Reviewed, UniProtKB/Swiss-Prot P36544 (ACHA7_HUMAN)

Names and origin

Protein names

Recommended name:
    Neuronal acetylcholine receptor subunit alpha-7

Gene names

Name:	CHRNA7
Synonyms:	NACHRA7

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Subunit structure	Homopentamer. Interacts with RIC3; which is required for proper folding and assembly.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ionic channel (TC 1.A.9) family. [View classification]
Mass spectrometry	Molecular weight is 54157.68 Da from positions 23 - 502. Determined by MALDI. Ref.10

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane
Molecular function	Ionic channel
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	activation of MAPK activity Inferred from direct assay. Source: UniProtKB calcium ion transport Ref.1 Inferred from direct assay. Source: UniProtKB cellular calcium ion homeostasis Inferred from mutant phenotype. Source: UniProtKB memory Non-traceable author statement. Source: UniProtKB negative regulation of inflammatory response Inferred from direct assay. Source: UniProtKB negative regulation of tumor necrosis factor production Inferred from mutant phenotype. Source: UniProtKB positive regulation of angiogenesis Inferred from mutant phenotype. Source: UniProtKB positive regulation of cell proliferation Inferred from mutant phenotype. Source: UniProtKB response to hypoxia Inferred from direct assay. Source: UniProtKB
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW nicotinic acetylcholine-gated receptor-channel complex Ref.3 Inferred from direct assay. Source: UniProtKB postsynaptic membrane Inferred from electronic annotation. Source: InterPro
Molecular function	acetylcholine receptor activity Ref.3 Inferred from direct assay. Source: UniProtKB beta-amyloid binding Inferred from physical interaction. Source: UniProtKB chloride channel regulator activity Ref.1 Inferred from direct assay. Source: UniProtKB nicotinic acetylcholine-activated cation-selective channel activity Ref.1 Ref.3 Inferred from direct assay. Source: UniProtKB protein homodimerization activity Ref.1 Inferred from direct assay. Source: UniProtKB toxin binding Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APP	P05067	1	EBI-79333,EBI-77613

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 502	480	Neuronal acetylcholine receptor subunit alpha-7		PRO_0000000366
Regions
	Topological domain	23 – 230	208	Extracellular Potential
	Transmembrane	231 – 255	25	Potential
	Transmembrane	262 – 280	19	Potential
	Transmembrane	296 – 317	22	Potential
	Topological domain	318 – 469	152	Cytoplasmic Potential
	Transmembrane	470 – 490	21	Potential
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Disulfide bond	150 ↔ 164		By similarity
	Disulfide bond	212 ↔ 213		Associated with receptor activation By similarity
Experimental info
	Sequence conflict	11	1	A → G in CAA49778. Ref.1
	Sequence conflict	58	1	S → N Ref.2 Ref.6
	Sequence conflict	134	1	S → P Ref.2 Ref.6
	Sequence conflict	364	1	C → S in CAA80672. Ref.8
	Sequence conflict	375	1	A → G in CAA49778. Ref.1
	Sequence conflict	409 – 413	5	RMACS → AWPAP in CAA80672. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P36544-1 [UniParc]. Last modified November 1, 1997. Version 5. Checksum: D94B3A482EAA0E42	FASTA	502	56,449
	10 20 30 40 50 60 MRCSPGGVWL ALAASLLHVS LQGEFQRKLY KELVKNYNPL ERPVANDSQP LTVYFSLSLL 70 80 90 100 110 120 QIMDVDEKNQ VLTTNIWLQM SWTDHYLQWN VSEYPGVKTV RFPDGQIWKP DILLYNSADE 130 140 150 160 170 180 RFDATFHTNV LVNSSGHCQY LPPGIFKSSC YIDVRWFPFD VQHCKLKFGS WSYGGWSLDL 190 200 210 220 230 240 QMQEADISGY IPNGEWDLVG IPGKRSERFY ECCKEPYPDV TFTVTMRRRT LYYGLNLLIP 250 260 270 280 290 300 CVLISALALL VFLLPADSGE KISLGITVLL SLTVFMLLVA EIMPATSDSV PLIAQYFAST 310 320 330 340 350 360 MIIVGLSVVV TVIVLQYHHH DPDGGKMPKW TRVILLNWCA WFLRMKRPGE DKVRPACQHK 370 380 390 400 410 420 QRRCSLASVE MSAVAPPPAS NGNLLYIGFR GLDGVHCVPT PDSGVVCGRM ACSPTHDEHL 430 440 450 460 470 480 LHGGQPPEGD PDLAKILEEV RYIANRFRCQ DESEAVCSEW KFAACVVDRL CLMAFSVFTI 490 500 ICTIGILMSA PNFVEAVSKD FA

