Reviewed, UniProtKB/Swiss-Prot P36547 (EUTR_ECOLI)

Names and origin

Protein names

Recommended name:
    HTH-type transcriptional regulator eutR
Alternative name(s):
    Ethanolamine operon regulatory protein

Gene names

Name:	eutR
Synonyms:	yfeG
Ordered Locus Names:	b2437, JW2430

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Activates the transcription of the eut operon. Also positively regulates its own transcription. Probably binds ethanolamine and vitamin B12 as effectors By similarity.
Sequence similarities	Contains 1 HTH araC/xylS-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	HTH-type transcriptional regulator eutR		PRO_0000194509
Regions
	DNA binding	259 – 278	20	H-T-H motif By similarity
Experimental info
	Sequence conflict	276	1	A → R in CAA53168. Ref.1
	Sequence conflict	285	1	P → R in CAA53168. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36547-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 1281A43DC31DE845	FASTA	350	40,160
	10 20 30 40 50 60 MKKTRTANLH HLYHEPLPEN LKLTPKVEVD NVHQRQTTDV YEHALTITAW QQIYDQLHPG 70 80 90 100 110 120 KFHGEFTEIL LDDIQVFREY TGLALRQSCL VWPNSFWFGI PATRGEQGFI GSQCLGSAEI 130 140 150 160 170 180 ATRPGGTEFE LSTPDDYTIL GVVLSEDVIT RQANFLHNPD RVLHMLRNQS ALEVKEQHKA 190 200 210 220 230 240 ALWGFVQQAL ATFCENPENL HQPAVRKVLG DNLLMAMGAM LEEAQPMVTA ESISHQSYRR 250 260 270 280 290 300 LLSRAREYVL ENMSEPVTVL DLCNQLHVSR RTLQNAFHAI LGIGPNAWLK RIRLNAVRRE 310 320 330 340 350 LISPWSQSMT VKDAAMQWGF WHLGQFATDY QQLFSEKPSL TLHQRMREWG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant." Troup B., Jahn M., Hungerer C., Jahn D. J. Bacteriol. 176:673-680(1994) [PubMed: 8300522] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75413 Genomic DNA. Translation: CAA53168.1. U00096 Genomic DNA. Translation: AAC75490.1. AP009048 Genomic DNA. Translation: BAA16320.1.
PIR	D65018.
RefSeq	AP_003031.1. NP_416932.1.
3D structure databases
SMR	P36547. Positions 241-341.
ModBase	Search...
Protein-protein interaction databases
STRING	P36547.
Protein family/group databases
AraC-XylS	Search...
Genome annotation databases
GeneID	946911.
GenomeReviews	Gene locus JW2430 in contig AP009048_GR. Gene locus b2437 in contig U00096_GR.
KEGG	ecj:JW2430. eco:b2437.
Organism-specific databases
EchoBASE	EB2107.
EcoGene	EG12190. eutR.
CMR	Search...
Phylogenomic databases
eggNOG	COG2207.
HOGENOM	HBG367418.
OMA	RRWIVEQ.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12190-MONOMER. ECOL168927:B2437-MONOMER.
Gene expression databases
Genevestigator	P36547.
Family and domain databases
InterPro	IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR000005. HTH_AraC-typ. IPR018062. HTH_AraC-typ_sub_2. IPR018060. HTH_AraC_domain. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00165. HTH_AraC. 2 hits. [Graphical view]
SMART	SM00342. HTH_ARAC. 1 hit. [Graphical view]
PROSITE	PS00041. HTH_ARAC_FAMILY_1. 1 hit. PS01124. HTH_ARAC_FAMILY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EUTR_ECOLI

Accession

Primary (citable) accession number: P36547
Secondary accession number(s): P77451

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)