P36626 (REC8_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Meiotic recombination protein rec8

Alternative name(s):
Cohesin rec8

Gene names

Name:	rec8
ORF Names:	SPBC29A10.14

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved primarily in the early steps of meiotic recombination. Required to ensure reductional chromosome segregation. Ref.2 Ref.6
Subunit structure	Interacts with moa1. Ref.5
Subcellular location	Nucleus Ref.1 Ref.7.
Developmental stage	Abundant at 3 hours after induction of meiosis but undetectable before induction or at 4 hours and later.
Post-translational modification	Phosphorylated during prophase until after meiosis I. Ref.1 Ref.2
Sequence similarities	Belongs to the rad21 family.

Ontologies

Keywords
Biological process	Meiosis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation Inferred from mutant phenotype PubMed 12748297Ref.5. Source: PomBase linear element assembly Inferred from mutant phenotype PubMed 8536990. Source: PomBase meiotic DNA double-strand break formation Inferred from mutant phenotype PubMed 16043696. Source: PomBase meiotic sister chromatid segregation Inferred from direct assay PubMed 14532136. Source: PomBase reciprocal meiotic recombination Inferred from mutant phenotype PubMed 8299939. Source: PomBase
Cellular_component	condensed chromosome inner kinetochore Inferred from direct assay PubMed 11201746. Source: PomBase meiotic cohesin complex Inferred from direct assay PubMed 12750522. Source: PomBase nuclear chromosome Inferred by curator. Source: PomBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
moa1	Q9UTI4	2	EBI-1564281,EBI-1564258

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Meiotic recombination protein rec8		PRO_0000097880
Experimental info
	Sequence conflict	531 – 561	31	SLATK…STDAI → CKYHNQTTN in AAB04034. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36626 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: E6B9B3A02143D50A	FASTA	561	64,011
	10 20 30 40 50 60 MFYNQDVLTK EKGGMGVIWL AATLGSKHSL RKLHKKDIMS VDIDEACDFV AFSPEPLALR 70 80 90 100 110 120 LSSNLMIGVT RVWAHQYSFF HSQVSTLHLR VRKELDHFTS KPFKNIDIQN EQTNPKQLLL 130 140 150 160 170 180 AEDPAFIPEV SLYDAFNLPS VDLHVDMSSF TQPKENPNIS VLETLPDSTS YLINTSQNYS 190 200 210 220 230 240 LRNNVSSFVY EDSRAFSTEE PLDFEFDENG DIQELTKGTI NSDPSLQAAS QHSNLGSVQR 250 260 270 280 290 300 EYNSEEQESR IHMFEIDEDV LPLPVPLQSV MDSEHNENEP RALKRRKVQK LLEPDENIEL 310 320 330 340 350 360 STRTLSQWRK NYVERMIALE ATKYVRRRGA SSAKKKELNK FFDWESFHPL LKPWIEKLKP 370 380 390 400 410 420 SNNTPSEIDD VLRNIDTSEV EVGRDVQGEL GLNIPWNTSS RSNSAINSKS HSQTGSEHST 430 440 450 460 470 480 PLLDTKYRKR LPHSPSMPSR VEFLPALESS QFHETLNSEL SLQLSDDFVL YKNTQEENAH 490 500 510 520 530 540 LMLSMEKECA NFYEYAKTAI YENNGRITFS SLLPNDLKRP VVAQAFSHLL SLATKSAFLV 550 560 KQDKPYSEIS VSLNLKSTDA I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans." Parisi S., McKay M.J., Molnar M., Thompson M.A., van der Spek P.J., van Drunen-Schoenmaker E., Kanaar R., Lehmann E., Hoeijmakers J.H.J., Kohli J. Mol. Cell. Biol. 19:3515-3528(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, PHOSPHORYLATION.
[2]	"Cohesin Rec8 is required for reductional chromosome segregation at meiosis." Watanabe Y., Nurse P. Nature 400:461-464(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION.
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[4]	"Meiotically induced rec7 and rec8 genes of Schizosaccharomyces pombe." Lin Y., Larson K.L., Dorer R., Smith G.R. Genetics 132:75-85(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 147-561.
[5]	"The kinetochore protein moa1 enables cohesion-mediated monopolar attachment at meiosis I." Yokobayashi S., Watanabe Y. Cell 123:803-817(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MOA1.
[6]	"A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events." Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L., Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P., Smith G.R., Moreno S. Curr. Biol. 15:2056-2062(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN MEIOSIS.
[7]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ223299 Genomic DNA. Translation: CAA11240.1. AB018077 mRNA. Translation: BAA33496.1. CU329671 Genomic DNA. Translation: CAA22442.1. M85298 Genomic DNA. Translation: AAB04034.1.
PIR	T43428.
RefSeq	NP_596059.1. NM_001021970.2.
3D structure databases
ProteinModelPortal	P36626.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36626. 1 interaction.
STRING	4896.SPBC29A10.14-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC29A10.14.1; SPBC29A10.14.1:pep; SPBC29A10.14.
GeneID	2540458.
KEGG	spo:SPBC29A10.14.
Organism-specific databases
PomBase	SPBC29A10.14.
Phylogenomic databases
eggNOG	NOG42761.
KO	K12780.
OrthoDB	EOG45F0XT.
Family and domain databases
Gene3D	1.10.10.580. 1 hit.
InterPro	IPR023093. Rad21/Rec8_C. IPR006909. Rad21/Rec8_C_eu. IPR006910. Rad21_Rec8_N. [Graphical view]
Pfam	PF04824. Rad21_Rec8. 1 hit. PF04825. Rad21_Rec8_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801585.

Entry information

Entry name

REC8_SCHPO

Accession

Primary (citable) accession number: P36626
Secondary accession number(s): O60051

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 11, 2001
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program