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Protein

Ribonucleoside-diphosphate reductase small chain

Gene

suc22

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi135 – 1351Iron 1PROSITE-ProRule annotation
Metal bindingi166 – 1661Iron 1PROSITE-ProRule annotation
Metal bindingi166 – 1661Iron 2By similarity
Metal bindingi169 – 1691Iron 1PROSITE-ProRule annotation
Active sitei173 – 1731PROSITE-ProRule annotation
Metal bindingi229 – 2291Iron 2By similarity
Metal bindingi263 – 2631Iron 2By similarity
Metal bindingi266 – 2661Iron 2By similarity

GO - Molecular functioni

  1. CDP reductase activity Source: PomBase
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. CDP metabolic process Source: PomBase
  2. dCDP biosynthetic process Source: PomBase
  3. DNA replication Source: UniProtKB-UniPathway
  4. purine deoxyribonucleoside triphosphate biosynthetic process Source: PomBase
  5. purine nucleoside metabolic process Source: PomBase
  6. pyrimidine deoxyribonucleoside triphosphate biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_306641. E2F mediated regulation of DNA replication.
REACT_343222. G1/S-Specific Transcription.
REACT_352639. Synthesis and interconversion of nucleotide di- and triphosphates.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:suc22
ORF Names:SPBC25D12.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

PomBaseiSPBC25D12.04.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication
Note: Localization to the cytoplasm is CSN- and checkpoint-dependent and is required for an efficient DNA damage response.

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
  4. ribonucleoside-diphosphate reductase complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391Ribonucleoside-diphosphate reductase small chainPRO_0000190463Add
BLAST

Proteomic databases

MaxQBiP36603.
PaxDbiP36603.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Protein-protein interaction databases

BioGridi276910. 4 interactions.
MINTiMINT-4688905.
STRINGi4896.SPBC25D12.04-1.

Structurei

3D structure databases

ProteinModelPortaliP36603.
SMRiP36603. Positions 60-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000255975.
InParanoidiP36603.
KOiK10808.
OMAiCNRRCQQ.
OrthoDBiEOG7T1RMH.
PhylomeDBiP36603.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36603-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLEHLEEFS YPKEHGEEVE YDSEQGVRKI YVKSIKETFN FDNVSEEEKQ
60 70 80 90 100
EGGDYYLGKK EDELDEVVLR PNPHRFVLFP IKYHEIWQFY KKAEASFWTA
110 120 130 140 150
EEIDLSKDLV DWDNKLNADE RYFISTVLAY FAASDGIVNE NLLERFSSEV
160 170 180 190 200
QIPEARCVYG FQIMIENIHS ETYSLLLDTY IREPKEKQRH FDAILTMGSI
210 220 230 240 250
KAKAKWALRW INDEDSTYAI RLVAFAAVEG IFFSGSFASI FWLKKRGLMP
260 270 280 290 300
GLTFSNELIC RDEGLHTDFA CLMFSHLKHR PGRKVVEAII VEAVDIEKEY
310 320 330 340 350
FTDALPVSLL GMNKDLMCQY IEFVADRLLV ALGNDKYYNV TNPFDFMENI
360 370 380 390
SLAGKTNFFE KKVSDYQIAG VMSGTKRAEK DDHTFTIDED F
Length:391
Mass (Da):45,405
Last modified:November 30, 2000 - v2
Checksum:i740ECDBECC8D0170
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti285 – 2884VVEA → LLKP in CAA46231 (PubMed:8479429).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65115 Genomic DNA. Translation: CAA46231.1.
CU329671 Genomic DNA. Translation: CAA20100.1.
PIRiS34808.
T39992.
RefSeqiNP_596546.1. NM_001022467.2.

Genome annotation databases

EnsemblFungiiSPBC25D12.04.1; SPBC25D12.04.1:pep; SPBC25D12.04.
GeneIDi2540381.
KEGGispo:SPBC25D12.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65115 Genomic DNA. Translation: CAA46231.1.
CU329671 Genomic DNA. Translation: CAA20100.1.
PIRiS34808.
T39992.
RefSeqiNP_596546.1. NM_001022467.2.

3D structure databases

ProteinModelPortaliP36603.
SMRiP36603. Positions 60-349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276910. 4 interactions.
MINTiMINT-4688905.
STRINGi4896.SPBC25D12.04-1.

Proteomic databases

MaxQBiP36603.
PaxDbiP36603.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC25D12.04.1; SPBC25D12.04.1:pep; SPBC25D12.04.
GeneIDi2540381.
KEGGispo:SPBC25D12.04.

Organism-specific databases

PomBaseiSPBC25D12.04.

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000255975.
InParanoidiP36603.
KOiK10808.
OMAiCNRRCQQ.
OrthoDBiEOG7T1RMH.
PhylomeDBiP36603.

Enzyme and pathway databases

UniPathwayiUPA00326.
ReactomeiREACT_306641. E2F mediated regulation of DNA replication.
REACT_343222. G1/S-Specific Transcription.
REACT_352639. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

NextBioi20801509.
PROiP36603.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The cell cycle genes cdc22+ and suc22+ of the fission yeast Schizosaccharomyces pombe encode the large and small subunits of ribonucleotide reductase."
    Fernandez-Sarabia M.J., McInerny C., Harris P., Gordon C., Fantes P.
    Mol. Gen. Genet. 238:241-251(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Cop9/signalosome subunits and Pcu4 regulate ribonucleotide reductase by both checkpoint-dependent and -independent mechanisms."
    Liu C., Powell K.A., Mundt K., Wu L., Carr A.M., Caspari T.
    Genes Dev. 17:1130-1140(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRIR2_SCHPO
AccessioniPrimary (citable) accession number: P36603
Secondary accession number(s): O74355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 1994
Last sequence update: November 30, 2000
Last modified: March 31, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.