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Protein

DNA repair protein rad8

Gene

rad8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable helicase, member of the UBC2/RAD6 epistasis group. Functions with DNA repair protein rad18 in error-free postreplication DNA repair. Involved in the maintenance of wild-type rates of instability of simple repetitive sequences such as poly(GT) repeats (By similarity). Plays a role in surviving topoisomerase-mediated DNA damage (PubMed:14871939).By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi529 – 5368ATPPROSITE-ProRule annotation
Zinc fingeri877 – 92347RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • nucleotide-excision repair Source: PomBase
  • postreplication repair Source: PomBase
  • protein ubiquitination Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein rad81 Publication (EC:3.6.4.-)
Alternative name(s):
DNA repair protein RAD5 homolog1 Publication
Gene namesi
Name:rad81 Publication
ORF Names:SPAC13G6.01cImported, SPAC5H10.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC13G6.01c.
PomBaseiSPAC13G6.01c. rad8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11331133DNA repair protein rad8PRO_0000056127Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36607.
PRIDEiP36607.

PTM databases

iPTMnetiP36607.

Interactioni

Protein-protein interaction databases

BioGridi280467. 56 interactions.
MINTiMINT-4688972.

Structurei

3D structure databases

ProteinModelPortaliP36607.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini516 – 705190Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini971 – 1125155Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi656 – 6594DEGH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri877 – 92347RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000040492.
InParanoidiP36607.
KOiK15505.
OMAiMDPWWSP.
OrthoDBiEOG092C0L0F.
PhylomeDBiP36607.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014905. HIRAN.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF08797. HIRAN. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00910. HIRAN. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36607-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKVQKIID EAPLEENSPP RFFDSDVEAD SKPNDLTAAN SIVDLKTNSQ
60 70 80 90 100
HENANAAGKE YGDSGVSESW VLDFLSVTGE KTISEFLAQK IWKTSNGDLN
110 120 130 140 150
VAVDMYFDES FNIKNSNPDS ESQKDTDASL TQMDQLSNTV SVKDLSINRN
160 170 180 190 200
TNKKALNAVS PSLNLSSNSS VQDVSIDKEE MMKKQSRNAL TPLDFIMKKN
210 220 230 240 250
ELMKYIGCFG VEAYSTASGT RTLQAGERIY LERQKLSIKS QSRNSRKKSK
260 270 280 290 300
LLSINSSCYS NIVRFCNSDH HEIGKLPTEV ASVISTLMEQ GFWSFEAICI
310 320 330 340 350
YSDNIIRFGS NVTLQVYCFI NVNHPSLNRS PFTLATNSMQ EEEEHLKASF
360 370 380 390 400
AQNKRDHLLR LFTWIALEPD LEDCNTKESI HIDDILKTSS LPEARDESNS
410 420 430 440 450
DLTPSSTEDE EDVVSDQLAI LYDKVKTSGA ELPSAPKPST FALDLREYQK
460 470 480 490 500
QALYWMCCKE EGVQSDGSAP KLHPLWSRFR FPKDSEFPEF FKCSSDDDNT
510 520 530 540 550
HFYVNLYTGE TTMLFPNSMP YHRGGILADE MGLGKTIEVL SLIHSRPCFS
560 570 580 590 600
TDEIPEAFRH SKPSLPVASR TTLVVAPMSL LDQWHSEACK VSQGTKFRSM
610 620 630 640 650
IYYGSEKPLD LKSCVIDTST APLIIITSYG VLLSEFSQQS HSSGLFSVHW
660 670 680 690 700
FRVVLDEGHN IRNRESKTAK ACHSISSQNR WVITGTPIVN KLDDLYSLIK
710 720 730 740 750
FMRYEPWCNY TYWQTFVSLP YQSKDVLKAL NVVQSILEFL VLRRTKETKD
760 770 780 790 800
RNGNSIVTLP PKTVKIEYLD FSDSERKIYD SLYTKAKSTV NANIVAGTLF
810 820 830 840 850
RNYTTILGLL LRLRQACCDP VLLSNMTINS ETFDDFEFSV EQFNSLINQF
860 870 880 890 900
VVTGKPIPSD ILKIDTLKSF EALITECPIC CNEPIQNPLL LNCKHACCGD
910 920 930 940 950
CLSEHIQYQK RRNIIPPLCH TCRQPFNEQD VYKPFFVKNN GTQSTLLVGE
960 970 980 990 1000
EVKWKYWNRL QSVKLNGLLG QLRQLTHSSE PEKVVIFSQF TTFLDIIADV
1010 1020 1030 1040 1050
LESEKMGYAR FDGTMSQQMR STALETFRND PDVNVLIISL KAGGVGLNLT
1060 1070 1080 1090 1100
CANHVFIMDP WWSWSVEAQA IDRIHRLGQE KPVFVTRYIV RDTVEERMLK
1110 1120 1130
IQERKNFITG TLGMSEGKQQ VQSIEDIKML FEY
Length:1,133
Mass (Da):128,610
Last modified:June 1, 1994 - v1
Checksum:i5A70C93D9933BF63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74615 Genomic DNA. Translation: CAA52686.1.
CU329670 Genomic DNA. Translation: CAA89964.1.
PIRiS41478.
RefSeqiXP_001713034.1. XM_001712982.2.

Genome annotation databases

EnsemblFungiiSPAC13G6.01c.1; SPAC13G6.01c.1:pep; SPAC13G6.01c.
GeneIDi3361391.
KEGGispo:SPAC13G6.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74615 Genomic DNA. Translation: CAA52686.1.
CU329670 Genomic DNA. Translation: CAA89964.1.
PIRiS41478.
RefSeqiXP_001713034.1. XM_001712982.2.

3D structure databases

ProteinModelPortaliP36607.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280467. 56 interactions.
MINTiMINT-4688972.

PTM databases

iPTMnetiP36607.

Proteomic databases

MaxQBiP36607.
PRIDEiP36607.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC13G6.01c.1; SPAC13G6.01c.1:pep; SPAC13G6.01c.
GeneIDi3361391.
KEGGispo:SPAC13G6.01c.

Organism-specific databases

EuPathDBiFungiDB:SPAC13G6.01c.
PomBaseiSPAC13G6.01c. rad8.

Phylogenomic databases

HOGENOMiHOG000040492.
InParanoidiP36607.
KOiK15505.
OMAiMDPWWSP.
OrthoDBiEOG092C0L0F.
PhylomeDBiP36607.

Miscellaneous databases

PROiP36607.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014905. HIRAN.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF08797. HIRAN. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00910. HIRAN. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAD5_SCHPO
AccessioniPrimary (citable) accession number: P36607
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.