P36526 (RM27_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L27, mitochondrial

Alternative name(s):
YmL27

Gene names

Name:	MRPL27
Ordered Locus Names:	YBR282W
ORF Names:	YBR2019

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.5 Ref.6
Subcellular location	Mitochondrion Ref.7 Ref.9.
Miscellaneous	Present with 2250 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ribosomal protein L41 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial translation Inferred by curator Ref.6. Source: SGD
Cellular_component	mitochondrial large ribosomal subunit Inferred from physical interaction Ref.6. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from physical interaction Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 16	16	Mitochondrion Ref.1 Ref.5
	Chain	17 – 146	130	54S ribosomal protein L27, mitochondrial		PRO_0000030574
Experimental info
	Sequence conflict	17	1	L → T in AAB21096. Ref.1
	Sequence conflict	21	1	W → C AA sequence Ref.1
	Sequence conflict	48	1	G → H AA sequence Ref.1
	Sequence conflict	57	1	R → N AA sequence Ref.5
	Sequence conflict	72	1	I → M in AAB21096. Ref.1
	Sequence conflict	77	1	V → Y AA sequence Ref.5
	Sequence conflict	101	1	L → T in AAB21096. Ref.1
	Sequence conflict	122	1	I → M in AAB21096. Ref.1
	Sequence conflict	126	1	I → M in AAB21096. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36526 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 0BB2E6D8A0E49213	FASTA	146	16,492
	10 20 30 40 50 60 MKGSPISQFS KTSINALTRP WKKYRDGELF YGLSKVGNKR VPLTTKQGNK TMYKGTRASG 70 80 90 100 110 120 IGRHTKFGGY VINWKKVRTY VTPDMVNFEL KPYVNANVPP LKHEFKGFSG GPLDPRLQLL 130 140 KIKEYIVNGR VQSEGATDTS CYKERG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast." Graack H.-R., Grohmann L., Kitakawa M. Biochimie 73:837-844(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-32; 35-40; 46-55 AND 77-92.
[2]	"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Holmstroem K., Brandt T., Kallesoe T. Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria." Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M. FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-57 AND 77-92, SUBUNIT. Strain: 07173.
[6]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, MASS SPECTROMETRY.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S77888 Genomic DNA. Translation: AAB21096.2. X76053 Genomic DNA. Translation: CAA53645.1. Z36151 Genomic DNA. Translation: CAA85246.1. BK006936 Genomic DNA. Translation: DAA07397.1.
PIR	S27285.
RefSeq	NP_009841.1. NM_001178630.1.
3D structure databases
ProteinModelPortal	P36526.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4961N.
IntAct	P36526. 4 interactions.
MINT	MINT-511392.
STRING	4932.YBR282W.
Proteomic databases
PaxDb	P36526.
PeptideAtlas	P36526.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR282W; YBR282W; YBR282W.
GeneID	852585.
KEGG	sce:YBR282W.
Organism-specific databases
CYGD	YBR282w.
SGD	S000000486. MRPL27.
Phylogenomic databases
eggNOG	NOG282990.
HOGENOM	HOG000247918.
OMA	GNKHYYK.
OrthoDB	EOG4C5GV1.
Gene expression databases
Genevestigator	P36526.
GermOnline	YBR282W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019189. Ribosomal_L27/L41_mit. [Graphical view]
Pfam	PF09809. MRP-L27. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971736.

Entry information

Entry name

RM27_YEAST

Accession

Primary (citable) accession number: P36526
Secondary accession number(s): D6VQS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program