P36543 (VATE1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type proton ATPase subunit E 1
Short name=V-ATPase subunit E 1

Alternative name(s):
V-ATPase 31 kDa subunit
Short name=p31
Vacuolar proton pump subunit E 1

Gene names

Name:	ATP6V1E1
Synonyms:	ATP6E, ATP6E2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Subunit structure	V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with ALDOC. Ref.9
Tissue specificity	Ubiquitous. Ref.10
Sequence similarities	Belongs to the V-ATPase E subunit family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: Compara cellular iron ion homeostasis Traceable author statement. Source: Reactome insulin receptor signaling pathway Traceable author statement. Source: Reactome interaction with host Traceable author statement. Source: Reactome phagosome maturation Traceable author statement. Source: Reactome proton transport Traceable author statement Ref.3. Source: ProtInc transferrin transport Traceable author statement. Source: Reactome transmembrane transport Traceable author statement. Source: Reactome
Cellular_component	apical plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from sequence or structural similarity. Source: UniProtKB endosome Inferred from sequence or structural similarity. Source: UniProtKB microvillus Inferred from electronic annotation. Source: Compara mitochondrion Inferred from electronic annotation. Source: Compara proton-transporting two-sector ATPase complex Traceable author statement Ref.3. Source: ProtInc proton-transporting two-sector ATPase complex, catalytic domain Inferred from electronic annotation. Source: InterPro
Molecular_function	hydrogen-exporting ATPase activity, phosphorylative mechanism Inferred from electronic annotation. Source: Compara proton-transporting ATPase activity, rotational mechanism Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P36543-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P36543-2)

The sequence of this isoform differs from the canonical sequence as follows:
     12-33: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: P36543-3)

