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Protein

Curved DNA-binding protein

Gene

cbpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that preferentially recognizes a curved DNA sequence. It is probably a functional analog of DnaJ; displays overlapping activities with DnaJ, but functions under different conditions, probably acting as a molecular chaperone in an adaptive response to environmental stresses other than heat shock. Lacks autonomous chaperone activity; binds native substrates and targets them for recognition by DnaK. Its activity is inhibited by the binding of CbpM.

Miscellaneous

It binds to curved DNA in a sequence-nonspecific fashion.

GO - Molecular functioni

  • bent DNA binding Source: EcoliWiki
  • unfolded protein binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionChaperone, DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12193-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Curved DNA-binding protein
Gene namesi
Name:cbpA
Ordered Locus Names:b1000, JW0985
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12193. cbpA.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001699881 – 306Curved DNA-binding proteinAdd BLAST306

Proteomic databases

PaxDbiP36659.
PRIDEiP36659.

Expressioni

Inductioni

In late stationary phase, by phosphate-starvation conditions.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaKP0A6Y84EBI-546131,EBI-542092

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261259. 664 interactors.
DIPiDIP-9249N.
IntActiP36659. 49 interactors.
MINTiMINT-1219908.
STRINGi511145.b1000.

Structurei

Secondary structure

1306
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 10Combined sources5
Helixi18 – 32Combined sources15
Turni34 – 36Combined sources3
Beta strandi39 – 41Combined sources3
Helixi42 – 56Combined sources15
Helixi59 – 70Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KQXNMR-A2-72[»]
3UCSX-ray1.87C/D2-73[»]
ProteinModelPortaliP36659.
SMRiP36659.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36659.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 69JAdd BLAST65

Phylogenomic databases

eggNOGiENOG4105DVC. Bacteria.
COG2214. LUCA.
HOGENOMiHOG000226716.
InParanoidiP36659.
KOiK05516.
OMAiFGMVEQE.
PhylomeDBiP36659.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01154. CbpA. 1 hit.
InterProiView protein in InterPro
IPR023859. DNA-bd_curved-DNA.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P36659-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKDYYAIM GVKPTDDLKT IKTAYRRLAR KYHPDVSKEP DAEARFKEVA
60 70 80 90 100
EAWEVLSDEQ RRAEYDQMWQ HRNDPQFNRQ FHHGDGQSFN AEDFDDIFSS
110 120 130 140 150
IFGQHARQSR QRPATRGHDI EIEVAVFLEE TLTEHKRTIS YNLPVYNAFG
160 170 180 190 200
MIEQEIPKTL NVKIPAGVGN GQRIRLKGQG TPGENGGPNG DLWLVIHIAP
210 220 230 240 250
HPLFDIVGQD LEIVVPVSPW EAALGAKVTV PTLKESILLT IPPGSQAGQR
260 270 280 290 300
LRVKGKGLVS KKQTGDLYAV LKIVMPPKPD ENTAALWQQL ADAQSSFDPR

KDWGKA
Length:306
Mass (Da):34,455
Last modified:November 1, 1997 - v2
Checksum:iA3893D2BFDE75803
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti294 – 306QSSFD…DWGKA → PVVF in BAA03950 (PubMed:8302830).CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16500 Genomic DNA. Translation: BAA03950.1.
U00096 Genomic DNA. Translation: AAC74085.1.
AP009048 Genomic DNA. Translation: BAA36142.1.
PIRiF64841.
RefSeqiNP_415520.1. NC_000913.3.
WP_000420621.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74085; AAC74085; b1000.
BAA36142; BAA36142; BAA36142.
GeneIDi947572.
KEGGiecj:JW0985.
eco:b1000.
PATRICi32117225. VBIEscCol129921_1036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16500 Genomic DNA. Translation: BAA03950.1.
U00096 Genomic DNA. Translation: AAC74085.1.
AP009048 Genomic DNA. Translation: BAA36142.1.
PIRiF64841.
RefSeqiNP_415520.1. NC_000913.3.
WP_000420621.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KQXNMR-A2-72[»]
3UCSX-ray1.87C/D2-73[»]
ProteinModelPortaliP36659.
SMRiP36659.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261259. 664 interactors.
DIPiDIP-9249N.
IntActiP36659. 49 interactors.
MINTiMINT-1219908.
STRINGi511145.b1000.

Proteomic databases

PaxDbiP36659.
PRIDEiP36659.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74085; AAC74085; b1000.
BAA36142; BAA36142; BAA36142.
GeneIDi947572.
KEGGiecj:JW0985.
eco:b1000.
PATRICi32117225. VBIEscCol129921_1036.

Organism-specific databases

EchoBASEiEB2110.
EcoGeneiEG12193. cbpA.

Phylogenomic databases

eggNOGiENOG4105DVC. Bacteria.
COG2214. LUCA.
HOGENOMiHOG000226716.
InParanoidiP36659.
KOiK05516.
OMAiFGMVEQE.
PhylomeDBiP36659.

Enzyme and pathway databases

BioCyciEcoCyc:EG12193-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP36659.
PROiPR:P36659.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01154. CbpA. 1 hit.
InterProiView protein in InterPro
IPR023859. DNA-bd_curved-DNA.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCBPA_ECOLI
AccessioniPrimary (citable) accession number: P36659
Secondary accession number(s): P77250
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: May 10, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.