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Protein

Adenosylcobinamide-GDP ribazoletransferase

Gene

cobS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate (By similarity).By similarity

Catalytic activityi

Adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin.
Adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate = GMP + adenosylcobalamin 5'-phosphate.

Cofactori

Mg2+By similarity

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Cob(I)yrinic acid a,c-diamide adenosyltransferase (btuR)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
  6. Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • cobalamin biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciEcoCyc:COBS-MONOMER.
ECOL316407:JW1970-MONOMER.
MetaCyc:COBS-MONOMER.
UniPathwayiUPA00148; UER00238.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylcobinamide-GDP ribazoletransferase (EC:2.7.8.26)
Alternative name(s):
Cobalamin synthase
Cobalamin-5'-phosphate synthase
Gene namesi
Name:cobS
Ordered Locus Names:b1992, JW1970
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12150. cobS.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Transmembranei59 – 79HelicalSequence analysisAdd BLAST21
Transmembranei113 – 133HelicalSequence analysisAdd BLAST21
Transmembranei138 – 158HelicalSequence analysisAdd BLAST21
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001468761 – 247Adenosylcobinamide-GDP ribazoletransferaseAdd BLAST247

Proteomic databases

PaxDbiP36561.
PRIDEiP36561.

Expressioni

Inductioni

By cobinamide.

Interactioni

Protein-protein interaction databases

BioGridi4260409. 9 interactors.
IntActiP36561. 5 interactors.
STRINGi511145.b1992.

Structurei

3D structure databases

ProteinModelPortaliP36561.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CobS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108UHF. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
InParanoidiP36561.
KOiK02233.
OMAiGAWNWLV.
PhylomeDBiP36561.

Family and domain databases

HAMAPiMF_00719. CobS. 1 hit.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.

Sequencei

Sequence statusi: Complete.

P36561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLFWAMLS FITRLPVPRR WSQGLDFEHY SRGIITFPLI GLLLGAISGL
60 70 80 90 100
VFMVLQAWCG APLAALFSVL VLVLMTGGFH LDGLADTCDG VFSARSRDRM
110 120 130 140 150
LEIMRDSRLG THGGLALIFV VLAKILVLSE LALRGESILA SLAAACAVSR
160 170 180 190 200
GTAALLMYRH RYAREEGLGN VFIGKIDGRQ TCVTLGLAAI FAAVLLPGMH
210 220 230 240
GVAAMVVTMV AIFILGQLLK RTLGGQTGDT LGAAIELGEL VFLLALL
Length:247
Mass (Da):26,386
Last modified:November 1, 1997 - v3
Checksum:i8D48B8C91428F13F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48 – 49SG → RR (PubMed:7592411).Curated2
Sequence conflicti48 – 49SG → RR (Ref. 5) Curated2
Sequence conflicti145A → R in AAA56875 (Ref. 5) Curated1
Sequence conflicti203A → T in AAA56875 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA. Translation: AAA78907.1.
U00096 Genomic DNA. Translation: AAC75053.1.
AP009048 Genomic DNA. Translation: BAA15809.1.
L25054 Genomic DNA. Translation: AAA56875.1.
PIRiG64963.
RefSeqiNP_416496.1. NC_000913.3.
WP_001326708.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75053; AAC75053; b1992.
BAA15809; BAA15809; BAA15809.
GeneIDi946520.
KEGGiecj:JW1970.
eco:b1992.
PATRICi32119315. VBIEscCol129921_2067.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA. Translation: AAA78907.1.
U00096 Genomic DNA. Translation: AAC75053.1.
AP009048 Genomic DNA. Translation: BAA15809.1.
L25054 Genomic DNA. Translation: AAA56875.1.
PIRiG64963.
RefSeqiNP_416496.1. NC_000913.3.
WP_001326708.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP36561.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260409. 9 interactors.
IntActiP36561. 5 interactors.
STRINGi511145.b1992.

Proteomic databases

PaxDbiP36561.
PRIDEiP36561.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75053; AAC75053; b1992.
BAA15809; BAA15809; BAA15809.
GeneIDi946520.
KEGGiecj:JW1970.
eco:b1992.
PATRICi32119315. VBIEscCol129921_2067.

Organism-specific databases

EchoBASEiEB2070.
EcoGeneiEG12150. cobS.

Phylogenomic databases

eggNOGiENOG4108UHF. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
InParanoidiP36561.
KOiK02233.
OMAiGAWNWLV.
PhylomeDBiP36561.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00238.
BioCyciEcoCyc:COBS-MONOMER.
ECOL316407:JW1970-MONOMER.
MetaCyc:COBS-MONOMER.

Miscellaneous databases

PROiP36561.

Family and domain databases

HAMAPiMF_00719. CobS. 1 hit.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOBS_ECOLI
AccessioniPrimary (citable) accession number: P36561
Secondary accession number(s): P76354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.