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P36526

- RM27_YEAST

UniProt

P36526 - RM27_YEAST

Protein

54S ribosomal protein L27, mitochondrial

Gene

MRPL27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. mitochondrial translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29202-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    54S ribosomal protein L27, mitochondrial
    Alternative name(s):
    YmL27
    Gene namesi
    Name:MRPL27
    Ordered Locus Names:YBR282W
    ORF Names:YBR2019
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR282w.
    SGDiS000000486. MRPL27.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. mitochondrial large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1616Mitochondrion2 PublicationsAdd
    BLAST
    Chaini17 – 14613054S ribosomal protein L27, mitochondrialPRO_0000030574Add
    BLAST

    Proteomic databases

    MaxQBiP36526.
    PaxDbiP36526.
    PeptideAtlasiP36526.

    Expressioni

    Gene expression databases

    GenevestigatoriP36526.

    Interactioni

    Subunit structurei

    Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

    Protein-protein interaction databases

    BioGridi32976. 22 interactions.
    DIPiDIP-4961N.
    IntActiP36526. 7 interactions.
    MINTiMINT-511392.
    STRINGi4932.YBR282W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW4electron microscopy3.2031-146[»]
    ProteinModelPortaliP36526.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L41 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiNOG282990.
    HOGENOMiHOG000247918.
    KOiK17422.
    OMAiANLRRPW.
    OrthoDBiEOG769ZX8.

    Family and domain databases

    InterProiIPR019189. Ribosomal_L27/L41_mit.
    [Graphical view]
    PfamiPF09809. MRP-L27. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36526-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGSPISQFS KTSINALTRP WKKYRDGELF YGLSKVGNKR VPLTTKQGNK    50
    TMYKGTRASG IGRHTKFGGY VINWKKVRTY VTPDMVNFEL KPYVNANVPP 100
    LKHEFKGFSG GPLDPRLQLL KIKEYIVNGR VQSEGATDTS CYKERG 146
    Length:146
    Mass (Da):16,492
    Last modified:October 1, 1994 - v2
    Checksum:i0BB2E6D8A0E49213
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171L → T in AAB21096. (PubMed:1764528)Curated
    Sequence conflicti21 – 211W → C AA sequence (PubMed:1764528)Curated
    Sequence conflicti48 – 481G → H AA sequence (PubMed:1764528)Curated
    Sequence conflicti57 – 571R → N AA sequence (PubMed:2060626)Curated
    Sequence conflicti72 – 721I → M in AAB21096. (PubMed:1764528)Curated
    Sequence conflicti77 – 771V → Y AA sequence (PubMed:2060626)Curated
    Sequence conflicti101 – 1011L → T in AAB21096. (PubMed:1764528)Curated
    Sequence conflicti122 – 1221I → M in AAB21096. (PubMed:1764528)Curated
    Sequence conflicti126 – 1261I → M in AAB21096. (PubMed:1764528)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S77888 Genomic DNA. Translation: AAB21096.2.
    X76053 Genomic DNA. Translation: CAA53645.1.
    Z36151 Genomic DNA. Translation: CAA85246.1.
    BK006936 Genomic DNA. Translation: DAA07397.1.
    PIRiS27285.
    RefSeqiNP_009841.1. NM_001178630.1.

    Genome annotation databases

    EnsemblFungiiYBR282W; YBR282W; YBR282W.
    GeneIDi852585.
    KEGGisce:YBR282W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S77888 Genomic DNA. Translation: AAB21096.2 .
    X76053 Genomic DNA. Translation: CAA53645.1 .
    Z36151 Genomic DNA. Translation: CAA85246.1 .
    BK006936 Genomic DNA. Translation: DAA07397.1 .
    PIRi S27285.
    RefSeqi NP_009841.1. NM_001178630.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW4 electron microscopy 3.20 3 1-146 [» ]
    ProteinModelPortali P36526.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32976. 22 interactions.
    DIPi DIP-4961N.
    IntActi P36526. 7 interactions.
    MINTi MINT-511392.
    STRINGi 4932.YBR282W.

    Proteomic databases

    MaxQBi P36526.
    PaxDbi P36526.
    PeptideAtlasi P36526.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR282W ; YBR282W ; YBR282W .
    GeneIDi 852585.
    KEGGi sce:YBR282W.

    Organism-specific databases

    CYGDi YBR282w.
    SGDi S000000486. MRPL27.

    Phylogenomic databases

    eggNOGi NOG282990.
    HOGENOMi HOG000247918.
    KOi K17422.
    OMAi ANLRRPW.
    OrthoDBi EOG769ZX8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29202-MONOMER.

    Miscellaneous databases

    NextBioi 971736.
    PROi P36526.

    Gene expression databases

    Genevestigatori P36526.

    Family and domain databases

    InterProi IPR019189. Ribosomal_L27/L41_mit.
    [Graphical view ]
    Pfami PF09809. MRP-L27. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast."
      Graack H.-R., Grohmann L., Kitakawa M.
      Biochimie 73:837-844(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-32; 35-40; 46-55 AND 77-92.
    2. "The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
      Holmstroem K., Brandt T., Kallesoe T.
      Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
      Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
      FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 17-57 AND 77-92, SUBUNIT.
      Strain: 07173.
    6. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
      Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
      Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiRM27_YEAST
    AccessioniPrimary (citable) accession number: P36526
    Secondary accession number(s): D6VQS7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2250 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3