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P36603

- RIR2_SCHPO

UniProt

P36603 - RIR2_SCHPO

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Protein

Ribonucleoside-diphosphate reductase small chain

Gene

suc22

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi135 – 1351Iron 1PROSITE-ProRule annotation
Metal bindingi166 – 1661Iron 1PROSITE-ProRule annotation
Metal bindingi166 – 1661Iron 2By similarity
Metal bindingi169 – 1691Iron 1PROSITE-ProRule annotation
Active sitei173 – 1731PROSITE-ProRule annotation
Metal bindingi229 – 2291Iron 2By similarity
Metal bindingi263 – 2631Iron 2By similarity
Metal bindingi266 – 2661Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
  3. purine deoxyribonucleoside triphosphate biosynthetic process Source: PomBase
  4. purine nucleoside metabolic process Source: PomBase
  5. pyrimidine deoxyribonucleoside triphosphate biosynthetic process Source: PomBase
  6. pyrimidine nucleoside metabolic process Source: PomBase
  7. regulation of nuclear cell cycle DNA replication Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_188629. G1/S-Specific Transcription.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:suc22
ORF Names:SPBC25D12.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC25D12.04.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication
Note: Localization to the cytoplasm is CSN- and checkpoint-dependent and is required for an efficient DNA damage response.

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
  4. ribonucleoside-diphosphate reductase complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391Ribonucleoside-diphosphate reductase small chainPRO_0000190463Add
BLAST

Proteomic databases

MaxQBiP36603.
PaxDbiP36603.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Protein-protein interaction databases

BioGridi276910. 3 interactions.
MINTiMINT-4688905.
STRINGi4896.SPBC25D12.04-1.

Structurei

3D structure databases

ProteinModelPortaliP36603.
SMRiP36603. Positions 60-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000255975.
InParanoidiP36603.
KOiK10808.
OMAiDWAINWI.
OrthoDBiEOG7T1RMH.
PhylomeDBiP36603.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36603 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLEHLEEFS YPKEHGEEVE YDSEQGVRKI YVKSIKETFN FDNVSEEEKQ
60 70 80 90 100
EGGDYYLGKK EDELDEVVLR PNPHRFVLFP IKYHEIWQFY KKAEASFWTA
110 120 130 140 150
EEIDLSKDLV DWDNKLNADE RYFISTVLAY FAASDGIVNE NLLERFSSEV
160 170 180 190 200
QIPEARCVYG FQIMIENIHS ETYSLLLDTY IREPKEKQRH FDAILTMGSI
210 220 230 240 250
KAKAKWALRW INDEDSTYAI RLVAFAAVEG IFFSGSFASI FWLKKRGLMP
260 270 280 290 300
GLTFSNELIC RDEGLHTDFA CLMFSHLKHR PGRKVVEAII VEAVDIEKEY
310 320 330 340 350
FTDALPVSLL GMNKDLMCQY IEFVADRLLV ALGNDKYYNV TNPFDFMENI
360 370 380 390
SLAGKTNFFE KKVSDYQIAG VMSGTKRAEK DDHTFTIDED F
Length:391
Mass (Da):45,405
Last modified:December 1, 2000 - v2
Checksum:i740ECDBECC8D0170
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti285 – 2884VVEA → LLKP in CAA46231. (PubMed:8479429)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65115 Genomic DNA. Translation: CAA46231.1.
CU329671 Genomic DNA. Translation: CAA20100.1.
PIRiS34808.
T39992.
RefSeqiNP_596546.1. NM_001022467.2.

Genome annotation databases

EnsemblFungiiSPBC25D12.04.1; SPBC25D12.04.1:pep; SPBC25D12.04.
GeneIDi2540381.
KEGGispo:SPBC25D12.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65115 Genomic DNA. Translation: CAA46231.1 .
CU329671 Genomic DNA. Translation: CAA20100.1 .
PIRi S34808.
T39992.
RefSeqi NP_596546.1. NM_001022467.2.

3D structure databases

ProteinModelPortali P36603.
SMRi P36603. Positions 60-349.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276910. 3 interactions.
MINTi MINT-4688905.
STRINGi 4896.SPBC25D12.04-1.

Proteomic databases

MaxQBi P36603.
PaxDbi P36603.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC25D12.04.1 ; SPBC25D12.04.1:pep ; SPBC25D12.04 .
GeneIDi 2540381.
KEGGi spo:SPBC25D12.04.

Organism-specific databases

PomBasei SPBC25D12.04.

Phylogenomic databases

eggNOGi COG0208.
HOGENOMi HOG000255975.
InParanoidi P36603.
KOi K10808.
OMAi DWAINWI.
OrthoDBi EOG7T1RMH.
PhylomeDBi P36603.

Enzyme and pathway databases

UniPathwayi UPA00326 .
Reactomei REACT_188629. G1/S-Specific Transcription.

Miscellaneous databases

NextBioi 20801509.
PROi P36603.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The cell cycle genes cdc22+ and suc22+ of the fission yeast Schizosaccharomyces pombe encode the large and small subunits of ribonucleotide reductase."
    Fernandez-Sarabia M.J., McInerny C., Harris P., Gordon C., Fantes P.
    Mol. Gen. Genet. 238:241-251(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Cop9/signalosome subunits and Pcu4 regulate ribonucleotide reductase by both checkpoint-dependent and -independent mechanisms."
    Liu C., Powell K.A., Mundt K., Wu L., Carr A.M., Caspari T.
    Genes Dev. 17:1130-1140(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRIR2_SCHPO
AccessioniPrimary (citable) accession number: P36603
Secondary accession number(s): O74355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3