Curved DNA-binding protein - Escherichia coli (strain K12)

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P36659 (CBPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Curved DNA-binding protein

Gene names

Name:	cbpA
Ordered Locus Names:	b1000, JW0985

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-binding protein that preferentially recognizes a curved DNA sequence. It is probably a functional analog of DnaJ; displays overlapping activities with DnaJ, but functions under different conditions, probably acting as a molecular chaperone in an adaptive response to environmental stresses other than heat shock. Lacks autonomous chaperone activity; binds native substrates and targets them for recognition by DnaK. Its activity is inhibited by the binding of CbpM. Ref.6 Ref.7
Subcellular location	Cytoplasm › nucleoid Ref.1 Ref.9.
Induction	In late stationary phase, by phosphate-starvation conditions. Ref.5 Ref.11
Miscellaneous	It binds to curved DNA in a sequence-nonspecific fashion. HAMAP-Rule MF_01154
Sequence similarities	Contains 1 J domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: HAMAP nucleoid Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	bent DNA binding Inferred from direct assay Ref.8. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 306

306

Curved DNA-binding protein HAMAP-Rule MF_01154

PRO_0000169988

Regions

Domain

5 – 69

Experimental info

Sequence conflict

294 – 306

QSSFD…DWGKA → PVVF in BAA03950. Ref.1

Secondary structure

306

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P36659 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: A3893D2BFDE75803
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FASTA

306

34,455

        10         20         30         40         50         60 
MELKDYYAIM GVKPTDDLKT IKTAYRRLAR KYHPDVSKEP DAEARFKEVA EAWEVLSDEQ 

        70         80         90        100        110        120 
RRAEYDQMWQ HRNDPQFNRQ FHHGDGQSFN AEDFDDIFSS IFGQHARQSR QRPATRGHDI 

       130        140        150        160        170        180 
EIEVAVFLEE TLTEHKRTIS YNLPVYNAFG MIEQEIPKTL NVKIPAGVGN GQRIRLKGQG 

       190        200        210        220        230        240 
TPGENGGPNG DLWLVIHIAP HPLFDIVGQD LEIVVPVSPW EAALGAKVTV PTLKESILLT 

       250        260        270        280        290        300 
IPPGSQAGQR LRVKGKGLVS KKQTGDLYAV LKIVMPPKPD ENTAALWQQL ADAQSSFDPR 


KDWGKA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An analogue of the DnaJ molecular chaperone in Escherichia coli." Ueguchi C., Kakeda M., Yamada H., Mizuno T. Proc. Natl. Acad. Sci. U.S.A. 91:1054-1058(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19, SUBCELLULAR LOCATION. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"An analogue of the DnaJ molecular chaperone whose expression is controlled by sigma S during the stationary phase and phosphate starvation in Escherichia coli." Yamashino T., Kakeda M., Ueguchi C., Mizuno T. Mol. Microbiol. 13:475-483(1994) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"A study of the double mutation of dnaJ and cbpA, whose gene products function as molecular chaperones in Escherichia coli." Ueguchi C., Shiozawa T., Kakeda M., Yamada H., Mizuno T. J. Bacteriol. 177:3894-3896(1995) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE FUNCTION.
[7]	"The cbpA chaperone gene function compensates for dnaJ in lambda plasmid replication during amino acid starvation of Escherichia coli." Wegrzyn A., Taylor K., Wegrzyn G. J. Bacteriol. 178:5847-5849(1996) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE FUNCTION.
[8]	"Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity." Azam T.A., Ishihama A. J. Biol. Chem. 274:33105-33113(1999) [PubMed] [Europe PMC] [Abstract] Cited for: BINDING AFFINITY FOR DNA.
[9]	"Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid." Azam T.A., Hiraga S., Ishihama A. Genes Cells 5:613-626(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"CbpA, a DnaJ homolog, is a DnaK co-chaperone, and its activity is modulated by CbpM." Chae C., Sharma S., Hoskins J.R., Wickner S. J. Biol. Chem. 279:33147-33153(2004) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / DH5-alpha.
[11]	"In vivo modulation of a DnaJ homolog, CbpA, by CbpM." Chenoweth M.R., Trun N., Wickner S. J. Bacteriol. 189:3635-3638(2007) [PubMed] [Europe PMC] [Abstract] Cited for: REGULATION BY CBPM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D16500 Genomic DNA. Translation: BAA03950.1.
U00096 Genomic DNA. Translation: AAC74085.1.
AP009048 Genomic DNA. Translation: BAA36142.1.

PIR

F64841.

RefSeq

NP_415520.1. NC_000913.2.
YP_489273.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KQX	NMR	-	A	2-72	[»]

ProteinModelPortal

P36659.

SMR

P36659. Positions 2-72, 114-303.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9249N.

IntAct

P36659. 49 interactions.

MINT

MINT-1219908.

STRING

511145.b1000.

Proteomic databases

PaxDb

P36659.

PRIDE

P36659.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74085; AAC74085; b1000.
BAA36142; BAA36142; BAA36142.

GeneID

12932265.
947572.

KEGG

ecj:Y75_p0973.
eco:b1000.

PATRIC

32117225. VBIEscCol129921_1036.

Organism-specific databases

EchoBASE

EB2110.

EcoGene

EG12193. cbpA.

Phylogenomic databases

eggNOG

COG2214.

HOGENOM

HOG000226716.

K05516.

OMA

KVANDST.

ProtClustDB

PRK10266.

Enzyme and pathway databases

BioCyc

EcoCyc:EG12193-MONOMER.
ECOL316407:JW0985-MONOMER.

Gene expression databases

Genevestigator

P36659.

Family and domain databases

Gene3D

1.10.287.110. 1 hit.

HAMAP

MF_01154. CbpA.

InterPro

IPR023859. DNA-bd_curved-DNA.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]

Pfam

PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]

PRINTS

PR00625. JDOMAIN.

SMART

SM00271. DnaJ. 1 hit.
[Graphical view]

SUPFAM

SSF46565. DnaJ_N. 1 hit.
SSF49493. HSP40_DnaJ_pep. 2 hits.

PROSITE

PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P36659.

Entry information

Entry name

CBPA_ECOLI

Accession

Primary (citable) accession number: P36659
Secondary accession number(s): P77250

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents