true1994-06-012023-11-08139UDB15_RATCloning and expression of a rat liver phenobarbital-inducible UDP-glucuronosyltransferase (2B12) with specificity for monoterpenoid alcohols.Green M.D.Clarke D.J.Oturu E.M.Styczynski P.B.Jackson M.R.Burchell B.Tephly T.R.doi:10.1006/abbi.1995.14891995Arch. Biochem. Biophys.322460-468NUCLEOTIDE SEQUENCE [MRNA]FUNCTIONCATALYTIC ACTIVITYBIOPHYSICOCHEMICAL PROPERTIESTISSUE SPECIFICITYINDUCTIONSprague-DawleyLiverGreen M.D.1994-02EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [MRNA]KidneyPurification and properties of a rat liver phenobarbital-inducible 4-hydroxybiphenyl UDP-glucuronosyltransferase.Styczynski P.B.Green M.S.Puig J.Coffman B.L.Tephly T.R.1991Mol. Pharmacol.4080-84PROTEIN SEQUENCE OF 24-38CHARACTERIZATIONWistar1Glycosyltransferase Family 11 site, No reported glycans1 siteratUgt2b36Eukaryota5301GlucuronidationAspirin ADMEParacetamol ADMEPrednisone ADMEGT1_Gtf-likeGlycogen Phosphorylase BUDP_glucos_transUDP_glycos_trans_CSEG:EG0003.4 PROTEIN-RELATEDUDP-GLUCURONOSYLTRANSFERASEUDPGTUDP-Glycosyltransferase/glycogen phosphorylaseUDPGTUDP-glucuronosyltransferase 2B15UDPGT 2B15UGT2B152.4.1.17UDP-glucuronosyltransferase 2B36UDPGT 2B36Ugt2b15Ugt2b12Ugt2b36Ugt2b4UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed:7574722). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed:7574722). Catalyzes the glucuronidation of endogenous steroid hormones such as androgens (testosterone, androsterone) and estrogens (estradiol, epiestradiol, estriol, catechol estrogens) (PubMed:7574722). Displays glucuronidation activity toward several classes of xenoblotic substrates, including phenolic compounds (eugenol, 4-nitrophenol, 4-hydroxybiphenyl) and phenylpropanoids (naringenin, coumarins) (PubMed:7574722). Catalyzes the glucuronidation of monoterpenoid alcohols such as borneol, menthol and isomenthol, a class of natural compounds used in essential oils (PubMed:7574722).280 uM for UDP-alpha-D-glucuronate (when assaying glucuronidation with (1S,2R,4S)-borneol and 4-nitrophenol as substrates)330 uM for UDP-alpha-D-glucuronate (when assaying glucuronidation with (1S,2R,4S)-borneol and 4-nitrophenol as substrates)90 uM for 4-hydroxy-estrone140 uM for 4-hydroxy-estrone49 uM for naringenin55 uM for naringenin850 uM for 4-methylumbelliferone260 uM for eugenol1680 uM for 4-nitrophenol51 uM for 2-hydroxybiphenyl35 uM for 2-hydroxybiphenyl36 uM for (1S,2R,4S)-borneol10 uM for (+)-menthol50 uM for (+)-isomenthol585 uM for hexafluoro-2-propanol644 uM for hexafluoro-2-propanol140 pmol/min/mg enzyme for the formation of 4-hydroxy-estrone glucuronide190 pmol/min/mg enzyme for the formation of 4-hydroxy-estrone glucuronide117 pmol/min/mg enzyme for the formation of naringenin glucuronide123 pmol/min/mg enzyme for the formation of naringenin glucuronide137 pmol/min/mg enzyme for the formation of 4-methylumbelliferone glucuronide265 pmol/min/mg enzyme for the formation of eugenol glucuronide290 pmol/min/mg enzyme for the formation of 4-nitrophenol glucuronide220 pmol/min/mg enzyme for the formation of 2-hydroxybiphenyl glucuronide150 pmol/min/mg enzyme for the formation of 2-hydroxybiphenyl glucuronide320 pmol/min/mg enzyme for the formation of (1S,2R,4S)-borneol glucuronide9 pmol/min/mg enzyme for the formation of (+)-menthol glucuronide110 pmol/min/mg enzyme for the formation of (+)-isomenthol glucuronide280 pmol/min/mg enzyme for the formation of hexafluoro-2-propanol glucuronide330 pmol/min/mg enzyme for the formation of hexafluoro-2-propanol glucuronideLiver. Lower levels seen in the kidney and testis.By phenobarbital.N-glycosylated.The sequence shown is that of the liver isozyme. The kidney isoform differs in 12 positions.Belongs to the UDP-glycosyltransferase family.123UDP-glucuronosyltransferase 2B155853124530Helical494510N-linked (GlcNAc...) asparagine316In kidney.P2In kidney.S61In kidney.H71In kidney.S95In kidney.K183In kidney.T346In kidney.G398In kidney.AT414415In kidney.D433In kidney.L475In kidney.L4881994-06-011true610606ddecd4b841f3653813f4ed26cf1146dMSGKWISALLLLQISFCFKSGNCGKVLVWPMEYSHWMNIKIILEELVQKGHEVTVLRPSAFVFLDPKETSDLKFVTFPTSFSSHDLENFFTRFVNVWTYELPRDTCLSYFLYLQDTIDEYSDYCLTVCKEAVSNKQFMTKLQESKFDVVFSDAIGPCGELIAELLQIPFLYSLRFSPGYTIEQYIGGVLFPPSYVPMIFSGLAGQMTFIERVHNMICMLYFDFWFQTFREKKWDPFYSKTLGRPTTLAEIMGKAEMWLIRSYWDLEFPHPISPNVDYIGGLHCKPAKPLPKDIEDFVQSSGEHGVVVFSLGSMVRNMTEEKANIIAWALAQIPQKVLWRFDGKKPPTLGPNTRLYKWLPQNDLLGHPKTKAFVTHGGANGIYEAIHHGIPMIGIPLFAEQHDNIAHMVAKGAAVEVNFRTMSKSDLLNALEEVIDNPFYKKNAMWLSTIHHDQPTKPLDRAVFWIEFVMRHKGAKHLRSLGHNLPWYQYHSLDVIGFLLSCVAVTVVLALKCFLFVYRFFVKKEKKTKNEtruetruetruetruetruetruetruetruetruetrue