Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P36630

- CG22_SCHPO

UniProt

P36630 - CG22_SCHPO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

G2/mitotic-specific cyclin cig2

Gene

cig2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the G2/M and G1/S (mitosis) transition. Interacts with the cdc2 protein kinase to form MPF. Interaction with res2 promotes the phosphorylation of res1 and inhibits MBF-dependent gene transcription. Forms an autoregulating feedback-inhibition loop with MBF which is important for normal regulation of the cell cycle. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis. Negatively regulates conjugation via interacting with cell cycle 'start' genes. Degraded by skp1, pop1 and pop2 in the G2 and M phases of the cell cycle.7 Publications

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase activator activity Source: PomBase

GO - Biological processi

  1. conjugation Source: UniProtKB-KW
  2. mitotic nuclear division Source: UniProtKB-KW
  3. negative regulation of conjugation with cellular fusion Source: PomBase
  4. negative regulation of induction of conjugation with cellular fusion Source: PomBase
  5. positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle Source: PomBase
  6. positive regulation of G1/S transition of mitotic cell cycle Source: PomBase
  7. signal transduction involved in G1 cell size control checkpoint Source: PomBase
  8. traversing start control point of mitotic cell cycle Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Conjugation, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin cig2
Gene namesi
Name:cig2
Synonyms:cyc17
ORF Names:SPAPB2B4.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAPB2B4.03.

Subcellular locationi

GO - Cellular componenti

  1. cyclin-dependent protein kinase holoenzyme complex Source: PomBase
  2. cytoplasm Source: UniProtKB-KW
  3. mitotic spindle pole body Source: PomBase
  4. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi51 – 511R → A: Improves protein stability; when associated with A-54. 1 Publication
Mutagenesisi54 – 541L → A: Improves protein stability; when associated with A-51. 1 Publication
Mutagenesisi169 – 1691R → A: Prevents binding to cdc2 and improves protein stability; when associated with A-170 and A-171. 1 Publication
Mutagenesisi170 – 1701E → A: Prevents binding to cdc2 and improves protein stability; when associated with A-169 and A-171. 1 Publication
Mutagenesisi171 – 1711I → A: Prevents binding to cdc2 and improves protein stability; when associated with A-169 and A-170. 1 Publication
Mutagenesisi268 – 2681S → N: No effect on protein stability; when associated with A-329. 1 Publication
Mutagenesisi329 – 3291T → A: No effect on protein stability; when associated with N-268. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411G2/mitotic-specific cyclin cig2PRO_0000080401Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36630.

Expressioni

Inductioni

Highly induced upon nitrogen starvation and during conjugation.1 Publication

Interactioni

Subunit structurei

Associates with cdc2, res2 and rum1. Interacts with pop1 only when phosphorylated.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
pop1P870604EBI-1149212,EBI-1185389
res1P335203EBI-1149212,EBI-1149288
res2P414123EBI-1149212,EBI-1149177
rum1P403802EBI-1149212,EBI-1187892

Protein-protein interaction databases

BioGridi279901. 36 interactions.
IntActiP36630. 5 interactions.
MINTiMINT-4689261.
STRINGi4896.SPAPB2B4.03-1.

Structurei

3D structure databases

ProteinModelPortaliP36630.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini139 – 265127Cyclin N-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 27393Interaction with pop1Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi51 – 6010Destruction box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi394 – 3974Poly-Asp

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated
Contains 1 cyclin N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG5024.
HOGENOMiHOG000167672.
InParanoidiP36630.
KOiK02220.
OMAiKEIAYIM.
OrthoDBiEOG7FNCJX.
PhylomeDBiP36630.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36630-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALYSISKPV GSKINKHSYQ DENTLVGKQA LSKGTEKTKL STNFEINLPR
60 70 80 90 100
RTVLSDVSNV GKNNADEKDT KKAKRSFDES NLSTNEEADK PVESKFVKKL
110 120 130 140 150
KVYSKNADPS VETLQKDRVS NVDDHLSSNP LMAEEYAPEI FEYIRKLDLK
160 170 180 190 200
CLPNPKYMDQ QKELTWKMRE ILNEWLVEIH SNFCLMPETL YLAVNIIDRF
210 220 230 240 250
LSRRSCSLSK FQLTGITALL IASKYEEVMC PSIQNFVYMT DGAFTVEDVC
260 270 280 290 300
VAERYMLNVL NFDLSYPSPL NFLRKISQAE GYDAQTRTLG KYLTEIYLFD
310 320 330 340 350
HDLLRYPMSK IAAAAMYLSR RLLRRGPWTP KLVESSGGYE EHELKEIAYI
360 370 380 390 400
MLHYHNKPLE HKAFFQKYSS KRFLKASIFV HQLVRQRYSV NRTDDDDLQS
410
EPSSSLTNDG H
Length:411
Mass (Da):47,477
Last modified:February 1, 1995 - v2
Checksum:i6F255D2E26C6F8B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 4949MALYS…EINLP → MKTHLLANKLYQKGLRRQNY LQICKLICHA in CAA49640. (PubMed:8455610)CuratedAdd
BLAST
Sequence conflicti119 – 1246VSNVDD → ALMLMN in CAA49640. (PubMed:8455610)Curated
Sequence conflicti315 – 3151Missing in CAA49640. (PubMed:8455610)Curated
Sequence conflicti330 – 3301P → A in CAA49640. (PubMed:8455610)Curated
Sequence conflicti411 – 4111H → QRTLL in CAA49640. (PubMed:8455610)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70046 mRNA. Translation: CAA49640.1.
D28751 mRNA. Translation: BAA05943.1.
S67490 mRNA. Translation: AAB29297.2. Sequence problems.
CU329670 Genomic DNA. Translation: CAC21469.1.
PIRiA48100.
S44344.
T52009.
RefSeqiNP_593889.1. NM_001019319.2.

