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P36659 (CBPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Curved DNA-binding protein
Gene names
Name:cbpA
Ordered Locus Names:b1000, JW0985
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-binding protein that preferentially recognizes a curved DNA sequence. It is probably a functional analog of DnaJ; displays overlapping activities with DnaJ, but functions under different conditions, probably acting as a molecular chaperone in an adaptive response to environmental stresses other than heat shock. Lacks autonomous chaperone activity; binds native substrates and targets them for recognition by DnaK. Its activity is inhibited by the binding of CbpM. Ref.6 Ref.7

Subcellular location

Cytoplasmnucleoid Ref.1 Ref.9.

Induction

In late stationary phase, by phosphate-starvation conditions. Ref.5 Ref.11

Miscellaneous

It binds to curved DNA in a sequence-nonspecific fashion.

Sequence similarities

Contains 1 J domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandDNA-binding
   Molecular functionChaperone
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleoid

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbent DNA binding

Inferred from direct assay Ref.8. Source: EcoliWiki

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

dnaKP0A6Y84EBI-546131,EBI-542092

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 306306Curved DNA-binding protein HAMAP-Rule MF_01154
PRO_0000169988

Regions

Domain5 – 6965J

Experimental info

Sequence conflict294 – 30613QSSFD…DWGKA → PVVF in BAA03950. Ref.1

Secondary structure

............ 306
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P36659 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: A3893D2BFDE75803

FASTA30634,455
        10         20         30         40         50         60 
MELKDYYAIM GVKPTDDLKT IKTAYRRLAR KYHPDVSKEP DAEARFKEVA EAWEVLSDEQ 

        70         80         90        100        110        120 
RRAEYDQMWQ HRNDPQFNRQ FHHGDGQSFN AEDFDDIFSS IFGQHARQSR QRPATRGHDI 

       130        140        150        160        170        180 
EIEVAVFLEE TLTEHKRTIS YNLPVYNAFG MIEQEIPKTL NVKIPAGVGN GQRIRLKGQG 

       190        200        210        220        230        240 
TPGENGGPNG DLWLVIHIAP HPLFDIVGQD LEIVVPVSPW EAALGAKVTV PTLKESILLT 

       250        260        270        280        290        300 
IPPGSQAGQR LRVKGKGLVS KKQTGDLYAV LKIVMPPKPD ENTAALWQQL ADAQSSFDPR 


KDWGKA 

« Hide

References

« Hide 'large scale' references
[1]"An analogue of the DnaJ molecular chaperone in Escherichia coli."
Ueguchi C., Kakeda M., Yamada H., Mizuno T.
Proc. Natl. Acad. Sci. U.S.A. 91:1054-1058(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19, SUBCELLULAR LOCATION.
Strain: K12.
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"An analogue of the DnaJ molecular chaperone whose expression is controlled by sigma S during the stationary phase and phosphate starvation in Escherichia coli."
Yamashino T., Kakeda M., Ueguchi C., Mizuno T.
Mol. Microbiol. 13:475-483(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"A study of the double mutation of dnaJ and cbpA, whose gene products function as molecular chaperones in Escherichia coli."
Ueguchi C., Shiozawa T., Kakeda M., Yamada H., Mizuno T.
J. Bacteriol. 177:3894-3896(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE FUNCTION.
[7]"The cbpA chaperone gene function compensates for dnaJ in lambda plasmid replication during amino acid starvation of Escherichia coli."
Wegrzyn A., Taylor K., Wegrzyn G.
J. Bacteriol. 178:5847-5849(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE FUNCTION.
[8]"Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity."
Azam T.A., Ishihama A.
J. Biol. Chem. 274:33105-33113(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: BINDING AFFINITY FOR DNA.
[9]"Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid."
Azam T.A., Hiraga S., Ishihama A.
Genes Cells 5:613-626(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"CbpA, a DnaJ homolog, is a DnaK co-chaperone, and its activity is modulated by CbpM."
Chae C., Sharma S., Hoskins J.R., Wickner S.
J. Biol. Chem. 279:33147-33153(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: K12 / DH5-alpha.
[11]"In vivo modulation of a DnaJ homolog, CbpA, by CbpM."
Chenoweth M.R., Trun N., Wickner S.
J. Bacteriol. 189:3635-3638(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REGULATION BY CBPM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16500 Genomic DNA. Translation: BAA03950.1.
U00096 Genomic DNA. Translation: AAC74085.1.
AP009048 Genomic DNA. Translation: BAA36142.1.
PIRF64841.
RefSeqNP_415520.1. NC_000913.3.
YP_489273.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KQXNMR-A2-72[»]
3UCSX-ray1.87C/D2-73[»]
ProteinModelPortalP36659.
SMRP36659. Positions 2-73, 114-303.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9249N.
IntActP36659. 49 interactions.
MINTMINT-1219908.
STRING511145.b1000.

Proteomic databases

PaxDbP36659.
PRIDEP36659.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74085; AAC74085; b1000.
BAA36142; BAA36142; BAA36142.
GeneID12932265.
947572.
KEGGecj:Y75_p0973.
eco:b1000.
PATRIC32117225. VBIEscCol129921_1036.

Organism-specific databases

EchoBASEEB2110.
EcoGeneEG12193. cbpA.

Phylogenomic databases

eggNOGCOG2214.
HOGENOMHOG000226716.
KOK05516.
OMATIRIQPH.
OrthoDBEOG6BPDKP.
PhylomeDBP36659.
ProtClustDBPRK10266.

Enzyme and pathway databases

BioCycEcoCyc:EG12193-MONOMER.
ECOL316407:JW0985-MONOMER.

Gene expression databases

GenevestigatorP36659.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
HAMAPMF_01154. CbpA.
InterProIPR023859. DNA-bd_curved-DNA.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP36659.
PROP36659.

Entry information

Entry nameCBPA_ECOLI
AccessionPrimary (citable) accession number: P36659
Secondary accession number(s): P77250
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene