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Protein

UDP-glucuronosyltransferase 2B15

Gene

Ugt2b15

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Catalyzes the transfer of glucuronic acid from UDP-glucuronic acid to various aglycone molecules. Catalyzes the glucuronidation of monoterpenoid alcohols, such as (-)-borneol, (+)-menthol, and (-)- nopol. In addition, a number of simple phenolic compounds, such as hydroxybiphenyls, 7-hydroxylated coumarins, p-nitrophenol, and food-derived substances (e.g. naringenin and eugenol), and 4-methylumbelliferone are also substrates.

Catalytic activityi

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

GO - Molecular functioni

  1. glucuronosyltransferase activity Source: RGD

GO - Biological processi

  1. aromatic compound catabolic process Source: RGD
  2. cellular response to hormone stimulus Source: GO_Central
  3. flavonoid biosynthetic process Source: GO_Central
  4. flavonoid glucuronidation Source: GO_Central
  5. response to drug Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.17. 5301.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucuronosyltransferase 2B15 (EC:2.4.1.17)
Short name:
UDPGT 2B15
Alternative name(s):
UDP-glucuronosyltransferase 2B36
Short name:
UDPGT 2B36
Gene namesi
Name:Ugt2b15
Synonyms:Ugt2b12, Ugt2b36, Ugt2b4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620895. Ugt2b36.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei494 – 51017HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 530507UDP-glucuronosyltransferase 2B15PRO_0000036036Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.Curated

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP36511.
PRIDEiP36511.

Expressioni

Tissue specificityi

Liver. Lower levels seen in the kidney and testis.

Inductioni

By phenobarbital.

Gene expression databases

GenevestigatoriP36511.

Interactioni

Protein-protein interaction databases

MINTiMINT-4565295.
STRINGi10116.ENSRNOP00000002712.

Structurei

3D structure databases

ProteinModelPortaliP36511.
SMRiP36511. Positions 286-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1819.
HOVERGENiHBG004033.
InParanoidiP36511.
PhylomeDBiP36511.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36511-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGKWISALL LLQISFCFKS GNCGKVLVWP MEYSHWMNIK IILEELVQKG
60 70 80 90 100
HEVTVLRPSA FVFLDPKETS DLKFVTFPTS FSSHDLENFF TRFVNVWTYE
110 120 130 140 150
LPRDTCLSYF LYLQDTIDEY SDYCLTVCKE AVSNKQFMTK LQESKFDVVF
160 170 180 190 200
SDAIGPCGEL IAELLQIPFL YSLRFSPGYT IEQYIGGVLF PPSYVPMIFS
210 220 230 240 250
GLAGQMTFIE RVHNMICMLY FDFWFQTFRE KKWDPFYSKT LGRPTTLAEI
260 270 280 290 300
MGKAEMWLIR SYWDLEFPHP ISPNVDYIGG LHCKPAKPLP KDIEDFVQSS
310 320 330 340 350
GEHGVVVFSL GSMVRNMTEE KANIIAWALA QIPQKVLWRF DGKKPPTLGP
360 370 380 390 400
NTRLYKWLPQ NDLLGHPKTK AFVTHGGANG IYEAIHHGIP MIGIPLFAEQ
410 420 430 440 450
HDNIAHMVAK GAAVEVNFRT MSKSDLLNAL EEVIDNPFYK KNAMWLSTIH
460 470 480 490 500
HDQPTKPLDR AVFWIEFVMR HKGAKHLRSL GHNLPWYQYH SLDVIGFLLS
510 520 530
CVAVTVVLAL KCFLFVYRFF VKKEKKTKNE
Length:530
Mass (Da):61,060
Last modified:June 1, 1994 - v1
Checksum:iD49313CE3E6D5BFD
GO

Polymorphismi

The sequence shown is that of the liver isozyme. The kidney isoform differs in 12 positions.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti2 – 21S → P in kidney.
Natural varianti61 – 611F → S in kidney.
Natural varianti71 – 711D → H in kidney.
Natural varianti95 – 951N → S in kidney.
Natural varianti183 – 1831Q → K in kidney.
Natural varianti346 – 3461P → T in kidney.
Natural varianti398 – 3981A → G in kidney.
Natural varianti414 – 4152VE → AT in kidney.
Natural varianti433 – 4331V → D in kidney.
Natural varianti475 – 4751K → L in kidney.
Natural varianti488 – 4881Q → L in kidney.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06273 mRNA. Translation: AAA83404.1.
U06274 mRNA. Translation: AAA83405.1.
PIRiS68200.
UniGeneiRn.3686.

Genome annotation databases

UCSCiRGD:620895. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06273 mRNA. Translation: AAA83404.1.
U06274 mRNA. Translation: AAA83405.1.
PIRiS68200.
UniGeneiRn.3686.

3D structure databases

ProteinModelPortaliP36511.
SMRiP36511. Positions 286-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4565295.
STRINGi10116.ENSRNOP00000002712.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbiP36511.
PRIDEiP36511.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620895. rat.

Organism-specific databases

RGDi620895. Ugt2b36.

Phylogenomic databases

eggNOGiCOG1819.
HOVERGENiHBG004033.
InParanoidiP36511.
PhylomeDBiP36511.

Enzyme and pathway databases

BRENDAi2.4.1.17. 5301.

Gene expression databases

GenevestigatoriP36511.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of a rat liver phenobarbital-inducible UDP-glucuronosyltransferase (2B12) with specificity for monoterpenoid alcohols."
    Green M.D., Clarke D.J., Oturu E.M., Styczynski P.B., Jackson M.R., Burchell B., Tephly T.R.
    Arch. Biochem. Biophys. 322:460-468(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney and Liver.
  2. "Purification and properties of a rat liver phenobarbital-inducible 4-hydroxybiphenyl UDP-glucuronosyltransferase."
    Styczynski P.B., Green M.S., Puig J., Coffman B.L., Tephly T.R.
    Mol. Pharmacol. 40:80-84(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-38, CHARACTERIZATION.
    Strain: Wistar.
    Tissue: Liver.

Entry informationi

Entry nameiUDB15_RAT
AccessioniPrimary (citable) accession number: P36511
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.