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P36614

- PPE1_SCHPO

UniProt

P36614 - PPE1_SCHPO

Protein

Serine/threonine-protein phosphatase ppe1

Gene

ppe1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Has a role in chromosome segregation. May provide a dynamic connection between kinetochore microtubules and kinetochore chromatin. Negatively regulates mis12.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi51 – 511Manganese 1By similarity
    Metal bindingi53 – 531Manganese 1By similarity
    Metal bindingi79 – 791Manganese 1By similarity
    Metal bindingi79 – 791Manganese 2By similarity
    Metal bindingi111 – 1111Manganese 2By similarity
    Active sitei112 – 1121Proton donorBy similarity
    Metal bindingi161 – 1611Manganese 2By similarity
    Metal bindingi235 – 2351Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: PomBase
    3. protein binding Source: IntAct

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. mitotic nuclear division Source: UniProtKB-KW
    3. positive regulation of hydroxymethylglutaryl-CoA reductase (NADPH) activity Source: PomBase
    4. positive regulation of isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: PomBase
    5. positive regulation of protein dephosphorylation Source: PomBase
    6. regulation of cell shape Source: PomBase
    7. regulation of mitotic cell cycle Source: PomBase
    8. regulation of mitotic sister chromatid segregation Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Cell shape, Mitosis

    Keywords - Ligandi

    Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase ppe1 (EC:3.1.3.16)
    Alternative name(s):
    Phosphatase esp1
    Gene namesi
    Name:ppe1
    Synonyms:esp1, ppx1
    ORF Names:SPCC1739.12
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome III

    Organism-specific databases

    PomBaseiSPCC1739.12.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Associated with chromatin.

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. nuclear chromatin Source: PomBase
    3. nucleus Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 305305Serine/threonine-protein phosphatase ppe1PRO_0000058882Add
    BLAST

    Proteomic databases

    MaxQBiP36614.

    Interactioni

    Subunit structurei

    Interacts with sts5, ekc1 and mis12.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ekc1O745112EBI-1153118,EBI-1153096

    Protein-protein interaction databases

    BioGridi275341. 32 interactions.
    IntActiP36614. 2 interactions.
    MINTiMINT-4689011.
    STRINGi4896.SPCC1739.12-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP36614.
    SMRiP36614. Positions 3-284.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172696.
    KOiK15427.
    OMAiGELLCVH.
    OrthoDBiEOG7FFN29.
    PhylomeDBiP36614.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36614-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFDLDEWIAT VRKCKYLPEH QLKRLCEMVK VILMEESNIQ PVRTPVTVCG    50
    DIHGQFYDLL ELFRVGGELP STNYIFMGDF VDRGYFSLET FTLFMLLKAR 100
    YPDKITLLRG NHESRQITQV YGFYDECQTK YGNANVWKYC CQVFDFLTLA 150
    AVIDNKILCV HGGLSPEVRT LDQIRILARA QEIPHEGSFC DLMWSDPEDI 200
    ESWTVSPRGA GWLFGSKVTT EFSQINDLTL IARAHQLVQE GYKYHFADKN 250
    LVTVWSAPNY CYRCGNVASV MKVDESLEPE FRIFSAVADE DRTVPPSRKR 300
    SEYFI 305
    Length:305
    Mass (Da):35,259
    Last modified:June 1, 1994 - v1
    Checksum:i001980A1CC7646D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13712 Genomic DNA. Translation: BAA02865.1.
    Z18925 Genomic DNA. Translation: CAA79358.1.
    CU329672 Genomic DNA. Translation: CAA20786.1.
    PIRiA47727.
    RefSeqiNP_588420.1. NM_001023411.2.

    Genome annotation databases

    EnsemblFungiiSPCC1739.12.1; SPCC1739.12.1:pep; SPCC1739.12.
    GeneIDi2538758.
    KEGGispo:SPCC1739.12.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13712 Genomic DNA. Translation: BAA02865.1 .
    Z18925 Genomic DNA. Translation: CAA79358.1 .
    CU329672 Genomic DNA. Translation: CAA20786.1 .
    PIRi A47727.
    RefSeqi NP_588420.1. NM_001023411.2.

    3D structure databases

    ProteinModelPortali P36614.
    SMRi P36614. Positions 3-284.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 275341. 32 interactions.
    IntActi P36614. 2 interactions.
    MINTi MINT-4689011.
    STRINGi 4896.SPCC1739.12-1.

    Proteomic databases

    MaxQBi P36614.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPCC1739.12.1 ; SPCC1739.12.1:pep ; SPCC1739.12 .
    GeneIDi 2538758.
    KEGGi spo:SPCC1739.12.

    Organism-specific databases

    PomBasei SPCC1739.12.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172696.
    KOi K15427.
    OMAi GELLCVH.
    OrthoDBi EOG7FFN29.
    PhylomeDBi P36614.

    Miscellaneous databases

    NextBioi 20799942.
    PROi P36614.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis."
      Shimanuki M., Kinoshita N., Ohkura H., Yoshida T., Toda T., Yanagida M.
      Mol. Biol. Cell 4:303-313(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 972 / HM123.
    2. "Interaction of the pim1/spi1 mitotic checkpoint with a protein phosphatase."
      Matsumoto T., Beach D.
      Mol. Biol. Cell 4:337-345(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    4. "The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore."
      Goshima G., Iwasaki O., Obuse C., Yanagida M.
      EMBO J. 22:2752-2763(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH STS5; ECK1 AND MIS12, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPPE1_SCHPO
    AccessioniPrimary (citable) accession number: P36614
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3