Reviewed, UniProtKB/Swiss-Prot P36553 (HEM6_ECOLI)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, aerobic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	b2436, JW2429

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Cofactor	Manganese. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer. HAMAP MF_00333
Subcellular location	Cytoplasm. HAMAP MF_00333
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
htpG	P0A6Z3	1	EBI-1114572,EBI-369221
nadE	P18843	1	EBI-1114572,EBI-548960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Coproporphyrinogen-III oxidase, aerobic		PRO_0000109894
Sites
	Metal binding	96	1	Manganese HAMAP MF_00333
	Metal binding	106	1	Manganese HAMAP MF_00333
	Metal binding	145	1	Manganese HAMAP MF_00333
	Metal binding	175	1	Manganese HAMAP MF_00333

Sequences

	Sequence		Length	Mass (Da)
	P36553-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 53667E5C7B6D0198	FASTA	299	34,323
	10 20 30 40 50 60 MKPDAHQVKQ FLLNLQDTIC QQLTAVDGAE FVEDSWQREA GGGGRSRVLR NGGVFEQAGV 70 80 90 100 110 120 NFSHVHGEAM PASATAHRPE LAGRSFEAMG VSLVVHPHNP YVPTSHANVR FFIAEKPGAD 130 140 150 160 170 180 PVWWFGGGFD LTPFYGFEED AIHWHRTARD LCLPFGEDVY PRYKKWCDEY FYLKHRNEQR 190 200 210 220 230 240 GIGGLFFDDL NTPDFDRCFA FMQAVGKGYT DAYLPIVERR KAMAYGERER NFQLYRRGRY 250 260 270 280 290 VEFNLVWDRG TLFGLQTGGR TESILMSMPP LVRWEYDYQP KDGSPEAALS EFIKVRDWV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant." Troup B., Jahn M., Hungerer C., Jahn D. J. Bacteriol. 176:673-680(1994) [PubMed: 8300522] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Oxygen-dependent coproporphyrinogen-III oxidase from Escherichia coli: one-step purification and biochemical characterisation." Breckau D., Mahlitz E., Sauerwald A., Layer G., Jahn D. FEMS Microbiol. Lett. 226:31-37(2003) [PubMed: 13129604] [Abstract] Cited for: CHARACTERIZATION, CRYSTALLIZATION.
[6]	"Oxygen-dependent coproporphyrinogen III oxidase (HemF) from Escherichia coli is stimulated by manganese." Breckau D., Mahlitz E., Sauerwald A., Layer G., Jahn D. J. Biol. Chem. 278:46625-46631(2003) [PubMed: 12975365] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF TRP-36; HIS-96; HIS-106; TRP-123; TYR-135; HIS-145; TYR-160; TRP-166; CYS-167; TYR-170; HIS-175; TYR-213; TYR-240; TYR-276 AND TRP-298.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75413 Genomic DNA. Translation: CAA53167.1. U00096 Genomic DNA. Translation: AAC75489.1. AP009048 Genomic DNA. Translation: BAA16319.1.
PIR	B36964.
RefSeq	AP_003030.1. NP_416931.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P36553. 3 interactions.
Genome annotation databases
GeneID	946908.
GenomeReviews	Gene locus JW2429 in contig AP009048_GR. Gene locus b2436 in contig U00096_GR.
KEGG	ecj:JW2429. eco:b2436.
Organism-specific databases
EchoBASE	EB2106.
EcoGene	EG12189. hemF.
CMR	Search...
Phylogenomic databases
HOGENOM	P36553.
OMA	P36553. VKAYLLD.
Enzyme and pathway databases
BioCyc	EcoCyc:COPROGENOXI-MON. MetaCyc:COPROGENOXI-MON.
BRENDA	1.3.3.3. 246.
Family and domain databases
HAMAP	MF_00333. [Tree]
InterPro	IPR001260. Coprogen_oxidas. IPR018375. Coproporphyrinogen-3_ox_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_ECOLI

Accession

Primary (citable) accession number: P36553

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)