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P36587

- NCPR_SCHPO

UniProt

P36587 - NCPR_SCHPO

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Protein
NADPH--cytochrome P450 reductase
Gene
ccr1, SPBC29A10.01, SPBC365.17
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

FAD.
FMN.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi153 – 18432FMN By similarity
Add
BLAST
Nucleotide bindingi306 – 31712FAD By similarity
Add
BLAST
Nucleotide bindingi437 – 44812FAD By similarity
Add
BLAST
Nucleotide bindingi535 – 55319NADP By similarity
Add
BLAST
Nucleotide bindingi630 – 64617NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. NADPH-hemoprotein reductase activity Source: PomBase
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. ergosterol biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
Gene namesi
Name:ccr1
ORF Names:SPBC29A10.01, SPBC365.17
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC29A10.01.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: PomBase
  2. endoplasmic reticulum membrane Source: PomBase
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 678678NADPH--cytochrome P450 reductase
PRO_0000167609Add
BLAST

Proteomic databases

MaxQBiP36587.

Interactioni

Protein-protein interaction databases

BioGridi277553. 27 interactions.
MINTiMINT-4688783.
STRINGi4896.SPBC29A10.01-1.

Structurei

3D structure databases

ProteinModelPortaliP36587.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 208156Flavodoxin-like
Add
BLAST
Domaini267 – 514248FAD-binding FR-type
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0369.
HOGENOMiHOG000282027.
KOiK00327.
OMAiLGKHILF.
OrthoDBiEOG744TKC.
PhylomeDBiP36587.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36587-1 [UniParc]FASTAAdd to Basket

« Hide

MKTYEYVLLV IILILSLCYF IYNNFLNKPK APERRVVATD SIVELMEAEK    50
LTAAVFFGSQ TGTAEDFAYR FSTEAKANFN LTNMVFDLEN YDLTDLDNFD 100
RSKLLVFFLA TYGEGEPTDN AEAFLQLLEG DDTVFSSGKG IEDTPFEGIR 150
YAIFGLGNHT YEYYNAMAKK VDAAMTRLGA TRVGNLGLGD DAAGMLEEDY 200
LQWKDDTLPE IGKLFHLQEV HKEYNPMFEV IEKPEISNTS STVFLGEPSR 250
QQLKGNVASK APRSQANPFF SSPVRSLELF KSGSRNCLHL ELDIADSGMR 300
YQTGDYASIC PMNPSQAVDD LLEVLGLKEK RDTVIIVKPI DTLDKAPVLS 350
PTTYDTVFRY YYEICGIVSR QLLSFIAPFA PTPESKQELE KLGNDYDYFK 400
KNVVDLHLNL AQVLRRVSPD APFTKLPFSM LLENMAHMKP RYYSISSSSV 450
VHPDKVHVTA VVDKKEWTDK NHIFYGLTTN YLLAHCRHMH GEKIPHPNGL 500
EYTLEGPRKN WTGKIPMFVK KSTFRLAPPD VPIIMVGPGT GVAPFRGFVM 550
ERANLASKGV KVAKTLLFYG CQYSDKDFLY KEEWQQYKDV LKDSFELITA 600
FSREQDHKIY VQHRLLEHSD TIAKLVEEGA AFYICGDADH MAKDVVNALA 650
SILTTVDVDG MKAVKALRDD NRFFEDTW 678
Length:678
Mass (Da):76,775
Last modified:June 1, 1994 - v1
Checksum:i6AEDDFBA6DE39C8F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64702 mRNA. Translation: CAA45956.1.
CU329671 Genomic DNA. Translation: CAB44769.1.
AB027780 Genomic DNA. Translation: BAA87084.1.
PIRiS29123.
T40056.
RefSeqiNP_596046.1. NM_001021956.2.

Genome annotation databases

EnsemblFungiiSPBC29A10.01.1; SPBC29A10.01.1:pep; SPBC29A10.01.
GeneIDi2541038.
KEGGispo:SPBC29A10.01.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64702 mRNA. Translation: CAA45956.1 .
CU329671 Genomic DNA. Translation: CAB44769.1 .
AB027780 Genomic DNA. Translation: BAA87084.1 .
PIRi S29123.
T40056.
RefSeqi NP_596046.1. NM_001021956.2.

3D structure databases

ProteinModelPortali P36587.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277553. 27 interactions.
MINTi MINT-4688783.
STRINGi 4896.SPBC29A10.01-1.

Proteomic databases

MaxQBi P36587.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC29A10.01.1 ; SPBC29A10.01.1:pep ; SPBC29A10.01 .
GeneIDi 2541038.
KEGGi spo:SPBC29A10.01.

Organism-specific databases

PomBasei SPBC29A10.01.

Phylogenomic databases

eggNOGi COG0369.
HOGENOMi HOG000282027.
KOi K00327.
OMAi LGKHILF.
OrthoDBi EOG744TKC.
PhylomeDBi P36587.

Miscellaneous databases

NextBioi 20802152.
PROi P36587.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000208. P450R. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structurally and functionally conserved regions of cytochrome P-450 reductase as targets for DNA amplification by the polymerase chain reaction. Cloning and nucleotide sequence of the Schizosaccharomyces pombe cDNA."
    Miles J.S.
    Biochem. J. 287:195-200(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 128-276.
    Strain: ATCC 38364 / 968.

Entry informationi

Entry nameiNCPR_SCHPO
AccessioniPrimary (citable) accession number: P36587
Secondary accession number(s): Q9USU6, Q9UU68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi