P36556 (BASR_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein BasR

Gene names

Name:	basR
Synonyms:	parA, pmrA
Ordered Locus Names:	STM4292

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin. Represses the transcription of pmrD. Plays a role in the adaptation of the organism to the host environment, in particular to neutrophils, and therefore it plays a role in virulence as well. Ref.5 Ref.7
Subunit structure	Homodimer. Ref.5
Subcellular location	Cytoplasm Potential.
Induction	The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions. Ref.2 Ref.4 Ref.5 Ref.7
Post-translational modification	Phosphorylated by BasS. Ref.5 Ref.6
Miscellaneous	The activity of the BasR protein is controlled at a post-transcriptional level by the PmrD protein, under low Mg2+ concentration conditions.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Transcription Transcription regulation Two-component regulatory system Virulence
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Transcriptional regulatory protein BasR		PRO_0000081024
Regions
	Domain	1 – 116	116	Response regulatory
Amino acid modifications
	Modified residue	51	1	4-aspartylphosphate
Natural variations
	Natural variant	81	1	H → R in strain: LT2 / SGSC1412 and S4194; resistance to polymyxin B and neutrophil proteins.
Experimental info
	Mutagenesis	51	1	D → A: No activation of BasR regulated genes. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P36556 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 3734CC2034070533	FASTA	222	25,035
	10 20 30 40 50 60 MKILIVEDDT LLLQGLILAA QTEGYACDGV STARAAEHSL ESGHYSLMVL DLGLPDEDGL 70 80 90 100 110 120 HFLTRIRQKK YTLPVLILTA HDTLNDRITG LDVGADDYLV KPFALEELHA RIRALLRRHN 130 140 150 160 170 180 NQGESELTVG NLTLNIGRHQ AWRDGQELTL TPKEYALLSR LMLKAGSPVH REILYNDIYN 190 200 210 220 WDNEPSTNTL EVHIHNLRDK VGKSRIRTVR GFGYMLVATE ES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Spontaneous pmrA mutants of Salmonella typhimurium LT2 define a new two-component regulatory system with a possible role in virulence." Roland K.L., Martin L.E., Esther C.R., Spitznagel J.K. J. Bacteriol. 175:4154-4164(1993) [PubMed: 8391535] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes." Winfield M.D., Groisman E.A. Proc. Natl. Acad. Sci. U.S.A. 101:17162-17167(2004) [PubMed: 15569938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REGULATION BY PMRD. Strain: S4194.
[3]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[4]	"PhoP-PhoQ activates transcription of pmrAB, encoding a two-component regulatory system involved in Salmonella typhimurium antimicrobial peptide resistance." Gunn J.S., Miller S.I. J. Bacteriol. 178:6857-6864(1996) [PubMed: 8955307] [Abstract] Cited for: REGULATION. Strain: ATCC 14028s / SGSG 2262.
[5]	"Molecular characterization of the PmrA regulon." Woesten M.M.S.M., Groisman E.A. J. Biol. Chem. 274:27185-27190(1999) [PubMed: 10480935] [Abstract] Cited for: FUNCTION, INDUCTION, PHOSPHORYLATION, SUBUNIT. Strain: ATCC 14028s / SGSG 2262.
[6]	"A small protein that mediates the activation of a two-component system by another two-component system." Kox L.F.F., Woesten M.M.S.M., Groisman E.A. EMBO J. 19:1861-1872(2000) [PubMed: 10775270] [Abstract] Cited for: MUTAGENESIS OF ASP-51, PHOSPHORYLATION AT ASP-51. Strain: ATCC 14028s / SGSG 2262.
[7]	"Closing the loop: the PmrA/PmrB two-component system negatively controls expression of its posttranscriptional activator PmrD." Kato A., Latifi T., Groisman E.A. Proc. Natl. Acad. Sci. U.S.A. 100:4706-4711(2003) [PubMed: 12676988] [Abstract] Cited for: FUNCTION, REGULATION BY PMRD. Strain: ATCC 14028s / SGSG 2262.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L13395 Genomic DNA. Translation: AAA72365.1. AY725348 Genomic DNA. Translation: AAV92795.1. AE006468 Genomic DNA. Translation: AAL23116.1.
PIR	B40656.
3D structure databases
ProteinModelPortal	P36556.
ModBase	Search...
Proteomic databases
PRIDE	P36556.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus STM4292 in contig AE006468_GR.
KEGG	stm:STM4292.
PATRIC	32387493. VBISalEnt20916_4514.
Phylogenomic databases
HOGENOM	HBG753323.
OMA	NEPATNT.
ProtClustDB	PRK10643.
Enzyme and pathway databases
BioCyc	STYP99287:STM4292-MONOMER.
Family and domain databases
InterPro	IPR011006. CheY-like_superfamily. IPR001867. Sig_transdc_resp-reg_C. IPR001789. Sig_transdc_resp-reg_receiver. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07771.
Pfam	PF00072. Response_reg. 1 hit. PF00486. Trans_reg_C. 1 hit. [Graphical view]
SMART	SM00448. REC. 1 hit. SM00862. Trans_reg_C. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BASR_SALTY

Accession

Primary (citable) accession number: P36556
Secondary accession number(s): Q5MN92

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program