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Protein

Meiotic recombination protein rec8

Gene

rec8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved primarily in the early steps of meiotic recombination. Required to ensure reductional chromosome segregation.2 Publications

GO - Biological processi

  1. attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation Source: PomBase
  2. meiotic sister chromatid cohesion, centromeric Source: PomBase
  3. meiotic sister chromatid cohesion involved in meiosis I Source: PomBase
  4. synapsis Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Meiosis

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein rec8
Alternative name(s):
Cohesin rec8
Gene namesi
Name:rec8
ORF Names:SPBC29A10.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC29A10.14.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. condensed nuclear chromosome inner kinetochore Source: PomBase
  2. horsetail nucleus Source: PomBase
  3. nuclear meiotic cohesin complex Source: PomBase
  4. nuclear pericentric heterochromatin Source: PomBase
  5. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Meiotic recombination protein rec8PRO_0000097880Add
BLAST

Post-translational modificationi

Phosphorylated during prophase until after meiosis I.2 Publications

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Abundant at 3 hours after induction of meiosis but undetectable before induction or at 4 hours and later.

Interactioni

Subunit structurei

Interacts with moa1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
moa1Q9UTI42EBI-1564281,EBI-1564258

Protein-protein interaction databases

BioGridi276986. 14 interactions.
IntActiP36626. 1 interaction.
MINTiMINT-4689209.
STRINGi4896.SPBC29A10.14-1.

Structurei

3D structure databases

ProteinModelPortaliP36626.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the rad21 family.Curated

Phylogenomic databases

eggNOGiNOG42761.
InParanoidiP36626.
KOiK12780.
OrthoDBiEOG7KSXM4.
PhylomeDBiP36626.

Family and domain databases

Gene3Di1.10.10.580. 1 hit.
InterProiIPR023093. Rad21/Rec8_C.
IPR006909. Rad21/Rec8_C_eu.
IPR006910. Rad21_Rec8_N.
[Graphical view]
PfamiPF04824. Rad21_Rec8. 1 hit.
PF04825. Rad21_Rec8_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36626-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFYNQDVLTK EKGGMGVIWL AATLGSKHSL RKLHKKDIMS VDIDEACDFV
60 70 80 90 100
AFSPEPLALR LSSNLMIGVT RVWAHQYSFF HSQVSTLHLR VRKELDHFTS
110 120 130 140 150
KPFKNIDIQN EQTNPKQLLL AEDPAFIPEV SLYDAFNLPS VDLHVDMSSF
160 170 180 190 200
TQPKENPNIS VLETLPDSTS YLINTSQNYS LRNNVSSFVY EDSRAFSTEE
210 220 230 240 250
PLDFEFDENG DIQELTKGTI NSDPSLQAAS QHSNLGSVQR EYNSEEQESR
260 270 280 290 300
IHMFEIDEDV LPLPVPLQSV MDSEHNENEP RALKRRKVQK LLEPDENIEL
310 320 330 340 350
STRTLSQWRK NYVERMIALE ATKYVRRRGA SSAKKKELNK FFDWESFHPL
360 370 380 390 400
LKPWIEKLKP SNNTPSEIDD VLRNIDTSEV EVGRDVQGEL GLNIPWNTSS
410 420 430 440 450
RSNSAINSKS HSQTGSEHST PLLDTKYRKR LPHSPSMPSR VEFLPALESS
460 470 480 490 500
QFHETLNSEL SLQLSDDFVL YKNTQEENAH LMLSMEKECA NFYEYAKTAI
510 520 530 540 550
YENNGRITFS SLLPNDLKRP VVAQAFSHLL SLATKSAFLV KQDKPYSEIS
560
VSLNLKSTDA I
Length:561
Mass (Da):64,011
Last modified:January 11, 2001 - v2
Checksum:iE6B9B3A02143D50A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti531 – 56131SLATK…STDAI → CKYHNQTTN in AAB04034. (PubMed:1339382)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223299 Genomic DNA. Translation: CAA11240.1.
AB018077 mRNA. Translation: BAA33496.1.
CU329671 Genomic DNA. Translation: CAA22442.1.
M85298 Genomic DNA. Translation: AAB04034.1.
PIRiT43428.
RefSeqiNP_596059.1. NM_001021970.2.

Genome annotation databases

EnsemblFungiiSPBC29A10.14.1; SPBC29A10.14.1:pep; SPBC29A10.14.
GeneIDi2540458.
KEGGispo:SPBC29A10.14.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223299 Genomic DNA. Translation: CAA11240.1.
AB018077 mRNA. Translation: BAA33496.1.
CU329671 Genomic DNA. Translation: CAA22442.1.
M85298 Genomic DNA. Translation: AAB04034.1.
PIRiT43428.
RefSeqiNP_596059.1. NM_001021970.2.

3D structure databases

ProteinModelPortaliP36626.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276986. 14 interactions.
IntActiP36626. 1 interaction.
MINTiMINT-4689209.
STRINGi4896.SPBC29A10.14-1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC29A10.14.1; SPBC29A10.14.1:pep; SPBC29A10.14.
GeneIDi2540458.
KEGGispo:SPBC29A10.14.

Organism-specific databases

PomBaseiSPBC29A10.14.

Phylogenomic databases

eggNOGiNOG42761.
InParanoidiP36626.
KOiK12780.
OrthoDBiEOG7KSXM4.
PhylomeDBiP36626.

Miscellaneous databases

NextBioi20801585.

Family and domain databases

Gene3Di1.10.10.580. 1 hit.
InterProiIPR023093. Rad21/Rec8_C.
IPR006909. Rad21/Rec8_C_eu.
IPR006910. Rad21_Rec8_N.
[Graphical view]
PfamiPF04824. Rad21_Rec8. 1 hit.
PF04825. Rad21_Rec8_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans."
    Parisi S., McKay M.J., Molnar M., Thompson M.A., van der Spek P.J., van Drunen-Schoenmaker E., Kanaar R., Lehmann E., Hoeijmakers J.H.J., Kohli J.
    Mol. Cell. Biol. 19:3515-3528(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, PHOSPHORYLATION.
  2. "Cohesin Rec8 is required for reductional chromosome segregation at meiosis."
    Watanabe Y., Nurse P.
    Nature 400:461-464(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Meiotically induced rec7 and rec8 genes of Schizosaccharomyces pombe."
    Lin Y., Larson K.L., Dorer R., Smith G.R.
    Genetics 132:75-85(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 147-561.
  5. "The kinetochore protein moa1 enables cohesion-mediated monopolar attachment at meiosis I."
    Yokobayashi S., Watanabe Y.
    Cell 123:803-817(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MOA1.
  6. "A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events."
    Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L., Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P., Smith G.R., Moreno S.
    Curr. Biol. 15:2056-2062(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MEIOSIS.
  7. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiREC8_SCHPO
AccessioniPrimary (citable) accession number: P36626
Secondary accession number(s): O60051
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: February 4, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.