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Protein

Squalene synthase

Gene

erg9

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol.1 Publication

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Mg2+By similarity

Pathwayi: lanosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (erg9)
  2. Probable squalene monooxygenase (erg1)
  3. Lanosterol synthase (erg7)
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

GO - Molecular functioni

  • farnesyl-diphosphate farnesyltransferase activity Source: PomBase
  • squalene synthase activity Source: PomBase

GO - Biological processi

  • ergosterol biosynthetic process Source: PomBase
  • farnesyl diphosphate metabolic process Source: GO_Central
  • isoprenoid biosynthetic process Source: UniProtKB-KW

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processIsoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandMagnesium, NADP

Enzyme and pathway databases

ReactomeiR-SPO-191273 Cholesterol biosynthesis
UniPathwayiUPA00767; UER00751

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:erg9
ORF Names:SPBC646.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC646.05c
PomBaseiSPBC646.05c erg9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Transmembranei425 – 445HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000674511 – 460Squalene synthaseAdd BLAST460

Proteomic databases

MaxQBiP36596
PaxDbiP36596
PRIDEiP36596

PTM databases

iPTMnetiP36596

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with pof14.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
pof14Q102235EBI-1794119,EBI-1793014

Protein-protein interaction databases

BioGridi277625, 3 interactors
IntActiP36596, 2 interactors
MINTiP36596
STRINGi4896.SPBC646.05c.1

Structurei

3D structure databases

ProteinModelPortaliP36596
SMRiP36596
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000186940
InParanoidiP36596
KOiK00801
OMAiFNFVAIP
OrthoDBiEOG092C2IU6
PhylomeDBiP36596

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR002060 Squ/phyt_synthse
IPR006449 Squal_synth-like
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
TIGRFAMsiTIGR01559 squal_synth, 1 hit
PROSITEiView protein in PROSITE
PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

P36596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLANRIEEI RCLCQYKLWN DLPSYGEDEN VPQNIRRCYQ LLDMTSRSFA
60 70 80 90 100
VVIKELPNGI REAVMIFYLV LRGLDTVEDD MTLPLDKKLP ILRDFYKTIE
110 120 130 140 150
VEGWTFNESG PNEKDRQLLV EFDVVIKEYL NLSEGYRNVI SNITKEMGDG
160 170 180 190 200
MAYYASLAEK NDGFSVETIE DFNKYCHYVA GLVGIGLSRL FAQSKLEDPD
210 220 230 240 250
LAHSQAISNS LGLFLQKVNI IRDYREDFDD NRHFWPREIW SKYTSSFGDL
260 270 280 290 300
CLPDNSEKAL ECLSDMTANA LTHATDALVY LSQLKTQEIF NFCAIPQVMA
310 320 330 340 350
IATLAAVFRN PDVFQTNVKI RKGQAVQIIL HSVNLKNVCD LFLRYTRDIH
360 370 380 390 400
YKNTPKDPNF LKISIECGKI EQVSESLFPR RFREMYEKAY VSKLSEQKKG
410 420 430 440 450
NGTQKAILND EQKELYRKDL QKLGISILFV FFIILVCLAV IFYVFNIRIH
460
WSDFKELNLF
Length:460
Mass (Da):53,321
Last modified:June 1, 1994 - v1
Checksum:iBAEE7F24B6CE5D25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06071 mRNA Translation: AAA35343.1
CU329671 Genomic DNA Translation: CAA22809.1
PIRiB48057
T40581
RefSeqiNP_595363.1, NM_001021271.2

Genome annotation databases

EnsemblFungiiSPBC646.05c.1; SPBC646.05c.1:pep; SPBC646.05c
GeneIDi2541110
KEGGispo:SPBC646.05c

Similar proteinsi

Entry informationi

Entry nameiFDFT_SCHPO
AccessioniPrimary (citable) accession number: P36596
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 25, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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