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P36596

- FDFT_SCHPO

UniProt

P36596 - FDFT_SCHPO

Protein

Squalene synthase

Gene

erg9

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol.1 Publication

    Catalytic activityi

    2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    GO - Molecular functioni

    1. farnesyl-diphosphate farnesyltransferase activity Source: PomBase
    2. oxidoreductase activity Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. squalene synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. ergosterol biosynthetic process Source: PomBase
    2. isoprenoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase, Transferase

    Keywords - Biological processi

    Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    Magnesium, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00767; UER00751.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Squalene synthase (EC:2.5.1.21)
    Short name:
    SQS
    Short name:
    SS
    Alternative name(s):
    FPP:FPP farnesyltransferase
    Farnesyl-diphosphate farnesyltransferase
    Gene namesi
    Name:erg9
    ORF Names:SPBC646.05c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC646.05c.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum Source: PomBase
    2. endoplasmic reticulum membrane Source: PomBase
    3. integral component of membrane Source: UniProtKB-KW
    4. nuclear outer membrane-endoplasmic reticulum membrane network Source: PomBase

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 460460Squalene synthasePRO_0000067451Add
    BLAST

    Proteomic databases

    MaxQBiP36596.

    Interactioni

    Subunit structurei

    Monomer By similarity. Interacts with pof14.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    pof14Q102235EBI-1794119,EBI-1793014

    Protein-protein interaction databases

    BioGridi277625. 5 interactions.
    IntActiP36596. 2 interactions.
    MINTiMINT-3377067.
    STRINGi4896.SPBC646.05c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP36596.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei288 – 30821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei425 – 44521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phytoene/squalene synthase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1562.
    HOGENOMiHOG000186940.
    KOiK00801.
    OMAiEMRHAVC.
    OrthoDBiEOG7GJ6P5.
    PhylomeDBiP36596.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR002060. Squ/phyt_synthse.
    IPR006449. Squal_synth.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    TIGRFAMsiTIGR01559. squal_synth. 1 hit.
    PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36596-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLANRIEEI RCLCQYKLWN DLPSYGEDEN VPQNIRRCYQ LLDMTSRSFA    50
    VVIKELPNGI REAVMIFYLV LRGLDTVEDD MTLPLDKKLP ILRDFYKTIE 100
    VEGWTFNESG PNEKDRQLLV EFDVVIKEYL NLSEGYRNVI SNITKEMGDG 150
    MAYYASLAEK NDGFSVETIE DFNKYCHYVA GLVGIGLSRL FAQSKLEDPD 200
    LAHSQAISNS LGLFLQKVNI IRDYREDFDD NRHFWPREIW SKYTSSFGDL 250
    CLPDNSEKAL ECLSDMTANA LTHATDALVY LSQLKTQEIF NFCAIPQVMA 300
    IATLAAVFRN PDVFQTNVKI RKGQAVQIIL HSVNLKNVCD LFLRYTRDIH 350
    YKNTPKDPNF LKISIECGKI EQVSESLFPR RFREMYEKAY VSKLSEQKKG 400
    NGTQKAILND EQKELYRKDL QKLGISILFV FFIILVCLAV IFYVFNIRIH 450
    WSDFKELNLF 460
    Length:460
    Mass (Da):53,321
    Last modified:June 1, 1994 - v1
    Checksum:iBAEE7F24B6CE5D25
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06071 mRNA. Translation: AAA35343.1.
    CU329671 Genomic DNA. Translation: CAA22809.1.
    PIRiB48057.
    T40581.
    RefSeqiNP_595363.1. NM_001021271.2.

    Genome annotation databases

    EnsemblFungiiSPBC646.05c.1; SPBC646.05c.1:pep; SPBC646.05c.
    GeneIDi2541110.
    KEGGispo:SPBC646.05c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06071 mRNA. Translation: AAA35343.1 .
    CU329671 Genomic DNA. Translation: CAA22809.1 .
    PIRi B48057.
    T40581.
    RefSeqi NP_595363.1. NM_001021271.2.

    3D structure databases

    ProteinModelPortali P36596.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277625. 5 interactions.
    IntActi P36596. 2 interactions.
    MINTi MINT-3377067.
    STRINGi 4896.SPBC646.05c-1.

    Proteomic databases

    MaxQBi P36596.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC646.05c.1 ; SPBC646.05c.1:pep ; SPBC646.05c .
    GeneIDi 2541110.
    KEGGi spo:SPBC646.05c.

    Organism-specific databases

    PomBasei SPBC646.05c.

    Phylogenomic databases

    eggNOGi COG1562.
    HOGENOMi HOG000186940.
    KOi K00801.
    OMAi EMRHAVC.
    OrthoDBi EOG7GJ6P5.
    PhylomeDBi P36596.

    Enzyme and pathway databases

    UniPathwayi UPA00767 ; UER00751 .

    Miscellaneous databases

    NextBioi 20802223.
    PROi P36596.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR002060. Squ/phyt_synthse.
    IPR006449. Squal_synth.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    TIGRFAMsi TIGR01559. squal_synth. 1 hit.
    PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conservation between human and fungal squalene synthetases: similarities in structure, function, and regulation."
      Robinson G.W., Tsay Y.H., Kienzle B.K., Smith-Monroy C.A., Bishop R.W.
      Mol. Cell. Biol. 13:2706-2717(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF."
      Tafforeau L., Le Blastier S., Bamps S., Dewez M., Vandenhaute J., Hermand D.
      EMBO J. 25:4547-4556(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH POF14.
    4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFDFT_SCHPO
    AccessioniPrimary (citable) accession number: P36596
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3