Reviewed,
UniProtKB/Swiss-Prot P36602 (RIR1_SCHPO)
Last modified
February 9, 2010.
Version 86.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ribonucleoside-diphosphate reductase large chain EC=1.17.4.1 Alternative name(s): Ribonucleotide reductase | ||||
| Gene names |
| ||||
| Organism | Schizosaccharomyces pombe (Fission yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4896 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 811 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. |
| Catalytic activity | 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin. |
| Enzyme regulation | Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the large subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site By similarity. |
| Pathway | |
| Subunit structure | Heterodimer of a large and a small subunit. |
| Sequence similarities | Belongs to the ribonucleoside diphosphate reductase large chain family. Contains 1 ATP-cone domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 811 | 811 | Ribonucleoside-diphosphate reductase large chain | PRO_0000187201 | |||||||
Regions | |||||||||||
| Domain | 1 – 92 | 92 | ATP-cone | ||||||||
| Region | 11 – 17 | 7 | Allosteric activator binding By similarity | ||||||||
| Region | 217 – 218 | 2 | Substrate binding By similarity | ||||||||
| Region | 285 – 288 | 4 | Allosteric effector binding, determines substrate specificity By similarity | ||||||||
| Region | 427 – 431 | 5 | Substrate binding By similarity | ||||||||
| Region | 602 – 606 | 5 | Substrate binding By similarity | ||||||||
Sites | |||||||||||
| Active site | 427 | 1 | Proton acceptor By similarity | ||||||||
| Active site | 429 | 1 | Cysteine radical intermediate By similarity | ||||||||
| Active site | 431 | 1 | Proton acceptor By similarity | ||||||||
| Binding site | 5 | 1 | Allosteric activator By similarity | ||||||||
| Binding site | 53 | 1 | Allosteric activator By similarity | ||||||||
| Binding site | 88 | 1 | Allosteric activator By similarity | ||||||||
| Binding site | 202 | 1 | Substrate By similarity | ||||||||
| Binding site | 247 | 1 | Substrate; via amide nitrogen By similarity | ||||||||
| Site | 218 | 1 | Important for hydrogen atom transfer By similarity | ||||||||
| Site | 226 | 1 | Allosteric effector binding, determines substrate specificity By similarity | ||||||||
| Site | 256 | 1 | Allosteric effector binding, determines substrate specificity By similarity | ||||||||
| Site | 444 | 1 | Important for hydrogen atom transfer By similarity | ||||||||
| Site | 736 | 1 | Important for electron transfer By similarity | ||||||||
| Site | 737 | 1 | Important for electron transfer By similarity | ||||||||
| Site | 806 | 1 | Interacts with thioredoxin/glutaredoxin By similarity | ||||||||
| Site | 809 | 1 | Interacts with thioredoxin/glutaredoxin By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 218 ↔ 444 | Redox-active By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 251 | 1 | N → Y in CAA46232. Ref.1 | ||||||||
| Sequence conflict | 327 | 1 | E → Q in CAA46232. Ref.1 | ||||||||
| Sequence conflict | 425 | 1 | C → S in CAA46232. Ref.1 | ||||||||
| Sequence conflict | 560 – 561 | 2 | AS → CI in CAA46232. Ref.1 | ||||||||
| Sequence conflict | 583 | 1 | L → F in CAA46232. Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The cell cycle genes cdc22+ and suc22+ of the fission yeast Schizosaccharomyces pombe encode the large and small subunits of ribonucleotide reductase." Fernandez-Sarabia M.J., McInerny C., Harris P., Gordon C., Fantes P. Mol. Gen. Genet. 238:241-251(1993) [PubMed: 8479429] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38366 / 972. |
| [2] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X65116 Genomic DNA. Translation: CAA46232.1. CU329670 Genomic DNA. Translation: CAA91952.1. |
| PIR | S34807. S62577. |
| RefSeq | NP_594491.1. |
3D structure databases | |
| SMR | P36602. Positions 15-752. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P36602. 1 interaction. |
| STRING | P36602. |
Genome annotation databases | |
| GeneID | 2541695. |
| GenomeReviews | Gene locus cdc22 in contig CU329670_GR. |
| KEGG | spo:SPAC1F7.05. |
| NMPDR | fig|4896.1.peg.4461. |
Organism-specific databases | |
| GeneDB_Spombe | SPAC1F7.05. |
Phylogenomic databases | |
| eggNOG | fuNOG04736. |
| HOGENOM | HBG296647. |
| OMA | GSLWSKG. |
| OrthoDB | EOG9JDJQ2. |
| PhylomeDB | P36602. |
Enzyme and pathway databases | |
| BRENDA | 1.17.4.1. 653. |
Gene expression databases | |
| ArrayExpress | P36602. |
Family and domain databases | |
| InterPro | IPR005144. ATP-cone. IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. IPR008926. Ribnucl_Rdtase_R1-su_N. [Graphical view] |
| PANTHER | PTHR11573. Ribncl_red_lg_C. 1 hit. |
| Pfam | PF03477. ATP-cone. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view] |
| PRINTS | PR01183. RIBORDTASEM1. |
| TIGRFAMs | TIGR02506. NrdE_NrdA. 1 hit. |
| PROSITE | PS51161. ATP_CONE. 1 hit. PS00089. RIBORED_LARGE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | RIR1_SCHPO | ||||||||
| Accession | Primary (citable) accession number: P36602 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


