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P36580

- ALF_SCHPO

UniProt

P36580 - ALF_SCHPO

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Protein

Fructose-bisphosphate aldolase

Gene
fba1, SPBC19C2.07
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity.

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611Glyceraldehyde 3-phosphate By similarity
Active sitei108 – 1081Proton donor By similarity
Metal bindingi109 – 1091Zinc 1; catalytic By similarity
Metal bindingi143 – 1431Zinc 2 By similarity
Metal bindingi173 – 1731Zinc 2 By similarity
Metal bindingi225 – 2251Zinc 1; catalytic By similarity
Binding sitei226 – 2261Dihydroxyacetone phosphate; via amide nitrogen By similarity
Metal bindingi264 – 2641Zinc 1; catalytic By similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: PomBase
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. glycolytic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba1
ORF Names:SPBC19C2.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC19C2.07.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Fructose-bisphosphate aldolasePRO_0000178761Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei289 – 2891Phosphothreonine1 Publication
Modified residuei312 – 3121Phosphothreonine1 Publication
Modified residuei340 – 3401Phosphothreonine1 Publication
Modified residuei342 – 3421Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36580.
PaxDbiP36580.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

BioGridi277287. 14 interactions.
IntActiP36580. 3 interactions.
MINTiMINT-4688656.
STRINGi4896.SPBC19C2.07-1.

Structurei

3D structure databases

ProteinModelPortaliP36580.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni265 – 2673Dihydroxyacetone phosphate binding By similarity
Regioni286 – 2894Dihydroxyacetone phosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0191.
HOGENOMiHOG000227794.
KOiK01624.
OMAiCNDLHSA.
OrthoDBiEOG7HTHSN.
PhylomeDBiP36580.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36580-1 [UniParc]FASTAAdd to Basket

« Hide

MGILDIVPTG VITGDNVLKL FTYAREHGFA IPAINVTSSS TAIAALEAAR    50
EARSPIILQT SNGGAHFFAG KESSNEGQKA SIAGSIAAAH YIRSIAPFFG 100
VPVVMHSDHC AKKLLPWMDG MFEADEAYFK IHGEPLFSSH MLDLSEEPKK 150
ENIAQVKEYC KRAVPMKIWI EMEIGITGGE EDGVDNSHVS HTELYTQPED 200
IWDVYRELSS VTPYFSIAAA FGNVHGVYKP GNVKLQPALL GQHQAYVKEQ 250
LKTTNDKPVF FVFHGGSGSS VNEFRTGIKC GVVKVNIDTD TQFAYVEGVR 300
DYVLKYKDYL MTPVGNPEGA DKPNKKKFDP RVWIHEGEKT MTKRVLTALE 350
DFYTVNTL 358
Length:358
Mass (Da):39,570
Last modified:January 11, 2001 - v2
Checksum:i50111C1C0E6DD05E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131T → A in BAA04237. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17415 Genomic DNA. Translation: BAA04237.1.
CU329671 Genomic DNA. Translation: CAB52034.1.
PIRiT39798.
T43289.
RefSeqiNP_595692.1. NM_001021589.2.

Genome annotation databases

EnsemblFungiiSPBC19C2.07.1; SPBC19C2.07.1:pep; SPBC19C2.07.
GeneIDi2540767.
KEGGispo:SPBC19C2.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17415 Genomic DNA. Translation: BAA04237.1 .
CU329671 Genomic DNA. Translation: CAB52034.1 .
PIRi T39798.
T43289.
RefSeqi NP_595692.1. NM_001021589.2.

3D structure databases

ProteinModelPortali P36580.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277287. 14 interactions.
IntActi P36580. 3 interactions.
MINTi MINT-4688656.
STRINGi 4896.SPBC19C2.07-1.

Proteomic databases

MaxQBi P36580.
PaxDbi P36580.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC19C2.07.1 ; SPBC19C2.07.1:pep ; SPBC19C2.07 .
GeneIDi 2540767.
KEGGi spo:SPBC19C2.07.

Organism-specific databases

PomBasei SPBC19C2.07.

Phylogenomic databases

eggNOGi COG0191.
HOGENOMi HOG000227794.
KOi K01624.
OMAi CNDLHSA.
OrthoDBi EOG7HTHSN.
PhylomeDBi P36580.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00183 .

Miscellaneous databases

NextBioi 20801886.
PROi P36580.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view ]
Pfami PF01116. F_bP_aldolase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsi TIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and nucleotide sequencing of Schizosaccharomyces pombe homologue of the class II fructose-1,6-bisphosphate aldolase gene."
    Mutoh N., Hayashi Y.
    Biochim. Biophys. Acta 1183:550-552(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-289; THR-312; THR-340 AND THR-342, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiALF_SCHPO
AccessioniPrimary (citable) accession number: P36580
Secondary accession number(s): Q9UUD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: June 11, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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