC74085; b1000.
BAA36142; BAA36142; BAA36142.
GeneIDi12932265.
947572.
KEGGiecj:Y75_p0973.
eco:b1000.
PATRICi32117225. VBIEscCol129921_1036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16500 Genomic DNA. Translation: BAA03950.1 .
U00096 Genomic DNA. Translation: AAC74085.1 .
AP009048 Genomic DNA. Translation: BAA36142.1 .
PIRi F64841.
RefSeqi NP_415520.1. NC_000913.3.
YP_489273.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KQX NMR - A 2-72 [» ]
3UCS X-ray 1.87 C/D 2-73 [» ]
ProteinModelPortali P36659.
SMRi P36659. Positions 2-73, 114-303.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-9249N.
IntActi P36659. 49 interactions.
MINTi MINT-1219908.
STRINGi 511145.b1000.

Proteomic databases

PaxDbi P36659.
PRIDEi P36659.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74085 ; AAC74085 ; b1000 .
BAA36142 ; BAA36142 ; BAA36142 .
GeneIDi 12932265.
947572.
KEGGi ecj:Y75_p0973.
eco:b1000.
PATRICi 32117225. VBIEscCol129921_1036.

Organism-specific databases

EchoBASEi EB2110.
EcoGenei EG12193. cbpA.

Phylogenomic databases

eggNOGi COG2214.
HOGENOMi HOG000226716.
KOi K05516.
OMAi TEQTRSI.
OrthoDBi EOG6BPDKP.
PhylomeDBi P36659.

Enzyme and pathway databases

BioCyci EcoCyc:EG12193-MONOMER.
ECOL316407:JW0985-MONOMER.

Miscellaneous databases

EvolutionaryTracei P36659.
PROi P36659.

Gene expression databases

Genevestigatori P36659.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
HAMAPi MF_01154. CbpA.
InterProi IPR023859. DNA-bd_curved-DNA.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An analogue of the DnaJ molecular chaperone in Escherichia coli."
    Ueguchi C., Kakeda M., Yamada H., Mizuno T.
    Proc. Natl. Acad. Sci. U.S.A. 91:1054-1058(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19, SUBCELLULAR LOCATION.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "An analogue of the DnaJ molecular chaperone whose expression is controlled by sigma S during the stationary phase and phosphate starvation in Escherichia coli."
    Yamashino T., Kakeda M., Ueguchi C., Mizuno T.
    Mol. Microbiol. 13:475-483(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "A study of the double mutation of dnaJ and cbpA, whose gene products function as molecular chaperones in Escherichia coli."
    Ueguchi C., Shiozawa T., Kakeda M., Yamada H., Mizuno T.
    J. Bacteriol. 177:3894-3896(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION.
  7. "The cbpA chaperone gene function compensates for dnaJ in lambda plasmid replication during amino acid starvation of Escherichia coli."
    Wegrzyn A., Taylor K., Wegrzyn G.
    J. Bacteriol. 178:5847-5849(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION.
  8. "Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity."
    Azam T.A., Ishihama A.
    J. Biol. Chem. 274:33105-33113(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: BINDING AFFINITY FOR DNA.
  9. "Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid."
    Azam T.A., Hiraga S., Ishihama A.
    Genes Cells 5:613-626(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "CbpA, a DnaJ homolog, is a DnaK co-chaperone, and its activity is modulated by CbpM."
    Chae C., Sharma S., Hoskins J.R., Wickner S.
    J. Biol. Chem. 279:33147-33153(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / DH5-alpha.
  11. "In vivo modulation of a DnaJ homolog, CbpA, by CbpM."
    Chenoweth M.R., Trun N., Wickner S.
    J. Bacteriol. 189:3635-3638(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION BY CBPM.

Entry informationi

Entry nameiCBPA_ECOLI
AccessioniPrimary (citable) accession number: P36659
Secondary accession number(s): P77250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

It binds to curved DNA in a sequence-nonspecific fashion.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi