Putative multidrug resistance protein MdtD - Escherichia coli (strain K12)

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P36554 (MDTD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative multidrug resistance protein MdtD

Gene names

Name:	mdtD
Synonyms:	yegB
Ordered Locus Names:	b2077, JW2062

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_01577.
Induction	Transcriptionally regulated by BaeR. Ref.1 Ref.6
Sequence similarities	Belongs to the major facilitator superfamily. TCR/tet family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to plasma membrane Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 471

471

Putative multidrug resistance protein MdtD HAMAP-Rule MF_01577

PRO_0000173406

Regions

Topological domain

1 – 11

Periplasmic Potential

Transmembrane

12 – 32

Helical; Potential

Topological domain

33 – 48

Cytoplasmic Potential

Transmembrane

49 – 69

Helical; Potential

Topological domain

70 – 76

Periplasmic Potential

Transmembrane

77 – 97

Helical; Potential

Topological domain

98 – 101

Cytoplasmic Potential

Transmembrane

102 – 124

Helical; Potential

Topological domain

125 – 137

Periplasmic Potential

Transmembrane

138 – 158

Helical; Potential

Topological domain

159 – 164

Cytoplasmic Potential

Transmembrane

165 – 185

Helical; Potential

Topological domain

186 – 196

Periplasmic Potential

Transmembrane

197 – 217

Helical; Potential

Topological domain

218 – 224

Cytoplasmic Potential

Transmembrane

225 – 245

Helical; Potential

Topological domain

246 – 262

Periplasmic Potential

Transmembrane

263 – 283

Helical; Potential

Topological domain

284 – 285

Cytoplasmic Potential

Transmembrane

286 – 306

Helical; Potential

Topological domain

307 – 341

Periplasmic Potential

Transmembrane

342 – 362

Helical; Potential

Topological domain

363 – 395

Cytoplasmic Potential

Transmembrane

396 – 416

Helical; Potential

Topological domain

417 – 430

Periplasmic Potential

Transmembrane

431 – 451

Helical; Potential

Topological domain

452 – 471

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P36554 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: FCAF02AD330B5F42
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FASTA

471

50,901

        10         20         30         40         50         60 
MTDLPDSTRW QLWIVAFGFF MQSLDTTIVN TALPSMAQSL GESPLHMHMV IVSYVLTVAV 

        70         80         90        100        110        120 
MLPASGWLAD KVGVRNIFFT AIVLFTLGSL FCALSGTLNE LLLARALQGV GGAMMVPVGR 

       130        140        150        160        170        180 
LTVMKIVPRE QYMAAMTFVT LPGQVGPLLG PALGGLLVEY ASWHWIFLIN IPVGIIGAIA 

       190        200        210        220        230        240 
TLLLMPNYTM QTRRFDLSGF LLLAVGMAVL TLALDGSKGT GLSPLTIAGL VAVGVVALVL 

       250        260        270        280        290        300 
YLLHARNNNR ALFSLKLFRT RTFSLGLAGS FAGRIGSGML PFMTPVFLQI GLGFSPFHAG 

       310        320        330        340        350        360 
LMMIPMVLGS MGMKRIVVQV VNRFGYRRVL VATTLGLSLV TLLFMTTALL GWYYVLPFVL 

       370        380        390        400        410        420 
FLQGMVNSTR FSSMNTLTLK DLPDNLASSG NSLLSMIMQL SMSIGVTIAG LLLGLFGSQH 

       430        440        450        460        470 
VSVDSGTTQT VFMYTWLSMA LIIALPAFIF ARVPNDTHQN VAISRRKRSA Q

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC." Nagakubo S., Nishino K., Hirata T., Yamaguchi A. J. Bacteriol. 184:4161-4167(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Novel members of the two-component signal transduction genes in Escherichia coli." Nagasawa S., Ishige K., Mizuno T. J. Biochem. 114:350-357(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 381-471. Strain: K12.
[6]	"The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate." Baranova N., Nikaido H. J. Bacteriol. 184:4168-4176(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB089190 Genomic DNA. Translation: BAC06610.1. U00096 Genomic DNA. Translation: AAC75138.1. AP009048 Genomic DNA. Translation: BAA15933.1. D14054 Genomic DNA. Translation: BAA03139.1.
PIR	D64974.
RefSeq	NP_416581.1. NC_000913.2. YP_490319.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P36554.
SMR	P36554. Positions 27-180.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11876N.
IntAct	P36554. 1 interaction.
MINT	MINT-1320504.
STRING	511145.b2077.
Protein family/group databases
TCDB	2.A.1.3.26. major facilitator superfamily (MFS).
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75138; AAC75138; b2077. BAA15933; BAA15933; BAA15933.
GeneID	12931431. 946601.
KEGG	ecj:Y75_p2040. eco:b2077.
PATRIC	32119487. VBIEscCol129921_2154.
Organism-specific databases
EchoBASE	EB2057.
EcoGene	EG12136. mdtD.
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000193484.
OMA	HARHNER.
ProtClustDB	PRK10504.
Enzyme and pathway databases
BioCyc	EcoCyc:B2077-MONOMER. ECOL316407:JW2062-MONOMER.
Gene expression databases
Genevestigator	P36554.
Family and domain databases
HAMAP	MF_01577. MFS_MdtD.
InterPro	IPR004638. Drug-R_transpt_efflux_EmrB. IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR023721. Multi-R_MdtD_put. IPR001411. Tet-R_TetB/Drug-R_transptr. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
PRINTS	PR01036. TCRTETB.
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00711. efflux_EmrB. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDTD_ECOLI

Accession

Primary (citable) accession number: P36554
Secondary accession number(s): P76400, Q54A55

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents