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Protein

Neuronal acetylcholine receptor subunit alpha-7

Gene

CHRNA7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

GO - Molecular functioni

  • acetylcholine binding Source: UniProtKB
  • acetylcholine-gated cation-selective channel activity Source: UniProtKB
  • acetylcholine receptor activity Source: UniProtKB
  • amyloid-beta binding Source: UniProtKB
  • calcium channel activity Source: ARUK-UCL
  • chloride channel regulator activity Source: UniProtKB
  • ion channel activity Source: ARUK-UCL
  • ligand-gated ion channel activity Source: Reactome
  • protein homodimerization activity Source: UniProtKB
  • toxic substance binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
SignaLinkiP36544
SIGNORiP36544

Protein family/group databases

TCDBi1.A.9.1.7 the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-7
Gene namesi
Name:CHRNA7
Synonyms:NACHRA7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000175344.16
HGNCiHGNC:1960 CHRNA7
MIMi118511 gene
neXtProtiNX_P36544

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 230ExtracellularSequence analysisAdd BLAST208
Transmembranei231 – 255HelicalSequence analysisAdd BLAST25
Transmembranei262 – 280HelicalSequence analysisAdd BLAST19
Transmembranei296 – 317HelicalSequence analysisAdd BLAST22
Topological domaini318 – 469CytoplasmicSequence analysisAdd BLAST152
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi139Q → S: 115-fold more potently inhibited by the alpha-conotoxin ImI; but no change in inhibition by the alpha-conotoxin ImII. 1 Publication1

Organism-specific databases

DisGeNETi1139
89832
MalaCardsiCHRNA7
OpenTargetsiENSG00000175344
Orphaneti199318 15q13.3 microdeletion syndrome
PharmGKBiPA114
PA26483

Chemistry databases

ChEMBLiCHEMBL2492
DrugBankiDB01351 Amobarbital
DB01352 Aprobarbital
DB01483 Barbital
DB01496 Barbituric acid derivative
DB00237 Butabarbital
DB00241 Butalbital
DB01353 Butethal
DB09028 Cytisine
DB00514 Dextromethorphan
DB00898 Ethanol
DB00674 Galantamine
DB05708 GTS-21
DB01354 Heptabarbital
DB01355 Hexobarbital
DB00463 Metharbital
DB00849 Methylphenobarbital
DB00184 Nicotine
DB00312 Pentobarbital
DB01174 Phenobarbital
DB00794 Primidone
DB05740 RPI-78M
DB00418 Secobarbital
DB00306 Talbutal
DB00599 Thiopental
DB01273 Varenicline
GuidetoPHARMACOLOGYi468

Polymorphism and mutation databases

BioMutaiCHRNA7
DMDMi2506127

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000000036623 – 502Neuronal acetylcholine receptor subunit alpha-7Add BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi133N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi150 ↔ 164By similarity
Disulfide bondi212 ↔ 213Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PeptideAtlasiP36544
PRIDEiP36544
ProteomicsDBi55211
55212 [P36544-2]

PTM databases

iPTMnetiP36544
PhosphoSitePlusiP36544
SwissPalmiP36544

Expressioni

Gene expression databases

BgeeiENSG00000175344
CleanExiHS_CHRNA7
ExpressionAtlasiP36544 baseline and differential
GenevisibleiP36544 HS

Organism-specific databases

HPAiCAB033624
HPA029422

Interactioni

Subunit structurei

Homopentamer (By similarity). Interacts with RIC3; which is required for proper folding and assembly (PubMed:15504725, PubMed:16120769). Interacts with LYPD6 (PubMed:27344019). Interacts with the alpha-conotoxin RgIA (By similarity). Interacts with alpha-conotoxins ImI and ImII (PubMed:15609996).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SLURP1P550002EBI-79333,EBI-8830896

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi107561, 3 interactors
ComplexPortaliCPX-236 Neuronal nicotinic acetylcholine receptor complex, alpha7
CPX-240 Neuronal nicotinic acetylcholine receptor complex, alpha7-beta2
IntActiP36544, 6 interactors

Chemistry databases

BindingDBiP36544

Structurei

Secondary structure

1502
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 34Combined sources11
Beta strandi52 – 66Combined sources15
Turni67 – 70Combined sources4
Beta strandi78 – 83Combined sources6
Helixi85 – 87Combined sources3
Turni91 – 93Combined sources3
Beta strandi94 – 96Combined sources3
Beta strandi131 – 133Combined sources3
Beta strandi165 – 171Combined sources7
Beta strandi178 – 182Combined sources5
Beta strandi198 – 200Combined sources3
Beta strandi205 – 208Combined sources4
Beta strandi217 – 225Combined sources9
Helixi229 – 253Combined sources25
Helixi260 – 282Combined sources23
Beta strandi286 – 288Combined sources3
Helixi293 – 317Combined sources25
Beta strandi324 – 326Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MAWNMR-A228-326[»]
A467-495[»]
5AFHX-ray2.40A/B/C/D/E23-227[»]
5AFJX-ray2.20A/B/C/D/E23-227[»]
5AFKX-ray2.38A/B/C/D/E23-227[»]
5AFLX-ray2.38A/B/C/D/E23-227[»]
5AFMX-ray2.85A/B/C/D/E23-227[»]
5AFNX-ray2.15A/B/C/D/E23-227[»]
ProteinModelPortaliP36544
SMRiP36544
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00910000144023
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiP36544
KOiK04809
OMAiEAICNEW
OrthoDBiEOG091G0R20
PhylomeDBiP36544
TreeFamiTF315605

