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P36556 (BASR_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional regulatory protein BasR
Gene names
Name:basR
Synonyms:parA, pmrA
Ordered Locus Names:STM4292
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin. Represses the transcription of pmrD. Plays a role in the adaptation of the organism to the host environment, in particular to neutrophils, and therefore it plays a role in virulence as well. Ref.5 Ref.7

Subunit structure

Homodimer. Ref.5

Subcellular location

Cytoplasm Potential.

Induction

The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions. Ref.2 Ref.4 Ref.5 Ref.7

Post-translational modification

Phosphorylated by BasS. Ref.5 Ref.6

Miscellaneous

The activity of the BasR protein is controlled at a post-transcriptional level by the PmrD protein, under low Mg2+ concentration conditions.

Sequence similarities

Contains 1 response regulatory domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 222222Transcriptional regulatory protein BasR
PRO_0000081024

Regions

Domain1 – 116116Response regulatory

Amino acid modifications

Modified residue5114-aspartylphosphate

Natural variations

Natural variant811H → R in strain: LT2 / SGSC1412 and S4194; resistance to polymyxin B and neutrophil proteins.

Experimental info

Mutagenesis511D → A: No activation of BasR regulated genes. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P36556 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 3734CC2034070533

FASTA22225,035
        10         20         30         40         50         60 
MKILIVEDDT LLLQGLILAA QTEGYACDGV STARAAEHSL ESGHYSLMVL DLGLPDEDGL 

        70         80         90        100        110        120 
HFLTRIRQKK YTLPVLILTA HDTLNDRITG LDVGADDYLV KPFALEELHA RIRALLRRHN 

       130        140        150        160        170        180 
NQGESELTVG NLTLNIGRHQ AWRDGQELTL TPKEYALLSR LMLKAGSPVH REILYNDIYN 

       190        200        210        220 
WDNEPSTNTL EVHIHNLRDK VGKSRIRTVR GFGYMLVATE ES 

« Hide

References

« Hide 'large scale' references
[1]"Spontaneous pmrA mutants of Salmonella typhimurium LT2 define a new two-component regulatory system with a possible role in virulence."
Roland K.L., Martin L.E., Esther C.R., Spitznagel J.K.
J. Bacteriol. 175:4154-4164(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT2.
[2]"Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes."
Winfield M.D., Groisman E.A.
Proc. Natl. Acad. Sci. U.S.A. 101:17162-17167(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REGULATION BY PMRD.
Strain: S4194.
[3]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[4]"PhoP-PhoQ activates transcription of pmrAB, encoding a two-component regulatory system involved in Salmonella typhimurium antimicrobial peptide resistance."
Gunn J.S., Miller S.I.
J. Bacteriol. 178:6857-6864(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: REGULATION.
Strain: ATCC 14028s / SGSG 2262.
[5]"Molecular characterization of the PmrA regulon."
Woesten M.M.S.M., Groisman E.A.
J. Biol. Chem. 274:27185-27190(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, PHOSPHORYLATION, SUBUNIT.
Strain: ATCC 14028s / SGSG 2262.
[6]"A small protein that mediates the activation of a two-component system by another two-component system."
Kox L.F.F., Woesten M.M.S.M., Groisman E.A.
EMBO J. 19:1861-1872(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ASP-51, PHOSPHORYLATION AT ASP-51.
Strain: ATCC 14028s / SGSG 2262.
[7]"Closing the loop: the PmrA/PmrB two-component system negatively controls expression of its posttranscriptional activator PmrD."
Kato A., Latifi T., Groisman E.A.
Proc. Natl. Acad. Sci. U.S.A. 100:4706-4711(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, REGULATION BY PMRD.
Strain: ATCC 14028s / SGSG 2262.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L13395 Genomic DNA. Translation: AAA72365.1.
AY725348 Genomic DNA. Translation: AAV92795.1.
AE006468 Genomic DNA. Translation: AAL23116.1.
PIRB40656.
RefSeqNP_463157.1. NC_003197.1.

3D structure databases

ProteinModelPortalP36556.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM4292.

Proteomic databases

PaxDbP36556.
PRIDEP36556.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL23116; AAL23116; STM4292.
GeneID1255818.
KEGGstm:STM4292.
PATRIC32387493. VBISalEnt20916_4514.

Phylogenomic databases

eggNOGCOG0745.
HOGENOMHOG000034815.
KOK07771.
OMAGYMLRPA.
OrthoDBEOG6G4VQG.
ProtClustDBPRK10643.

Enzyme and pathway databases

BioCycSENT99287:GCTI-4323-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBASR_SALTY
AccessionPrimary (citable) accession number: P36556
Secondary accession number(s): Q5MN92
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families