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P36603

- RIR2_SCHPO

UniProt

P36603 - RIR2_SCHPO

Protein

Ribonucleoside-diphosphate reductase small chain

Gene

suc22

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi135 – 1351Iron 1PROSITE-ProRule annotation
    Metal bindingi166 – 1661Iron 1PROSITE-ProRule annotation
    Metal bindingi166 – 1661Iron 2By similarity
    Metal bindingi169 – 1691Iron 1PROSITE-ProRule annotation
    Active sitei173 – 1731PROSITE-ProRule annotation
    Metal bindingi229 – 2291Iron 2By similarity
    Metal bindingi263 – 2631Iron 2By similarity
    Metal bindingi266 – 2661Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: PomBase
    3. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway
    3. purine deoxyribonucleoside triphosphate biosynthetic process Source: PomBase
    4. purine nucleoside metabolic process Source: PomBase
    5. pyrimidine deoxyribonucleoside triphosphate biosynthetic process Source: PomBase
    6. pyrimidine nucleoside metabolic process Source: PomBase
    7. regulation of nuclear cell cycle DNA replication Source: PomBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_188629. G1/S-Specific Transcription.
    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Gene namesi
    Name:suc22
    ORF Names:SPBC25D12.04
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC25D12.04.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication
    Note: Localization to the cytoplasm is CSN- and checkpoint-dependent and is required for an efficient DNA damage response.

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. nucleus Source: PomBase
    4. ribonucleoside-diphosphate reductase complex Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391Ribonucleoside-diphosphate reductase small chainPRO_0000190463Add
    BLAST

    Proteomic databases

    MaxQBiP36603.
    PaxDbiP36603.

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small subunit.

    Protein-protein interaction databases

    BioGridi276910. 3 interactions.
    MINTiMINT-4688905.
    STRINGi4896.SPBC25D12.04-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP36603.
    SMRiP36603. Positions 60-349.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0208.
    HOGENOMiHOG000255975.
    KOiK10808.
    OMAiDWAINWI.
    OrthoDBiEOG7T1RMH.
    PhylomeDBiP36603.

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36603-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLEHLEEFS YPKEHGEEVE YDSEQGVRKI YVKSIKETFN FDNVSEEEKQ    50
    EGGDYYLGKK EDELDEVVLR PNPHRFVLFP IKYHEIWQFY KKAEASFWTA 100
    EEIDLSKDLV DWDNKLNADE RYFISTVLAY FAASDGIVNE NLLERFSSEV 150
    QIPEARCVYG FQIMIENIHS ETYSLLLDTY IREPKEKQRH FDAILTMGSI 200
    KAKAKWALRW INDEDSTYAI RLVAFAAVEG IFFSGSFASI FWLKKRGLMP 250
    GLTFSNELIC RDEGLHTDFA CLMFSHLKHR PGRKVVEAII VEAVDIEKEY 300
    FTDALPVSLL GMNKDLMCQY IEFVADRLLV ALGNDKYYNV TNPFDFMENI 350
    SLAGKTNFFE KKVSDYQIAG VMSGTKRAEK DDHTFTIDED F 391
    Length:391
    Mass (Da):45,405
    Last modified:December 1, 2000 - v2
    Checksum:i740ECDBECC8D0170
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti285 – 2884VVEA → LLKP in CAA46231. (PubMed:8479429)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65115 Genomic DNA. Translation: CAA46231.1.
    CU329671 Genomic DNA. Translation: CAA20100.1.
    PIRiS34808.
    T39992.
    RefSeqiNP_596546.1. NM_001022467.2.

    Genome annotation databases

    EnsemblFungiiSPBC25D12.04.1; SPBC25D12.04.1:pep; SPBC25D12.04.
    GeneIDi2540381.
    KEGGispo:SPBC25D12.04.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65115 Genomic DNA. Translation: CAA46231.1 .
    CU329671 Genomic DNA. Translation: CAA20100.1 .
    PIRi S34808.
    T39992.
    RefSeqi NP_596546.1. NM_001022467.2.

    3D structure databases

    ProteinModelPortali P36603.
    SMRi P36603. Positions 60-349.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276910. 3 interactions.
    MINTi MINT-4688905.
    STRINGi 4896.SPBC25D12.04-1.

    Proteomic databases

    MaxQBi P36603.
    PaxDbi P36603.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC25D12.04.1 ; SPBC25D12.04.1:pep ; SPBC25D12.04 .
    GeneIDi 2540381.
    KEGGi spo:SPBC25D12.04.

    Organism-specific databases

    PomBasei SPBC25D12.04.

    Phylogenomic databases

    eggNOGi COG0208.
    HOGENOMi HOG000255975.
    KOi K10808.
    OMAi DWAINWI.
    OrthoDBi EOG7T1RMH.
    PhylomeDBi P36603.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .
    Reactomei REACT_188629. G1/S-Specific Transcription.

    Miscellaneous databases

    NextBioi 20801509.
    PROi P36603.

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cell cycle genes cdc22+ and suc22+ of the fission yeast Schizosaccharomyces pombe encode the large and small subunits of ribonucleotide reductase."
      Fernandez-Sarabia M.J., McInerny C., Harris P., Gordon C., Fantes P.
      Mol. Gen. Genet. 238:241-251(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Cop9/signalosome subunits and Pcu4 regulate ribonucleotide reductase by both checkpoint-dependent and -independent mechanisms."
      Liu C., Powell K.A., Mundt K., Wu L., Carr A.M., Caspari T.
      Genes Dev. 17:1130-1140(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiRIR2_SCHPO
    AccessioniPrimary (citable) accession number: P36603
    Secondary accession number(s): O74355
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3