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Protein

Leptomycin B resistance protein pmd1

Gene

pmd1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

May be a transmembrane transporter of the mating factor, namely P-factor or M-factor. Confers resistance to leptomycin B and to several other antifungal drugs.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi455 – 4628ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1154 – 11618ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • drug transmembrane transport Source: PomBase
  • response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-159418. Recycling of bile acids and salts.
R-SPO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-SPO-382556. ABC-family proteins mediated transport.

Protein family/group databases

TCDBi3.A.1.201.18. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Leptomycin B resistance protein pmd1
Gene namesi
Name:pmd1
ORF Names:SPCC663.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC663.03.
PomBaseiSPCC663.03. pmd1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9191CytoplasmicSequence analysisAdd
BLAST
Transmembranei92 – 11524HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei138 – 16225HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei220 – 23718HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei244 – 26421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei320 – 34627HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei354 – 37421HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini375 – 788414CytoplasmicSequence analysisAdd
BLAST
Transmembranei789 – 80921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei835 – 85925HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei916 – 93520HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei940 – 95718HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei1022 – 104019HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei1054 – 107219HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini1073 – 1362290CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: PomBase
  • vacuole Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13621362Leptomycin B resistance protein pmd1PRO_0000093452Add
BLAST

Proteomic databases

MaxQBiP36619.

Interactioni

Protein-protein interaction databases

BioGridi275293. 5 interactions.
MINTiMINT-4689100.

Structurei

3D structure databases

ProteinModelPortaliP36619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini95 – 385291ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini420 – 665246ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini795 – 1083289ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1119 – 1356238ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiP36619.
KOiK05658.
OMAiCESTSAI.
OrthoDBiEOG7V76FP.
PhylomeDBiP36619.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLHSKKSTS TVKDNEHSLD LSIKSIPSNE KNFSTEKSEN EASESHVVDV
60 70 80 90 100
VKDPFEQYTP EEQEILYKQI NDTPAKLSGY PRILSYADKW DIMLQLAGTI
110 120 130 140 150
TGIGAGLGMP LMSLVSGQLA QAFTDLASGK GASSFQHTVD HFCLYFIYIA
160 170 180 190 200
IGVFGCSYIY TVTFIIAGER IARRIRQDYL HAILSQNIGY FDRLGAGEIT
210 220 230 240 250
TRITTDTNFI QDGLGEKVGL VFFAIATFVS GFVIAFIRHW KFTLILSSMF
260 270 280 290 300
PAICGGIGLG VPFITKNTKG QIAVVAESST FVEEVFSNIR NAFAFGTQDI
310 320 330 340 350
LAKLYNKYLI TAQRFGINKA IAMGLMVGWM FFVAYGVYGL AFWEGGRLLH
360 370 380 390 400
AGDLDVSKLI GCFFAVLIAS YSLANISPKM QSFVSCASAA KKIFDTIDRV
410 420 430 440 450
SPINAFTPTG DVVKDIKGEI ELKNIRFVYP TRPEVLVLDN FSLVCPSGKI
460 470 480 490 500
TALVGASGSG KSTIIGLVER FYDPIGGQVF LDGKDLRTLN VASLRNQISL
510 520 530 540 550
VQQEPVLFAT TVFENITYGL PDTIKGTLSK EELERRVYDA AKLANAYDFI
560 570 580 590 600
MTLPEQFSTN VGQRGFLMSG GQKQRIAIAR AVISDPKILL LDEATSALDS
610 620 630 640 650
KSEVLVQKAL DNASRSRTTI VIAHRLSTIR NADNIVVVNA GKIVEQGSHN
660 670 680 690 700
ELLDLNGAYA RLVEAQKLSG GEKDQEMVEE ELEDAPREIP ITSFGDDDED
710 720 730 740 750
NDMASLEAPM MSHNTDTDTL NNKLNEKDNV VFEDKTLQHV ASEIVPNLPP
760 770 780 790 800
ADVGELNEEP KKSKKSKKNN HEINSLTALW FIHSFVRTMI EIICLLIGIL
810 820 830 840 850
ASMICGAAYP VQAAVFARFL NIFTDLSSTD FLHKVNVFAV YWLILAIVQF
860 870 880 890 900
FAYAISNFAM TYAMEAVLQR IRYHLFRTLL RQDVEFFDRS ENTVGAITTS
910 920 930 940 950
LSTKIQSLEG LSGPTLGTFF QILTNIISVT ILSLATGWKL GLVTLSTSPV
960 970 980 990 1000
IITAGYYRVR ALDQVQEKLS AAYKESAAFA CESTSAIRTV ASLNREENVF
1010 1020 1030 1040 1050
AEYCDSLIKP GRESAIASLK SGLFFSAAQG VTFLINALTF WYGSTLMRKG
1060 1070 1080 1090 1100
EYNIVQFYTC FIAIVFGIQQ AGQFFGYSAD VTKAKAAAGE IKYLSESKPK
1110 1120 1130 1140 1150
IDTWSTEGKK VESLQSAAIE FRQVEFSYPT RRHIKVLRGL NLTVKPGQFV
1160 1170 1180 1190 1200
AFVGSSGCGK STTIGLIERF YDCDNGAVLV DGVNVRDYNI NDYRKQIALV
1210 1220 1230 1240 1250
SQEPTLYQGT VRENIVLGAS KDVSEEEMIE ACKKANIHEF ILGLPNGYNT
1260 1270 1280 1290 1300
LCGQKGSSLS GGQKQRIAIA RALIRNPKIL LLDEATSALD SHSEKVVQEA
1310 1320 1330 1340 1350
LNAASQGRTT VAIAHRLSSI QDADCIFVFD GGVIAEAGTH AELVKQRGRY
1360
YELVVEQGLN KA
Length:1,362
Mass (Da):149,632
Last modified:January 11, 2001 - v2
Checksum:i2ADF8A8E7B3ACEE8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1334 – 13352IA → TC in BAA01537 (PubMed:1349418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10695 Genomic DNA. Translation: BAA01537.1.
CU329672 Genomic DNA. Translation: CAA20363.1.
PIRiT41534.
RefSeqiNP_588265.1. NM_001023255.2.

