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P36591

- DYR_SCHPO

UniProt

P36591 - DYR_SCHPO

Protein

Dihydrofolate reductase

Gene

dfr1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei239 – 2391NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei308 – 3081SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi246 – 2527NADPBy similarity
    Nucleotide bindingi292 – 2943NADPBy similarity
    Nucleotide bindingi314 – 3163NADPBy similarity
    Nucleotide bindingi365 – 3728NADPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. dihydrofolate reductase activity Source: PomBase
    3. NADP binding Source: InterPro

    GO - Biological processi

    1. dihydrofolate metabolic process Source: PomBase
    2. glycine biosynthetic process Source: InterPro
    3. nucleotide biosynthetic process Source: InterPro
    4. one-carbon metabolic process Source: UniProtKB-KW
    5. tetrahydrofolate biosynthetic process Source: PomBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, NADP, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_188358. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    REACT_188629. G1/S-Specific Transcription.
    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase (EC:1.5.1.3)
    Gene namesi
    Name:dfr1
    ORF Names:SPCC1223.08c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome III

    Organism-specific databases

    PomBaseiSPCC1223.08c.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 461461Dihydrofolate reductasePRO_0000186377Add
    BLAST

    Proteomic databases

    MaxQBiP36591.
    PaxDbiP36591.

    Interactioni

    Protein-protein interaction databases

    BioGridi275621. 8 interactions.
    MINTiMINT-4688829.
    STRINGi4896.SPCC1223.08c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP36591.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini233 – 447215DHFRPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni260 – 2656Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0262.
    KOiK00287.
    OMAiYSESENW.
    OrthoDBiEOG7Q8D07.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    3.40.50.1820. 2 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR005645. Serine_hydrolase_FSH.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    PF03959. FSH1. 1 hit.
    [Graphical view]
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53474. SSF53474. 2 hits.
    SSF53597. SSF53597. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36591-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKPLKVLCL HGWIQSGPVF SKKMGSVQKY LSKYAELHFP TGPVVADEEA    50
    DPNDEEEKKR LAALGGEQNG GKFGWFEVED FKNTYGSWDE SLECINQYMQ 100
    EKGPFDGLIG FSQGAGIGAM LAQMLQPGQP PNPYVQHPPF KFVVFVGGFR 150
    AEKPEFDHFY NPKLTTPSLH IAGTSDTLVP LARSKQLVER CENAHVLLHP 200
    GQHIVPQQAV YKTGIRDFMF SAPTKEPTKH PRDLTMIVAV SSPNLGIGKK 250
    NSMPWHIKQE MAYFANVTSS TESSGQLEEG KSKIMNVVIM GRSCYDSLPK 300
    KNRPLKDRIN IVITRNSNYN FGLTKKEKMP ENLYAADCID SALDLVAEKY 350
    GADSDIQVGK VFIIGGSFLY GSALYHPLTK NLLFTRIHKE YPCDSFFPFE 400
    PAESSDWVRK AHPELEKFVG IPVEEGRLKA ASSNKEEVEI EFELYGKNDD 450
    VNVALEKLSI C 461
    Length:461
    Mass (Da):51,523
    Last modified:May 30, 2000 - v2
    Checksum:iF6A3695F537A03C9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti126 – 1272QP → HA in AAA57051. (PubMed:8088538)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13703 mRNA. Translation: AAA57051.1.
    CU329672 Genomic DNA. Translation: CAA20877.1.
    PIRiT43248.
    RefSeqiNP_588353.1. NM_001023344.2.

    Genome annotation databases

    EnsemblFungiiSPCC1223.08c.1; SPCC1223.08c.1:pep; SPCC1223.08c.
    GeneIDi2539048.
    KEGGispo:SPCC1223.08c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13703 mRNA. Translation: AAA57051.1 .
    CU329672 Genomic DNA. Translation: CAA20877.1 .
    PIRi T43248.
    RefSeqi NP_588353.1. NM_001023344.2.

    3D structure databases

    ProteinModelPortali P36591.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 275621. 8 interactions.
    MINTi MINT-4688829.
    STRINGi 4896.SPCC1223.08c-1.

    Proteomic databases

    MaxQBi P36591.
    PaxDbi P36591.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPCC1223.08c.1 ; SPCC1223.08c.1:pep ; SPCC1223.08c .
    GeneIDi 2539048.
    KEGGi spo:SPCC1223.08c.

    Organism-specific databases

    PomBasei SPCC1223.08c.

    Phylogenomic databases

    eggNOGi COG0262.
    KOi K00287.
    OMAi YSESENW.
    OrthoDBi EOG7Q8D07.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .
    Reactomei REACT_188358. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    REACT_188629. G1/S-Specific Transcription.

    Miscellaneous databases

    NextBioi 20800222.

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    3.40.50.1820. 2 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR005645. Serine_hydrolase_FSH.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    PF03959. FSH1. 1 hit.
    [Graphical view ]
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53474. SSF53474. 2 hits.
    SSF53597. SSF53597. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The isolation and characterization of the gene (dfr1) encoding dihydrofolate reductase (DHFR) in Schizosaccharomyces pombe."
      Bertani L.E., Campbell J.L.
      Gene 147:131-135(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiDYR_SCHPO
    AccessioniPrimary (citable) accession number: P36591
    Secondary accession number(s): O74408
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3