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Protein

Magnesium-transporting ATPase, P-type 1

Gene

mgtA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates magnesium influx to the cytosol.

Catalytic activityi

ATP + H2O + Mg2+(Out) = ADP + phosphate + Mg2+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi335 – 3351MagnesiumSequence analysis
Active sitei377 – 37714-aspartylphosphate intermediateBy similarity
Metal bindingi645 – 6451MagnesiumBy similarity
Metal bindingi649 – 6491MagnesiumBy similarity
Metal bindingi713 – 7131MagnesiumSequence analysis
Metal bindingi738 – 7381MagnesiumSequence analysis
Metal bindingi742 – 7421MagnesiumSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-4486-MONOMER.

Protein family/group databases

TCDBi3.A.3.4.1. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium-transporting ATPase, P-type 1 (EC:3.6.3.2)
Alternative name(s):
Mg(2+) transport ATPase, P-type 1
Gene namesi
Name:mgtA
Ordered Locus Names:STM4456
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9898CytoplasmicSequence analysisAdd
BLAST
Transmembranei99 – 11921Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini120 – 1201ExtracellularSequence analysis
Transmembranei121 – 14121Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini142 – 291150CytoplasmicSequence analysisAdd
BLAST
Transmembranei292 – 31221Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini313 – 3219ExtracellularSequence analysis
Transmembranei322 – 33918Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini340 – 699360CytoplasmicSequence analysisAdd
BLAST
Transmembranei700 – 71920Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini720 – 7289ExtracellularSequence analysis
Transmembranei729 – 74820Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini749 – 77022CytoplasmicSequence analysisAdd
BLAST
Transmembranei771 – 79424Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini795 – 8039ExtracellularSequence analysis
Transmembranei804 – 82219Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini823 – 83513CytoplasmicSequence analysisAdd
BLAST
Transmembranei836 – 85520Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini856 – 87015ExtracellularSequence analysisAdd
BLAST
Transmembranei871 – 89020Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini891 – 90212CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 902902Magnesium-transporting ATPase, P-type 1PRO_0000046184Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP36640.
PRIDEiP36640.

Expressioni

Inductioni

Induced by low levels of proline and by osmotic shock. The leader of mgtA mRNA functions as a riboswitch, favoring transcription under low Mg2+ conditions. Under limiting proline levels the MgtL peptide encoded within the mgtA leader cannot be translated, thereby favoring the transcription of the mgtA ORF. Induction by osmotic shock also depends on translational regulation by MgtL (Probable). Induced by low extracellular levels of Mg2+.Curated1 Publication

Interactioni

Protein-protein interaction databases

STRINGi99287.STM4456.

Structurei

3D structure databases

ProteinModelPortaliP36640.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C52. Bacteria.
COG0474. LUCA.
HOGENOMiHOG000265624.
KOiK01531.
OMAiVPEWHAL.
OrthoDBiEOG647TX5.
PhylomeDBiP36640.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006415. P-type_ATPase_IIIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR01836. MGATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01524. ATPase-IIIB_Mg. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36640-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKIITRQLF ARLNRHLPYR LVHRDPLPGA QTAVNATIPP SLSERCLKVA
60 70 80 90 100
AMEQETLWRV FDTHPEGLNA AEVTRAREKH GENRLPAQKP SPWWVHLWVC
110 120 130 140 150
YRNPFNILLT ILGGISYATE DLFAAGVIAL MVGISTLLNF VQEARSTKAA
160 170 180 190 200
DALKAMVSNT ATVLRVINEN GENAWLELPI DQLVPGDIIK LAAGDMIPAD
210 220 230 240 250
LRIIQARDLF VAQASLTGES LPVEKVAATR EPRQNNPLEC DTLCFMGTNV
260 270 280 290 300
VSGTAQAVVM ATGAGTWFGQ LAGRVSEQDN EQNAFQKGIS RVSMLLIRFM
310 320 330 340 350
LVMAPVVLII NGYTKGDWWE AALFALSVAV GLTPEMLPMI VTSTLARGAV
360 370 380 390 400
KLSKQKVIVK HLDAIQNFGA MDILCTDKTG TLTQDKIVLE NHTDISGKPS
410 420 430 440 450
EHVLHCAWLN SHYQTGLKNL LDTAVLEGVD ETAARQLSGR WQKIDEIPFD
460 470 480 490 500
FERRRMSVVV AEDSNVHQLV CKGALQEILN VCTQVRHNGD IVPLDDNMLR
510 520 530 540 550
RVKRVTDTLN RQGLRVVAVA TKYLPAREGD YQRIDESDLI LEGYIAFLDP
560 570 580 590 600
PKETTAPALK ALKASGITVK ILTGDSELVA AKVCHEVGLD AGDVIIGSDI
610 620 630 640 650
EGLSDDALAA LAARTTLFAR LTPMHKERIV TLLKREGHVV GFMGDGINDA
660 670 680 690 700
PALRAADIGI SVDGAVDIAR EAADIILLEK SLMVLEEGVI EGRRTFSNML
710 720 730 740 750
KYIKMTASSN FGNVFSVLVA SAFLPFLPML PLHLLIQNLL YDVSQVAIPF
760 770 780 790 800
DNVDEEQIQK PQRWNPADLG RFMVFFGPIS SIFDILTFCL MWWVFHANTP
810 820 830 840 850
ETQTLFQSGW FVVGLLSQTL IVHMIRTRRL PFIQSRAAWP LMAMTLLVMV
860 870 880 890 900
VGVSLPFSPL ASYLQLQALP LSYFPWLIAI LVGYMTLTQL VKGFYSRRYG

WQ
Length:902
Mass (Da):99,783
Last modified:June 1, 1994 - v1
Checksum:i3D2712E9A074C957
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07843 Genomic DNA. Translation: AAA68988.1.
AE006468 Genomic DNA. Translation: AAL23275.1.
PIRiB57147.
RefSeqiNP_463316.1. NC_003197.1.
WP_001738655.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL23275; AAL23275; STM4456.
GeneIDi1255982.
KEGGistm:STM4456.
PATRICi32387851. VBISalEnt20916_4689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07843 Genomic DNA. Translation: AAA68988.1.
AE006468 Genomic DNA. Translation: AAL23275.1.
PIRiB57147.
RefSeqiNP_463316.1. NC_003197.1.
WP_001738655.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP36640.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM4456.

Protein family/group databases

TCDBi3.A.3.4.1. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbiP36640.
PRIDEiP36640.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL23275; AAL23275; STM4456.
GeneIDi1255982.
KEGGistm:STM4456.
PATRICi32387851. VBISalEnt20916_4689.

Phylogenomic databases

eggNOGiENOG4105C52. Bacteria.
COG0474. LUCA.
HOGENOMiHOG000265624.
KOiK01531.
OMAiVPEWHAL.
OrthoDBiEOG647TX5.
PhylomeDBiP36640.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-4486-MONOMER.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006415. P-type_ATPase_IIIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR01836. MGATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01524. ATPase-IIIB_Mg. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Magnesium transport in Salmonella typhimurium: mgtA encodes a P-type ATPase and is regulated by Mg2+ in a manner similar to that of the mgtB P-type ATPase."
    Tao T., Snavely M.D., Farr S.G., Maguire M.E.
    J. Bacteriol. 177:2654-2662(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiATMA_SALTY
AccessioniPrimary (citable) accession number: P36640
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 17, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.