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Protein

DNA repair protein rhp51

Gene

rhp51

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required both for recombination and for the repair of DNA damage caused by X-rays. Binds to single and double-stranded DNA, in the presence of magnesium, and exhibits DNA-dependent ATPase activity. Promotes DNA strand annealing and strand exchange via DNA recombinase activity and forms helical nucleoprotein filaments.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi149 – 156ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB
  • double-stranded DNA binding Source: UniProtKB
  • four-way junction DNA binding Source: GO_Central
  • identical protein binding Source: IntAct
  • protein-DNA loading ATPase activity Source: PomBase
  • recombinase activity Source: UniProtKB
  • single-stranded DNA binding Source: UniProtKB
  • Swi5-Sfr1 complex binding Source: PomBase

GO - Biological processi

  • chromatin silencing at centromere Source: PomBase
  • chromosome organization involved in meiotic cell cycle Source: GO_Central
  • DNA recombinase assembly Source: GO_Central
  • double-strand break repair involved in meiotic recombination Source: PomBase
  • double-strand break repair via homologous recombination Source: PomBase
  • mating type switching Source: PomBase
  • mitotic recombination Source: PomBase
  • mitotic recombination-dependent replication fork processing Source: PomBase
  • negative regulation of DNA strand resection involved in replication fork processing Source: PomBase
  • protein homooligomerization Source: UniProtKB
  • regulation of DNA recombination at centromere Source: PomBase
  • response to ionizing radiation Source: GO_Central
  • strand invasion Source: PomBase
  • telomere maintenance Source: PomBase

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein rhp51
Alternative name(s):
RAD51 homolog
Gene namesi
Name:rhp51
Synonyms:rad51
ORF Names:SPAC644.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

PomBaseiSPAC644.14c.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001229241 – 365DNA repair protein rhp51Add BLAST365

Proteomic databases

MaxQBiP36601.
PaxDbiP36601.
PRIDEiP36601.

Interactioni

Subunit structurei

Interacts with rad22, rad54, rdh54, rhp54, rti1, swi2 and swi5. Forms homooiligomers.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • Swi5-Sfr1 complex binding Source: PomBase

Protein-protein interaction databases

DIPiDIP-29235N.
IntActiP36601. 10 interactors.
MINTiP36601.
STRINGi4896.SPAC644.14c.1.

Structurei

3D structure databases

ProteinModelPortaliP36601.
SMRiP36601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000227426.
InParanoidiP36601.
KOiK04482.
OrthoDBiEOG092C3DRU.
PhylomeDBiP36601.

Family and domain databases

CDDicd01123. Rad51_DMC1_radA. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR033925. Rad51_DMC1_RadA.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
PfamiView protein in Pfam
PF08423. Rad51. 1 hit.
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiView protein in PROSITE
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P36601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTEVEMQV SAADTNNNEN GQAQSNYEYD VNVQDEEDEA AAGPMPLQML
60 70 80 90 100
EGNGITASDI KKIHEAGYYT VESIAYTPKR QLLLIKGISE AKADKLLGEA
110 120 130 140 150
SKLVPMGFTT ATEYHIRRSE LITITTGSKQ LDTLLQGGVE TGSITELFGE
160 170 180 190 200
FRTGKSQICH TLAVTCQLPI DMGGGEGKCL YIDTEGTFRP VRLLAVADRY
210 220 230 240 250
GLNGEEVLDN VAYARAYNAD HQLELLQQAA NMMSESRFSL LVVDSCTALY
260 270 280 290 300
RTDFSGRGEL SARQMHLARF MRTLQRLADE FGIAVVITNQ VVAQVDGISF
310 320 330 340 350
NPDPKKPIGG NILAHSSTTR LSLRKGRGEQ RICKIYDSPC LPESEAIFAI
360
NSDGVGDPKE IIAPV
Length:365
Mass (Da):39,823
Last modified:June 1, 1994 - v1
Checksum:i9F26EB9FA4F3C2BA
GO

Sequence cautioni

The sequence CAA80878 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15T → M (PubMed:8358431).Curated1
Sequence conflicti15T → M (PubMed:11859360).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13805 Genomic DNA. Translation: BAA02963.1.
Z22691 Genomic DNA. Translation: CAA80399.1.
Z24756 Genomic DNA. Translation: CAA80879.1.
Z24756 Genomic DNA. Translation: CAA80878.1. Different initiation.
CU329670 Genomic DNA. Translation: CAB90141.1.
PIRiS42107.
RefSeqiNP_593882.1. NM_001019312.2.

Genome annotation databases

GeneIDi2543580.
KEGGispo:SPAC644.14c.

Similar proteinsi

Entry informationi

Entry nameiRAD51_SCHPO
AccessioniPrimary (citable) accession number: P36601
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome