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Protein

Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase

Gene

cobT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).

Catalytic activityi

Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate.

Pathwayi: alpha-ribazole biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT)
  2. Adenosylcobalamin/alpha-ribazole phosphatase (cobC)
This subpathway is part of the pathway alpha-ribazole biosynthesis, which is itself part of Nucleoside biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole, the pathway alpha-ribazole biosynthesis and in Nucleoside biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei318Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cobalamin biosynthetic process Source: EcoliWiki
  • nucleoside biosynthetic process Source: InterPro

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCobalamin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:DMBPPRIBOSYLTRANS-MONOMER
UniPathwayiUPA00061; UER00516

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC:2.4.2.21)
Short name:
NN:DBI PRT
Alternative name(s):
N(1)-alpha-phosphoribosyltransferase
Gene namesi
Name:cobT
Ordered Locus Names:b1991, JW1969
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12151 cobT

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001670481 – 359Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferaseAdd BLAST359

Proteomic databases

PaxDbiP36562
PRIDEiP36562

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4262028, 15 interactors
IntActiP36562, 5 interactors
STRINGi316385.ECDH10B_2135

Structurei

3D structure databases

ProteinModelPortaliP36562
SMRiP36562
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CobT family.Curated

Phylogenomic databases

eggNOGiENOG4105CUP Bacteria
COG2038 LUCA
HOGENOMiHOG000263499
InParanoidiP36562
KOiK00768
OMAiATFGGFE
PhylomeDBiP36562

Family and domain databases

CDDicd02439 DMB-PRT_CobT, 1 hit
Gene3Di1.10.1610.10, 2 hits
HAMAPiMF_00230 CobT, 1 hit
InterProiView protein in InterPro
IPR003200 Nict_dMeBzImd_PRibTrfase-like
IPR023195 Nict_dMeBzImd_PRibTrfase_N
IPR017846 Nict_dMeBzImd_PRibTrfase_pro
IPR036087 Nict_dMeBzImd_PRibTrfase_sf
PfamiView protein in Pfam
PF02277 DBI_PRT, 1 hit
SUPFAMiSSF52733 SSF52733, 1 hit
TIGRFAMsiTIGR03160 cobT_DBIPRT, 1 hit

Sequencei

Sequence statusi: Complete.

P36562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQILADLLNT IPAIDSTAMS RAQRHIDGLL KPVGSLGKLE VLAIQLAGMP
60 70 80 90 100
GLNGIPHVGK KAVLVMCADH GVWEEGVAIS PKEVTAIQAE NMTRGTTGVC
110 120 130 140 150
VLAEQAGANV HVIDVGIDTA EPIPGLINMR VARGSGNIAS APAMSRRQAE
160 170 180 190 200
KLLLDVICYT QELAKNGVTL FGVGELGMAN TTPAAAIVST ITGRDPEEVV
210 220 230 240 250
GIGANLPTDK LANKIDVVRR AITLNQPNPQ DGVDVLAKVG GFDLVGIAGV
260 270 280 290 300
MLGAASCGLP VLLDGFLSYA AALAACQMSP AIKPYLIPSH LSAEKGARIA
310 320 330 340 350
LSHLGLEPYL NMEMRLGEGS GAALAMPIIE AACAIYNNMG ELAASNIVLP

GNTTSDLNS
Length:359
Mass (Da):36,987
Last modified:November 1, 1997 - v3
Checksum:i6058C6465FAE55EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271A → L in AAA78908 (PubMed:7592411).Curated1
Sequence conflicti301L → V in AAA78908 (PubMed:7592411).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA Translation: AAA78908.1
U00096 Genomic DNA Translation: AAC75052.1
AP009048 Genomic DNA Translation: BAA15808.1
L25054 Genomic DNA Translation: AAA56876.1
PIRiF64963
RefSeqiNP_416495.1, NC_000913.3
WP_001166160.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75052; AAC75052; b1991
BAA15808; BAA15808; BAA15808
GeneIDi946517
KEGGiecj:JW1969
eco:b1991
PATRICifig|1411691.4.peg.263

Similar proteinsi

Entry informationi

Entry nameiCOBT_ECOLI
AccessioniPrimary (citable) accession number: P36562
Secondary accession number(s): P78075
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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