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P36596

- FDFT_SCHPO

UniProt

P36596 - FDFT_SCHPO

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Protein

Squalene synthase

Gene

erg9

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol.1 Publication

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Mg2+By similarity

Pathwayi

GO - Molecular functioni

  1. farnesyl-diphosphate farnesyltransferase activity Source: PomBase
  2. oxidoreductase activity Source: UniProtKB-KW
  3. squalene synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. ergosterol biosynthetic process Source: PomBase
  2. isoprenoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Magnesium, NADP

Enzyme and pathway databases

ReactomeiREACT_258783. Cholesterol biosynthesis.
UniPathwayiUPA00767; UER00751.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:erg9
ORF Names:SPBC646.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC646.05c.

Subcellular locationi

Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei288 – 30821HelicalSequence AnalysisAdd
BLAST
Transmembranei425 – 44521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: PomBase
  2. endoplasmic reticulum membrane Source: PomBase
  3. integral component of membrane Source: UniProtKB-KW
  4. nuclear outer membrane-endoplasmic reticulum membrane network Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Squalene synthasePRO_0000067451Add
BLAST

Proteomic databases

MaxQBiP36596.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with pof14.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
pof14Q102235EBI-1794119,EBI-1793014

Protein-protein interaction databases

BioGridi277625. 4 interactions.
IntActiP36596. 2 interactions.
MINTiMINT-3377067.
STRINGi4896.SPBC646.05c-1.

Structurei

3D structure databases

ProteinModelPortaliP36596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000186940.
InParanoidiP36596.
KOiK00801.
OMAiEMRHAVC.
OrthoDBiEOG7GJ6P5.
PhylomeDBiP36596.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR01559. squal_synth. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36596-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLANRIEEI RCLCQYKLWN DLPSYGEDEN VPQNIRRCYQ LLDMTSRSFA
60 70 80 90 100
VVIKELPNGI REAVMIFYLV LRGLDTVEDD MTLPLDKKLP ILRDFYKTIE
110 120 130 140 150
VEGWTFNESG PNEKDRQLLV EFDVVIKEYL NLSEGYRNVI SNITKEMGDG
160 170 180 190 200
MAYYASLAEK NDGFSVETIE DFNKYCHYVA GLVGIGLSRL FAQSKLEDPD
210 220 230 240 250
LAHSQAISNS LGLFLQKVNI IRDYREDFDD NRHFWPREIW SKYTSSFGDL
260 270 280 290 300
CLPDNSEKAL ECLSDMTANA LTHATDALVY LSQLKTQEIF NFCAIPQVMA
310 320 330 340 350
IATLAAVFRN PDVFQTNVKI RKGQAVQIIL HSVNLKNVCD LFLRYTRDIH
360 370 380 390 400
YKNTPKDPNF LKISIECGKI EQVSESLFPR RFREMYEKAY VSKLSEQKKG
410 420 430 440 450
NGTQKAILND EQKELYRKDL QKLGISILFV FFIILVCLAV IFYVFNIRIH
460
WSDFKELNLF
Length:460
Mass (Da):53,321
Last modified:June 1, 1994 - v1
Checksum:iBAEE7F24B6CE5D25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06071 mRNA. Translation: AAA35343.1.
CU329671 Genomic DNA. Translation: CAA22809.1.
PIRiB48057.
T40581.
RefSeqiNP_595363.1. NM_001021271.2.

Genome annotation databases

EnsemblFungiiSPBC646.05c.1; SPBC646.05c.1:pep; SPBC646.05c.
GeneIDi2541110.
KEGGispo:SPBC646.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06071 mRNA. Translation: AAA35343.1 .
CU329671 Genomic DNA. Translation: CAA22809.1 .
PIRi B48057.
T40581.
RefSeqi NP_595363.1. NM_001021271.2.

3D structure databases

ProteinModelPortali P36596.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277625. 4 interactions.
IntActi P36596. 2 interactions.
MINTi MINT-3377067.
STRINGi 4896.SPBC646.05c-1.

Proteomic databases

MaxQBi P36596.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC646.05c.1 ; SPBC646.05c.1:pep ; SPBC646.05c .
GeneIDi 2541110.
KEGGi spo:SPBC646.05c.

Organism-specific databases

PomBasei SPBC646.05c.

Phylogenomic databases

eggNOGi COG1562.
HOGENOMi HOG000186940.
InParanoidi P36596.
KOi K00801.
OMAi EMRHAVC.
OrthoDBi EOG7GJ6P5.
PhylomeDBi P36596.

Enzyme and pathway databases

UniPathwayi UPA00767 ; UER00751 .
Reactomei REACT_258783. Cholesterol biosynthesis.

Miscellaneous databases

NextBioi 20802223.
PROi P36596.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF00494. SQS_PSY. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
TIGRFAMsi TIGR01559. squal_synth. 1 hit.
PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Conservation between human and fungal squalene synthetases: similarities in structure, function, and regulation."
    Robinson G.W., Tsay Y.H., Kienzle B.K., Smith-Monroy C.A., Bishop R.W.
    Mol. Cell. Biol. 13:2706-2717(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF."
    Tafforeau L., Le Blastier S., Bamps S., Dewez M., Vandenhaute J., Hermand D.
    EMBO J. 25:4547-4556(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH POF14.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFDFT_SCHPO
AccessioniPrimary (citable) accession number: P36596
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3