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Protein

Oxygen-dependent coproporphyrinogen-III oxidase

Gene

hemF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.UniRule annotation2 Publications

Catalytic activityi

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.UniRule annotation2 Publications

Cofactori

Mn2+UniRule annotation1 Publication

Enzyme regulationi

Inhibited by protoporphyrinogen-IX, by metal chelator (EGTA) and by low concentrations of heavy metal ions.2 Publications

Kineticsi

Kcat is 0.17 min(-1) for decarboxylation of coproporphyrinogen-III (at pH 6 and at 37 degrees Celsius).1 Publication
  1. KM=2.6 µM for coproporphyrinogen-III (at pH 6 at 37 degrees Celsius)2 Publications
  2. KM=3.1 µM for coproporphyrinogen-III (at pH 7)2 Publications
  1. Vmax=1.3 µmol/min/mg enzyme (at pH 6 at 37 degrees Celsius)2 Publications

pH dependencei

Optimum pH is between 6 and 7.2 Publications

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Oxygen-dependent coproporphyrinogen-III oxidase (hemF)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateUniRule annotation1
Metal bindingi96ManganeseCurated1
Active sitei106Proton donorUniRule annotation1
Metal bindingi106ManganeseCurated1
Metal bindingi145ManganeseCurated1
Metal bindingi175ManganeseCurated1
Sitei175Important for dimerizationUniRule annotation1

GO - Molecular functioni

  • coproporphyrinogen oxidase activity Source: EcoCyc
  • manganese ion binding Source: EcoCyc
  • protein homodimerization activity Source: UniProtKB
  • tetrapyrrole binding Source: EcoliWiki

GO - Biological processi

  • heme biosynthetic process Source: EcoliWiki
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:COPROGENOXI-MONOMER
MetaCyc:COPROGENOXI-MONOMER
BRENDAi1.3.3.3 2026
UniPathwayiUPA00251; UER00322

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)
Short name:
CPOUniRule annotation
Short name:
Coprogen oxidaseUniRule annotation
Short name:
CoproporphyrinogenaseUniRule annotation
Gene namesi
Name:hemFUniRule annotation
Ordered Locus Names:b2436, JW2429
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12189 hemF

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi96H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication1
Mutagenesisi106H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication1
Mutagenesisi145H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication1
Mutagenesisi175H → L: Complete loss of oxidase activity and absence of manganese ions. 1 Publication1
Mutagenesisi274W → L: Complete loss of oxidase activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098941 – 299Oxygen-dependent coproporphyrinogen-III oxidaseAdd BLAST299

Proteomic databases

PaxDbiP36553
PRIDEiP36553

Interactioni

Subunit structurei

Homodimer.UniRule annotation2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4260574, 13 interactors
IntActiP36553, 3 interactors
STRINGi316385.ECDH10B_2601

Structurei

3D structure databases

ProteinModelPortaliP36553
SMRiP36553
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 110Substrate bindingUniRule annotation3
Regioni240 – 275Important for dimerizationUniRule annotationAdd BLAST36
Regioni258 – 260Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DBS Bacteria
COG0408 LUCA
HOGENOMiHOG000262768
InParanoidiP36553
KOiK00228
OMAiMDLTPYY
PhylomeDBiP36553

Family and domain databases

Gene3Di3.40.1500.10, 1 hit
HAMAPiMF_00333 Coprogen_oxidas, 1 hit
InterProiView protein in InterPro
IPR001260 Coprogen_oxidase_aer
IPR036406 Coprogen_oxidase_aer_sf
IPR018375 Coprogen_oxidase_CS
PANTHERiPTHR10755 PTHR10755, 1 hit
PfamiView protein in Pfam
PF01218 Coprogen_oxidas, 1 hit
PIRSFiPIRSF000166 Coproporphyri_ox, 1 hit
PRINTSiPR00073 COPRGNOXDASE
SUPFAMiSSF102886 SSF102886, 1 hit
PROSITEiView protein in PROSITE
PS01021 COPROGEN_OXIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

P36553-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPDAHQVKQ FLLNLQDTIC QQLTAVDGAE FVEDSWQREA GGGGRSRVLR
60 70 80 90 100
NGGVFEQAGV NFSHVHGEAM PASATAHRPE LAGRSFEAMG VSLVVHPHNP
110 120 130 140 150
YVPTSHANVR FFIAEKPGAD PVWWFGGGFD LTPFYGFEED AIHWHRTARD
160 170 180 190 200
LCLPFGEDVY PRYKKWCDEY FYLKHRNEQR GIGGLFFDDL NTPDFDRCFA
210 220 230 240 250
FMQAVGKGYT DAYLPIVERR KAMAYGERER NFQLYRRGRY VEFNLVWDRG
260 270 280 290
TLFGLQTGGR TESILMSMPP LVRWEYDYQP KDGSPEAALS EFIKVRDWV
Length:299
Mass (Da):34,323
Last modified:June 1, 1994 - v1
Checksum:i53667E5C7B6D0198
GO

Mass spectrometryi

Molecular mass is 35390 Da from positions 1 - 299. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75413 Genomic DNA Translation: CAA53167.1
U00096 Genomic DNA Translation: AAC75489.1
AP009048 Genomic DNA Translation: BAA16319.1
PIRiB36964
RefSeqiNP_416931.1, NC_000913.3
WP_000801365.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75489; AAC75489; b2436
BAA16319; BAA16319; BAA16319
GeneIDi946908
KEGGiecj:JW2429
eco:b2436
PATRICifig|1411691.4.peg.4295

Similar proteinsi

Entry informationi

Entry nameiHEM6_ECOLI
AccessioniPrimary (citable) accession number: P36553
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health