P36531 (RM36_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L36, mitochondrial

Alternative name(s):
YmL36

Gene names

Name:	MRPL36
Ordered Locus Names:	YBR122C
ORF Names:	YBR0918

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in mitochondrial translation. Overexpression suppresses mutations in the COX2 leader peptide-encoding and initiation codon regions. Ref.5 Ref.10
Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.4 Ref.6
Subcellular location	Mitochondrion Ref.8 Ref.9.
Domain	Contains two functional domains. The central domain is sufficient for general mitochondrial translation but not suppression of COX2 mutants. The C-terminus sequence is sufficient for dosage suppression of COX2 mutants. Ref.10
Sequence similarities	Belongs to the ribosomal protein L31P family. Highly divergent.
Sequence caution	The sequence CAA55624.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA85079.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial translation Inferred by curator Ref.6. Source: SGD
Cellular_component	mitochondrial large ribosomal subunit Inferred from physical interaction Ref.6. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from physical interaction Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LSB3	P43603	1	EBI-15582,EBI-22980
YSC84	P32793	1	EBI-15582,EBI-24460

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 14	14	Mitochondrion Ref.4
	Chain	15 – 177	163	54S ribosomal protein L36, mitochondrial		PRO_0000030580
Regions
	Region	36 – 118	83	Sufficient for general mitochondrial translation
	Region	87 – 177	91	Sufficient for dosage suppression of COX2 mutation

Sequences

	Sequence		Length	Mass (Da)
	P36531 [UniParc]. Last modified December 20, 2005. Version 3. Checksum: 7A8DF86EE899B848	FASTA	177	20,091
	10 20 30 40 50 60 MLKSIFAKRF ASTGSYPGST RITLPRRPAK KIQLGKSRPA IYHQFNVKME LSDGSVVIRR 70 80 90 100 110 120 SQYPKGEIRL IQDQRNNPLW NPSRDDLVVV DANSGGSLDR FNKRYSSLFS VDSTTPNSSS 130 140 150 160 170 ETVELSEENK KKTQIKKEEK EDVSEKAFGM DDYLSLLDDS EQQIKSGKLA SKKRDKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria." Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M. FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 15-51, SUBUNIT. Strain: 07173.
[5]	"Mitochondrial translation of Saccharomyces cerevisiae COX2 mRNA is controlled by the nucleotide sequence specifying the pre-Cox2p leader peptide." Bonnefoy N., Bsat N., Fox T.D. Mol. Cell. Biol. 21:2359-2372(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, MASS SPECTROMETRY.
[7]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[10]	"MrpL36p, a highly diverged L31 ribosomal protein homolog with additional functional domains in Saccharomyces cerevisiae mitochondria." Williams E.H., Perez-Martinez X., Fox T.D. Genetics 167:65-75(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DOMAIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78993 Genomic DNA. Translation: CAA55624.1. Different initiation. Z35991 Genomic DNA. Translation: CAA85079.1. Different initiation. BK006936 Genomic DNA. Translation: DAA07240.1.
PIR	S44701.
RefSeq	NP_009680.2. NM_001178470.1.
3D structure databases
ProteinModelPortal	P36531.
SMR	P36531. Positions 46-108.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4628N.
IntAct	P36531. 8 interactions.
MINT	MINT-566842.
STRING	4932.YBR122C.
Proteomic databases
PaxDb	P36531.
PeptideAtlas	P36531.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR122C; YBR122C; YBR122C.
GeneID	852419.
KEGG	sce:YBR122C.
Organism-specific databases
CYGD	YBR122c.
SGD	S000000326. MRPL36.
Phylogenomic databases
eggNOG	NOG42857.
HOGENOM	HOG000248746.
OMA	NPLWNPS.
OrthoDB	EOG45F103.
Gene expression databases
Genevestigator	P36531.
GermOnline	YBR122C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	971282.

Entry information

Entry name

RM36_YEAST

Accession

Primary (citable) accession number: P36531
Secondary accession number(s): D6VQC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	December 20, 2005
Last modified:	April 3, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program