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P36587

- NCPR_SCHPO

UniProt

P36587 - NCPR_SCHPO

Protein

NADPH--cytochrome P450 reductase

Gene

ccr1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

    Catalytic activityi

    NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

    Cofactori

    FAD.
    FMN.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi153 – 18432FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi306 – 31712FADBy similarityAdd
    BLAST
    Nucleotide bindingi437 – 44812FADBy similarityAdd
    BLAST
    Nucleotide bindingi535 – 55319NADPBy similarityAdd
    BLAST
    Nucleotide bindingi630 – 64617NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. NADPH-hemoprotein reductase activity Source: PomBase

    GO - Biological processi

    1. ergosterol biosynthetic process Source: PomBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH--cytochrome P450 reductase (EC:1.6.2.4)
    Short name:
    CPR
    Short name:
    P450R
    Gene namesi
    Name:ccr1
    ORF Names:SPBC29A10.01, SPBC365.17
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC29A10.01.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: PomBase
    2. endoplasmic reticulum membrane Source: PomBase
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 678678NADPH--cytochrome P450 reductasePRO_0000167609Add
    BLAST

    Proteomic databases

    MaxQBiP36587.

    Interactioni

    Protein-protein interaction databases

    BioGridi277553. 27 interactions.
    MINTiMINT-4688783.
    STRINGi4896.SPBC29A10.01-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP36587.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini53 – 208156Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini267 – 514248FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0369.
    HOGENOMiHOG000282027.
    KOiK00327.
    OMAiLGKHILF.
    OrthoDBiEOG744TKC.
    PhylomeDBiP36587.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36587-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTYEYVLLV IILILSLCYF IYNNFLNKPK APERRVVATD SIVELMEAEK    50
    LTAAVFFGSQ TGTAEDFAYR FSTEAKANFN LTNMVFDLEN YDLTDLDNFD 100
    RSKLLVFFLA TYGEGEPTDN AEAFLQLLEG DDTVFSSGKG IEDTPFEGIR 150
    YAIFGLGNHT YEYYNAMAKK VDAAMTRLGA TRVGNLGLGD DAAGMLEEDY 200
    LQWKDDTLPE IGKLFHLQEV HKEYNPMFEV IEKPEISNTS STVFLGEPSR 250
    QQLKGNVASK APRSQANPFF SSPVRSLELF KSGSRNCLHL ELDIADSGMR 300
    YQTGDYASIC PMNPSQAVDD LLEVLGLKEK RDTVIIVKPI DTLDKAPVLS 350
    PTTYDTVFRY YYEICGIVSR QLLSFIAPFA PTPESKQELE KLGNDYDYFK 400
    KNVVDLHLNL AQVLRRVSPD APFTKLPFSM LLENMAHMKP RYYSISSSSV 450
    VHPDKVHVTA VVDKKEWTDK NHIFYGLTTN YLLAHCRHMH GEKIPHPNGL 500
    EYTLEGPRKN WTGKIPMFVK KSTFRLAPPD VPIIMVGPGT GVAPFRGFVM 550
    ERANLASKGV KVAKTLLFYG CQYSDKDFLY KEEWQQYKDV LKDSFELITA 600
    FSREQDHKIY VQHRLLEHSD TIAKLVEEGA AFYICGDADH MAKDVVNALA 650
    SILTTVDVDG MKAVKALRDD NRFFEDTW 678
    Length:678
    Mass (Da):76,775
    Last modified:June 1, 1994 - v1
    Checksum:i6AEDDFBA6DE39C8F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64702 mRNA. Translation: CAA45956.1.
    CU329671 Genomic DNA. Translation: CAB44769.1.
    AB027780 Genomic DNA. Translation: BAA87084.1.
    PIRiS29123.
    T40056.
    RefSeqiNP_596046.1. NM_001021956.2.

    Genome annotation databases

    EnsemblFungiiSPBC29A10.01.1; SPBC29A10.01.1:pep; SPBC29A10.01.
    GeneIDi2541038.
    KEGGispo:SPBC29A10.01.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64702 mRNA. Translation: CAA45956.1 .
    CU329671 Genomic DNA. Translation: CAB44769.1 .
    AB027780 Genomic DNA. Translation: BAA87084.1 .
    PIRi S29123.
    T40056.
    RefSeqi NP_596046.1. NM_001021956.2.

    3D structure databases

    ProteinModelPortali P36587.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277553. 27 interactions.
    MINTi MINT-4688783.
    STRINGi 4896.SPBC29A10.01-1.

    Proteomic databases

    MaxQBi P36587.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC29A10.01.1 ; SPBC29A10.01.1:pep ; SPBC29A10.01 .
    GeneIDi 2541038.
    KEGGi spo:SPBC29A10.01.

    Organism-specific databases

    PomBasei SPBC29A10.01.

    Phylogenomic databases

    eggNOGi COG0369.
    HOGENOMi HOG000282027.
    KOi K00327.
    OMAi LGKHILF.
    OrthoDBi EOG744TKC.
    PhylomeDBi P36587.

    Miscellaneous databases

    NextBioi 20802152.
    PROi P36587.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000208. P450R. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structurally and functionally conserved regions of cytochrome P-450 reductase as targets for DNA amplification by the polymerase chain reaction. Cloning and nucleotide sequence of the Schizosaccharomyces pombe cDNA."
      Miles J.S.
      Biochem. J. 287:195-200(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
      Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
      Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 128-276.
      Strain: ATCC 38364 / 968.

    Entry informationi

    Entry nameiNCPR_SCHPO
    AccessioniPrimary (citable) accession number: P36587
    Secondary accession number(s): Q9USU6, Q9UU68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3