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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC2

Gene

rpb6

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • DNA-templated transcription, elongation Source: PomBase
  • transcription from RNA polymerase III promoter Source: PomBase
  • transcription from RNA polymerase II promoter Source: PomBase
  • transcription from RNA polymerase I promoter Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiR-SPO-113418. Formation of the Early Elongation Complex.
R-SPO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-6781823. Formation of TC-NER Pre-Incision Complex.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-72086. mRNA Capping.
R-SPO-72165. mRNA Splicing - Minor Pathway.
R-SPO-73762. RNA Polymerase I Transcription Initiation.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Short name:
RNA polymerases I, II, and III subunit ABC2
Alternative name(s):
DNA-directed RNA polymerases I, II, and III 15 kDa polypeptide
RPC16
Gene namesi
Name:rpb6
Synonyms:rpo15
ORF Names:SPCC1020.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1020.04c.
PomBaseiSPCC1020.04c. rpb6.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • DNA-directed RNA polymerase I complex Source: PomBase
  • DNA-directed RNA polymerase II, core complex Source: PomBase
  • DNA-directed RNA polymerase III complex Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 142142DNA-directed RNA polymerases I, II, and III subunit RPABC2PRO_0000133796Add
BLAST

Post-translational modificationi

Phosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36595.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 14, 12 and 17 subunits, respectively.By similarity

Protein-protein interaction databases

BioGridi275655. 12 interactions.
IntActiP36595. 1 interaction.
MINTiMINT-1214385.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65F/R1-142[»]
ProteinModelPortaliP36595.
SMRiP36595. Positions 1-142.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36595.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000225272.
InParanoidiP36595.
KOiK03014.
OMAiPVMMAKY.
OrthoDBiEOG7MWH92.
PhylomeDBiP36595.

Family and domain databases

Gene3Di3.90.940.10. 1 hit.
HAMAPiMF_00192. RNApol_arch_K.
InterProiIPR020708. DNA-dir_RNA_polK_14-18kDa_CS.
IPR006110. Pol_omega/K/RPB6.
IPR012293. RNAP_RPB6_omega.
IPR028363. RPB6.
IPR006111. RpoK/Rpb6.
[Graphical view]
PANTHERiPTHR10773. PTHR10773. 1 hit.
PfamiPF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]
PIRSFiPIRSF500154. RPB6. 1 hit.
PIRSF000778. RpoK/RPB6. 1 hit.
SMARTiSM01409. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SUPFAMiSSF63562. SSF63562. 1 hit.
PROSITEiPS01111. RNA_POL_K_14KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYEEDEAF GMDGAVMEEE VDELEMIDEN GQSQQGVSHP GEPSTTVITE
60 70 80 90 100
DVASSKTAQS GKAVAKEDRT TTPYMTKYER ARILGTRALQ ISMNAPVLVD
110 120 130 140
LEGETDPLQI AMKELAQKKI PLLVRRYLPD GSYEDWSVAE LI
Length:142
Mass (Da):15,729
Last modified:June 1, 1994 - v1
Checksum:iFDC4E7AD5B495D79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00597 Genomic DNA. Translation: AAA52084.1.
L25592 Genomic DNA. Translation: AAB04116.1.
CU329672 Genomic DNA. Translation: CAA18992.1.
PIRiT40837.
RefSeqiNP_587956.1. NM_001022947.2.

Genome annotation databases

EnsemblFungiiSPCC1020.04c.1; SPCC1020.04c.1:pep; SPCC1020.04c.
GeneIDi2539083.
KEGGispo:SPCC1020.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00597 Genomic DNA. Translation: AAA52084.1.
L25592 Genomic DNA. Translation: AAB04116.1.
CU329672 Genomic DNA. Translation: CAA18992.1.
PIRiT40837.
RefSeqiNP_587956.1. NM_001022947.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65F/R1-142[»]
ProteinModelPortaliP36595.
SMRiP36595. Positions 1-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275655. 12 interactions.
IntActiP36595. 1 interaction.
MINTiMINT-1214385.

Proteomic databases

MaxQBiP36595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC1020.04c.1; SPCC1020.04c.1:pep; SPCC1020.04c.
GeneIDi2539083.
KEGGispo:SPCC1020.04c.

Organism-specific databases

EuPathDBiFungiDB:SPCC1020.04c.
PomBaseiSPCC1020.04c. rpb6.

Phylogenomic databases

HOGENOMiHOG000225272.
InParanoidiP36595.
KOiK03014.
OMAiPVMMAKY.
OrthoDBiEOG7MWH92.
PhylomeDBiP36595.

Enzyme and pathway databases

ReactomeiR-SPO-113418. Formation of the Early Elongation Complex.
R-SPO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-6781823. Formation of TC-NER Pre-Incision Complex.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-72086. mRNA Capping.
R-SPO-72165. mRNA Splicing - Minor Pathway.
R-SPO-73762. RNA Polymerase I Transcription Initiation.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

EvolutionaryTraceiP36595.
NextBioi20800255.
PROiP36595.

Family and domain databases

Gene3Di3.90.940.10. 1 hit.
HAMAPiMF_00192. RNApol_arch_K.
InterProiIPR020708. DNA-dir_RNA_polK_14-18kDa_CS.
IPR006110. Pol_omega/K/RPB6.
IPR012293. RNAP_RPB6_omega.
IPR028363. RPB6.
IPR006111. RpoK/Rpb6.
[Graphical view]
PANTHERiPTHR10773. PTHR10773. 1 hit.
PfamiPF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]
PIRSFiPIRSF500154. RPB6. 1 hit.
PIRSF000778. RpoK/RPB6. 1 hit.
SMARTiSM01409. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SUPFAMiSSF63562. SSF63562. 1 hit.
PROSITEiPS01111. RNA_POL_K_14KD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The fission yeast Schizosaccharomyces pombe rpb6 gene encodes the common phosphorylated subunit of RNA polymerase and complements a mutation in the corresponding gene of Saccharomyces cerevisiae."
    Shpakovski G.V.
    Gene 147:63-69(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Sequence of the rpo15 gene from S. pombe - a subunit common to all three RNA polymerases."
    Williams M.R., Konoha G., Yanagida M., Young R.F.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiRPAB2_SCHPO
AccessioniPrimary (citable) accession number: P36595
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.