Genome annotation databases

EnsemblFungiiSPAPB2B4.03.1; SPAPB2B4.03.1:pep; SPAPB2B4.03.
GeneIDi2543481.
KEGGispo:SPAPB2B4.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70046 mRNA. Translation: CAA49640.1 .
D28751 mRNA. Translation: BAA05943.1 .
S67490 mRNA. Translation: AAB29297.2 . Sequence problems.
CU329670 Genomic DNA. Translation: CAC21469.1 .
PIRi A48100.
S44344.
T52009.
RefSeqi NP_593889.1. NM_001019319.2.

3D structure databases

ProteinModelPortali P36630.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279901. 36 interactions.
IntActi P36630. 5 interactions.
MINTi MINT-4689261.
STRINGi 4896.SPAPB2B4.03-1.

Proteomic databases

MaxQBi P36630.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAPB2B4.03.1 ; SPAPB2B4.03.1:pep ; SPAPB2B4.03 .
GeneIDi 2543481.
KEGGi spo:SPAPB2B4.03.

Organism-specific databases

PomBasei SPAPB2B4.03.

Phylogenomic databases

eggNOGi COG5024.
HOGENOMi HOG000167672.
InParanoidi P36630.
KOi K02220.
OMAi KEIAYIM.
OrthoDBi EOG7FNCJX.
PhylomeDBi P36630.

Miscellaneous databases

NextBioi 20804493.
PROi P36630.

Family and domain databases

Gene3Di 1.10.472.10. 2 hits.
InterProi IPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view ]
Pfami PF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTi SM00385. CYCLIN. 2 hits.
[Graphical view ]
SUPFAMi SSF47954. SSF47954. 2 hits.
PROSITEi PS00292. CYCLINS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis."
    Bueno A., Russell P.
    Mol. Cell. Biol. 13:2286-2297(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CDC2.
    Strain: 972 / ATCC 24843.
  2. Erratum
    Bueno A., Russell P.
    Mol. Cell. Biol. 14:869-869(1994)
  3. "A B-type cyclin negatively regulates conjugation via interacting with cell cycle 'start' genes in fission yeast."
    Obara-Ishihara T., Okayama H.
    EMBO J. 13:1863-1872(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
  4. "Interaction between the Cig1 and Cig2 B-type cyclins in the fission yeast cell cycle."
    Connolly T., Beach D.
    Mol. Cell. Biol. 14:768-776(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  5. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  6. "Cig2, a B-type cyclin, promotes the onset of S in Schizosaccharomyces pombe."
    Mondesert O., McGowan C.H., Russell P.
    Mol. Cell. Biol. 16:1527-1533(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "A quantitative model for the cdc2 control of S phase and mitosis in fission yeast."
    Stern B., Nurse P.
    Trends Genet. 12:345-350(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON ASSOCIATION WITH CDC2.
  8. "Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast."
    Stern B., Nurse P.
    Mol. Biol. Cell 9:1309-1321(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RUM1.
  9. "The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases."
    Yamano H., Kitamura K., Kominami K., Lehmann A., Hunt T., Toda T.
    Mol. Cell 6:1377-1387(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC2, SUBCELLULAR LOCATION, PHOSPHORYLATION, DESTRUCTION BOX MOTIF, MUTAGENESIS OF ARG-51; LEU-54; ARG-169; GLU-170; ILE-171; SER-268 AND THR-329.
  10. "Feedback regulation of the MBF transcription factor by cyclin Cig2."
    Ayte J., Schweitzer C., Zarzov P., Nurse P., DeCaprio J.A.
    Nat. Cell Biol. 3:1043-1050(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RES2.
  11. "Requirement of the SCFPop1/Pop2 ubiquitin ligase for degradation of the fission yeast S phase cyclin Cig2."
    Yamano H., Kominami K., Harrison C., Kitamura K., Katayama S., Dhut S., Hunt T., Toda T.
    J. Biol. Chem. 279:18974-18980(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POP1.
  12. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCG22_SCHPO
AccessioniPrimary (citable) accession number: P36630
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3