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P36630 (CG22_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G2/mitotic-specific cyclin cig2
Gene names
Name:cig2
Synonyms:cyc17
ORF Names:SPAPB2B4.03
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for the control of the cell cycle at the G2/M and G1/S (mitosis) transition. Interacts with the cdc2 protein kinase to form MPF. Interaction with res2 promotes the phosphorylation of res1 and inhibits MBF-dependent gene transcription. Forms an autoregulating feedback-inhibition loop with MBF which is important for normal regulation of the cell cycle. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis. Negatively regulates conjugation via interacting with cell cycle 'start' genes. Degraded by skp1, pop1 and pop2 in the G2 and M phases of the cell cycle. Ref.1 Ref.3 Ref.4 Ref.6 Ref.9 Ref.10 Ref.11

Subunit structure

Associates with cdc2, res2 and rum1. Interacts with pop1 only when phosphorylated. Ref.1 Ref.8 Ref.9 Ref.10 Ref.11

Subcellular location

Nucleus. Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body Ref.9 Ref.12.

Induction

Highly induced upon nitrogen starvation and during conjugation. Ref.3

Post-translational modification

Phosphorylated. Ref.9

Sequence similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Contains 1 cyclin N-terminal domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Conjugation
Mitosis
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
   Molecular functionCyclin
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processconjugation

Inferred from electronic annotation. Source: UniProtKB-KW

mitotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of induction of conjugation with cellular fusion

Inferred from mutant phenotype PubMed 18059475. Source: PomBase

regulation of G1/S transition of mitotic cell cycle

Inferred from mutant phenotype Ref.6. Source: PomBase

regulation of conjugation with cellular fusion

Inferred from mutant phenotype Ref.3. Source: PomBase

regulation of cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: InterPro

regulation of mitotic cell cycle

Inferred from mutant phenotype Ref.1. Source: PomBase

signal transduction

Non-traceable author statement. Source: PomBase

traversing start control point of mitotic cell cycle

Inferred from genetic interaction Ref.3. Source: PomBase

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

mitotic spindle pole body

Inferred from direct assay Ref.12. Source: PomBase

nucleus

Inferred from direct assay Ref.12. Source: PomBase

   Molecular_functioncyclin-dependent protein serine/threonine kinase regulator activity

Non-traceable author statement. Source: PomBase

protein N-terminus binding

Inferred from physical interaction Ref.10. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.10PubMed 1497023Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411G2/mitotic-specific cyclin cig2
PRO_0000080401

Regions

Domain139 – 265127Cyclin N-terminal
Region181 – 27393Interaction with pop1
Motif51 – 6010Destruction box
Compositional bias394 – 3974Poly-Asp

Experimental info

Mutagenesis511R → A: Improves protein stability; when associated with A-54. Ref.9
Mutagenesis541L → A: Improves protein stability; when associated with A-51. Ref.9
Mutagenesis1691R → A: Prevents binding to cdc2 and improves protein stability; when associated with A-170 and A-171. Ref.9
Mutagenesis1701E → A: Prevents binding to cdc2 and improves protein stability; when associated with A-169 and A-171. Ref.9
Mutagenesis1711I → A: Prevents binding to cdc2 and improves protein stability; when associated with A-169 and A-170. Ref.9
Mutagenesis2681S → N: No effect on protein stability; when associated with A-329. Ref.9
Mutagenesis3291T → A: No effect on protein stability; when associated with N-268. Ref.9
Sequence conflict1 – 4949MALYS…EINLP → MKTHLLANKLYQKGLRRQNY LQICKLICHA in CAA49640. Ref.1
Sequence conflict119 – 1246VSNVDD → ALMLMN in CAA49640. Ref.1
Sequence conflict3151Missing in CAA49640. Ref.1
Sequence conflict3301P → A in CAA49640. Ref.1
Sequence conflict4111H → QRTLL in CAA49640. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P36630 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 6F255D2E26C6F8B3

FASTA41147,477
        10         20         30         40         50         60 
MALYSISKPV GSKINKHSYQ DENTLVGKQA LSKGTEKTKL STNFEINLPR RTVLSDVSNV 

        70         80         90        100        110        120 
GKNNADEKDT KKAKRSFDES NLSTNEEADK PVESKFVKKL KVYSKNADPS VETLQKDRVS 

       130        140        150        160        170        180 
NVDDHLSSNP LMAEEYAPEI FEYIRKLDLK CLPNPKYMDQ QKELTWKMRE ILNEWLVEIH 

