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Protein

DNA repair protein rhp51

Gene

rhp51

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required both for recombination and for the repair of DNA damage caused by X-rays. Binds to single and double-stranded DNA, in the presence of magnesium, and exhibits DNA-dependent ATPase activity. Promotes DNA strand annealing and strand exchange via DNA recombinase activity and forms helical nucleoprotein filaments.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi149 – 1568ATPSequence analysis

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB
  • double-stranded DNA binding Source: UniProtKB
  • double-stranded telomeric DNA binding Source: PomBase
  • four-way junction DNA binding Source: GO_Central
  • identical protein binding Source: IntAct
  • protein-DNA loading ATPase activity Source: PomBase
  • recombinase activity Source: UniProtKB
  • single-stranded DNA binding Source: UniProtKB
  • single-stranded telomeric DNA binding Source: PomBase

GO - Biological processi

  • chromosome organization involved in meiotic cell cycle Source: GO_Central
  • DNA recombinase assembly Source: GO_Central
  • double-strand break repair involved in meiotic recombination Source: PomBase
  • double-strand break repair via homologous recombination Source: PomBase
  • homologous recombination-dependent replication fork processing Source: PomBase
  • mating type switching Source: PomBase
  • meiotic DNA double-strand break formation Source: PomBase
  • mitotic recombination Source: PomBase
  • protein homooligomerization Source: UniProtKB
  • response to ionizing radiation Source: GO_Central
  • strand invasion Source: PomBase
  • telomere maintenance Source: PomBase
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein rhp51
Alternative name(s):
RAD51 homolog
Gene namesi
Name:rhp51
Synonyms:rad51
ORF Names:SPAC644.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

PomBaseiSPAC644.14c.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: PomBase
  • condensed nuclear chromosome Source: PomBase
  • linear element Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear envelope Source: PomBase
  • nucleoplasm Source: GO_Central
  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365DNA repair protein rhp51PRO_0000122924Add
BLAST

Proteomic databases

MaxQBiP36601.

Interactioni

Subunit structurei

Interacts with rad22, rad54, rdh54, rhp54, rti1, swi2 and swi5. Forms homooiligomers.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-926960,EBI-926960
rad22P365924EBI-926960,EBI-966242
rti1O429053EBI-926960,EBI-1167500
sfr1Q9USV12EBI-926960,EBI-927233
swi2Q106683EBI-926960,EBI-926914

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-29235N.
IntActiP36601. 9 interactions.
MINTiMINT-239321.

Structurei

3D structure databases

ProteinModelPortaliP36601.
SMRiP36601. Positions 45-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated
Contains 1 HhH domain.Curated

Phylogenomic databases

HOGENOMiHOG000227426.
InParanoidiP36601.
OrthoDBiEOG7TN02X.
PhylomeDBiP36601.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTEVEMQV SAADTNNNEN GQAQSNYEYD VNVQDEEDEA AAGPMPLQML
60 70 80 90 100
EGNGITASDI KKIHEAGYYT VESIAYTPKR QLLLIKGISE AKADKLLGEA
110 120 130 140 150
SKLVPMGFTT ATEYHIRRSE LITITTGSKQ LDTLLQGGVE TGSITELFGE
160 170 180 190 200
FRTGKSQICH TLAVTCQLPI DMGGGEGKCL YIDTEGTFRP VRLLAVADRY
210 220 230 240 250
GLNGEEVLDN VAYARAYNAD HQLELLQQAA NMMSESRFSL LVVDSCTALY
260 270 280 290 300
RTDFSGRGEL SARQMHLARF MRTLQRLADE FGIAVVITNQ VVAQVDGISF
310 320 330 340 350
NPDPKKPIGG NILAHSSTTR LSLRKGRGEQ RICKIYDSPC LPESEAIFAI
360
NSDGVGDPKE IIAPV
Length:365
Mass (Da):39,823
Last modified:June 1, 1994 - v1
Checksum:i9F26EB9FA4F3C2BA
GO

Sequence cautioni

The sequence CAA80878.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151T → M (PubMed:8358431).Curated
Sequence conflicti15 – 151T → M (PubMed:11859360).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13805 Genomic DNA. Translation: BAA02963.1.
Z22691 Genomic DNA. Translation: CAA80399.1.
Z24756 Genomic DNA. Translation: CAA80879.1.
Z24756 Genomic DNA. Translation: CAA80878.1. Different initiation.
CU329670 Genomic DNA. Translation: CAB90141.1.
PIRiS42107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13805 Genomic DNA. Translation: BAA02963.1.
Z22691 Genomic DNA. Translation: CAA80399.1.
Z24756 Genomic DNA. Translation: CAA80879.1.
Z24756 Genomic DNA. Translation: CAA80878.1. Different initiation.
CU329670 Genomic DNA. Translation: CAB90141.1.
PIRiS42107.

3D structure databases

ProteinModelPortaliP36601.
SMRiP36601. Positions 45-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29235N.
IntActiP36601. 9 interactions.
MINTiMINT-239321.

Proteomic databases

MaxQBiP36601.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

PomBaseiSPAC644.14c.

Phylogenomic databases

HOGENOMiHOG000227426.
InParanoidiP36601.
OrthoDBiEOG7TN02X.
PhylomeDBiP36601.

Enzyme and pathway databases

ReactomeiR-SPO-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.

Miscellaneous databases

PROiP36601.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA."
    Shinohara A., Ogawa H., Matsuda Y., Ushio N., Ikeo K., Ogawa T.
    Nat. Genet. 4:239-243(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning and sequence analysis of rhp51+, a Schizosaccharomyces pombe homolog of the Saccharomyces cerevisiae RAD51 gene."
    Jang Y.K., Jin Y.H., Kim E.M., Hong S.H., Fabre F., Park S.D.
    Gene 142:207-211(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  5. "Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks."
    Catlett M.G., Forsburg S.L.
    Mol. Biol. Cell 14:4707-4720(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: HOMODIMERIZATION, INTERACTION WITH RAD22; RAD54; RDH54; RHP54 AND RTI1.
  6. "Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast."
    Akamatsu Y., Dziadkowiec D., Ikeguchi M., Shinagawa H., Iwasaki H.
    Proc. Natl. Acad. Sci. U.S.A. 100:15770-15775(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SWI2 AND SWI5.
  7. "Fission yeast rad51 and dmc1, two efficient DNA recombinases forming helical nucleoprotein filaments."
    Sauvageau S., Stasiak A.Z., Banville I., Ploquin M., Stasiak A., Masson J.-Y.
    Mol. Cell. Biol. 25:4377-4387(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, HOMOOLIGOMERIZATION.

Entry informationi

Entry nameiRAD51_SCHPO
AccessioniPrimary (citable) accession number: P36601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.