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P36526 (RM27_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
54S ribosomal protein L27, mitochondrial
Alternative name(s):
YmL27
Gene names
Name:MRPL27
Ordered Locus Names:YBR282W
ORF Names:YBR2019
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length146 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.5 Ref.6

Subcellular location

Mitochondrion Ref.7 Ref.9.

Miscellaneous

Present with 2250 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ribosomal protein L41 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1616Mitochondrion Ref.1 Ref.5
Chain17 – 14613054S ribosomal protein L27, mitochondrial
PRO_0000030574

Experimental info

Sequence conflict171L → T in AAB21096. Ref.1
Sequence conflict211W → C AA sequence Ref.1
Sequence conflict481G → H AA sequence Ref.1
Sequence conflict571R → N AA sequence Ref.5
Sequence conflict721I → M in AAB21096. Ref.1
Sequence conflict771V → Y AA sequence Ref.5
Sequence conflict1011L → T in AAB21096. Ref.1
Sequence conflict1221I → M in AAB21096. Ref.1
Sequence conflict1261I → M in AAB21096. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P36526 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: 0BB2E6D8A0E49213

FASTA14616,492
        10         20         30         40         50         60 
MKGSPISQFS KTSINALTRP WKKYRDGELF YGLSKVGNKR VPLTTKQGNK TMYKGTRASG 

        70         80         90        100        110        120 
IGRHTKFGGY VINWKKVRTY VTPDMVNFEL KPYVNANVPP LKHEFKGFSG GPLDPRLQLL 

       130        140 
KIKEYIVNGR VQSEGATDTS CYKERG 

« Hide

References

« Hide 'large scale' references
[1]"The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast."
Graack H.-R., Grohmann L., Kitakawa M.
Biochimie 73:837-844(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-32; 35-40; 46-55 AND 77-92.
[2]"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
Holmstroem K., Brandt T., Kallesoe T.
Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 17-57 AND 77-92, SUBUNIT.
Strain: 07173.
[6]"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S77888 Genomic DNA. Translation: AAB21096.2.
X76053 Genomic DNA. Translation: CAA53645.1.
Z36151 Genomic DNA. Translation: CAA85246.1.
BK006936 Genomic DNA. Translation: DAA07397.1.
PIRS27285.
RefSeqNP_009841.1. NM_001178630.1.

3D structure databases

ProteinModelPortalP36526.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32976. 22 interactions.
DIPDIP-4961N.
IntActP36526. 7 interactions.
MINTMINT-511392.
STRING4932.YBR282W.

Proteomic databases

PaxDbP36526.
PeptideAtlasP36526.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR282W; YBR282W; YBR282W.
GeneID852585.
KEGGsce:YBR282W.

Organism-specific databases

CYGDYBR282w.
SGDS000000486. MRPL27.

Phylogenomic databases

eggNOGNOG282990.
HOGENOMHOG000247918.
KOK17422.
OMALRRPWQT.
OrthoDBEOG769ZX8.

Enzyme and pathway databases

BioCycYEAST:G3O-29202-MONOMER.

Gene expression databases

GenevestigatorP36526.

Family and domain databases

InterProIPR019189. Ribosomal_L27/L41_mit.
[Graphical view]
PfamPF09809. MRP-L27. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971736.
PROP36526.

Entry information

Entry nameRM27_YEAST
AccessionPrimary (citable) accession number: P36526
Secondary accession number(s): D6VQS7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries