P36582 (PCK1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase C-like 1
EC=2.7.11.13

Gene names

Name:	pck1
ORF Names:	SPAC17G8.14c, SPAC22H10.01c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	988 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the control of the cell shape. Target of the inhibitor staurosporine.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. Contains 2 REM (Hr1) repeats.

Ontologies

Keywords
Biological process	Cell shape
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of bipolar cell growth Inferred from mutant phenotype. Source: GeneDB_Spombe intracellular signal transduction Inferred from electronic annotation. Source: InterPro regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell wall (1->3)-beta-D-glucan biosynthetic process Inferred from mutant phenotype. Source: GeneDB_Spombe regulation of establishment or maintenance of cell polarity Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	cell division site Inferred from direct assay. Source: GeneDB_Spombe cytosol Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: GeneDB_Spombe protein kinase C activity Traceable author statement. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 988	988	Protein kinase C-like 1		PRO_0000055740
Regions
	Repeat	6 – 78	73	REM 1
	Repeat	119 – 192	74	REM 2
	Domain	664 – 923	260	Protein kinase
	Domain	924 – 988	65	AGC-kinase C-terminal
	Zinc finger	413 – 461	49	Phorbol-ester/DAG-type 1
	Zinc finger	480 – 530	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	670 – 678	9	ATP By similarity
Sites
	Active site	789	1	Proton acceptor By similarity
	Binding site	693	1	ATP By similarity
Experimental info
	Sequence conflict	27 – 35	9	AMVASTKNP → SNGGFDGES Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36582 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 0969BDEC1AB43C4E	FASTA	988	111,784
	10 20 30 40 50 60 MVQLDDALQD AYKKVEREES LILGAKAMVA STKNPEVKRR LESNIAVSEN NIKYLRERID 70 80 90 100 110 120 ALKVESGSER ESQSDKDSSK KYSDSAKSTN SDDHLLSYNR SAFDLFNSEK PLSPEKISTM 130 140 150 160 170 180 LQHLQMRLSI EQQCVSGIEK IMSLYSKEQK DKTDVIIKLK EGKQKVNLLK RSLKRYNELH 190 200 210 220 230 240 IPFDISTPSS EEKQQASGLN FRGLAKPISG TLKVTIHSLR NIEHTSFLQT HSFTMPSYAV 250 260 270 280 290 300 LYVDDAQVAK SRISQTDTWD ETFIFDVHRA KEFQIIIYEK KKDFDIPIAL ILIPTTIIAE 310 320 330 340 350 360 ELRRKRNIQE MSETSWKPSI AESASRSDEK GSKSDPINAP NSSSSISTNS PLAPTAYYKL 370 380 390 400 410 420 LSKSWLSLEP VGQICISLSF SKRTTKRQFP ETGLGRQGAI RQKKEDVVAS QVGHQFVQRQ 430 440 450 460 470 480 FYQIMRCAVC AELFSYSPGL QCENCSFVCH KKCVTKVLAS CIAQSNSEKS DFGGLRYRIP 490 500 510 520 530 540 HRFEPFNSLG AQWCAHCGFF LPLRRKDCFK CVECGITCHG QCAHLIPDYC GMSNDLKHQL 550 560 570 580 590 600 LTELEVSKRP KKPELPNQEN KTTNEKVYRK PLSSQNTFDT LPTISQGLLA ATQPVTSVLN 610 620 630 640 650 660 TSPLPKTPEK DRSLNVTPSS STPTPASVLA PPSSASLSSS KDANRSVPES PRREKKNRVT 670 680 690 700 710 720 LDDFTFLAVL GKGNFGKVML AEYKVNKKFY AIKVLKKEAI LKNEELESLK TEKHVFEVAN 730 740 750 760 770 780 KEKHPFLLNL FASFQTSTRV YFVMEYILGG DLMVHIQRQQ FSVKRARFYG AEVCLALKYF 790 800 810 820 830 840 HENGIAYRDL KLDNILLCPD GHIRIADYGL CKENMLLGNT TSTFCGTPEF MAPEILLEQQ 850 860 870 880 890 900 YSKDVDWWAF GVLMYQMLLG QSPFKGEDEE EIFDAILSDE PLFPINMPAD AVSLLRGLLT 910 920 930 940 950 960 RDPNQRLGSG PKDANEVMAH PFFASIVWDD LYNKLYEPSY KPLINDPRDL NNFDEEFTSA 970 980 CPTLTPVNTV LTRQQQECFR GFSSFATE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control." Toda T., Shimanuki M., Yanagida M. EMBO J. 12:1987-1995(1993) [PubMed: 8491190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14337 Genomic DNA. Translation: BAA03267.1. CU329670 Genomic DNA. Translation: CAA93697.1.
PIR	S35362.
RefSeq	NP_593737.2. NM_001019168.1.
3D structure databases
ProteinModelPortal	P36582.
ModBase	Search...
Protein-protein interaction databases
STRING	P36582.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC17G8.14c.1; SPAC17G8.14c.1:pep; SPAC17G8.14c.
GeneID	2542293.
GenomeReviews	Gene locus pck1 in contig CU329670_GR.
KEGG	spo:SPAC17G8.14c.
NMPDR	fig|4896.1.peg.3707.
Organism-specific databases
GeneDB_Spombe	SPAC17G8.14c.
Phylogenomic databases
eggNOG	fuNOG05723.
GeneTree	EFGT00070000008712.
HOGENOM	HBG396709.
OrthoDB	EOG4Q887F.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001938-MONOMER.
BRENDA	2.7.11.13. 5615.
Gene expression databases
ArrayExpress	P36582.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011072. HR1_rho-bd. IPR011009. Kinase-like_dom. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Gene3D	G3DSA:1.10.287.160. HR1_rho-bd. 1 hit.
KO	K00924.
Pfam	PF00130. C1_1. 2 hits. PF02185. HR1. 2 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00742. Hr1. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit. SSF46585. PKN_effector. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCK1_SCHPO

Accession

Primary (citable) accession number: P36582

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program