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P36580

- ALF_SCHPO

UniProt

P36580 - ALF_SCHPO

Protein

Fructose-bisphosphate aldolase

Gene

fba1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (11 Jan 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611Glyceraldehyde 3-phosphateBy similarity
    Active sitei108 – 1081Proton donorBy similarity
    Metal bindingi109 – 1091Zinc 1; catalyticBy similarity
    Metal bindingi143 – 1431Zinc 2By similarity
    Metal bindingi173 – 1731Zinc 2By similarity
    Metal bindingi225 – 2251Zinc 1; catalyticBy similarity
    Binding sitei226 – 2261Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi264 – 2641Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: PomBase
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: PomBase

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase (EC:4.1.2.13)
    Short name:
    FBP aldolase
    Short name:
    FBPA
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase
    Gene namesi
    Name:fba1
    ORF Names:SPBC19C2.07
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC19C2.07.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. nucleus Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Fructose-bisphosphate aldolasePRO_0000178761Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei289 – 2891Phosphothreonine1 Publication
    Modified residuei312 – 3121Phosphothreonine1 Publication
    Modified residuei340 – 3401Phosphothreonine1 Publication
    Modified residuei342 – 3421Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP36580.
    PaxDbiP36580.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi277287. 14 interactions.
    IntActiP36580. 3 interactions.
    MINTiMINT-4688656.
    STRINGi4896.SPBC19C2.07-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP36580.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni265 – 2673Dihydroxyacetone phosphate bindingBy similarity
    Regioni286 – 2894Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0191.
    HOGENOMiHOG000227794.
    KOiK01624.
    OMAiCNDLHSA.
    OrthoDBiEOG7HTHSN.
    PhylomeDBiP36580.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36580-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGILDIVPTG VITGDNVLKL FTYAREHGFA IPAINVTSSS TAIAALEAAR    50
    EARSPIILQT SNGGAHFFAG KESSNEGQKA SIAGSIAAAH YIRSIAPFFG 100
    VPVVMHSDHC AKKLLPWMDG MFEADEAYFK IHGEPLFSSH MLDLSEEPKK 150
    ENIAQVKEYC KRAVPMKIWI EMEIGITGGE EDGVDNSHVS HTELYTQPED 200
    IWDVYRELSS VTPYFSIAAA FGNVHGVYKP GNVKLQPALL GQHQAYVKEQ 250
    LKTTNDKPVF FVFHGGSGSS VNEFRTGIKC GVVKVNIDTD TQFAYVEGVR 300
    DYVLKYKDYL MTPVGNPEGA DKPNKKKFDP RVWIHEGEKT MTKRVLTALE 350
    DFYTVNTL 358
    Length:358
    Mass (Da):39,570
    Last modified:January 11, 2001 - v2
    Checksum:i50111C1C0E6DD05E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131T → A in BAA04237. (PubMed:8286404)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17415 Genomic DNA. Translation: BAA04237.1.
    CU329671 Genomic DNA. Translation: CAB52034.1.
    PIRiT39798.
    T43289.
    RefSeqiNP_595692.1. NM_001021589.2.

    Genome annotation databases

    EnsemblFungiiSPBC19C2.07.1; SPBC19C2.07.1:pep; SPBC19C2.07.
    GeneIDi2540767.
    KEGGispo:SPBC19C2.07.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17415 Genomic DNA. Translation: BAA04237.1 .
    CU329671 Genomic DNA. Translation: CAB52034.1 .
    PIRi T39798.
    T43289.
    RefSeqi NP_595692.1. NM_001021589.2.

    3D structure databases

    ProteinModelPortali P36580.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277287. 14 interactions.
    IntActi P36580. 3 interactions.
    MINTi MINT-4688656.
    STRINGi 4896.SPBC19C2.07-1.

    Proteomic databases

    MaxQBi P36580.
    PaxDbi P36580.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC19C2.07.1 ; SPBC19C2.07.1:pep ; SPBC19C2.07 .
    GeneIDi 2540767.
    KEGGi spo:SPBC19C2.07.

    Organism-specific databases

    PomBasei SPBC19C2.07.

    Phylogenomic databases

    eggNOGi COG0191.
    HOGENOMi HOG000227794.
    KOi K01624.
    OMAi CNDLHSA.
    OrthoDBi EOG7HTHSN.
    PhylomeDBi P36580.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .

    Miscellaneous databases

    NextBioi 20801886.
    PROi P36580.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequencing of Schizosaccharomyces pombe homologue of the class II fructose-1,6-bisphosphate aldolase gene."
      Mutoh N., Hayashi Y.
      Biochim. Biophys. Acta 1183:550-552(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-289; THR-312; THR-340 AND THR-342, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiALF_SCHPO
    AccessioniPrimary (citable) accession number: P36580
    Secondary accession number(s): Q9UUD3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3