References

[1]	"Human alpha 7 acetylcholine receptor: cloning of the alpha 7 subunit from the SH-SY5Y cell line and determination of pharmacological properties of native receptors and functional alpha 7 homomers expressed in Xenopus oocytes." Peng X., Katz M., Gerzanich V., Anand R., Lindstrom J. Mol. Pharmacol. 45:546-554(1994) [PubMed: 8145738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	Logel J., Drebing C., Barnhart M., Antle C., Leonard S. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Hippocampus.
[3]	"Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits." Elliott K.J., Ellis S.B., Berckhan K.J., Urrutia A., Chavez-Noriega L.E., Johnson E.C., Velicelebi G., Harpold M.M. J. Mol. Neurosci. 7:217-228(1996) [PubMed: 8906617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32." Groot Kormelink P.J., Luyten W.H.M.L. FEBS Lett. 400:309-314(1997) [PubMed: 9009220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	Groot Kormelink P.J., Luyten W.H.M.L. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[6]	"Cloning cholinergic receptors in human keratinocytes." Arredondo J., Grando S.A. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Keratinocyte.
[7]	"Cloning and sequence of the human a7 nicotinic acetylcholine receptor." Doucette-Stamm L., Monteggia L.M., Donnelly-Roberts D., Wang M.T., Lee J., Tian J., Giordano T. Drug Dev. Res. 30:252-256(1993) Cited for: NUCLEOTIDE SEQUENCE OF 17-502. Tissue: Brain.
[8]	"Molecular cloning and chromosomal localization of the human alpha 7-nicotinic receptor subunit gene (CHRNA7)." Chini B., Raimondi E., Elgoyhen A.B., Moralli D., Balzaretti M., Heinemann S.F. Genomics 19:379-381(1994) [PubMed: 8188270] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-502. Tissue: Retina.
[9]	"A 3-Mb map of a large segmental duplication overlapping the alpha7-nicotinic acetylcholine receptor gene (CHRNA7) at human 15q13-q14." Riley B., Williamson M., Collier D., Wilkie H., Makoff A. Genomics 79:197-209(2002) [PubMed: 11829490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 118-129.
[10]	"Cluster analysis of an extensive human breast cancer cell line protein expression map database." Harris R.A., Yang A., Stein R.C., Lucy K., Brusten L., Herath A., Parekh R., Waterfield M.D., O'Hare M.J., Neville M.A., Page M.J., Zvelebil M.J. Proteomics 2:212-223(2002) [PubMed: 11840567] [Abstract] Cited for: MASS SPECTROMETRY. Tissue: Mammary cancer.
[11]	"Ric-3 promotes functional expression of the nicotinic acetylcholine receptor alpha7 subunit in mammalian cells." Williams M.E., Burton B., Urrutia A., Shcherbatko A., Chavez-Noriega L.E., Cohen C.J., Aiyar J. J. Biol. Chem. 280:1257-1263(2005) [PubMed: 15504725] [Abstract] Cited for: INTERACTION WITH RIC3.
[12]	"RIC-3 enhances functional expression of multiple nicotinic acetylcholine receptor subtypes in mammalian cells." Lansdell S.J., Gee V.J., Harkness P.C., Doward A.I., Baker E.R., Gibb A.J., Millar N.S. Mol. Pharmacol. 68:1431-1438(2005) [PubMed: 16120769] [Abstract] Cited for: INTERACTION WITH RIC3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70297 mRNA. Translation: CAA49778.1. U40583 mRNA. Translation: AAA83561.1. U62436 mRNA. Translation: AAB40114.1. Y08420 mRNA. Translation: CAA69697.1. AF385585 mRNA. Translation: AAK68111.1. L25827 mRNA. No translation available. Z23141 mRNA. Translation: CAA80672.1. AF332758 Genomic DNA. Translation: AAK19515.1.
PIR	G02259. ACHUA7. I37185.
RefSeq	NP_000737.1.
UniGene	Hs.511772
3D structure databases
HSSP	HSSP built from PDB template 1KL8 based on UniProtKB P22770.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36544.
PTM databases
PhosphoSite	P36544.
Genome annotation databases
Ensembl	ENSG00000175344. Homo sapiens. [Contig view]
GeneID	1139.
KEGG	hsa:1139.
Organism-specific databases
H-InvDB	HIX0026783.
HGNC	HGNC:1960. CHRNA7.
MIM	118511. gene+phenotype.
PharmGKB	PA114.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P36544.
HOVERGEN	P36544.
Gene expression databases
CleanEx	HS_CHRNA7.
GermOnline	ENSG00000175344. Homo sapiens.
Family and domain databases
InterPro	IPR006029. Neu_channel_TM. IPR006202. Neur_chan_lig_bd. IPR006201. Neur_channel. IPR002394. Nicotinic_acetylcholine_rcpt_N. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00184. Nicotine. DB01273. Varenicline.
NextBio	4738.
SOURCE	Search...

Entry information

Entry name

ACHA7_HUMAN

Accession

Primary (citable) accession number: P36544
Secondary accession number(s): Q15826 , Q96RH2, Q99555, Q9BXH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 91 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)