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Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

pilD

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the pilin precursor during membrane translocation. Required for the assembly of type IV pili and for secretion of most proteins.

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Methyltransferase, Protease, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

MEROPSiA24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzyme
Alternative name(s):
Protein PilD
Protein secretion protein XCPA
Including the following 2 domains:
Leader peptidase (EC:3.4.23.43)
Alternative name(s):
Prepilin peptidase
N-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:pilD
Synonyms:xcpA
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Transmembranei103 – 12321HelicalSequence analysisAdd
BLAST
Transmembranei127 – 14721HelicalSequence analysisAdd
BLAST
Transmembranei158 – 17821HelicalSequence analysisAdd
BLAST
Transmembranei182 – 20221HelicalSequence analysisAdd
BLAST
Transmembranei227 – 24721HelicalSequence analysisAdd
BLAST
Transmembranei254 – 27421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Type 4 prepilin-like proteins leader peptide-processing enzymePRO_0000192626Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

P36642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLWTFLAME PAYFITLATV LGLLVGSFLN VVVYRLPIML ERQWQREAHE
60 70 80 90 100
VLGLPVTEHE RFDLCLPASQ CTQCGHRIRA WENLPVLSYL ALRGRCSACK
110 120 130 140 150
QRISVRYPLV EVGCALLSMV VAWRYGASVE ALVLLPLTWS LLALSLIDHD
160 170 180 190 200
QQLLPDAIVL PGLWLGLIVN YFGVWVPLPD AVCGAVVGYL SLWTVYWLFK
210 220 230 240 250
LVTGKEGMGY GDFKLLALLG AWGGWQVLPL TLLLSSVLGA LVGVYLLRVR
260 270 280
NDSMGTAMPF GPYLAIAGWI AVLWGDEIYA SNMQLLGF
Length:288
Mass (Da):31,972
Last modified:June 1, 1994 - v1
Checksum:iCB7F63F85317F901
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74276 Genomic DNA. Translation: CAA52334.1.
PIRiB36961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74276 Genomic DNA. Translation: CAA52334.1.
PIRiB36961.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA24.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Entry informationi

Entry nameiLEP4_PSEPU
AccessioniPrimary (citable) accession number: P36642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: January 7, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.