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P36630

- CG22_SCHPO

UniProt

P36630 - CG22_SCHPO

Protein

G2/mitotic-specific cyclin cig2

Gene

cig2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Essential for the control of the cell cycle at the G2/M and G1/S (mitosis) transition. Interacts with the cdc2 protein kinase to form MPF. Interaction with res2 promotes the phosphorylation of res1 and inhibits MBF-dependent gene transcription. Forms an autoregulating feedback-inhibition loop with MBF which is important for normal regulation of the cell cycle. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis. Negatively regulates conjugation via interacting with cell cycle 'start' genes. Degraded by skp1, pop1 and pop2 in the G2 and M phases of the cell cycle.7 Publications

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase regulator activity Source: PomBase
    2. protein binding Source: UniProtKB
    3. protein N-terminus binding Source: UniProtKB

    GO - Biological processi

    1. conjugation Source: UniProtKB-KW
    2. mitotic nuclear division Source: UniProtKB-KW
    3. negative regulation of induction of conjugation with cellular fusion Source: PomBase
    4. regulation of conjugation with cellular fusion Source: PomBase
    5. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
    6. regulation of G1/S transition of mitotic cell cycle Source: PomBase
    7. regulation of mitotic cell cycle Source: PomBase
    8. signal transduction Source: PomBase
    9. traversing start control point of mitotic cell cycle Source: PomBase

    Keywords - Molecular functioni

    Cyclin

    Keywords - Biological processi

    Cell cycle, Cell division, Conjugation, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_188254. Condensation of Prometaphase Chromosomes.
    REACT_188278. MASTL Facilitates Mitotic Progression.
    REACT_199398. Cyclin A/B1 associated events during G2/M transition.
    REACT_219807. Resolution of Sister Chromatid Cohesion.
    REACT_219991. Cyclin B2 mediated events.
    REACT_221258. Depolymerisation of the Nuclear Lamina.
    REACT_222028. Regulation of APC/C activators between G1/S and early anaphase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G2/mitotic-specific cyclin cig2
    Gene namesi
    Name:cig2
    Synonyms:cyc17
    ORF Names:SPAPB2B4.03
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAPB2B4.03.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. mitotic spindle pole body Source: PomBase
    3. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi51 – 511R → A: Improves protein stability; when associated with A-54. 1 Publication
    Mutagenesisi54 – 541L → A: Improves protein stability; when associated with A-51. 1 Publication
    Mutagenesisi169 – 1691R → A: Prevents binding to cdc2 and improves protein stability; when associated with A-170 and A-171. 1 Publication
    Mutagenesisi170 – 1701E → A: Prevents binding to cdc2 and improves protein stability; when associated with A-169 and A-171. 1 Publication
    Mutagenesisi171 – 1711I → A: Prevents binding to cdc2 and improves protein stability; when associated with A-169 and A-170. 1 Publication
    Mutagenesisi268 – 2681S → N: No effect on protein stability; when associated with A-329. 1 Publication
    Mutagenesisi329 – 3291T → A: No effect on protein stability; when associated with N-268. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 411411G2/mitotic-specific cyclin cig2PRO_0000080401Add
    BLAST

    Post-translational modificationi

    Phosphorylated.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP36630.

    Expressioni

    Inductioni

    Highly induced upon nitrogen starvation and during conjugation.1 Publication

    Interactioni

    Subunit structurei

    Associates with cdc2, res2 and rum1. Interacts with pop1 only when phosphorylated.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    pop1P870604EBI-1149212,EBI-1185389
    res1P335203EBI-1149212,EBI-1149288
    res2P414123EBI-1149212,EBI-1149177
    rum1P403802EBI-1149212,EBI-1187892

    Protein-protein interaction databases

    BioGridi279901. 35 interactions.
    IntActiP36630. 5 interactions.
    MINTiMINT-4689261.
    STRINGi4896.SPAPB2B4.03-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP36630.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini139 – 265127Cyclin N-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni181 – 27393Interaction with pop1Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi51 – 6010Destruction box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi394 – 3974Poly-Asp

    Sequence similaritiesi

    Belongs to the cyclin family. Cyclin AB subfamily.Curated
    Contains 1 cyclin N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG5024.
    HOGENOMiHOG000167672.
    KOiK02220.
    OMAiKEIAYIM.
    OrthoDBiEOG7FNCJX.
    PhylomeDBiP36630.

