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P36614

- PPE1_SCHPO

UniProt

P36614 - PPE1_SCHPO

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Protein
Serine/threonine-protein phosphatase ppe1
Gene
ppe1, esp1, ppx1, SPCC1739.12
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has a role in chromosome segregation. May provide a dynamic connection between kinetochore microtubules and kinetochore chromatin. Negatively regulates mis12.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Manganese 1 By similarity
Metal bindingi53 – 531Manganese 1 By similarity
Metal bindingi79 – 791Manganese 1 By similarity
Metal bindingi79 – 791Manganese 2 By similarity
Metal bindingi111 – 1111Manganese 2 By similarity
Active sitei112 – 1121Proton donor By similarity
Metal bindingi161 – 1611Manganese 2 By similarity
Metal bindingi235 – 2351Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: PomBase
  3. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. mitotic nuclear division Source: UniProtKB-KW
  3. positive regulation of hydroxymethylglutaryl-CoA reductase (NADPH) activity Source: PomBase
  4. positive regulation of isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: PomBase
  5. positive regulation of protein dephosphorylation Source: PomBase
  6. regulation of cell shape Source: PomBase
  7. regulation of mitotic cell cycle Source: PomBase
  8. regulation of mitotic sister chromatid segregation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Mitosis

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase ppe1 (EC:3.1.3.16)
Alternative name(s):
Phosphatase esp1
Gene namesi
Name:ppe1
Synonyms:esp1, ppx1
ORF Names:SPCC1739.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome III

Organism-specific databases

PomBaseiSPCC1739.12.

Subcellular locationi

Nucleus
Note: Associated with chromatin.1 Publication

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. nuclear chromatin Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Serine/threonine-protein phosphatase ppe1
PRO_0000058882Add
BLAST

Proteomic databases

MaxQBiP36614.

Interactioni

Subunit structurei

Interacts with sts5, ekc1 and mis12.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ekc1O745112EBI-1153118,EBI-1153096

Protein-protein interaction databases

BioGridi275341. 32 interactions.
IntActiP36614. 2 interactions.
MINTiMINT-4689011.
STRINGi4896.SPCC1739.12-1.

Structurei

3D structure databases

ProteinModelPortaliP36614.
SMRiP36614. Positions 3-284.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
KOiK15427.
OMAiGELLCVH.
OrthoDBiEOG7FFN29.
PhylomeDBiP36614.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36614-1 [UniParc]FASTAAdd to Basket

« Hide

MFDLDEWIAT VRKCKYLPEH QLKRLCEMVK VILMEESNIQ PVRTPVTVCG    50
DIHGQFYDLL ELFRVGGELP STNYIFMGDF VDRGYFSLET FTLFMLLKAR 100
YPDKITLLRG NHESRQITQV YGFYDECQTK YGNANVWKYC CQVFDFLTLA 150
AVIDNKILCV HGGLSPEVRT LDQIRILARA QEIPHEGSFC DLMWSDPEDI 200
ESWTVSPRGA GWLFGSKVTT EFSQINDLTL IARAHQLVQE GYKYHFADKN 250
LVTVWSAPNY CYRCGNVASV MKVDESLEPE FRIFSAVADE DRTVPPSRKR 300
SEYFI 305
Length:305
Mass (Da):35,259
Last modified:June 1, 1994 - v1
Checksum:i001980A1CC7646D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13712 Genomic DNA. Translation: BAA02865.1.
Z18925 Genomic DNA. Translation: CAA79358.1.
CU329672 Genomic DNA. Translation: CAA20786.1.
PIRiA47727.
RefSeqiNP_588420.1. NM_001023411.2.

Genome annotation databases

EnsemblFungiiSPCC1739.12.1; SPCC1739.12.1:pep; SPCC1739.12.
GeneIDi2538758.
KEGGispo:SPCC1739.12.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13712 Genomic DNA. Translation: BAA02865.1 .
Z18925 Genomic DNA. Translation: CAA79358.1 .
CU329672 Genomic DNA. Translation: CAA20786.1 .
PIRi A47727.
RefSeqi NP_588420.1. NM_001023411.2.

3D structure databases

ProteinModelPortali P36614.
SMRi P36614. Positions 3-284.
ModBasei Search...

Protein-protein interaction databases

BioGridi 275341. 32 interactions.
IntActi P36614. 2 interactions.
MINTi MINT-4689011.
STRINGi 4896.SPCC1739.12-1.

Proteomic databases

MaxQBi P36614.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPCC1739.12.1 ; SPCC1739.12.1:pep ; SPCC1739.12 .
GeneIDi 2538758.
KEGGi spo:SPCC1739.12.

Organism-specific databases

PomBasei SPCC1739.12.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000172696.
KOi K15427.
OMAi GELLCVH.
OrthoDBi EOG7FFN29.
PhylomeDBi P36614.

Miscellaneous databases

NextBioi 20799942.
PROi P36614.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis."
    Shimanuki M., Kinoshita N., Ohkura H., Yoshida T., Toda T., Yanagida M.
    Mol. Biol. Cell 4:303-313(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / HM123.
  2. "Interaction of the pim1/spi1 mitotic checkpoint with a protein phosphatase."
    Matsumoto T., Beach D.
    Mol. Biol. Cell 4:337-345(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore."
    Goshima G., Iwasaki O., Obuse C., Yanagida M.
    EMBO J. 22:2752-2763(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH STS5; ECK1 AND MIS12, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPPE1_SCHPO
AccessioniPrimary (citable) accession number: P36614
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 3, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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