Reviewed,
UniProtKB/Swiss-Prot P36587 (NCPR_SCHPO)
Last modified
November 3, 2009.
Version 83.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: NADPH--cytochrome P450 reductase Short name=CPR Short name=P450R EC=1.6.2.4 | ||||
| Gene names |
| ||||
| Organism | Schizosaccharomyces pombe (Fission yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4896 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 678 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. |
| Catalytic activity | NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein. |
| Cofactor | FAD. FMN. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane protein. |
| Sequence similarities | In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | FAD FMN Flavoprotein NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum Inferred from direct assay. Source: GeneDB_SPombe integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | FMN binding Inferred from electronic annotation. Source: InterPro NADPH-hemoprotein reductase activityTraceable author statement. Source: GeneDB_SPombe electron carrier activityInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 678 | 678 | NADPH--cytochrome P450 reductase | PRO_0000167609 | |||||
Regions | |||||||||
| Transmembrane | 6 – 26 | 21 | Potential | ||||||
| Domain | 53 – 208 | 156 | Flavodoxin-like | ||||||
| Domain | 267 – 514 | 248 | FAD-binding FR-type | ||||||
| Nucleotide binding | 153 – 184 | 32 | FMN By similarity | ||||||
| Nucleotide binding | 306 – 317 | 12 | FAD By similarity | ||||||
| Nucleotide binding | 437 – 448 | 12 | FAD By similarity | ||||||
| Nucleotide binding | 535 – 553 | 19 | NADP By similarity | ||||||
| Nucleotide binding | 630 – 646 | 17 | NADP By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Structurally and functionally conserved regions of cytochrome P-450 reductase as targets for DNA amplification by the polymerase chain reaction. Cloning and nucleotide sequence of the Schizosaccharomyces pombe cDNA." Miles J.S. Biochem. J. 287:195-200(1992) [PubMed: 1417773] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972. |
| [3] | "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 128-276. Strain: ATCC 38364 / 968. |
Cross-references
Sequence databases | |
|---|---|
| X64702 mRNA. Translation: CAA45956.1. CU329671 Genomic DNA. Translation: CAB44769.1. AB027780 Genomic DNA. Translation: BAA87084.1. | |
| PIR | S29123. T40056. |
| RefSeq | NP_596046.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1AMO based on UniProtKB P00388. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P36587. |
Genome annotation databases | |
| GeneID | 2541038. |
| GenomeReviews | Gene locus ccr1 in contig CU329671_GR. |
| KEGG | spo:SPBC29A10.01. |
| NMPDR | fig|4896.1.peg.1912. |
Organism-specific databases | |
| GeneDB_Spombe | SPBC29A10.01. |
Phylogenomic databases | |
| OMA | ATEIWNI. |
Enzyme and pathway databases | |
| BioCyc | SPOM-XXX-01:SPOM-XXX-01-004880-MON. |
| BRENDA | 1.6.2.4. 653. |
Gene expression databases | |
| ArrayExpress | P36587. |
Family and domain databases | |
| InterPro | IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR015702. NADPH_Cyt_P450_Rdtase. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view] |
| PANTHER | PTHR19384:SF17. NADPH_Cyt_Red. 1 hit. |
| Pfam | PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view] |
| PRINTS | PR00369. FLAVODOXIN. PR00371. FPNCR. |
| PROSITE | PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NCPR_SCHPO | ||||||||
| Accession | Primary (citable) accession number: P36587 Secondary accession number(s): Q9USU6, Q9UU68 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


