P36583 (PCK2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase C-like 2
EC=2.7.11.13

Gene names

Name:	pck2
Synonyms:	pkc1, sts6
ORF Names:	SPBC12D12.04c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1016 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the control of the cell shape. Target of the inhibitor staurosporine.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with rho2. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. Contains 2 REM (Hr1) repeats.

Ontologies

Keywords
Biological process	Cell shape
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of bipolar cell growth Inferred from mutant phenotype. Source: GeneDB_Spombe intracellular signal transduction Inferred by curator. Source: GeneDB_Spombe positive regulation of calcium-mediated signaling Inferred from mutant phenotype. Source: GeneDB_Spombe regulation of alpha-glucan biosynthetic process Inferred from mutant phenotype. Source: GeneDB_Spombe regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell wall (1->3)-beta-D-glucan biosynthetic process Inferred from mutant phenotype. Source: GeneDB_Spombe regulation of establishment or maintenance of cell polarity Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	cell division site Inferred from direct assay. Source: GeneDB_Spombe cell septum Inferred from direct assay. Source: GeneDB_Spombe cell tip Inferred from direct assay. Source: GeneDB_Spombe intracellular Inferred by curator. Source: GOC
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: GeneDB_Spombe protein kinase C activity Traceable author statement. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1016	1016	Protein kinase C-like 2		PRO_0000055741
Regions
	Repeat	6 – 79	74	REM 1
	Repeat	123 – 198	76	REM 2
	Domain	683 – 942	260	Protein kinase
	Domain	943 – 1013	71	AGC-kinase C-terminal
	Zinc finger	405 – 453	49	Phorbol-ester/DAG-type 1
	Zinc finger	473 – 523	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	689 – 697	9	ATP By similarity
	Compositional bias	554 – 560	7	Poly-His
Sites
	Active site	808	1	Proton acceptor By similarity
	Binding site	712	1	ATP By similarity
Amino acid modifications
	Modified residue	984	1	Phosphothreonine Ref.6
Experimental info
	Sequence conflict	152	1	E → V in BAA03268. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36583 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 060CDC4F718A0069	FASTA	1,016	116,005
	10 20 30 40 50 60 MDMIDEAITE VVRKIERERS VIHGALSMKR LTQNQTVHQQ LHSNIEESKK SIIYLEERLE 70 80 90 100 110 120 KLKLRKNGVR KSNSEKPSVG IEKNPSFSTT KSAKSFSSTS SNIDSNLDLL NYDTPLTISK 130 140 150 160 170 180 ISFLLQQLEF KLSVEEQYRK GIEKMAKLYE REHDRRSIAE AEKKYVESAQ KITLLKQALK 190 200 210 220 230 240 RYHDLHIEID EEDVPSTESR GNLNARRPQS GLLKITVGSL RNVTHSAGIS KQTEMIVAIR 250 260 270 280 290 300 AEDLERARTR PSRTDRFNET FEIDLEKTNE VEIVVYEKKN EKLLLPVGLL WIRLSDLVEK 310 320 330 340 350 360 QRRKKVEQEV SDKGWVSADK MINQRLSIFL PSALNNISKP ESTDRPNTAS GNQSVSAWFS 370 380 390 400 410 420 LEPMGQINLT MNFTKHNTRK RPMDAGLGRQ GAIRQRKESV HEVYGHKFLQ HQFYQIMRCA 430 440 450 460 470 480 LCGEFLKNAA GMQCIDCHYT CHKKCYPKVV TKCISKSSDS ASSEYEKINH RIPHHFESHT 490 500 510 520 530 540 NIGANWCCHC GYILPLGRKT ARKCTECGIT AHAQCVHLVP DFCGMSMEMA NRVISEIRTT 550 560 570 580 590 600 KIYKAQQHKQ KSSHHKHHHH KKSKSSSSKH KENDKASVSI TTTTTPSITP ADPVPTSPKP 610 620 630 640 650 660 LAIEPVKRKP VHAGNLEVTS VSDNKLGATV QVVEQKVDDK ADALTKPPSL DAVKEPIPVP 670 680 690 700 710 720 SVETSVVAQD LTHKAKRIGL EDFTFLSVLG KGNFGKVMLA ELKSEKQLYA IKVLKKEFIL 730 740 750 760 770 780 ENDEVESTKS EKRVFLVANR ERHPFLVNLH SCFQTETRIY FVMDFVSGGD LMLHIQQEQF 790 800 810 820 830 840 SRRRAQFYAA EVCLALKYFH DNGIIYRDLK LDNILLSPDG HVKVADYGLC KEDMWHDNTT 850 860 870 880 890 900 ATFCGTPEFM APEILLEQQY TRSVDWWAFG VLIYQMLLGQ SPFRGEDEEE IFDAILSDEP 910 920 930 940 950 960 LYPIHMPRDS VSILQQLLTR DPKKRLGSGP NDAEDVMTHP FFSNINWDDI YHKRTQPPYI 970 980 990 1000 1010 PSLNSPTDTK YFDEEFTREL PVLTPVNSIL TKEMQQHFEG FSYSCEDDKP STTDNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control." Toda T., Shimanuki M., Yanagida M. EMBO J. 12:1987-1995(1993) [PubMed: 8491190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A Ca(2+)-independent protein kinase C from fission yeast." Mazzei G.J., Schmid E.M., Knowles J.K., Payton M.A., Maundrell K.G. J. Biol. Chem. 268:7401-7406(1993) [PubMed: 8463273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[4]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 755-928. Strain: ATCC 38364 / 968.
[5]	"Schizosaccharomyces pombe rho2p GTPase regulates cell wall alpha-glucan biosynthesis through the protein kinase pck2p." Calonge T.M., Nakano K., Arellano M., Arai R., Katayama S., Toda T., Mabuchi I., Perez P. Mol. Biol. Cell 11:4393-4401(2000) [PubMed: 11102532] [Abstract] Cited for: INTERACTION WITH RHO2.
[6]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-984, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14338 Genomic DNA. Translation: BAA03268.1. L07637 Genomic DNA. Translation: AAA35323.1. CU329671 Genomic DNA. Translation: CAA22678.1. AB027824 Genomic DNA. Translation: BAA87128.1.
PIR	A46079.
RefSeq	NP_595950.1. NM_001021859.1.
3D structure databases
ProteinModelPortal	P36583.
SMR	P36583. Positions 680-1007.
ModBase	Search...
Protein-protein interaction databases
STRING	P36583.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC12D12.04c.1; SPBC12D12.04c.1:pep; SPBC12D12.04c.
GeneID	2539649.
GenomeReviews	Gene locus pck2 in contig CU329671_GR.
KEGG	spo:SPBC12D12.04c.
NMPDR	fig|4896.1.peg.1816.
Organism-specific databases
GeneDB_Spombe	SPBC12D12.04c.
Phylogenomic databases
eggNOG	fuNOG05723.
GeneTree	EFGT00070000008712.
HOGENOM	HBG396709.
OMA	WCCHCGY.
OrthoDB	EOG4CRQ75.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004014-MONOMER.
BRENDA	2.7.11.13. 5615.
Gene expression databases
ArrayExpress	P36583.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011072. HR1_rho-bd. IPR011009. Kinase-like_dom. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Gene3D	G3DSA:1.10.287.160. HR1_rho-bd. 1 hit.
KO	K02677.
Pfam	PF00130. C1_1. 2 hits. PF02185. HR1. 2 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00742. Hr1. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit. SSF46585. PKN_effector. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCK2_SCHPO

Accession

Primary (citable) accession number: P36583
Secondary accession number(s): Q9UU42

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program