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Protein

Endoplasmic reticulum chaperone BiP

Gene

bip1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.By similarity

Enzyme regulationi

The chaperone activity is regulated by ATP-induced allosteric coupling of the nucleotide-binding (NBD) and substrate-binding (SBD) domains. In the ADP-bound and nucleotide-free (apo) states, the two domains have little interaction. In contrast, in the ATP-bound state the two domains are tightly coupled, which results in drastically accelerated kinetics in both binding and release of polypeptide substrates. J domain-containing co-chaperones stimulate the ATPase activity and are required for efficient substrate recognition by HSPA5/BiP.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 46ATPBy similarity4
Nucleotide bindingi232 – 234ATPBy similarity3
Nucleotide bindingi298 – 305ATPBy similarity8
Nucleotide bindingi369 – 372ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • endoplasmic reticulum unfolded protein response Source: PomBase
  • ubiquitin-dependent ERAD pathway Source: PomBase

Keywordsi

Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-3371453 Regulation of HSF1-mediated heat shock response

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum chaperone BiPCurated
Alternative name(s):
Immunoglobulin heavy chain-binding protein homologCurated
Short name:
BiPCurated
Gene namesi
Name:bip1
Synonyms:bip
ORF Names:SPAC22A12.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC22A12.15c
PomBaseiSPAC22A12.15c bip1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001358525 – 663Endoplasmic reticulum chaperone BiPAdd BLAST639

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Partially N-glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP36604
PaxDbiP36604
PRIDEiP36604

PTM databases

iPTMnetiP36604

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi278306, 4 interactors
ELMiP36604
IntActiP36604, 2 interactors
STRINGi4896.SPAC22A12.15c.1

Structurei

3D structure databases

ProteinModelPortaliP36604
SMRiP36604
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 285Nucleotide-binding (NBD)By similarityAdd BLAST155
Regioni404 – 504Substrate-binding (SBD)By similarityAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi660 – 663Prevents secretion from ER4

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000228135
InParanoidiP36604
KOiK09490
OMAiCVGVMQK
OrthoDBiEOG092C1VPN
PhylomeDBiP36604

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKFQLFSIL SYFVALFLLP MAFASGDDNS TESYGTVIGI DLGTTYSCVA
60 70 80 90 100
VMKNGRVEII ANDQGNRITP SYVAFTEDER LVGEAAKNQA PSNPENTIFD
110 120 130 140 150
IKRLIGRKFD EKTMAKDIKS FPFHIVNDKN RPLVEVNVGG KKKKFTPEEI
160 170 180 190 200
SAMILSKMKQ TAEAYLGKPV THAVVTVPAY FNDAQRQATK DAGTIAGLNV
210 220 230 240 250
IRIVNEPTAA AIAYGLDKTD TEKHIVVYDL GGGTFDVSLL SIDNGVFEVL
260 270 280 290 300
ATSGDTHLGG EDFDNRVINY LARTYNRKNN VDVTKDLKAM GKLKREVEKA
310 320 330 340 350
KRTLSSQKSV RIEIESFFNG QDFSETLSRA KFEEINMDLF KKTLKPVEQV
360 370 380 390 400
LKDSNLKKSE IDDIVLVGGS TRIPKVQELL ESFFGKKASK GINPDEAVAY
410 420 430 440 450
GAAVQAGVLS GEEGSDNIVL LDVIPLTLGI ETTGGVMTKL IGRNTPIPTR
460 470 480 490 500
KSQIFSTAVD NQNTVLIQVY EGERTLTKDN NLLGKFDLRG IPPAPRGVPQ
510 520 530 540 550
IEVTFEVDAN GVLTVSAVDK SGKGKPEKLV IKNDKGRLSE EDIERMVKEA
560 570 580 590 600
EEFAEEDKIL KERIEARNTL ENYAYSLKGQ FDDDEQLGGK VDPEDKQAVL
610 620 630 640 650
DAVEDVAEWL EIHGEDASKE EFEDQRQKLD AVVHPITQKL YSEGAGDADE
660
EDDDYFDDEA DEL
Length:663
Mass (Da):73,227
Last modified:December 15, 1998 - v2
Checksum:i9DB80112E6C35742
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173A → S in CAA45762 (PubMed:1373379).Curated1
Sequence conflicti301 – 302KR → NG in CAA45762 (PubMed:1373379).Curated2
Sequence conflicti336 – 345NMDLFKKTLK → KHGSLQEDFE in CAA45762 (PubMed:1373379).Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64416 Genomic DNA Translation: CAA45762.1
CU329670 Genomic DNA Translation: CAB16585.1
PIRiS20877
T38155
RefSeqiNP_593245.1, NM_001018642.2

Genome annotation databases

EnsemblFungiiSPAC22A12.15c.1; SPAC22A12.15c.1:pep; SPAC22A12.15c
GeneIDi2541815
KEGGispo:SPAC22A12.15c

Similar proteinsi

Entry informationi

Entry nameiBIP_SCHPO
AccessioniPrimary (citable) accession number: P36604
Secondary accession number(s): O13906
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 15, 1998
Last modified: June 20, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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