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Protein

Magnesium-transporting ATPase, P-type 1

Gene

mgtA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates magnesium influx to the cytosol.2 Publications

Catalytic activityi

ATP + H2O + Mg2+(Out) = ADP + phosphate + Mg2+(In).

Kineticsi

  1. KM=12 µM for magnesium ions (at 20 degrees Celsius)1 Publication
  2. KM=29 µM for magnesium ions (at 37 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi335MagnesiumSequence analysis1
    Active sitei3774-aspartylphosphate intermediateBy similarity1
    Metal bindingi645MagnesiumBy similarity1
    Metal bindingi649MagnesiumBy similarity1
    Metal bindingi713MagnesiumSequence analysis1
    Metal bindingi738MagnesiumSequence analysis1
    Metal bindingi742MagnesiumSequence analysis1

    GO - Molecular functioni

    Keywordsi

    Molecular functionHydrolase
    Biological processTransport
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Protein family/group databases

    TCDBi3.A.3.4.1. the p-type atpase (p-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Magnesium-transporting ATPase, P-type 1 (EC:3.6.3.2)
    Alternative name(s):
    Mg(2+) transport ATPase, P-type 1
    Gene namesi
    Name:mgtA
    Ordered Locus Names:STM4456
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 98CytoplasmicSequence analysisAdd BLAST98
    Transmembranei99 – 119Helical; Name=1Sequence analysisAdd BLAST21
    Topological domaini120ExtracellularSequence analysis1
    Transmembranei121 – 141Helical; Name=2Sequence analysisAdd BLAST21
    Topological domaini142 – 291CytoplasmicSequence analysisAdd BLAST150
    Transmembranei292 – 312Helical; Name=3Sequence analysisAdd BLAST21
    Topological domaini313 – 321ExtracellularSequence analysis9
    Transmembranei322 – 339Helical; Name=4Sequence analysisAdd BLAST18
    Topological domaini340 – 699CytoplasmicSequence analysisAdd BLAST360
    Transmembranei700 – 719Helical; Name=5Sequence analysisAdd BLAST20
    Topological domaini720 – 728ExtracellularSequence analysis9
    Transmembranei729 – 748Helical; Name=6Sequence analysisAdd BLAST20
    Topological domaini749 – 770CytoplasmicSequence analysisAdd BLAST22
    Transmembranei771 – 794Helical; Name=7Sequence analysisAdd BLAST24
    Topological domaini795 – 803ExtracellularSequence analysis9
    Transmembranei804 – 822Helical; Name=8Sequence analysisAdd BLAST19
    Topological domaini823 – 835CytoplasmicSequence analysisAdd BLAST13
    Transmembranei836 – 855Helical; Name=9Sequence analysisAdd BLAST20
    Topological domaini856 – 870ExtracellularSequence analysisAdd BLAST15
    Transmembranei871 – 890Helical; Name=10Sequence analysisAdd BLAST20
    Topological domaini891 – 902CytoplasmicSequence analysisAdd BLAST12

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000461841 – 902Magnesium-transporting ATPase, P-type 1Add BLAST902

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP36640.

    Expressioni

    Inductioni

    Induced by low levels of proline and by osmotic shock. The leader of mgtA mRNA functions as a riboswitch, favoring transcription under low Mg2+ conditions. Under limiting proline levels the MgtL peptide encoded within the mgtA leader cannot be translated, thereby favoring the transcription of the mgtA ORF. Induction by osmotic shock also depends on translational regulation by MgtL (Probable). Induced by low extracellular levels of Mg2+.Curated1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi99287.STM4456.

    Structurei

    3D structure databases

    ProteinModelPortaliP36640.
    SMRiP36640.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105C52. Bacteria.
    COG0474. LUCA.
    HOGENOMiHOG000265624.
    KOiK01531.
    OMAiYNNTQQF.
    PhylomeDBiP36640.

