Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P36506

- MP2K2_RAT

UniProt

P36506 - MP2K2_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Dual specificity mitogen-activated protein kinase kinase 2

Gene

Map2k2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 112Cleavage; by anthrax lethal factorBy similarity
Binding sitei101 – 1011ATPPROSITE-ProRule annotation
Active sitei194 – 1941Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 869ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. MAP kinase kinase activity Source: RGD
  3. protein serine/threonine/tyrosine kinase activity Source: UniProtKB
  4. protein serine/threonine kinase activator activity Source: Ensembl
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. protein tyrosine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. activation of MAPK activity Source: UniProtKB
  2. MAPK cascade Source: RGD
  3. peptidyl-serine autophosphorylation Source: Ensembl
  4. positive regulation of cell motility Source: Ensembl
  5. protein phosphorylation Source: RGD
  6. regulation of early endosome to late endosome transport Source: UniProtKB
  7. regulation of Golgi inheritance Source: UniProtKB
  8. regulation of stress-activated MAPK cascade Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2. 5301.
ReactomeiREACT_222166. Signaling by FGFR.
REACT_229643. MEK activation.
REACT_230341. Signal transduction by L1.
REACT_240952. ERK2 activation.
REACT_240957. RAF phosphorylates MEK.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity mitogen-activated protein kinase kinase 2 (EC:2.7.12.2)
Short name:
MAP kinase kinase 2
Short name:
MAPKK 2
Alternative name(s):
ERK activator kinase 2
MAPK/ERK kinase 2
Short name:
MEK 2
Gene namesi
Name:Map2k2
Synonyms:Mek2, Mkk2, Prkmk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi61888. Map2k2.

Subcellular locationi

GO - Cellular componenti

  1. cell-cell junction Source: Ensembl
  2. cell cortex Source: RGD
  3. cytoplasmic side of plasma membrane Source: Ensembl
  4. cytosol Source: UniProtKB
  5. early endosome Source: UniProtKB
  6. endoplasmic reticulum Source: Ensembl
  7. focal adhesion Source: UniProtKB
  8. Golgi apparatus Source: UniProtKB
  9. late endosome Source: UniProtKB
  10. microtubule Source: Ensembl
  11. mitochondrion Source: UniProtKB
  12. nucleus Source: UniProtKB
  13. perinuclear region of cytoplasm Source: Ensembl
  14. peroxisomal membrane Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Dual specificity mitogen-activated protein kinase kinase 2PRO_0000086374Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei222 – 2221Phosphoserine; by RAFBy similarity
Modified residuei226 – 2261Phosphoserine; by RAFBy similarity
Modified residuei293 – 2931PhosphoserineBy similarity
Modified residuei295 – 2951PhosphoserineBy similarity
Modified residuei394 – 3941PhosphothreonineBy similarity
Modified residuei396 – 3961PhosphothreonineBy similarity

Post-translational modificationi

MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP36506.
PRIDEiP36506.

PTM databases

PhosphoSiteiP36506.

Expressioni

Tissue specificityi

Expressed abundantly in the adult brain and muscle.

Gene expression databases

GenevestigatoriP36506.

Interactioni

Subunit structurei

Interacts with MORG1. Interacts with SGK1.By similarity

Protein-protein interaction databases

BioGridi248693. 7 interactions.
IntActiP36506. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP36506.
SMRiP36506. Positions 60-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 369298Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi266 – 31550Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
HOVERGENiHBG108518.
InParanoidiP36506.
KOiK04369.
OMAiRLKQPST.
OrthoDBiEOG7HF1KZ.
PhylomeDBiP36506.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36506-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLARRKPVLP ALTINPTIAE GPSPTSEGAS EAHLVDLQKK LEELDLDEQQ
60 70 80 90 100
RKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR
110 120 130 140 150
KLIHLEIKPA VRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM
160 170 180 190 200
DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI
210 220 230 240 250
LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI
260 270 280 290 300
WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP
310 320 330 340 350
PGRPISGHGM DSRPAMAIFE LLDYIVNEPP PKLPSGVFSS DFQEFVNKCL
360 370 380 390 400
IKNPAERADL KLLTNHAFIK RSEGEDVDFA GWLCRTLRLK QPSTPTRTAV
Length:400
Mass (Da):44,282
Last modified:June 1, 1994 - v1
Checksum:iB4E6AB15DEAA82A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14592 mRNA. Translation: BAA03442.1.
L14936 mRNA. Translation: AAA41620.1.
BC126084 mRNA. Translation: AAI26085.1.
PIRiA48081.
RefSeqiNP_579817.1. NM_133283.1.
UniGeneiRn.82693.

Genome annotation databases

EnsembliENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005.
GeneIDi58960.
KEGGirno:58960.
UCSCiRGD:61888. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14592 mRNA. Translation: BAA03442.1 .
L14936 mRNA. Translation: AAA41620.1 .
BC126084 mRNA. Translation: AAI26085.1 .
PIRi A48081.
RefSeqi NP_579817.1. NM_133283.1.
UniGenei Rn.82693.

3D structure databases

ProteinModelPortali P36506.
SMRi P36506. Positions 60-392.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248693. 7 interactions.
IntActi P36506. 1 interaction.

PTM databases

PhosphoSitei P36506.

Proteomic databases

PaxDbi P36506.
PRIDEi P36506.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027272 ; ENSRNOP00000027272 ; ENSRNOG00000020005 .
GeneIDi 58960.
KEGGi rno:58960.
UCSCi RGD:61888. rat.

Organism-specific databases

CTDi 5605.
RGDi 61888. Map2k2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119199.
HOGENOMi HOG000234206.
HOVERGENi HBG108518.
InParanoidi P36506.
KOi K04369.
OMAi RLKQPST.
OrthoDBi EOG7HF1KZ.
PhylomeDBi P36506.

Enzyme and pathway databases

BRENDAi 2.7.12.2. 5301.
Reactomei REACT_222166. Signaling by FGFR.
REACT_229643. MEK activation.
REACT_230341. Signal transduction by L1.
REACT_240952. ERK2 activation.
REACT_240957. RAF phosphorylates MEK.

Miscellaneous databases

NextBioi 611596.
PROi P36506.

Gene expression databases

Genevestigatori P36506.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of two members of the rat MAP kinase kinase gene family."
    Otsu M., Terada Y., Okayama H.
    FEBS Lett. 320:246-250(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Erratum
    Otsu M., Terada Y., Okayama H.
    FEBS Lett. 331:307-307(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2."
    Wu J., Harrison J.K., Dent P., Lynch K.R., Weber M.J., Sturgill T.W.
    Mol. Cell. Biol. 13:4539-4548(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiMP2K2_RAT
AccessioniPrimary (citable) accession number: P36506
Secondary accession number(s): A0JN15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3