P36544 (ACHA7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuronal acetylcholine receptor subunit alpha-7

Gene names

Name:	CHRNA7
Synonyms:	NACHRA7

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Subunit structure	Homopentamer. Interacts with RIC3; which is required for proper folding and assembly. Ref.12 Ref.13
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily. [View classification]
Mass spectrometry	Molecular mass is 54157.68 Da from positions 23 - 502. Determined by MALDI. Ref.11

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ionic channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of MAPK activity Inferred from direct assay. Source: UniProtKB calcium ion transport Inferred from direct assay Ref.1. Source: UniProtKB cellular calcium ion homeostasis Inferred from mutant phenotype. Source: UniProtKB memory Non-traceable author statement. Source: UniProtKB negative regulation of tumor necrosis factor production Inferred from mutant phenotype. Source: UniProtKB positive regulation of angiogenesis Inferred from mutant phenotype. Source: UniProtKB positive regulation of cell proliferation Inferred from mutant phenotype. Source: UniProtKB response to hypoxia Inferred from direct assay. Source: UniProtKB response to nicotine Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	acetylcholine-gated channel complex Inferred from direct assay Ref.3. Source: UniProtKB cell junction Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetylcholine binding Inferred from direct assay Ref.1. Source: UniProtKB acetylcholine receptor activity Inferred from direct assay Ref.3. Source: UniProtKB acetylcholine-activated cation-selective channel activity Inferred from direct assay Ref.1Ref.3. Source: UniProtKB beta-amyloid binding Inferred from physical interaction. Source: UniProtKB chloride channel regulator activity Inferred from direct assay Ref.1. Source: UniProtKB protein homodimerization activity Inferred from direct assay Ref.1. Source: UniProtKB toxin binding Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APP	P05067	1	EBI-79333,EBI-77613

