P36520 (RM10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L10, mitochondrial

Alternative name(s):
YmL10/YmL18

Gene names

Name:	MRPL10
Synonyms:	MRPL18
Ordered Locus Names:	YNL284C
ORF Names:	N0580

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.3 Ref.4
Subcellular location	Mitochondrion Ref.5 Ref.7.
Miscellaneous	Present with 5350 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ribosomal protein L15P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial translation Inferred by curator Ref.4. Source: SGD
Cellular_component	mitochondrial large ribosomal subunit Inferred from direct assay Ref.4. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 57	57	Mitochondrion Ref.3
	Chain	58 – 322	265	54S ribosomal protein L10, mitochondrial		PRO_0000030468
Experimental info
	Sequence conflict	68	1	D → K AA sequence Ref.3
	Sequence conflict	71	1	T → G AA sequence Ref.3
	Sequence conflict	182	1	F → I AA sequence Ref.4
	Sequence conflict	188	1	I → K AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36520 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: FD56AB98F62B4A07	FASTA	322	36,347
	10 20 30 40 50 60 MKAERQTGLR NSFTTVIGRK LINTFVPSMM LTSVAGNDIF FRGLFKSPVL AFQSYRYVSI 70 80 90 100 110 120 LGQLKPSDGS TKSFKRLGRG PSSGLGKTSG RGQKGQKARG KVKSWFEGGQ TPIYKLFPKI 130 140 150 160 170 180 GFTNVGAKPL KELNLKRIQW FHDKNRLHLQ PGEVLDMNKM RKLGLVTGPI KYGVKILASG 190 200 210 220 230 240 KFHYNLPIAL EASRASAKAI AAIEKAGGKF TARYYTPLGL RAHLNPQWFL EKRGRVPLQA 250 260 270 280 290 300 RPTKRRDIDF YSKEEKRGYL VMEKDKLLQD IKEAQNKGSR HFLKQNVKKS SLEIELEELS 310 320 PEKDWVPVVS NSKVMNIKAL DH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria." Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M. FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 58-71, SUBUNIT. Strain: 07173.
[4]	"Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae." Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K. Eur. J. Biochem. 245:449-456(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 182-195 AND 247-253, SUBUNIT. Strain: 07173.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71560 Genomic DNA. Translation: CAA96198.1. BK006947 Genomic DNA. Translation: DAA10276.1.
PIR	S63258.
RefSeq	NP_014115.1. NM_001183122.1.
3D structure databases
ProteinModelPortal	P36520.
SMR	P36520. Positions 64-209.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6771N.
IntAct	P36520. 37 interactions.
MINT	MINT-613283.
STRING	4932.YNL284C.
Proteomic databases
PaxDb	P36520.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL284C; YNL284C; YNL284C.
GeneID	855436.
KEGG	sce:YNL284C.
Organism-specific databases
CYGD	YNL284c.
SGD	S000005228. MRPL10.
Phylogenomic databases
eggNOG	COG0200.
GeneTree	ENSGT00390000009040.
HOGENOM	HOG000231264.
KO	K02876.
OMA	NDIFFRG.
OrthoDB	EOG4NW2VM.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33275-MONOMER.
Gene expression databases
Genevestigator	P36520.
GermOnline	YNL284C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005749. Ribosomal_L15_bac-type. IPR021131. Ribosomal_L18e/L15P. [Graphical view]
PANTHER	PTHR12934. PTHR12934. 1 hit.
Pfam	PF00828. Ribosomal_L18e. 1 hit. [Graphical view]
SUPFAM	SSF52080. Ribosomal_L18e/L15. 1 hit.
TIGRFAMs	TIGR01071. rplO_bact. 1 hit.
PROSITE	PS00475. RIBOSOMAL_L15. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	979320.

Entry information

Entry name

RM10_YEAST

Accession

Primary (citable) accession number: P36520
Secondary accession number(s): D6W0R0, P36524

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program