Reviewed, UniProtKB/Swiss-Prot P36511 (UD2B4_RAT)

Names and origin

Protein names

Recommended name:
    UDP-glucuronosyltransferase 2B4
      Short name=UDPGT 2B4
    EC=2.4.1.17
Alternative name(s):
    UDP-glucuronosyltransferase 2B12

Gene names

Name:	Ugt2b4
Synonyms:	Ugt2b12

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Catalyzes the transfer of glucuronic acid from UDP-glucuronic acid to various aglycone molecules. Catalyzes the glucuronidation of monoterpenoid alcohols, such as (-)-borneol, (+)-menthol, and (-)- nopol. In addition, a number of simple phenolic compounds, such as hydroxybiphenyls, 7-hydroxylated coumarins, p-nitrophenol, and food-derived substances (e.g., naringenin and eugenol), and 4-methylumbelliferone are also substrates.
Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.
Subcellular location	Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Tissue specificity	Liver. Lower levels seen in the kidney and testis.
Induction	By phenobarbital.
Post-translational modification	N-glycosylated Probable.
Polymorphism	The sequence shown is that of the liver isozyme. The kidney isoform differs in 12 positions.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Signal Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucuronosyltransferase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.2
	Chain	24 – 530	507	UDP-glucuronosyltransferase 2B4		PRO_0000036036
Regions
	Transmembrane	494 – 510	17	Potential
Amino acid modifications
	Glycosylation	316	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	2	1	S → P in kidney.
	Natural variant	61	1	F → S in kidney.
	Natural variant	71	1	D → H in kidney.
	Natural variant	95	1	N → S in kidney.
	Natural variant	183	1	Q → K in kidney.
	Natural variant	346	1	P → T in kidney.
	Natural variant	398	1	A → G in kidney.
	Natural variant	414 – 415	2	VE → AT in kidney.
	Natural variant	433	1	V → D in kidney.
	Natural variant	475	1	K → L in kidney.
	Natural variant	488	1	Q → L in kidney.

Sequences

	Sequence		Length	Mass (Da)
	P36511-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: D49313CE3E6D5BFD	FASTA	530	61,060
	10 20 30 40 50 60 MSGKWISALL LLQISFCFKS GNCGKVLVWP MEYSHWMNIK IILEELVQKG HEVTVLRPSA 70 80 90 100 110 120 FVFLDPKETS DLKFVTFPTS FSSHDLENFF TRFVNVWTYE LPRDTCLSYF LYLQDTIDEY 130 140 150 160 170 180 SDYCLTVCKE AVSNKQFMTK LQESKFDVVF SDAIGPCGEL IAELLQIPFL YSLRFSPGYT 190 200 210 220 230 240 IEQYIGGVLF PPSYVPMIFS GLAGQMTFIE RVHNMICMLY FDFWFQTFRE KKWDPFYSKT 250 260 270 280 290 300 LGRPTTLAEI MGKAEMWLIR SYWDLEFPHP ISPNVDYIGG LHCKPAKPLP KDIEDFVQSS 310 320 330 340 350 360 GEHGVVVFSL GSMVRNMTEE KANIIAWALA QIPQKVLWRF DGKKPPTLGP NTRLYKWLPQ 370 380 390 400 410 420 NDLLGHPKTK AFVTHGGANG IYEAIHHGIP MIGIPLFAEQ HDNIAHMVAK GAAVEVNFRT 430 440 450 460 470 480 MSKSDLLNAL EEVIDNPFYK KNAMWLSTIH HDQPTKPLDR AVFWIEFVMR HKGAKHLRSL 490 500 510 520 530 GHNLPWYQYH SLDVIGFLLS CVAVTVVLAL KCFLFVYRFF VKKEKKTKNE

References

[1]	"Cloning and expression of a rat liver phenobarbital-inducible UDP-glucuronosyltransferase (2B12) with specificity for monoterpenoid alcohols." Green M.D., Clarke D.J., Oturu E.M., Styczynski P.B., Jackson M.R., Burchell B., Tephly T.R. Arch. Biochem. Biophys. 322:460-468(1995) [PubMed: 7574722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney and Liver.
[2]	"Purification and properties of a rat liver phenobarbital-inducible 4-hydroxybiphenyl UDP-glucuronosyltransferase." Styczynski P.B., Green M.S., Puig J., Coffman B.L., Tephly T.R. Mol. Pharmacol. 40:80-84(1991) [PubMed: 1906977] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-38, CHARACTERIZATION. Strain: Wistar. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U06273 mRNA. Translation: AAA83404.1. U06274 mRNA. Translation: AAA83405.1.
IPI	IPI00203473.
PIR	S68200.
UniGene	Rn.3686
3D structure databases
SMR	P36511. Positions 286-447.
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PRIDE	P36511.
Genome annotation databases
Ensembl	ENSRNOG00000001980. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	620895. Ugt2b4.
Phylogenomic databases
HOVERGEN	P36511.
Enzyme and pathway databases
BRENDA	2.4.1.17. 248.
Gene expression databases
ArrayExpress	P36511.
GermOnline	ENSRNOG00000001980. Rattus norvegicus.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UD2B4_RAT

Accession

Primary (citable) accession number: P36511

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)