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Protein

54S ribosomal protein L40, mitochondrial

Gene

MRPL40

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-34068-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L40, mitochondrial
Alternative name(s):
YmL40
Gene namesi
Name:MRPL40
Ordered Locus Names:YPL173W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL173W.
SGDiS000006094. MRPL40.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 29729654S ribosomal protein L40, mitochondrialPRO_0000030582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP36534.
PRIDEiP36534.
TopDownProteomicsiP36534.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MRP20P323874EBI-15595,EBI-15599

Protein-protein interaction databases

BioGridi36010. 26 interactions.
DIPiDIP-7947N.
IntActiP36534. 9 interactions.
MINTiMINT-2787372.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20Q1-297[»]
ProteinModelPortaliP36534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 9634KOWAdd
BLAST

Sequence similaritiesi

Contains 1 KOW domain.Curated

Phylogenomic databases

HOGENOMiHOG000000986.
InParanoidiP36534.
KOiK02895.
OMAiRNPYSKH.
OrthoDBiEOG72G1HM.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR005824. KOW.
IPR014722. Rib_L2_dom2.
IPR005825. Ribosomal_L24/26_CS.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
SMARTiSM00739. KOW. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
PROSITEiPS01108. RIBOSOMAL_L24. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSYQHLSN VGSRVMKRLG NRPKNFLPHS EKFIKKSTPE FMKSDLKEVD
60 70 80 90 100
EKTSFKSEKE WKFIPGDRVV VMSGASKGNI AVIKSFDKRT NSFILDENGP
110 120 130 140 150
TKTVPVPKQF WLEGQTSHMI TIPVSILGKD LRLVADIDDE KTPGKTRTVA
160 170 180 190 200
VRDVSFNGSY YDADYKKVMP YRCVKGQPDL IIPWPKPDPI DVQTNLATDP
210 220 230 240 250
VIAREQTFWV DSVVRNPIPK KAIPSIRNPH SKYKRGTLTA KDIAKLVAPE
260 270 280 290
MPLTEVRKSH LAEKKELAER EVPKLTEEDM EAIGARVFEF LEKQKRE
Length:297
Mass (Da):33,751
Last modified:October 1, 1996 - v2
Checksum:iD5BB2B7AE13ECBF9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti179 – 1791D → G in AAT92872 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73529 Genomic DNA. Translation: CAA97880.1.
AY692853 Genomic DNA. Translation: AAT92872.1.
BK006949 Genomic DNA. Translation: DAA11261.1.
PIRiS65185.
RefSeqiNP_015152.1. NM_001183987.1.

Genome annotation databases

EnsemblFungiiYPL173W; YPL173W; YPL173W.
GeneIDi855930.
KEGGisce:YPL173W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73529 Genomic DNA. Translation: CAA97880.1.
AY692853 Genomic DNA. Translation: AAT92872.1.
BK006949 Genomic DNA. Translation: DAA11261.1.
PIRiS65185.
RefSeqiNP_015152.1. NM_001183987.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20Q1-297[»]
ProteinModelPortaliP36534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36010. 26 interactions.
DIPiDIP-7947N.
IntActiP36534. 9 interactions.
MINTiMINT-2787372.

Proteomic databases

MaxQBiP36534.
PRIDEiP36534.
TopDownProteomicsiP36534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL173W; YPL173W; YPL173W.
GeneIDi855930.
KEGGisce:YPL173W.

Organism-specific databases

EuPathDBiFungiDB:YPL173W.
SGDiS000006094. MRPL40.

Phylogenomic databases

HOGENOMiHOG000000986.
InParanoidiP36534.
KOiK02895.
OMAiRNPYSKH.
OrthoDBiEOG72G1HM.

Enzyme and pathway databases

BioCyciYEAST:G3O-34068-MONOMER.

Miscellaneous databases

PROiP36534.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR005824. KOW.
IPR014722. Rib_L2_dom2.
IPR005825. Ribosomal_L24/26_CS.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
SMARTiSM00739. KOW. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
PROSITEiPS01108. RIBOSOMAL_L24. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
    Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
    FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 109-129 AND 146-158, SUBUNIT.
    Strain: 07173.
  5. "The yeast mitochondrial degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism."
    Dziembowski A., Piwowarski J., Hoser R., Minczuk M., Dmochowska A., Siep M., van der Spek H., Grivell L.A., Stepien P.P.
    J. Biol. Chem. 278:1603-1611(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 176-215 AND 287-293.
  6. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
    Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
    Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRM40_YEAST
AccessioniPrimary (citable) accession number: P36534
Secondary accession number(s): D6W3J5, Q6B277
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.