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P36556

- BASR_SALTY

UniProt

P36556 - BASR_SALTY

Protein

Transcriptional regulatory protein BasR

Gene

basR

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin. Represses the transcription of pmrD. Plays a role in the adaptation of the organism to the host environment, in particular to neutrophils, and therefore it plays a role in virulence as well.2 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. phosphorelay response regulator activity Source: InterPro

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. response to antibiotic Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Antibiotic resistance, Transcription, Transcription regulation, Two-component regulatory system, Virulence

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-4323-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional regulatory protein BasR
    Gene namesi
    Name:basR
    Synonyms:parA, pmrA
    Ordered Locus Names:STM4292
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi51 – 511D → A: No activation of BasR regulated genes. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 222222Transcriptional regulatory protein BasRPRO_0000081024Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei51 – 5114-aspartylphosphate2 PublicationsPROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated by BasS.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP36556.
    PRIDEiP36556.

    Expressioni

    Inductioni

    The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM4292.

    Structurei

    3D structure databases

    ProteinModelPortaliP36556.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 116116Response regulatoryPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0745.
    HOGENOMiHOG000034815.
    KOiK07771.
    OMAiACDCAGT.
    OrthoDBiEOG6G4VQG.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36556-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKILIVEDDT LLLQGLILAA QTEGYACDGV STARAAEHSL ESGHYSLMVL    50
    DLGLPDEDGL HFLTRIRQKK YTLPVLILTA HDTLNDRITG LDVGADDYLV 100
    KPFALEELHA RIRALLRRHN NQGESELTVG NLTLNIGRHQ AWRDGQELTL 150
    TPKEYALLSR LMLKAGSPVH REILYNDIYN WDNEPSTNTL EVHIHNLRDK 200
    VGKSRIRTVR GFGYMLVATE ES 222
    Length:222
    Mass (Da):25,035
    Last modified:June 1, 1994 - v1
    Checksum:i3734CC2034070533
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti81 – 811H → R in strain: LT2 / SGSC1412 and S4194; resistance to polymyxin B and neutrophil proteins.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13395 Genomic DNA. Translation: AAA72365.1.
    AY725348 Genomic DNA. Translation: AAV92795.1.
    AE006468 Genomic DNA. Translation: AAL23116.1.
    PIRiB40656.
    RefSeqiNP_463157.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL23116; AAL23116; STM4292.
    GeneIDi1255818.
    KEGGistm:STM4292.
    PATRICi32387493. VBISalEnt20916_4514.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13395 Genomic DNA. Translation: AAA72365.1 .
    AY725348 Genomic DNA. Translation: AAV92795.1 .
    AE006468 Genomic DNA. Translation: AAL23116.1 .
    PIRi B40656.
    RefSeqi NP_463157.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P36556.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM4292.

    Proteomic databases

    PaxDbi P36556.
    PRIDEi P36556.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL23116 ; AAL23116 ; STM4292 .
    GeneIDi 1255818.
    KEGGi stm:STM4292.
    PATRICi 32387493. VBISalEnt20916_4514.

    Phylogenomic databases

    eggNOGi COG0745.
    HOGENOMi HOG000034815.
    KOi K07771.
    OMAi ACDCAGT.
    OrthoDBi EOG6G4VQG.

    Enzyme and pathway databases

    BioCyci SENT99287:GCTI-4323-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Spontaneous pmrA mutants of Salmonella typhimurium LT2 define a new two-component regulatory system with a possible role in virulence."
      Roland K.L., Martin L.E., Esther C.R., Spitznagel J.K.
      J. Bacteriol. 175:4154-4164(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LT2.
    2. "Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes."
      Winfield M.D., Groisman E.A.
      Proc. Natl. Acad. Sci. U.S.A. 101:17162-17167(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REGULATION BY PMRD.
      Strain: S4194.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    4. "PhoP-PhoQ activates transcription of pmrAB, encoding a two-component regulatory system involved in Salmonella typhimurium antimicrobial peptide resistance."
      Gunn J.S., Miller S.I.
      J. Bacteriol. 178:6857-6864(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: REGULATION.
      Strain: ATCC 14028s / SGSG 2262.
    5. "Molecular characterization of the PmrA regulon."
      Woesten M.M.S.M., Groisman E.A.
      J. Biol. Chem. 274:27185-27190(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, PHOSPHORYLATION, SUBUNIT.
      Strain: ATCC 14028s / SGSG 2262.
    6. "A small protein that mediates the activation of a two-component system by another two-component system."
      Kox L.F.F., Woesten M.M.S.M., Groisman E.A.
      EMBO J. 19:1861-1872(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ASP-51, PHOSPHORYLATION AT ASP-51.
      Strain: ATCC 14028s / SGSG 2262.
    7. "Closing the loop: the PmrA/PmrB two-component system negatively controls expression of its posttranscriptional activator PmrD."
      Kato A., Latifi T., Groisman E.A.
      Proc. Natl. Acad. Sci. U.S.A. 100:4706-4711(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, REGULATION BY PMRD.
      Strain: ATCC 14028s / SGSG 2262.

    Entry informationi

    Entry nameiBASR_SALTY
    AccessioniPrimary (citable) accession number: P36556
    Secondary accession number(s): Q5MN92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The activity of the BasR protein is controlled at a post-transcriptional level by the PmrD protein, under low Mg2+ concentration conditions.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3