P36528 (RM17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L17, mitochondrial

Alternative name(s):
YmL17/YmL30

Gene names

Name:	MRPL17
Synonyms:	MRPL30
Ordered Locus Names:	YNL252C
ORF Names:	N0864

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.5 Ref.6 Ref.7
Subcellular location	Mitochondrion Ref.8 Ref.10.
Miscellaneous	Present with 7400 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ribosomal protein L46 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial translation Inferred by curator Ref.7Ref.6. Source: SGD
Cellular_component	mitochondrial large ribosomal subunit Inferred from direct assay Ref.6. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 19	19	Mitochondrion Ref.5
	Chain	20 – 281	262	54S ribosomal protein L17, mitochondrial		PRO_0000030578

Sequences

	Sequence		Length	Mass (Da)
	P36528 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: BD4F2B6D7C2F44DB	FASTA	281	32,213
	10 20 30 40 50 60 MKVNLMLKRG LATATATASS APPKIKVGVL LSRIPIIKSE LNELEKKYYE YQSELEKRLM 70 80 90 100 110 120 WTFPAYFYFK KGTVAEHKFL SLQKGPISKK NGIWFPRGIP DIKHGRERST KQEVKLSDDS 130 140 150 160 170 180 TVAFSNNQKE QSKDDVNRPV IPNDRITEAD RSNDMKSLER QLSRTLYLLV KDKSGTWKFP 190 200 210 220 230 240 NFDLSDESKP LHVHAENELK LLSGDQIYTW SVSATPIGVL QDERNRTAEF IVKSHILAGK 250 260 270 280 FDLVASKNDA FEDFAWLTKG EISEYVPKDY FNKTEFLLAD N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae." Sen-Gupta M., Gueldener U., Beinhauer J.D., Fiedler T.A., Hegemann J.H. Yeast 13:849-860(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria." Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M. FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-40, SUBUNIT. Strain: 07173.
[6]	"Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae." Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K. Eur. J. Biochem. 245:449-456(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 157-169, SUBUNIT. Strain: 07173.
[7]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, MASS SPECTROMETRY.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96722 Genomic DNA. Translation: CAA65492.1. Z71528 Genomic DNA. Translation: CAA96159.1. AY558015 Genomic DNA. Translation: AAS56341.1. BK006947 Genomic DNA. Translation: DAA10307.1.
PIR	S63225.
RefSeq	NP_014147.1. NM_001183090.1.
3D structure databases
ProteinModelPortal	P36528.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4278N.
IntAct	P36528. 7 interactions.
MINT	MINT-487311.
STRING	4932.YNL252C.
Proteomic databases
PaxDb	P36528.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL252C; YNL252C; YNL252C.
GeneID	855469.
KEGG	sce:YNL252C.
Organism-specific databases
CYGD	YNL252c.
SGD	S000005196. MRPL17.
Phylogenomic databases
eggNOG	NOG256490.
HOGENOM	HOG000248758.
OMA	RLMWTFP.
OrthoDB	EOG47H909.
Gene expression databases
Genevestigator	P36528.
GermOnline	YNL252C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021757. Ribosomal_L46. [Graphical view]
Pfam	PF11788. MRP-L46. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979412.

Entry information

Entry name

RM17_YEAST

Accession

Primary (citable) accession number: P36528
Secondary accession number(s): D6W0U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program