Reviewed, UniProtKB/Swiss-Prot P36548 (AMIA_ECOLI)

Names and origin

Protein names

Recommended name:
    Probable N-acetylmuramoyl-L-alanine amidase amiA
    EC=3.5.1.28

Gene names

Name:	amiA
Synonyms:	yfeE
Ordered Locus Names:	b2435, JW2428

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Post-translational modification	Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from genetic interaction. Source: UniProtKB peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell periplasmic space Inferred from direct assay. Source: UniProtKB
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Tat-type signal Potential
	Chain	35 – 289	255	Probable N-acetylmuramoyl-L-alanine amidase amiA		PRO_0000006460

Sequences

	Sequence		Length	Mass (Da)
	P36548-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 614D1E526D9FEFC5	FASTA	289	31,412
	10 20 30 40 50 60 MSTFKPLKTL TSRRQVLKAG LAALTLSGMS QAIAKDELLK TSNGHSKPKA KKSGGKRVVV 70 80 90 100 110 120 LDPGHGGIDT GAIGRNGSKE KHVVLAIAKN VRSILRNHGI DARLTRSGDT FIPLYDRVEI 130 140 150 160 170 180 AHKHGADLFM SIHADGFTNP KAAGASVFAL SNRGASSAMA KYLSERENRA DEVAGKKATD 190 200 210 220 230 240 KDHLLQQVLF DLVQTDTIKN SLTLGSHILK KIKPVHKLHS RNTEQAAFVV LKSPSVPSVL 250 260 270 280 VETSFITNPE EERLLGTAAF RQKIATAIAE GVISYFHWFD NQKAHSKKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant." Troup B., Jahn M., Hungerer C., Jahn D. J. Bacteriol. 176:673-680(1994) [PubMed: 8300522] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance." Tanaka K., Muramatsu S., Yamada H., Mizuno T. Mol. Gen. Genet. 226:367-376(1991) [PubMed: 1903834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
[6]	"Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G. J. Biol. Chem. 282:8309-8316(2007) [PubMed: 17218314] [Abstract] Cited for: EXPORT VIA THE TAT-SYSTEM AND THE SEC-SYSTEM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75413 Genomic DNA. Translation: CAA53166.1. U00096 Genomic DNA. Translation: AAC75488.1. AP009048 Genomic DNA. Translation: BAA16318.1. X63986 Genomic DNA. Translation: CAB57860.1.
PIR	A36964.
RefSeq	AP_003029.1. NP_416930.1.
3D structure databases
SMR	P36548. Positions 57-278.
ModBase	Search...
Protein-protein interaction databases
STRING	P36548.
Genome annotation databases
GeneID	946916.
GenomeReviews	Gene locus JW2428 in contig AP009048_GR. Gene locus b2435 in contig U00096_GR.
KEGG	ecj:JW2428. eco:b2435.
Organism-specific databases
EchoBASE	EB1770.
EcoGene	EG11823. amiA.
CMR	Search...
Phylogenomic databases
eggNOG	COG0860.
HOGENOM	HBG656726.
OMA	DRENRAD.
Enzyme and pathway databases
BioCyc	EcoCyc:NACMURLALAAMI1-MONOMER. ECOL168927:B2435-MONOMER.
Gene expression databases
Genevestigator	P36548.
Family and domain databases
InterPro	IPR002508. CW_Hdrlase/autolysin_cat. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:3.40.630.40. Cell_wall_OHase/autolysin_cat. 1 hit.
Pfam	PF01520. Amidase_3. 1 hit. [Graphical view]
SMART	SM00646. Ami_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMIA_ECOLI

Accession

Primary (citable) accession number: P36548
Secondary accession number(s): P78199

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)