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Protein

N-acetylmuramoyl-L-alanine amidase AmiA

Gene

amiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors.2 Publications

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

GO - Molecular functioni

  • N-acetylmuramoyl-L-alanine amidase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:NACMURLALAAMI1-MONOMER
MetaCyc:NACMURLALAAMI1-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase AmiA (EC:3.5.1.28)
Gene namesi
Name:amiA
Synonyms:yfeE
Ordered Locus Names:b2435, JW2428
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG11823 amiA

Subcellular locationi

  • Periplasm 1 Publication
  • Note: Distributed throughout the periplasm in all cells.

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoliWiki

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Mutants are growing in chains of 3 to 6 cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Tat-type signalPROSITE-ProRule annotationAdd BLAST34
ChainiPRO_000000646035 – 289N-acetylmuramoyl-L-alanine amidase AmiAAdd BLAST255

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP36548
PRIDEiP36548

Interactioni

Protein-protein interaction databases

BioGridi4259617, 388 interactors
DIPiDIP-9098N
IntActiP36548, 9 interactors
STRINGi316385.ECDH10B_2600

Structurei

3D structure databases

ProteinModelPortaliP36548
SMRiP36548
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108KNB Bacteria
COG0860 LUCA
HOGENOMiHOG000263827
InParanoidiP36548
KOiK01448
OMAiQIRPVHH
PhylomeDBiP36548

Family and domain databases

CDDicd02696 MurNAc-LAA, 1 hit
InterProiView protein in InterPro
IPR002508 MurNAc-LAA_cat
IPR006311 TAT_signal
PfamiView protein in Pfam
PF01520 Amidase_3, 1 hit
SMARTiView protein in SMART
SM00646 Ami_3, 1 hit
PROSITEiView protein in PROSITE
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTFKPLKTL TSRRQVLKAG LAALTLSGMS QAIAKDELLK TSNGHSKPKA
60 70 80 90 100
KKSGGKRVVV LDPGHGGIDT GAIGRNGSKE KHVVLAIAKN VRSILRNHGI
110 120 130 140 150
DARLTRSGDT FIPLYDRVEI AHKHGADLFM SIHADGFTNP KAAGASVFAL
160 170 180 190 200
SNRGASSAMA KYLSERENRA DEVAGKKATD KDHLLQQVLF DLVQTDTIKN
210 220 230 240 250
SLTLGSHILK KIKPVHKLHS RNTEQAAFVV LKSPSVPSVL VETSFITNPE
260 270 280
EERLLGTAAF RQKIATAIAE GVISYFHWFD NQKAHSKKR
Length:289
Mass (Da):31,412
Last modified:June 1, 1994 - v1
Checksum:i614D1E526D9FEFC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75413 Genomic DNA Translation: CAA53166.1
U00096 Genomic DNA Translation: AAC75488.1
AP009048 Genomic DNA Translation: BAA16318.1
X63986 Genomic DNA Translation: CAB57860.1
PIRiA36964
RefSeqiNP_416930.1, NC_000913.3
WP_000102886.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75488; AAC75488; b2435
BAA16318; BAA16318; BAA16318
GeneIDi946916
KEGGiecj:JW2428
eco:b2435
PATRICifig|1411691.4.peg.4296

Entry informationi

Entry nameiAMIA_ECOLI
AccessioniPrimary (citable) accession number: P36548
Secondary accession number(s): P78199
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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