P36562 (COBT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Short name=NN:DBI PRT
EC=2.4.2.21

Alternative name(s):
N(1)-alpha-phosphoribosyltransferase

Gene names

Name:	cobT
Ordered Locus Names:	b1991, JW1969

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB). HAMAP MF_00230
Catalytic activity	Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate. HAMAP MF_00230
Pathway	Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2. HAMAP MF_00230
Subunit structure	Homodimer By similarity. HAMAP MF_00230
Sequence similarities	Belongs to the CobT family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from genetic interaction Ref.1. Source: EcoCyc nucleoside biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Inferred from genetic interaction Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase HAMAP MF_00230		PRO_0000167048
Sites
	Active site	318	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	271	1	A → L in AAA78908. Ref.1
	Sequence conflict	301	1	L → V in AAA78908. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36562 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 6058C6465FAE55EF	FASTA	359	36,987
	10 20 30 40 50 60 MQILADLLNT IPAIDSTAMS RAQRHIDGLL KPVGSLGKLE VLAIQLAGMP GLNGIPHVGK 70 80 90 100 110 120 KAVLVMCADH GVWEEGVAIS PKEVTAIQAE NMTRGTTGVC VLAEQAGANV HVIDVGIDTA 130 140 150 160 170 180 EPIPGLINMR VARGSGNIAS APAMSRRQAE KLLLDVICYT QELAKNGVTL FGVGELGMAN 190 200 210 220 230 240 TTPAAAIVST ITGRDPEEVV GIGANLPTDK LANKIDVVRR AITLNQPNPQ DGVDVLAKVG 250 260 270 280 290 300 GFDLVGIAGV MLGAASCGLP VLLDGFLSYA AALAACQMSP AIKPYLIPSH LSAEKGARIA 310 320 330 340 350 LSHLGLEPYL NMEMRLGEGS GAALAMPIIE AACAIYNNMG ELAASNIVLP GNTTSDLNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli." Lawrence J.G., Roth J.R. J. Bacteriol. 177:6371-6380(1995) [PubMed: 7592411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Location of cobS and cobT gene sequences on the physical map of the Escherichia coli K-12 chromosome." Collins C.M., Gutman D.M., Isaza J. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-188. Strain: K12 / DH5-alpha.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33333 Genomic DNA. Translation: AAA78908.1. U00096 Genomic DNA. Translation: AAC75052.1. AP009048 Genomic DNA. Translation: BAA15808.1. L25054 Genomic DNA. Translation: AAA56876.1.
PIR	F64963.
RefSeq	NP_416495.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P36562.
SMR	P36562. Positions 4-350.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36562. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000281; EBESCP00000000281; EBESCG00000000234. EBESCT00000000282; EBESCP00000000282; EBESCG00000000234. EBESCT00000017248; EBESCP00000016539; EBESCG00000016307.
GeneID	946517.
GenomeReviews	Gene locus JW1969 in contig AP009048_GR. Gene locus b1991 in contig U00096_GR.
KEGG	ecj:JW1969. eco:b1991.
PATRIC	32119313. VBIEscCol129921_2066.
Organism-specific databases
EchoBASE	EB2071.
EcoGene	EG12151. cobT.
Phylogenomic databases
eggNOG	COG2038.
GeneTree	EBGT00050000010481.
HOGENOM	HBG634399.
OMA	FICSVAA.
PhylomeDB	P36562.
ProtClustDB	PRK00105.
Enzyme and pathway databases
BioCyc	EcoCyc:DMBPPRIBOSYLTRANS-MONOMER.
Gene expression databases
Genevestigator	P36562.
Family and domain databases
HAMAP	MF_00230. CobT. [Tree]
InterPro	IPR003200. Nict_dMeBzImd_PRibTrfase-like. IPR023195. Nict_dMeBzImd_PRibTrfase_N. IPR017846. Nict_dMeBzImd_PRibTrfase_pro. [Graphical view]
Gene3D	G3DSA:1.10.1610.10. Nict_dMeBzImd_PRibTrfase_N. 1 hit.
KO	K00768.
Pfam	PF02277. DBI_PRT. 1 hit. [Graphical view]
SUPFAM	SSF52733. NN:DBI_PRT. 1 hit.
TIGRFAMs	TIGR03160. CobT_DBIPRT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBT_ECOLI

Accession

Primary (citable) accession number: P36562
Secondary accession number(s): P78075

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program