P36550 (CWLL_BACLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmuramoyl-L-alanine amidase CwlL
EC=3.5.1.28

Alternative name(s):
Autolysin
Cell wall hydrolase

Gene names

Name:

cwlL

Organism

Bacillus licheniformis

Taxonomic identifier

1402 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological_process	peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	By similarity
	Chain	40 – 360	321	N-acetylmuramoyl-L-alanine amidase CwlL		PRO_0000006455
Regions
	Repeat	166 – 191	26	1-1
	Repeat	196 – 259	64	2-1
	Repeat	265 – 289	25	1-2
	Repeat	291 – 355	65	2-2
	Region	166 – 289	124	2 X approximate repeats
	Region	196 – 355	160	2 X approximate repeats

Sequences

	Sequence		Length	Mass (Da)
	P36550 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 37C07B4A084364DA	FASTA	360	38,996
	10 20 30 40 50 60 MVKVVKNFVK VNQYTRPGLK LAGVKGIVMH YTATPGASAL NERDYFNGTC IAIKRKASSA 70 80 90 100 110 120 HYFVDRKEAQ HIIPENEVAY HAHDKNRCYV SFLKPNANTK SISVEMCVEK DGMIHSETVQ 130 140 150 160 170 180 NAAELVADLC KRYGLSTNKI VRHYDVTNKI CPAPWVSDSS QLTTFRKKVD SLLGNKTVSK 190 200 210 220 230 240 TTSSTSQSSK STGTILKKGA SGSQVKALQK RLIAAGFSLP KYGADGSYEN ETVQAVKALQ 250 260 270 280 290 300 KKAGIAVDGI YGPATEKALA AIGAKKKKPS SNGKKTSYPL PSGIYKVKSP LMKGTGVRQI 310 320 330 340 350 360 QEALAALYFY PDKGAKNNGI DGYYGPKTAN AVKRFQLMHG LSADGIYGSD TKAKLKTLLK

References

[1]	"Molecular cloning, sequence analysis, and characterization of a new cell wall hydrolase, CwlL, of Bacillus licheniformis." Oda Y., Nakayama R., Kuroda A., Sekiguchi J. Mol. Gen. Genet. 241:380-388(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FD0120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13377 Genomic DNA. Translation: BAA02647.1.
PIR	S39916.
3D structure databases
ProteinModelPortal	P36550.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.101.10. 2 hits. 3.40.80.10. 1 hit.
InterPro	IPR002502. Amidase_domain. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Pfam	PF01510. Amidase_2. 1 hit. PF01471. PG_binding_1. 2 hits. [Graphical view]
SMART	SM00644. Ami_2. 1 hit. [Graphical view]
SUPFAM	SSF55846. Amidase_2. 1 hit. SSF47090. PGBD_like. 2 hits.
ProtoNet	Search...

Entry information

Entry name

CWLL_BACLI

Accession

Primary (citable) accession number: P36550

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program