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P36544-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRCSPGGVWL ALAASLLHVS LQGEFQRKLY KELVKNYNPL ERPVANDSQP
60 70 80 90 100
LTVYFSLSLL QIMDVDEKNQ VLTTNIWLQM SWTDHYLQWN VSEYPGVKTV
110 120 130 140 150
RFPDGQIWKP DILLYNSADE RFDATFHTNV LVNSSGHCQY LPPGIFKSSC
160 170 180 190 200
YIDVRWFPFD VQHCKLKFGS WSYGGWSLDL QMQEADISGY IPNGEWDLVG
210 220 230 240 250
IPGKRSERFY ECCKEPYPDV TFTVTMRRRT LYYGLNLLIP CVLISALALL
260 270 280 290 300
VFLLPADSGE KISLGITVLL SLTVFMLLVA EIMPATSDSV PLIAQYFAST
310 320 330 340 350
MIIVGLSVVV TVIVLQYHHH DPDGGKMPKW TRVILLNWCA WFLRMKRPGE
360 370 380 390 400
DKVRPACQHK QRRCSLASVE MSAVAPPPAS NGNLLYIGFR GLDGVHCVPT
410 420 430 440 450
PDSGVVCGRM ACSPTHDEHL LHGGQPPEGD PDLAKILEEV RYIANRFRCQ
460 470 480 490 500
DESEAVCSEW KFAACVVDRL CLMAFSVFTI ICTIGILMSA PNFVEAVSKD

FA
Length:502
Mass (Da):56,449
Last modified:November 1, 1997 - v5
Checksum:iD94B3A482EAA0E42
GO
Isoform 2 (identifier: P36544-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-18: H → HGKATASPPSTPPWDPGHIPGASVRPAPGP

Note: No experimental confirmation available.
Show »
Length:531
Mass (Da):59,235
Checksum:i824BAFD78601D1FD
GO
Isoform 3 (identifier: P36544-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     81-102: SWTDHYLQWNVSEYPGVKTVRF → AYSRVPATSMYAGFPLMCSTAN
     103-502: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:102
Mass (Da):11,485
Checksum:i7A5F2F181F009A9E
GO

Sequence cautioni

The sequence AAH37571 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11A → G in CAA49778 (PubMed:8145738).Curated1
Sequence conflicti58S → N in AAA83561 (Ref. 2) Curated1
Sequence conflicti58S → N in AAK68111 (Ref. 6) Curated1
Sequence conflicti134S → P in AAA83561 (Ref. 2) Curated1
Sequence conflicti134S → P in AAK68111 (Ref. 6) Curated1
Sequence conflicti364C → S in CAA80672 (PubMed:8188270).Curated1
Sequence conflicti375A → G in CAA49778 (PubMed:8145738).Curated1
Sequence conflicti409 – 413RMACS → AWPAP in CAA80672 (PubMed:8188270).Curated5

Mass spectrometryi

Molecular mass is 54157.68 Da from positions 23 - 502. Determined by MALDI. 1 Publication

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04301918H → HGKATASPPSTPPWDPGHIP GASVRPAPGP in isoform 2. 1 Publication1
Alternative sequenceiVSP_05810781 – 102SWTDH…KTVRF → AYSRVPATSMYAGFPLMCST AN in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_058108103 – 502Missing in isoform 3. 1 PublicationAdd BLAST400

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70297 mRNA Translation: CAA49778.1
U40583 mRNA Translation: AAA83561.1
U62436 mRNA Translation: AAB40114.1
Y08420 mRNA Translation: CAA69697.1
AF385585 mRNA Translation: AAK68111.1
AK292069 mRNA Translation: BAF84758.1
AK294229 mRNA Translation: BAG57531.1
AC004460 Genomic DNA No translation available.
AC009562 Genomic DNA No translation available.
AC012236 Genomic DNA No translation available.
AC021316 Genomic DNA No translation available.
AC026150 Genomic DNA No translation available.
AC026951 Genomic DNA No translation available.
AC058803 Genomic DNA No translation available.
AC068448 Genomic DNA No translation available.
AC079969 Genomic DNA No translation available.
AC087481 Genomic DNA No translation available.
AC090829 Genomic DNA No translation available.
AC091057 Genomic DNA No translation available.
AC104266 Genomic DNA No translation available.
AC104759 Genomic DNA No translation available.
BC037571 mRNA Translation: AAH37571.1 Sequence problems.
BC101345 mRNA Translation: AAI01346.1
L25827 mRNA No translation available.
Z23141 mRNA Translation: CAA80672.1
AF332758 Genomic DNA Translation: AAK19515.1
CCDSiCCDS10027.1 [P36544-1]
CCDS53924.1 [P36544-2]
PIRiG02259
I37185 ACHUA7
RefSeqiNP_000737.1, NM_000746.5 [P36544-1]
NP_001177384.1, NM_001190455.2 [P36544-2]
NP_683709.1, NM_148911.1
XP_005254807.1, XM_005254750.2
XP_016877373.1, XM_017021884.1
UniGeneiHs.510853
Hs.511772
Hs.713151

Genome annotation databases

EnsembliENST00000306901; ENSP00000303727; ENSG00000175344 [P36544-1]
ENST00000437966; ENSP00000399087; ENSG00000175344 [P36544-3]
ENST00000454250; ENSP00000407546; ENSG00000175344 [P36544-2]
GeneIDi1139
89832
KEGGihsa:1139
UCSCiuc001zft.5 human [P36544-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACHA7_HUMAN
AccessioniPrimary (citable) accession number: P36544
Secondary accession number(s): A8K7Q4
, B4DFS0, Q15826, Q8IUZ4, Q96RH2, Q99555, Q9BXH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 192 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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