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Protein

54S ribosomal protein L36, mitochondrial

Gene

MRPL36

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in mitochondrial translation. Overexpression suppresses mutations in the COX2 leader peptide-encoding and initiation codon regions.2 Publications

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29079-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L36, mitochondrial
Alternative name(s):
YmL36
Gene namesi
Name:MRPL36
Ordered Locus Names:YBR122C
ORF Names:YBR0918
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome II

Organism-specific databases

CYGDiYBR122c.
SGDiS000000326. MRPL36.

Subcellular locationi

Mitochondrion 2 Publications

GO - Cellular componenti

  1. mitochondrial large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1414Mitochondrion1 PublicationAdd
BLAST
Chaini15 – 17716354S ribosomal protein L36, mitochondrialPRO_0000030580Add
BLAST

Proteomic databases

MaxQBiP36531.
PaxDbiP36531.
PeptideAtlasiP36531.

Expressioni

Gene expression databases

GenevestigatoriP36531.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LSB3P436031EBI-15582,EBI-22980
YSC84P327931EBI-15582,EBI-24460

Protein-protein interaction databases

BioGridi32824. 47 interactions.
DIPiDIP-4628N.
IntActiP36531. 13 interactions.
MINTiMINT-566842.
STRINGi4932.YBR122C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20V1-177[»]
ProteinModelPortaliP36531.
SMRiP36531. Positions 20-83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 11883Sufficient for general mitochondrial translationAdd
BLAST
Regioni87 – 17791Sufficient for dosage suppression of COX2 mutationAdd
BLAST

Domaini

Contains two functional domains. The central domain is sufficient for general mitochondrial translation but not suppression of COX2 mutants. The C-terminus sequence is sufficient for dosage suppression of COX2 mutants.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG42857.
HOGENOMiHOG000248746.
InParanoidiP36531.
OMAiPLWNPSR.
OrthoDBiEOG7WT4D3.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36531-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKSIFAKRF ASTGSYPGST RITLPRRPAK KIQLGKSRPA IYHQFNVKME
60 70 80 90 100
LSDGSVVIRR SQYPKGEIRL IQDQRNNPLW NPSRDDLVVV DANSGGSLDR
110 120 130 140 150
FNKRYSSLFS VDSTTPNSSS ETVELSEENK KKTQIKKEEK EDVSEKAFGM
160 170
DDYLSLLDDS EQQIKSGKLA SKKRDKK
Length:177
Mass (Da):20,091
Last modified:December 20, 2005 - v3
Checksum:i7A8DF86EE899B848
GO

Sequence cautioni

The sequence CAA55624.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA85079.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78993 Genomic DNA. Translation: CAA55624.1. Different initiation.
Z35991 Genomic DNA. Translation: CAA85079.1. Different initiation.
BK006936 Genomic DNA. Translation: DAA07240.1.
PIRiS44701.
RefSeqiNP_009680.2. NM_001178470.1.

Genome annotation databases

EnsemblFungiiYBR122C; YBR122C; YBR122C.
GeneIDi852419.
KEGGisce:YBR122C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78993 Genomic DNA. Translation: CAA55624.1. Different initiation.
Z35991 Genomic DNA. Translation: CAA85079.1. Different initiation.
BK006936 Genomic DNA. Translation: DAA07240.1.
PIRiS44701.
RefSeqiNP_009680.2. NM_001178470.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20V1-177[»]
ProteinModelPortaliP36531.
SMRiP36531. Positions 20-83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32824. 47 interactions.
DIPiDIP-4628N.
IntActiP36531. 13 interactions.
MINTiMINT-566842.
STRINGi4932.YBR122C.

Proteomic databases

MaxQBiP36531.
PaxDbiP36531.
PeptideAtlasiP36531.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR122C; YBR122C; YBR122C.
GeneIDi852419.
KEGGisce:YBR122C.

Organism-specific databases

CYGDiYBR122c.
SGDiS000000326. MRPL36.

Phylogenomic databases

eggNOGiNOG42857.
HOGENOMiHOG000248746.
InParanoidiP36531.
OMAiPLWNPSR.
OrthoDBiEOG7WT4D3.

Enzyme and pathway databases

BioCyciYEAST:G3O-29079-MONOMER.

Miscellaneous databases

NextBioi971282.

Gene expression databases

GenevestigatoriP36531.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 10:1363-1381(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
    Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
    FEBS Lett. 284:51-56(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 15-51, SUBUNIT.
    Strain: 07173.
  5. "Mitochondrial translation of Saccharomyces cerevisiae COX2 mRNA is controlled by the nucleotide sequence specifying the pre-Cox2p leader peptide."
    Bonnefoy N., Bsat N., Fox T.D.
    Mol. Cell. Biol. 21:2359-2372(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
    Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
    Eur. J. Biochem. 269:5203-5214(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  10. "MrpL36p, a highly diverged L31 ribosomal protein homolog with additional functional domains in Saccharomyces cerevisiae mitochondria."
    Williams E.H., Perez-Martinez X., Fox T.D.
    Genetics 167:65-75(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN.

Entry informationi

Entry nameiRM36_YEAST
AccessioniPrimary (citable) accession number: P36531
Secondary accession number(s): D6VQC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 20, 2005
Last modified: March 4, 2015
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.