Genome annotation databases

EnsemblFungiiSPCC663.03.1; SPCC663.03.1:pep; SPCC663.03.
GeneIDi2538709.
KEGGispo:SPCC663.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10695 Genomic DNA. Translation: BAA01537.1.
CU329672 Genomic DNA. Translation: CAA20363.1.
PIRiT41534.
RefSeqiNP_588265.1. NM_001023255.2.

3D structure databases

ProteinModelPortaliP36619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275293. 5 interactions.
MINTiMINT-4689100.

Protein family/group databases

TCDBi3.A.1.201.18. the atp-binding cassette (abc) superfamily.

Proteomic databases

MaxQBiP36619.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC663.03.1; SPCC663.03.1:pep; SPCC663.03.
GeneIDi2538709.
KEGGispo:SPCC663.03.

Organism-specific databases

EuPathDBiFungiDB:SPCC663.03.
PomBaseiSPCC663.03. pmd1.

Phylogenomic databases

InParanoidiP36619.
KOiK05658.
OMAiCESTSAI.
OrthoDBiEOG7V76FP.
PhylomeDBiP36619.

Enzyme and pathway databases

ReactomeiR-SPO-159418. Recycling of bile acids and salts.
R-SPO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-SPO-382556. ABC-family proteins mediated transport.

Miscellaneous databases

PROiP36619.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A leptomycin B resistance gene of Schizosaccharomyces pombe encodes a protein similar to the mammalian P-glycoproteins."
    Nishi K., Yoshida M., Nishimura M., Nishikawa M., Nishiyama M., Horinouchi S., Beppu T.
    Mol. Microbiol. 6:761-769(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiPMD1_SCHPO
AccessioniPrimary (citable) accession number: P36619
Secondary accession number(s): O74513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: June 8, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.