    Family and domain databases

    CDDicd02077. P-type_ATPase_Mg. 1 hit.
    Gene3Di3.40.1110.10. 1 hit.
    InterProiView protein in InterPro
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_dom_N.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom_sf.
    IPR008250. ATPase_P-typ_transduc_dom_A_sf.
    IPR036412. HAD-like_sf.
    IPR006415. P-type_ATPase_IIIB.
    IPR001757. P_typ_ATPase.
    PfamiView protein in Pfam
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PRINTSiPR01836. MGATPASE.
    SMARTiView protein in SMART
    SM00831. Cation_ATPase_N. 1 hit.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    SSF81653. SSF81653. 1 hit.
    SSF81660. SSF81660. 1 hit.
    SSF81665. SSF81665. 3 hits.
    TIGRFAMsiTIGR01524. ATPase-IIIB_Mg. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiView protein in PROSITE
    PS00154. ATPASE_E1_E2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P36640-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLKIITRQLF ARLNRHLPYR LVHRDPLPGA QTAVNATIPP SLSERCLKVA
    60 70 80 90 100
    AMEQETLWRV FDTHPEGLNA AEVTRAREKH GENRLPAQKP SPWWVHLWVC
    110 120 130 140 150
    YRNPFNILLT ILGGISYATE DLFAAGVIAL MVGISTLLNF VQEARSTKAA
    160 170 180 190 200
    DALKAMVSNT ATVLRVINEN GENAWLELPI DQLVPGDIIK LAAGDMIPAD
    210 220 230 240 250
    LRIIQARDLF VAQASLTGES LPVEKVAATR EPRQNNPLEC DTLCFMGTNV
    260 270 280 290 300
    VSGTAQAVVM ATGAGTWFGQ LAGRVSEQDN EQNAFQKGIS RVSMLLIRFM
    310 320 330 340 350
    LVMAPVVLII NGYTKGDWWE AALFALSVAV GLTPEMLPMI VTSTLARGAV
    360 370 380 390 400
    KLSKQKVIVK HLDAIQNFGA MDILCTDKTG TLTQDKIVLE NHTDISGKPS
    410 420 430 440 450
    EHVLHCAWLN SHYQTGLKNL LDTAVLEGVD ETAARQLSGR WQKIDEIPFD
    460 470 480 490 500
    FERRRMSVVV AEDSNVHQLV CKGALQEILN VCTQVRHNGD IVPLDDNMLR
    510 520 530 540 550
    RVKRVTDTLN RQGLRVVAVA TKYLPAREGD YQRIDESDLI LEGYIAFLDP
    560 570 580 590 600
    PKETTAPALK ALKASGITVK ILTGDSELVA AKVCHEVGLD AGDVIIGSDI
    610 620 630 640 650
    EGLSDDALAA LAARTTLFAR LTPMHKERIV TLLKREGHVV GFMGDGINDA
    660 670 680 690 700
    PALRAADIGI SVDGAVDIAR EAADIILLEK SLMVLEEGVI EGRRTFSNML
    710 720 730 740 750
    KYIKMTASSN FGNVFSVLVA SAFLPFLPML PLHLLIQNLL YDVSQVAIPF
    760 770 780 790 800
    DNVDEEQIQK PQRWNPADLG RFMVFFGPIS SIFDILTFCL MWWVFHANTP
    810 820 830 840 850
    ETQTLFQSGW FVVGLLSQTL IVHMIRTRRL PFIQSRAAWP LMAMTLLVMV
    860 870 880 890 900
    VGVSLPFSPL ASYLQLQALP LSYFPWLIAI LVGYMTLTQL VKGFYSRRYG

    WQ
    Length:902
    Mass (Da):99,783
    Last modified:June 1, 1994 - v1
    Checksum:i3D2712E9A074C957
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U07843 Genomic DNA. Translation: AAA68988.1.
    AE006468 Genomic DNA. Translation: AAL23275.1.
    PIRiB57147.
    RefSeqiNP_463316.1. NC_003197.2.
    WP_001738655.1. NC_003197.2.

    Genome annotation databases

    EnsemblBacteriaiAAL23275; AAL23275; STM4456.
    GeneIDi1255982.
    KEGGistm:STM4456.
    PATRICifig|99287.12.peg.4689.

    Similar proteinsi

    Entry informationi

    Entry nameiATMA_SALTY
    AccessioniPrimary (citable) accession number: P36640
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: December 20, 2017
    This is version 141 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families