Reviewed, UniProtKB/Swiss-Prot P36557 (BASS_SALTY)

Names and origin

Protein names

Recommended name:
    Sensor protein basS
    EC=2.7.13.3

Gene names

Name:	basS
Synonyms:	parB, pmrB
Ordered Locus Names:	STM4291

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Member of the two-component regulatory system basS/basR. Autophosphorylates and activates basR by phosphorylation. Plays a role in the adaptation of the organism to the host environment, in particular to neutrophils, and therefore it plays a role in virulence as well. Ref.3
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Induction	The eptA-basRS operon is positively autoregulated by basR under high iron or aluminum concentration conditions. Ref.3
Post-translational modification	Autophosphorylated. Ref.3
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system Virulence
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Sensor protein basS		PRO_0000074702
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Potential
	Topological domain	35 – 64	30	Periplasmic Potential
	Transmembrane	65 – 88	24	Potential
	Topological domain	89 – 356	268	Cytoplasmic Potential
	Domain	89 – 141	53	HAMP
	Domain	149 – 356	208	Histidine kinase
Amino acid modifications
	Modified residue	152	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36557-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 953234B125727BBD	FASTA	356	40,262
	10 20 30 40 50 60 MRFQRRAMTL RQRLMLTIGL ILLVFQLIST FWLWHESTEQ IQLFEQALRD NRNNDRHIMH 70 80 90 100 110 120 EIREAVASLI VPGVFMVSLT LLICYQAVRR ITRPLAELQK ELEARTADNL APIAIHSSTL 130 140 150 160 170 180 EIESVVSAIN QLVTRLTTTL DNERLFTADV AHELRTPLSG VRLHLELLSK THNVDVAPLI 190 200 210 220 230 240 ARLDQMMDSV SQLLQLARVG QSFSSGNYQE VKLLEDVILP SYDELNTMLE TRQQTLLLPE 250 260 270 280 290 300 SAADVVVRGD ATLLRMLLRN LVENAHRYSP EGTHITIHIS ADPDAIMAVE DEGPGIDESK 310 320 330 340 350 CGKLSEAFVR MDSRYGGIGL GLSIVSRITQ LHQGQFFLQN RTERTGTRAW VLLKKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Spontaneous pmrA mutants of Salmonella typhimurium LT2 define a new two-component regulatory system with a possible role in virulence." Roland K.L., Martin L.E., Esther C.R., Spitznagel J.K. J. Bacteriol. 175:4154-4164(1993) [PubMed: 8391535] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Molecular characterization of the PmrA regulon." Woesten M.M.S.M., Groisman E.A. J. Biol. Chem. 274:27185-27190(1999) [PubMed: 10480935] [Abstract] Cited for: FUNCTION, INDUCTION, AUTOPHOSPHORYLATION. Strain: ATCC 14028s / SGSG 2262.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L13395 Genomic DNA. Translation: AAA72366.1. AE006468 Genomic DNA. Translation: AAL23115.1.
PIR	C40656.
RefSeq	NP_463156.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P36557.
Genome annotation databases
GeneID	1255817.
GenomeReviews	Gene locus STM4291 in contig AE006468_GR.
KEGG	stm:STM4291.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P36557.
OMA	SANIITE.
Enzyme and pathway databases
BioCyc	STYP99287:STM4291-MON.
BRENDA	2.7.13.3. 2.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BASS_SALTY

Accession

Primary (citable) accession number: P36557

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 3, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)