       190        200        210        220        230        240 
SNFCLMPETL YLAVNIIDRF LSRRSCSLSK FQLTGITALL IASKYEEVMC PSIQNFVYMT 

       250        260        270        280        290        300 
DGAFTVEDVC VAERYMLNVL NFDLSYPSPL NFLRKISQAE GYDAQTRTLG KYLTEIYLFD 

       310        320        330        340        350        360 
HDLLRYPMSK IAAAAMYLSR RLLRRGPWTP KLVESSGGYE EHELKEIAYI MLHYHNKPLE 

       370        380        390        400        410 
HKAFFQKYSS KRFLKASIFV HQLVRQRYSV NRTDDDDLQS EPSSSLTNDG H 

« Hide

References

« Hide 'large scale' references
[1]"Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis."
Bueno A., Russell P.
Mol. Cell. Biol. 13:2286-2297(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CDC2.
Strain: 972 / ATCC 24843.
[2]Erratum
Bueno A., Russell P.
Mol. Cell. Biol. 14:869-869(1994)
[3]"A B-type cyclin negatively regulates conjugation via interacting with cell cycle 'start' genes in fission yeast."
Obara-Ishihara T., Okayama H.
EMBO J. 13:1863-1872(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
[4]"Interaction between the Cig1 and Cig2 B-type cyclins in the fission yeast cell cycle."
Connolly T., Beach D.
Mol. Cell. Biol. 14:768-776(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[5]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[6]"Cig2, a B-type cyclin, promotes the onset of S in Schizosaccharomyces pombe."
Mondesert O., McGowan C.H., Russell P.
Mol. Cell. Biol. 16:1527-1533(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"A quantitative model for the cdc2 control of S phase and mitosis in fission yeast."
Stern B., Nurse P.
Trends Genet. 12:345-350(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON ASSOCIATION WITH CDC2.
[8]"Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast."
Stern B., Nurse P.
Mol. Biol. Cell 9:1309-1321(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RUM1.
[9]"The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases."
Yamano H., Kitamura K., Kominami K., Lehmann A., Hunt T., Toda T.
Mol. Cell 6:1377-1387(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDC2, SUBCELLULAR LOCATION, PHOSPHORYLATION, DESTRUCTION BOX MOTIF, MUTAGENESIS OF ARG-51; LEU-54; ARG-169; GLU-170; ILE-171; SER-268 AND THR-329.
[10]"Feedback regulation of the MBF transcription factor by cyclin Cig2."
Ayte J., Schweitzer C., Zarzov P., Nurse P., DeCaprio J.A.
Nat. Cell Biol. 3:1043-1050(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RES2.
[11]"Requirement of the SCFPop1/Pop2 ubiquitin ligase for degradation of the fission yeast S phase cyclin Cig2."
Yamano H., Kominami K., Harrison C., Kitamura K., Katayama S., Dhut S., Hunt T., Toda T.
J. Biol. Chem. 279:18974-18980(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH POP1.
[12]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X70046 mRNA. Translation: CAA49640.1.
D28751 mRNA. Translation: BAA05943.1.
S67490 mRNA. Translation: AAB29297.2. Sequence problems.
CU329670 Genomic DNA. Translation: CAC21469.1.
PIRA48100.
S44344.
T52009.
RefSeqNP_593889.1. NM_001019319.2.

3D structure databases

ProteinModelPortalP36630.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid279901. 35 interactions.
IntActP36630. 5 interactions.
MINTMINT-4689261.
STRING4896.SPAPB2B4.03-1.

Proteomic databases

MaxQBP36630.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAPB2B4.03.1; SPAPB2B4.03.1:pep; SPAPB2B4.03.
GeneID2543481.
KEGGspo:SPAPB2B4.03.

Organism-specific databases

PomBaseSPAPB2B4.03.

Phylogenomic databases

eggNOGCOG5024.
HOGENOMHOG000167672.
KOK02220.
OMAKEIAYIM.
OrthoDBEOG7FNCJX.
PhylomeDBP36630.

Family and domain databases

Gene3D1.10.472.10. 2 hits.
InterProIPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFPIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMSSF47954. SSF47954. 2 hits.
PROSITEPS00292. CYCLINS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20804493.
PROP36630.

Entry information

Entry nameCG22_SCHPO
AccessionPrimary (citable) accession number: P36630
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names