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Protein

Oxygen-dependent coproporphyrinogen-III oxidase

Gene

hemF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.UniRule annotation2 Publications

Catalytic activityi

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.UniRule annotation2 Publications

Cofactori

Mn2+UniRule annotation1 Publication

Enzyme regulationi

Inhibited by protoporphyrinogen-IX, by metal chelator (EGTA) and by low concentrations of heavy metal ions.2 Publications

Kineticsi

Kcat is 0.17 min(-1) for decarboxylation of coproporphyrinogen-III (at pH 6 and at 37 degrees Celsius).1 Publication

  1. KM=2.6 µM for coproporphyrinogen-III (at pH 6 at 37 degrees Celsius)2 Publications
  2. KM=3.1 µM for coproporphyrinogen-III (at pH 7)2 Publications
  1. Vmax=1.3 µmol/min/mg enzyme (at pH 6 at 37 degrees Celsius)2 Publications

pH dependencei

Optimum pH is between 6 and 7.2 Publications

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Oxygen-dependent coproporphyrinogen-III oxidase (hemF)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921SubstrateUniRule annotation
Metal bindingi96 – 961ManganeseCurated
Active sitei106 – 1061Proton donorUniRule annotation
Metal bindingi106 – 1061ManganeseCurated
Metal bindingi145 – 1451ManganeseCurated
Metal bindingi175 – 1751ManganeseCurated
Sitei175 – 1751Important for dimerizationUniRule annotation

GO - Molecular functioni

  • coproporphyrinogen oxidase activity Source: EcoCyc
  • manganese ion binding Source: EcoCyc
  • protein homodimerization activity Source: UniProtKB
  • tetrapyrrole binding Source: EcoliWiki

GO - Biological processi

  • heme biosynthetic process Source: EcoliWiki
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:COPROGENOXI-MONOMER.
ECOL316407:JW2429-MONOMER.
MetaCyc:COPROGENOXI-MONOMER.
BRENDAi1.3.3.3. 2026.
UniPathwayiUPA00251; UER00322.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)
Short name:
CPOUniRule annotation
Short name:
Coprogen oxidaseUniRule annotation
Short name:
CoproporphyrinogenaseUniRule annotation
Gene namesi
Name:hemFUniRule annotation
Ordered Locus Names:b2436, JW2429
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12189. hemF.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi96 – 961H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication
Mutagenesisi106 – 1061H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication
Mutagenesisi145 – 1451H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication
Mutagenesisi175 – 1751H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication
Mutagenesisi274 – 2741W → L: Complete loss of oxidase activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Oxygen-dependent coproporphyrinogen-III oxidasePRO_0000109894Add
BLAST

Proteomic databases

PaxDbiP36553.
PRIDEiP36553.

Interactioni

Subunit structurei

Homodimer.UniRule annotation2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4260574. 13 interactions.
IntActiP36553. 3 interactions.
STRINGi511145.b2436.

Structurei

3D structure databases

ProteinModelPortaliP36553.
SMRiP36553. Positions 8-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni108 – 1103Substrate bindingUniRule annotation
Regioni240 – 27536Important for dimerizationUniRule annotationAdd
BLAST
Regioni258 – 2603Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DBS. Bacteria.
COG0408. LUCA.
HOGENOMiHOG000262768.
InParanoidiP36553.
KOiK00228.
OMAiIDLTPHY.
PhylomeDBiP36553.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas. 1 hit.
InterProiIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36553-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPDAHQVKQ FLLNLQDTIC QQLTAVDGAE FVEDSWQREA GGGGRSRVLR
60 70 80 90 100
NGGVFEQAGV NFSHVHGEAM PASATAHRPE LAGRSFEAMG VSLVVHPHNP
110 120 130 140 150
YVPTSHANVR FFIAEKPGAD PVWWFGGGFD LTPFYGFEED AIHWHRTARD
160 170 180 190 200
LCLPFGEDVY PRYKKWCDEY FYLKHRNEQR GIGGLFFDDL NTPDFDRCFA
210 220 230 240 250
FMQAVGKGYT DAYLPIVERR KAMAYGERER NFQLYRRGRY VEFNLVWDRG
260 270 280 290
TLFGLQTGGR TESILMSMPP LVRWEYDYQP KDGSPEAALS EFIKVRDWV
Length:299
Mass (Da):34,323
Last modified:June 1, 1994 - v1
Checksum:i53667E5C7B6D0198
GO

Mass spectrometryi

Molecular mass is 35390 Da from positions 1 - 299. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75413 Genomic DNA. Translation: CAA53167.1.
U00096 Genomic DNA. Translation: AAC75489.1.
AP009048 Genomic DNA. Translation: BAA16319.1.
PIRiB36964.
RefSeqiNP_416931.1. NC_000913.3.
WP_000801365.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75489; AAC75489; b2436.
BAA16319; BAA16319; BAA16319.
GeneIDi946908.
KEGGiecj:JW2429.
eco:b2436.
PATRICi32120257. VBIEscCol129921_2531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75413 Genomic DNA. Translation: CAA53167.1.
U00096 Genomic DNA. Translation: AAC75489.1.
AP009048 Genomic DNA. Translation: BAA16319.1.
PIRiB36964.
RefSeqiNP_416931.1. NC_000913.3.
WP_000801365.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP36553.
SMRiP36553. Positions 8-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260574. 13 interactions.
IntActiP36553. 3 interactions.
STRINGi511145.b2436.

Proteomic databases

PaxDbiP36553.
PRIDEiP36553.

Protocols and materials databases

DNASUi946908.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75489; AAC75489; b2436.
BAA16319; BAA16319; BAA16319.
GeneIDi946908.
KEGGiecj:JW2429.
eco:b2436.
PATRICi32120257. VBIEscCol129921_2531.

Organism-specific databases

EchoBASEiEB2106.
EcoGeneiEG12189. hemF.

Phylogenomic databases

eggNOGiENOG4105DBS. Bacteria.
COG0408. LUCA.
HOGENOMiHOG000262768.
InParanoidiP36553.
KOiK00228.
OMAiIDLTPHY.
PhylomeDBiP36553.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00322.
BioCyciEcoCyc:COPROGENOXI-MONOMER.
ECOL316407:JW2429-MONOMER.
MetaCyc:COPROGENOXI-MONOMER.
BRENDAi1.3.3.3. 2026.

Miscellaneous databases

PROiP36553.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas. 1 hit.
InterProiIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM6_ECOLI
AccessioniPrimary (citable) accession number: P36553
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.