    Family and domain databases

    Gene3Di1.10.472.10. 2 hits.
    InterProiIPR013763. Cyclin-like.
    IPR014400. Cyclin_A/B/D/E/F.
    IPR004367. Cyclin_C-dom.
    IPR006671. Cyclin_N.
    [Graphical view]
    PfamiPF02984. Cyclin_C. 1 hit.
    PF00134. Cyclin_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001771. Cyclin_A_B_D_E. 1 hit.
    SMARTiSM00385. CYCLIN. 2 hits.
    [Graphical view]
    SUPFAMiSSF47954. SSF47954. 2 hits.
    PROSITEiPS00292. CYCLINS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36630-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALYSISKPV GSKINKHSYQ DENTLVGKQA LSKGTEKTKL STNFEINLPR    50
    RTVLSDVSNV GKNNADEKDT KKAKRSFDES NLSTNEEADK PVESKFVKKL 100
    KVYSKNADPS VETLQKDRVS NVDDHLSSNP LMAEEYAPEI FEYIRKLDLK 150
    CLPNPKYMDQ QKELTWKMRE ILNEWLVEIH SNFCLMPETL YLAVNIIDRF 200
    LSRRSCSLSK FQLTGITALL IASKYEEVMC PSIQNFVYMT DGAFTVEDVC 250
    VAERYMLNVL NFDLSYPSPL NFLRKISQAE GYDAQTRTLG KYLTEIYLFD 300
    HDLLRYPMSK IAAAAMYLSR RLLRRGPWTP KLVESSGGYE EHELKEIAYI 350
    MLHYHNKPLE HKAFFQKYSS KRFLKASIFV HQLVRQRYSV NRTDDDDLQS 400
    EPSSSLTNDG H 411
    Length:411
    Mass (Da):47,477
    Last modified:February 1, 1995 - v2
    Checksum:i6F255D2E26C6F8B3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 4949MALYS…EINLP → MKTHLLANKLYQKGLRRQNY LQICKLICHA in CAA49640. (PubMed:8455610)CuratedAdd
    BLAST
    Sequence conflicti119 – 1246VSNVDD → ALMLMN in CAA49640. (PubMed:8455610)Curated
    Sequence conflicti315 – 3151Missing in CAA49640. (PubMed:8455610)Curated
    Sequence conflicti330 – 3301P → A in CAA49640. (PubMed:8455610)Curated
    Sequence conflicti411 – 4111H → QRTLL in CAA49640. (PubMed:8455610)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70046 mRNA. Translation: CAA49640.1.
    D28751 mRNA. Translation: BAA05943.1.
    S67490 mRNA. Translation: AAB29297.2. Sequence problems.
    CU329670 Genomic DNA. Translation: CAC21469.1.
    PIRiA48100.
    S44344.
    T52009.
    RefSeqiNP_593889.1. NM_001019319.2.

    Genome annotation databases

    EnsemblFungiiSPAPB2B4.03.1; SPAPB2B4.03.1:pep; SPAPB2B4.03.
    GeneIDi2543481.
    KEGGispo:SPAPB2B4.03.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70046 mRNA. Translation: CAA49640.1 .
    D28751 mRNA. Translation: BAA05943.1 .
    S67490 mRNA. Translation: AAB29297.2 . Sequence problems.
    CU329670 Genomic DNA. Translation: CAC21469.1 .
    PIRi A48100.
    S44344.
    T52009.
    RefSeqi NP_593889.1. NM_001019319.2.

    3D structure databases

    ProteinModelPortali P36630.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279901. 35 interactions.
    IntActi P36630. 5 interactions.
    MINTi MINT-4689261.
    STRINGi 4896.SPAPB2B4.03-1.

