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P36526

- RM27_YEAST

UniProt

P36526 - RM27_YEAST

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Protein
54S ribosomal protein L27, mitochondrial
Gene
MRPL27, YBR282W, YBR2019
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29202-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L27, mitochondrial
Alternative name(s):
YmL27
Gene namesi
Name:MRPL27
Ordered Locus Names:YBR282W
ORF Names:YBR2019
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR282w.
SGDiS000000486. MRPL27.

Subcellular locationi

Mitochondrion 2 Publications

GO - Cellular componenti

  1. mitochondrial large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1616Mitochondrion2 Publications
Add
BLAST
Chaini17 – 14613054S ribosomal protein L27, mitochondrial
PRO_0000030574Add
BLAST

Proteomic databases

MaxQBiP36526.
PaxDbiP36526.
PeptideAtlasiP36526.

Expressioni

Gene expression databases

GenevestigatoriP36526.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

Protein-protein interaction databases

BioGridi32976. 22 interactions.
DIPiDIP-4961N.
IntActiP36526. 7 interactions.
MINTiMINT-511392.
STRINGi4932.YBR282W.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW4electron microscopy3.2031-146[»]
ProteinModelPortaliP36526.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG282990.
HOGENOMiHOG000247918.
KOiK17422.
OMAiANLRRPW.
OrthoDBiEOG769ZX8.

Family and domain databases

InterProiIPR019189. Ribosomal_L27/L41_mit.
[Graphical view]
PfamiPF09809. MRP-L27. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36526-1 [UniParc]FASTAAdd to Basket

« Hide

MKGSPISQFS KTSINALTRP WKKYRDGELF YGLSKVGNKR VPLTTKQGNK    50
TMYKGTRASG IGRHTKFGGY VINWKKVRTY VTPDMVNFEL KPYVNANVPP 100
LKHEFKGFSG GPLDPRLQLL KIKEYIVNGR VQSEGATDTS CYKERG 146
Length:146
Mass (Da):16,492
Last modified:October 1, 1994 - v2
Checksum:i0BB2E6D8A0E49213
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171L → T in AAB21096. 1 Publication
Sequence conflicti21 – 211W → C AA sequence 1 Publication
Sequence conflicti48 – 481G → H AA sequence 1 Publication
Sequence conflicti57 – 571R → N AA sequence 1 Publication
Sequence conflicti72 – 721I → M in AAB21096. 1 Publication
Sequence conflicti77 – 771V → Y AA sequence 1 Publication
Sequence conflicti101 – 1011L → T in AAB21096. 1 Publication
Sequence conflicti122 – 1221I → M in AAB21096. 1 Publication
Sequence conflicti126 – 1261I → M in AAB21096. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S77888 Genomic DNA. Translation: AAB21096.2.
X76053 Genomic DNA. Translation: CAA53645.1.
Z36151 Genomic DNA. Translation: CAA85246.1.
BK006936 Genomic DNA. Translation: DAA07397.1.
PIRiS27285.
RefSeqiNP_009841.1. NM_001178630.1.

Genome annotation databases

EnsemblFungiiYBR282W; YBR282W; YBR282W.
GeneIDi852585.
KEGGisce:YBR282W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S77888 Genomic DNA. Translation: AAB21096.2 .
X76053 Genomic DNA. Translation: CAA53645.1 .
Z36151 Genomic DNA. Translation: CAA85246.1 .
BK006936 Genomic DNA. Translation: DAA07397.1 .
PIRi S27285.
RefSeqi NP_009841.1. NM_001178630.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VW4 electron microscopy 3.20 3 1-146 [» ]
ProteinModelPortali P36526.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32976. 22 interactions.
DIPi DIP-4961N.
IntActi P36526. 7 interactions.
MINTi MINT-511392.
STRINGi 4932.YBR282W.

Proteomic databases

MaxQBi P36526.
PaxDbi P36526.
PeptideAtlasi P36526.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR282W ; YBR282W ; YBR282W .
GeneIDi 852585.
KEGGi sce:YBR282W.

Organism-specific databases

CYGDi YBR282w.
SGDi S000000486. MRPL27.

Phylogenomic databases

eggNOGi NOG282990.
HOGENOMi HOG000247918.
KOi K17422.
OMAi ANLRRPW.
OrthoDBi EOG769ZX8.

Enzyme and pathway databases

BioCyci YEAST:G3O-29202-MONOMER.

Miscellaneous databases

NextBioi 971736.
PROi P36526.

Gene expression databases

Genevestigatori P36526.

Family and domain databases

InterProi IPR019189. Ribosomal_L27/L41_mit.
[Graphical view ]
Pfami PF09809. MRP-L27. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast."
    Graack H.-R., Grohmann L., Kitakawa M.
    Biochimie 73:837-844(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-32; 35-40; 46-55 AND 77-92.
  2. "The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Holmstroem K., Brandt T., Kallesoe T.
    Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
    Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
    FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-57 AND 77-92, SUBUNIT.
    Strain: 07173.
  6. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
    Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
    Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiRM27_YEAST
AccessioniPrimary (citable) accession number: P36526
Secondary accession number(s): D6VQS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2250 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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