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 502	480	Neuronal acetylcholine receptor subunit alpha-7		PRO_0000000366
Regions
	Topological domain	23 – 230	208	Extracellular Potential
	Transmembrane	231 – 255	25	Helical; Potential
	Transmembrane	262 – 280	19	Helical; Potential
	Transmembrane	296 – 317	22	Helical; Potential
	Topological domain	318 – 469	152	Cytoplasmic Potential
	Transmembrane	470 – 490	21	Helical; Potential
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Disulfide bond	150 ↔ 164		By similarity
	Disulfide bond	212 ↔ 213		Associated with receptor activation By similarity
Experimental info
	Sequence conflict	11	1	A → G in CAA49778. Ref.1
	Sequence conflict	58	1	S → N in AAA83561. Ref.2
	Sequence conflict	58	1	S → N in AAK68111. Ref.6
	Sequence conflict	134	1	S → P in AAA83561. Ref.2
	Sequence conflict	134	1	S → P in AAK68111. Ref.6
	Sequence conflict	364	1	C → S in CAA80672. Ref.9
	Sequence conflict	375	1	A → G in CAA49778. Ref.1
	Sequence conflict	409 – 413	5	RMACS → AWPAP in CAA80672. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P36544 [UniParc]. Last modified November 1, 1997. Version 5. Checksum: D94B3A482EAA0E42	FASTA	502	56,449
	10 20 30 40 50 60 MRCSPGGVWL ALAASLLHVS LQGEFQRKLY KELVKNYNPL ERPVANDSQP LTVYFSLSLL 70 80 90 100 110 120 QIMDVDEKNQ VLTTNIWLQM SWTDHYLQWN VSEYPGVKTV RFPDGQIWKP DILLYNSADE 130 140 150 160 170 180 RFDATFHTNV LVNSSGHCQY LPPGIFKSSC YIDVRWFPFD VQHCKLKFGS WSYGGWSLDL 190 200 210 220 230 240 QMQEADISGY IPNGEWDLVG IPGKRSERFY ECCKEPYPDV TFTVTMRRRT LYYGLNLLIP 250 260 270 280 290 300 CVLISALALL VFLLPADSGE KISLGITVLL SLTVFMLLVA EIMPATSDSV PLIAQYFAST 310 320 330 340 350 360 MIIVGLSVVV TVIVLQYHHH DPDGGKMPKW TRVILLNWCA WFLRMKRPGE DKVRPACQHK 370 380 390 400 410 420 QRRCSLASVE MSAVAPPPAS NGNLLYIGFR GLDGVHCVPT PDSGVVCGRM ACSPTHDEHL 430 440 450 460 470 480 LHGGQPPEGD PDLAKILEEV RYIANRFRCQ DESEAVCSEW KFAACVVDRL CLMAFSVFTI 490 500 ICTIGILMSA PNFVEAVSKD FA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human alpha 7 acetylcholine receptor: cloning of the alpha 7 subunit from the SH-SY5Y cell line and determination of pharmacological properties of native receptors and functional alpha 7 homomers expressed in Xenopus oocytes." Peng X., Katz M., Gerzanich V., Anand R., Lindstrom J. Mol. Pharmacol. 45:546-554(1994) [PubMed: 8145738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Nucleotide sequence and transcript size of the alpha-7 neuronal nicotinic acetylcholine receptor in human postmortem brain." Logel J., Drebing C., Barnhart M., Antle C., Leonard S. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hippocampus.
[3]	"Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits." Elliott K.J., Ellis S.B., Berckhan K.J., Urrutia A., Chavez-Noriega L.E., Johnson E.C., Velicelebi G., Harpold M.M. J. Mol. Neurosci. 7:217-228(1996) [PubMed: 8906617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32." Groot Kormelink P.J., Luyten W.H.M.L. FEBS Lett. 400:309-314(1997) [PubMed: 9009220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	Groot Kormelink P.J., Luyten W.H.M.L. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[6]	"Cloning cholinergic receptors in human keratinocytes." Arredondo J., Grando S.A. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Keratinocyte.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Stomach.
[8]	"Cloning and sequence of the human alpha-7 nicotinic acetylcholine receptor." Doucette-Stamm L., Monteggia L.M., Donnelly-Roberts D., Wang M.T., Lee J., Tian J., Giordano T. Drug Dev. Res. 30:252-256(1993) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-502. Tissue: Brain.
[9]	"Molecular cloning and chromosomal localization of the human alpha 7-nicotinic receptor subunit gene (CHRNA7)." Chini B., Raimondi E., Elgoyhen A.B., Moralli D., Balzaretti M., Heinemann S.F. Genomics 19:379-381(1994) [PubMed: 8188270] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-502. Tissue: Retina.
[10]	"A 3-Mb map of a large segmental duplication overlapping the alpha7-nicotinic acetylcholine receptor gene (CHRNA7) at human 15q13-q14." Riley B., Williamson M., Collier D., Wilkie H., Makoff A. Genomics 79:197-209(2002) [PubMed: 11829490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 118-129.
[11]	"Cluster analysis of an extensive human breast cancer cell line protein expression map database." Harris R.A., Yang A., Stein R.C., Lucy K., Brusten L., Herath A., Parekh R., Waterfield M.D., O'Hare M.J., Neville M.A., Page M.J., Zvelebil M.J. Proteomics 2:212-223(2002) [PubMed: 11840567] [Abstract] Cited for: MASS SPECTROMETRY. Tissue: Mammary cancer.
[12]	"Ric-3 promotes functional expression of the nicotinic acetylcholine receptor alpha7 subunit in mammalian cells." Williams M.E., Burton B., Urrutia A., Shcherbatko A., Chavez-Noriega L.E., Cohen C.J., Aiyar J. J. Biol. Chem. 280:1257-1263(2005) [PubMed: 15504725] [Abstract] Cited for: INTERACTION WITH RIC3.
[13]	"RIC-3 enhances functional expression of multiple nicotinic acetylcholine receptor subtypes in mammalian cells." Lansdell S.J., Gee V.J., Harkness P.C., Doward A.I., Baker E.R., Gibb A.J., Millar N.S. Mol. Pharmacol. 68:1431-1438(2005) [PubMed: 16120769] [Abstract] Cited for: INTERACTION WITH RIC3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70297 mRNA. Translation: CAA49778.1. U40583 mRNA. Translation: AAA83561.1. U62436 mRNA. Translation: AAB40114.1. Y08420 mRNA. Translation: CAA69697.1. AF385585 mRNA. Translation: AAK68111.1. AK292069 mRNA. Translation: BAF84758.1. L25827 mRNA. No translation available. Z23141 mRNA. Translation: CAA80672.1. AF332758 Genomic DNA. Translation: AAK19515.1.
IPI	IPI00942337.
PIR	G02259. ACHUA7. I37185.
RefSeq	NP_000737.1. NM_000746.4.
UniGene	Hs.511772.
3D structure databases
ProteinModelPortal	P36544.
SMR	P36544. Positions 23-490.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36544. 2 interactions.
STRING	P36544.
Protein family/group databases
TCDB	1.A.9.1.1. neurotransmitter receptor, cys loop, ligand-gated ion channel (LIC) family.
PTM databases
PhosphoSite	P36544.
Polymorphism databases
DMDM	2506127.
Proteomic databases
PRIDE	P36544.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306901; ENSP00000303727; ENSG00000175344.
GeneID	1139.
KEGG	hsa:1139.
UCSC	uc001zft.1. human.
Organism-specific databases
CTD	1139.
GeneCards	GC15P030877.
H-InvDB	HIX0026783.
HGNC	HGNC:1960. CHRNA7.
HPA	CAB033624. HPA029422.
MIM	118511. gene+phenotype.
neXtProt	NX_P36544.
Orphanet	199318. 15q13.3 microdeletion syndrome.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09312.
HOVERGEN	HBG003756.
InParanoid	P36544.
OrthoDB	EOG483D4K.
PhylomeDB	P36544.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
Bgee	P36544.
CleanEx	HS_CHRNA7.
Genevestigator	P36544.
GermOnline	ENSG00000175344. Homo sapiens.
Family and domain databases
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
KO	K04809.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00184. Nicotine. DB01273. Varenicline.
NextBio	4738.
SOURCE	Search...

Entry information

Entry name

ACHA7_HUMAN

Accession

Primary (citable) accession number: P36544
Secondary accession number(s): A8K7Q4 , Q15826, Q96RH2, Q99555, Q9BXH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 123 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.