    Proteomic databases

    MaxQBi P36630.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAPB2B4.03.1 ; SPAPB2B4.03.1:pep ; SPAPB2B4.03 .
    GeneIDi 2543481.
    KEGGi spo:SPAPB2B4.03.

    Organism-specific databases

    PomBasei SPAPB2B4.03.

    Phylogenomic databases

    eggNOGi COG5024.
    HOGENOMi HOG000167672.
    KOi K02220.
    OMAi KEIAYIM.
    OrthoDBi EOG7FNCJX.
    PhylomeDBi P36630.

    Enzyme and pathway databases

    Reactomei REACT_188254. Condensation of Prometaphase Chromosomes.
    REACT_188278. MASTL Facilitates Mitotic Progression.
    REACT_199398. Cyclin A/B1 associated events during G2/M transition.
    REACT_219807. Resolution of Sister Chromatid Cohesion.
    REACT_219991. Cyclin B2 mediated events.
    REACT_221258. Depolymerisation of the Nuclear Lamina.
    REACT_222028. Regulation of APC/C activators between G1/S and early anaphase.

    Miscellaneous databases

    NextBioi 20804493.
    PROi P36630.

    Family and domain databases

    Gene3Di 1.10.472.10. 2 hits.
    InterProi IPR013763. Cyclin-like.
    IPR014400. Cyclin_A/B/D/E/F.
    IPR004367. Cyclin_C-dom.
    IPR006671. Cyclin_N.
    [Graphical view ]
    Pfami PF02984. Cyclin_C. 1 hit.
    PF00134. Cyclin_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001771. Cyclin_A_B_D_E. 1 hit.
    SMARTi SM00385. CYCLIN. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47954. SSF47954. 2 hits.
    PROSITEi PS00292. CYCLINS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis."
      Bueno A., Russell P.
      Mol. Cell. Biol. 13:2286-2297(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CDC2.
      Strain: 972 / ATCC 24843.
    2. Erratum
      Bueno A., Russell P.
      Mol. Cell. Biol. 14:869-869(1994)
    3. "A B-type cyclin negatively regulates conjugation via interacting with cell cycle 'start' genes in fission yeast."
      Obara-Ishihara T., Okayama H.
      EMBO J. 13:1863-1872(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
    4. "Interaction between the Cig1 and Cig2 B-type cyclins in the fission yeast cell cycle."
      Connolly T., Beach D.
      Mol. Cell. Biol. 14:768-776(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    5. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    6. "Cig2, a B-type cyclin, promotes the onset of S in Schizosaccharomyces pombe."
      Mondesert O., McGowan C.H., Russell P.
      Mol. Cell. Biol. 16:1527-1533(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A quantitative model for the cdc2 control of S phase and mitosis in fission yeast."
      Stern B., Nurse P.
      Trends Genet. 12:345-350(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON ASSOCIATION WITH CDC2.
    8. "Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast."
      Stern B., Nurse P.
      Mol. Biol. Cell 9:1309-1321(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RUM1.
    9. "The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases."
      Yamano H., Kitamura K., Kominami K., Lehmann A., Hunt T., Toda T.
      Mol. Cell 6:1377-1387(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC2, SUBCELLULAR LOCATION, PHOSPHORYLATION, DESTRUCTION BOX MOTIF, MUTAGENESIS OF ARG-51; LEU-54; ARG-169; GLU-170; ILE-171; SER-268 AND THR-329.
    10. "Feedback regulation of the MBF transcription factor by cyclin Cig2."
      Ayte J., Schweitzer C., Zarzov P., Nurse P., DeCaprio J.A.
      Nat. Cell Biol. 3:1043-1050(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RES2.
    11. "Requirement of the SCFPop1/Pop2 ubiquitin ligase for degradation of the fission yeast S phase cyclin Cig2."
      Yamano H., Kominami K., Harrison C., Kitamura K., Katayama S., Dhut S., Hunt T., Toda T.
      J. Biol. Chem. 279:18974-18980(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH POP1.
    12. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCG22_SCHPO
    AccessioniPrimary (citable) accession number: P36630
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3