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P36548

- AMIA_ECOLI

UniProt

P36548 - AMIA_ECOLI

Protein

N-acetylmuramoyl-L-alanine amidase AmiA

Gene

amiA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors.2 Publications

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    GO - Molecular functioni

    1. N-acetylmuramoyl-L-alanine amidase activity Source: EcoliWiki

    GO - Biological processi

    1. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciEcoCyc:NACMURLALAAMI1-MONOMER.
    ECOL316407:JW2428-MONOMER.
    MetaCyc:NACMURLALAAMI1-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylmuramoyl-L-alanine amidase AmiA (EC:3.5.1.28)
    Gene namesi
    Name:amiA
    Synonyms:yfeE
    Ordered Locus Names:b2435, JW2428
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11823. amiA.

    Subcellular locationi

    Periplasm 1 Publication
    Note: Distributed throughout the periplasm in all cells.

    GO - Cellular componenti

    1. periplasmic space Source: EcoliWiki

    Keywords - Cellular componenti

    Periplasm

    Pathology & Biotechi

    Disruption phenotypei

    Mutants are growing in chains of 3 to 6 cells.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Tat-type signalPROSITE-ProRule annotationAdd
    BLAST
    Chaini35 – 289255N-acetylmuramoyl-L-alanine amidase AmiAPRO_0000006460Add
    BLAST

    Post-translational modificationi

    Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.

    Proteomic databases

    PaxDbiP36548.
    PRIDEiP36548.

    Expressioni

    Gene expression databases

    GenevestigatoriP36548.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-9098N.
    IntActiP36548. 9 interactions.
    STRINGi511145.b2435.

    Structurei

    3D structure databases

    ProteinModelPortaliP36548.
    SMRiP36548. Positions 59-272.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0860.
    HOGENOMiHOG000263827.
    KOiK01448.
    OMAiSYFNWFD.
    OrthoDBiEOG6CP3X3.
    PhylomeDBiP36548.

    Family and domain databases

    Gene3Di3.40.630.40. 2 hits.
    InterProiIPR002508. CW_Hdrlase/autolysin_cat.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF01520. Amidase_3. 1 hit.
    [Graphical view]
    SMARTiSM00646. Ami_3. 1 hit.
    [Graphical view]
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36548-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTFKPLKTL TSRRQVLKAG LAALTLSGMS QAIAKDELLK TSNGHSKPKA    50
    KKSGGKRVVV LDPGHGGIDT GAIGRNGSKE KHVVLAIAKN VRSILRNHGI 100
    DARLTRSGDT FIPLYDRVEI AHKHGADLFM SIHADGFTNP KAAGASVFAL 150
    SNRGASSAMA KYLSERENRA DEVAGKKATD KDHLLQQVLF DLVQTDTIKN 200
    SLTLGSHILK KIKPVHKLHS RNTEQAAFVV LKSPSVPSVL VETSFITNPE 250
    EERLLGTAAF RQKIATAIAE GVISYFHWFD NQKAHSKKR 289
    Length:289
    Mass (Da):31,412
    Last modified:June 1, 1994 - v1
    Checksum:i614D1E526D9FEFC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75413 Genomic DNA. Translation: CAA53166.1.
    U00096 Genomic DNA. Translation: AAC75488.1.
    AP009048 Genomic DNA. Translation: BAA16318.1.
    X63986 Genomic DNA. Translation: CAB57860.1.
    PIRiA36964.
    RefSeqiNP_416930.1. NC_000913.3.
    YP_490671.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75488; AAC75488; b2435.
    BAA16318; BAA16318; BAA16318.
    GeneIDi12930623.
    946916.
    KEGGiecj:Y75_p2396.
    eco:b2435.
    PATRICi32120255. VBIEscCol129921_2530.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75413 Genomic DNA. Translation: CAA53166.1 .
    U00096 Genomic DNA. Translation: AAC75488.1 .
    AP009048 Genomic DNA. Translation: BAA16318.1 .
    X63986 Genomic DNA. Translation: CAB57860.1 .
    PIRi A36964.
    RefSeqi NP_416930.1. NC_000913.3.
    YP_490671.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P36548.
    SMRi P36548. Positions 59-272.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9098N.
    IntActi P36548. 9 interactions.
    STRINGi 511145.b2435.

    Proteomic databases

    PaxDbi P36548.
    PRIDEi P36548.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75488 ; AAC75488 ; b2435 .
    BAA16318 ; BAA16318 ; BAA16318 .
    GeneIDi 12930623.
    946916.
    KEGGi ecj:Y75_p2396.
    eco:b2435.
    PATRICi 32120255. VBIEscCol129921_2530.

    Organism-specific databases

    EchoBASEi EB1770.
    EcoGenei EG11823. amiA.

    Phylogenomic databases

    eggNOGi COG0860.
    HOGENOMi HOG000263827.
    KOi K01448.
    OMAi SYFNWFD.
    OrthoDBi EOG6CP3X3.
    PhylomeDBi P36548.

    Enzyme and pathway databases

    BioCyci EcoCyc:NACMURLALAAMI1-MONOMER.
    ECOL316407:JW2428-MONOMER.
    MetaCyc:NACMURLALAAMI1-MONOMER.

    Miscellaneous databases

    PROi P36548.

    Gene expression databases

    Genevestigatori P36548.

    Family and domain databases

    Gene3Di 3.40.630.40. 2 hits.
    InterProi IPR002508. CW_Hdrlase/autolysin_cat.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF01520. Amidase_3. 1 hit.
    [Graphical view ]
    SMARTi SM00646. Ami_3. 1 hit.
    [Graphical view ]
    PROSITEi PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant."
      Troup B., Jahn M., Hungerer C., Jahn D.
      J. Bacteriol. 176:673-680(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance."
      Tanaka K., Muramatsu S., Yamada H., Mizuno T.
      Mol. Gen. Genet. 226:367-376(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
    6. "Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli."
      Heidrich C., Templin M.F., Ursinus A., Merdanovic M., Berger J., Schwarz H., de Pedro M.A., Holtje J.V.
      Mol. Microbiol. 41:167-178(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN AMIDASE, DISRUPTION PHENOTYPE.
      Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.
    7. "The Escherichia coli amidase AmiC is a periplasmic septal ring component exported via the twin-arginine transport pathway."
      Bernhardt T.G., de Boer P.A.
      Mol. Microbiol. 48:1171-1182(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, EXPORT VIA THE TAT-SYSTEM.
      Strain: K12.
    8. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
      Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
      J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: EXPORT VIA THE TAT-SYSTEM AND THE SEC-SYSTEM.
    9. "Growth of Escherichia coli: significance of peptidoglycan degradation during elongation and septation."
      Uehara T., Park J.T.
      J. Bacteriol. 190:3914-3922(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12.

    Entry informationi

    Entry nameiAMIA_ECOLI
    AccessioniPrimary (citable) accession number: P36548
    Secondary accession number(s): P78199
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3