Reviewed, UniProtKB/Swiss-Prot P36543 (VATE1_HUMAN)

Names and origin

Protein names

Recommended name:
    V-type proton ATPase subunit E 1
      Short name=V-ATPase subunit E 1
Alternative name(s):
    Vacuolar proton pump subunit E 1
    V-ATPase 31 kDa subunit
      Short name=P31

Gene names

Name:	ATP6V1E1
Synonyms:	ATP6E, ATP6E2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Subunit structure	V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with ALDOC.
Tissue specificity	Ubiquitous. Ref.8
Sequence similarities	Belongs to the V-ATPase E subunit family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP synthesis coupled proton transport Inferred from electronic annotation. Source: InterPro
Cellular component	apical plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from sequence or structural similarity. Source: UniProtKB endosome Inferred from sequence or structural similarity. Source: UniProtKB proton-transporting two-sector ATPase complex, catalytic domain Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Ref.7 Inferred from physical interaction. Source: UniProtKB proton-transporting ATPase activity, rotational mechanism Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATP6V1G2	O95670	1	EBI-348639,EBI-348290

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 226	225	V-type proton ATPase subunit E 1		PRO_0000117295
Amino acid modifications
	Modified residue	2	1	N-acetylalanine
	Modified residue	56	1	Phosphotyrosine Ref.9
Natural variations
	Natural variant	50	1	R → G in a colorectal cancer sample; somatic mutation. Ref.12		VAR_036565
Experimental info
	Sequence conflict	32	1	A → R in CAA50592. Ref.3
	Sequence conflict	86	1	A → G Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36543-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: DFD0D44E6D9AEA17	FASTA	226	26,145
	10 20 30 40 50 60 MALSDADVQK QIKHMMAFIE QEANEKAEEI DAKAEEEFNI EKGRLVQTQR LKIMEYYEKK 70 80 90 100 110 120 EKQIEQQKKI QMSNLMNQAR LKVLRARDDL ITDLLNEAKQ RLSKVVKDTT RYQVLLDGLV 130 140 150 160 170 180 LQGLYQLLEP RMIVRCRKQD FPLVKAAVQK AIPMYKIATK NDVDVQIDQE SYLPEDIAGG 190 200 210 220 VEIYNGDRKI KVSNTLESRL DLIAQQMMPE VRGALFGANA NRKFLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Immunologic evidence that vacuolar H+ ATPases with heterogeneous forms of Mr = 31,000 subunit have different membrane distributions in mammalian kidney." Hemken P., Guo X.-L., Wang Z.-Q., Zhang K., Gluck S. J. Biol. Chem. 267:9948-9957(1992) [PubMed: 1533641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The E subunit of vacuolar H(+)-ATPase localizes close to the centromere on human chromosome 22." Baud V., Mears A., Lamour V., Scamps C., McDermid A., Lipinski M. Hum. Mol. Genet. 3:335-339(1994) [PubMed: 8004105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase." van Hille B., Vanek M., Richener H., Green J.R., Bilbe G. Biochem. Biophys. Res. Commun. 197:15-21(1993) [PubMed: 8250920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Osteoclastoma.
[4]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	Lubec G., Vishwanath V. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 112-131, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[7]	"Interaction between aldolase and vacuolar H+-ATPase: evidence for direct coupling of glycolysis to the ATP-hydrolyzing proton pump." Lu M., Holliday L.S., Zhang L., Dunn W.A. Jr., Gluck S.L. J. Biol. Chem. 276:30407-30413(2001) [PubMed: 11399750] [Abstract] Cited for: INTERACTION WITH ALDOC.
[8]	"A human gene, ATP6E1, encoding a testis-specific isoform of H(+)-ATPase subunit E." Imai-Senga Y., Sun-Wada G.H., Wada Y., Futai M. Gene 289:7-12(2002) [PubMed: 12036578] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-56, MASS SPECTROMETRY. Tissue: Lung.
[10]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[11]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY.
[12]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLY-50.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76228 mRNA. Translation: CAA53814.1. X71491 mRNA. Translation: CAA50592.1. CR456385 mRNA. Translation: CAG30271.1. BC004443 mRNA. Translation: AAH04443.1.
IPI	IPI00003856.
PIR	S60562.
RefSeq	NP_001687.1.
UniGene	Hs.517338
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P36543. 3 interactions.
STRING	P36543.
Protein family/group databases
TCDB	3.A.2.2.4. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
PTM databases
PhosphoSite	P36543.
Proteomic databases
PRIDE	P36543.
Genome annotation databases
Ensembl	ENST00000253413; ENSP00000253413; ENSG00000131100; Homo sapiens. [Genome view]
GeneID	529.
UCSC	uc002zmr.1. human.
Organism-specific databases
CTD	529.
GeneCards	GC22M016449.
H-InvDB	HIX0016214.
HGNC	HGNC:857. ATP6V1E1.
HPA	CAB009528. CAB018699.
MIM	108746. gene.
PharmGKB	PA25158.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG525538.
HOVERGEN	P36543.
InParanoid	P36543.
OMA	QQRQKIM.
OrthoDB	EOG9H48PB.
Enzyme and pathway databases
BRENDA	3.6.3.14. 247.
Gene expression databases
ArrayExpress	P36543.
Bgee	P36543.
CleanEx	HS_ATP6V1E1.
Genevestigator	P36543.
GermOnline	ENSG00000131100. Homo sapiens.
Family and domain databases
InterPro	IPR002842. ATPase_V1/A1-cplx_esu. [Graphical view]
Pfam	PF01991. vATP-synt_E. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	2197.
SOURCE	Search...

Entry information

Entry name

VATE1_HUMAN

Accession

Primary (citable) accession number: P36543

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 15, 2009
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.