The sequence of this isoform differs from the canonical sequence as follows:
     93-122: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	V-type proton ATPase subunit E 1		PRO_0000117295
Amino acid modifications
	Modified residue	56	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	12 – 33	22	Missing in isoform 2.		VSP_042925
	Alternative sequence	93 – 122	30	Missing in isoform 3.		VSP_044589
	Natural variant	50	1	R → G in a colorectal cancer sample; somatic mutation. Ref.12		VAR_036565
Experimental info
	Sequence conflict	32	1	A → R in CAA50592. Ref.3
	Sequence conflict	86	1	A → G no nucleotide entry Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: DFD0D44E6D9AEA17	FASTA	226	26,145
	10 20 30 40 50 60 MALSDADVQK QIKHMMAFIE QEANEKAEEI DAKAEEEFNI EKGRLVQTQR LKIMEYYEKK 70 80 90 100 110 120 EKQIEQQKKI QMSNLMNQAR LKVLRARDDL ITDLLNEAKQ RLSKVVKDTT RYQVLLDGLV 130 140 150 160 170 180 LQGLYQLLEP RMIVRCRKQD FPLVKAAVQK AIPMYKIATK NDVDVQIDQE SYLPEDIAGG 190 200 210 220 VEIYNGDRKI KVSNTLESRL DLIAQQMMPE VRGALFGANA NRKFLD
	Isoform 2 [UniParc]. Checksum: 25B2BADC0BE9B22B	FASTA	204	23,587
	10 20 30 40 50 60 MALSDADVQK QAEEEFNIEK GRLVQTQRLK IMEYYEKKEK QIEQQKKIQM SNLMNQARLK 70 80 90 100 110 120 VLRARDDLIT DLLNEAKQRL SKVVKDTTRY QVLLDGLVLQ GLYQLLEPRM IVRCRKQDFP 130 140 150 160 170 180 LVKAAVQKAI PMYKIATKND VDVQIDQESY LPEDIAGGVE IYNGDRKIKV SNTLESRLDL 190 200 IAQQMMPEVR GALFGANANR KFLD
	Isoform 3 [UniParc]. Checksum: 32EEBB32390D88A2	FASTA	196	22,706
	10 20 30 40 50 60 MALSDADVQK QIKHMMAFIE QEANEKAEEI DAKAEEEFNI EKGRLVQTQR LKIMEYYEKK 70 80 90 100 110 120 EKQIEQQKKI QMSNLMNQAR LKVLRARDDL ITGLYQLLEP RMIVRCRKQD FPLVKAAVQK 130 140 150 160 170 180 AIPMYKIATK NDVDVQIDQE SYLPEDIAGG VEIYNGDRKI KVSNTLESRL DLIAQQMMPE 190 VRGALFGANA NRKFLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Immunologic evidence that vacuolar H+ ATPases with heterogeneous forms of Mr = 31,000 subunit have different membrane distributions in mammalian kidney." Hemken P., Guo X.-L., Wang Z.-Q., Zhang K., Gluck S. J. Biol. Chem. 267:9948-9957(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The E subunit of vacuolar H(+)-ATPase localizes close to the centromere on human chromosome 22." Baud V., Mears A., Lamour V., Scamps C., McDermid A., Lipinski M. Hum. Mol. Genet. 3:335-339(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase." van Hille B., Vanek M., Richener H., Green J.R., Bilbe G. Biochem. Biophys. Res. Commun. 197:15-21(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Osteoclastoma.
[4]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Brain and Cerebellum.
[6]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[8]	Lubec G., Vishwanath V. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 112-131, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[9]	"Interaction between aldolase and vacuolar H+-ATPase: evidence for direct coupling of glycolysis to the ATP-hydrolyzing proton pump." Lu M., Holliday L.S., Zhang L., Dunn W.A. Jr., Gluck S.L. J. Biol. Chem. 276:30407-30413(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ALDOC.
[10]	"A human gene, ATP6E1, encoding a testis-specific isoform of H(+)-ATPase subunit E." Imai-Senga Y., Sun-Wada G.H., Wada Y., Futai M. Gene 289:7-12(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLY-50.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76228 mRNA. Translation: CAA53814.1. X71491 mRNA. Translation: CAA50592.1. CR456385 mRNA. Translation: CAG30271.1. AK294623 mRNA. Translation: BAG57804.1. AK315941 mRNA. Translation: BAH14312.1. AC004019 Genomic DNA. No translation available. AC006285 Genomic DNA. No translation available. AC007666 Genomic DNA. No translation available. BC004443 mRNA. Translation: AAH04443.1.
IPI	IPI00003856. IPI00719366. IPI00719806.
PIR	S60562.
RefSeq	NP_001034455.1. NM_001039366.1. NP_001034456.1. NM_001039367.1. NP_001687.1. NM_001696.3.
UniGene	Hs.517338.
3D structure databases
ProteinModelPortal	P36543.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36543. 6 interactions.
MINT	MINT-5002575.
STRING	9606.ENSP00000253413.
Protein family/group databases
TCDB	3.A.2.2.4. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
PTM databases
PhosphoSite	P36543.
Polymorphism databases
DMDM	549207.
2D gel databases
UCD-2DPAGE	P36543.
Proteomic databases
PaxDb	P36543.
PRIDE	P36543.
Protocols and materials databases
DNASU	529.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000253413; ENSP00000253413; ENSG00000131100. ENST00000399796; ENSP00000382694; ENSG00000131100. ENST00000399798; ENSP00000382696; ENSG00000131100.
GeneID	529.
KEGG	hsa:529.
UCSC	uc002zmr.1. human.
Organism-specific databases
CTD	529.
GeneCards	GC22M018074.
HGNC	HGNC:857. ATP6V1E1.
HPA	CAB009528. CAB018699. HPA029196.
MIM	108746. gene.
neXtProt	NX_P36543.
PharmGKB	PA25158.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1390.
HOGENOM	HOG000202506.
HOVERGEN	HBG002309.
InParanoid	P36543.
KO	K02150.
OMA	TCGGVEL.
OrthoDB	EOG479F86.
PhylomeDB	P36543.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_116125. Disease. REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	P36543.
Bgee	P36543.
CleanEx	HS_ATP6V1E1.
Genevestigator	P36543.
GermOnline	ENSG00000131100. Homo sapiens.
Family and domain databases
InterPro	IPR002842. ATPase_V1/A1-cplx_esu. [Graphical view]
Pfam	PF01991. vATP-synt_E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	529.
NextBio	2197.
SOURCE	Search...

Entry information

Entry name

VATE1_HUMAN

Accession

Primary (citable) accession number: P36543
Secondary accession number(s): A8MUE4, A8MUN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 133 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.