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Protein

Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase

Gene

cobT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).

Catalytic activityi

Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate.

Pathwayi: alpha-ribazole biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT)
  2. Adenosylcobalamin/alpha-ribazole phosphatase (cobC)
This subpathway is part of the pathway alpha-ribazole biosynthesis, which is itself part of Nucleoside biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole, the pathway alpha-ribazole biosynthesis and in Nucleoside biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei318Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cobalamin biosynthetic process Source: EcoliWiki
  • nucleoside biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:DMBPPRIBOSYLTRANS-MONOMER.
ECOL316407:JW1969-MONOMER.
UniPathwayiUPA00061; UER00516.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC:2.4.2.21)
Short name:
NN:DBI PRT
Alternative name(s):
N(1)-alpha-phosphoribosyltransferase
Gene namesi
Name:cobT
Ordered Locus Names:b1991, JW1969
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12151. cobT.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001670481 – 359Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferaseAdd BLAST359

Proteomic databases

PaxDbiP36562.
PRIDEiP36562.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4262028. 15 interactors.
IntActiP36562. 5 interactors.
STRINGi511145.b1991.

Structurei

3D structure databases

ProteinModelPortaliP36562.
SMRiP36562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CobT family.Curated

Phylogenomic databases

eggNOGiENOG4105CUP. Bacteria.
COG2038. LUCA.
HOGENOMiHOG000263499.
InParanoidiP36562.
KOiK00768.
OMAiMNNMATF.
PhylomeDBiP36562.

Family and domain databases

CDDicd02439. DMB-PRT_CobT. 1 hit.
Gene3Di1.10.1610.10. 1 hit.
HAMAPiMF_00230. CobT. 1 hit.
InterProiIPR003200. Nict_dMeBzImd_PRibTrfase-like.
IPR023195. Nict_dMeBzImd_PRibTrfase_N.
IPR017846. Nict_dMeBzImd_PRibTrfase_pro.
[Graphical view]
PfamiPF02277. DBI_PRT. 1 hit.
[Graphical view]
SUPFAMiSSF52733. SSF52733. 1 hit.
TIGRFAMsiTIGR03160. cobT_DBIPRT. 1 hit.

Sequencei

Sequence statusi: Complete.

P36562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQILADLLNT IPAIDSTAMS RAQRHIDGLL KPVGSLGKLE VLAIQLAGMP
60 70 80 90 100
GLNGIPHVGK KAVLVMCADH GVWEEGVAIS PKEVTAIQAE NMTRGTTGVC
110 120 130 140 150
VLAEQAGANV HVIDVGIDTA EPIPGLINMR VARGSGNIAS APAMSRRQAE
160 170 180 190 200
KLLLDVICYT QELAKNGVTL FGVGELGMAN TTPAAAIVST ITGRDPEEVV
210 220 230 240 250
GIGANLPTDK LANKIDVVRR AITLNQPNPQ DGVDVLAKVG GFDLVGIAGV
260 270 280 290 300
MLGAASCGLP VLLDGFLSYA AALAACQMSP AIKPYLIPSH LSAEKGARIA
310 320 330 340 350
LSHLGLEPYL NMEMRLGEGS GAALAMPIIE AACAIYNNMG ELAASNIVLP

GNTTSDLNS
Length:359
Mass (Da):36,987
Last modified:November 1, 1997 - v3
Checksum:i6058C6465FAE55EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271A → L in AAA78908 (PubMed:7592411).Curated1
Sequence conflicti301L → V in AAA78908 (PubMed:7592411).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA. Translation: AAA78908.1.
U00096 Genomic DNA. Translation: AAC75052.1.
AP009048 Genomic DNA. Translation: BAA15808.1.
L25054 Genomic DNA. Translation: AAA56876.1.
PIRiF64963.
RefSeqiNP_416495.1. NC_000913.3.
WP_001166160.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75052; AAC75052; b1991.
BAA15808; BAA15808; BAA15808.
GeneIDi946517.
KEGGiecj:JW1969.
eco:b1991.
PATRICi32119313. VBIEscCol129921_2066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA. Translation: AAA78908.1.
U00096 Genomic DNA. Translation: AAC75052.1.
AP009048 Genomic DNA. Translation: BAA15808.1.
L25054 Genomic DNA. Translation: AAA56876.1.
PIRiF64963.
RefSeqiNP_416495.1. NC_000913.3.
WP_001166160.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP36562.
SMRiP36562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262028. 15 interactors.
IntActiP36562. 5 interactors.
STRINGi511145.b1991.

Proteomic databases

PaxDbiP36562.
PRIDEiP36562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75052; AAC75052; b1991.
BAA15808; BAA15808; BAA15808.
GeneIDi946517.
KEGGiecj:JW1969.
eco:b1991.
PATRICi32119313. VBIEscCol129921_2066.

Organism-specific databases

EchoBASEiEB2071.
EcoGeneiEG12151. cobT.

Phylogenomic databases

eggNOGiENOG4105CUP. Bacteria.
COG2038. LUCA.
HOGENOMiHOG000263499.
InParanoidiP36562.
KOiK00768.
OMAiMNNMATF.
PhylomeDBiP36562.

Enzyme and pathway databases

UniPathwayiUPA00061; UER00516.
BioCyciEcoCyc:DMBPPRIBOSYLTRANS-MONOMER.
ECOL316407:JW1969-MONOMER.

Miscellaneous databases

PROiP36562.

Family and domain databases

CDDicd02439. DMB-PRT_CobT. 1 hit.
Gene3Di1.10.1610.10. 1 hit.
HAMAPiMF_00230. CobT. 1 hit.
InterProiIPR003200. Nict_dMeBzImd_PRibTrfase-like.
IPR023195. Nict_dMeBzImd_PRibTrfase_N.
IPR017846. Nict_dMeBzImd_PRibTrfase_pro.
[Graphical view]
PfamiPF02277. DBI_PRT. 1 hit.
[Graphical view]
SUPFAMiSSF52733. SSF52733. 1 hit.
TIGRFAMsiTIGR03160. cobT_DBIPRT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOBT_ECOLI
AccessioniPrimary (citable) accession number: P36562
Secondary accession number(s): P78075
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.