Reviewed, UniProtKB/Swiss-Prot P36506 (MP2K2_RAT)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 2
      Short name=MAP kinase kinase 2
      Short name=MAPKK 2
    EC=2.7.12.2
Alternative name(s):
    ERK activator kinase 2
    MAPK/ERK kinase 2
      Short name=MEK 2

Gene names

Name:	Map2k2
Synonyms:	Mek2, Mkk2, Prkmk2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with MORG1 By similarity.
Tissue specificity	Expressed abundantly in the adult brain and muscle.
Post-translational modification	MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	MAPKKK cascade Inferred from mutant phenotype. Source: RGD protein amino acid phosphorylation Ref.3 Inferred from direct assay. Source: RGD
Cellular component	cell cortex Inferred from direct assay. Source: RGD cytosol Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from direct assay. Source: RGD MAP kinase kinase activity Ref.3 Inferred from direct assay. Source: RGD protein binding Inferred from physical interaction. Source: RGD protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Dual specificity mitogen-activated protein kinase kinase 2		PRO_0000086374
Regions
	Domain	72 – 369	298	Protein kinase
	Nucleotide binding	78 – 86	9	ATP By similarity
	Compositional bias	266 – 315	50	Pro-rich
Sites
	Active site	194	1	Proton acceptor By similarity
	Binding site	101	1	ATP By similarity
	Site	10 – 11	2	Cleavage; by anthrax lethal factor By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	23	1	Phosphoserine By similarity
	Modified residue	222	1	Phosphoserine; by RAF By similarity
	Modified residue	226	1	Phosphoserine; by RAF By similarity
	Modified residue	293	1	Phosphoserine By similarity
	Modified residue	295	1	Phosphoserine By similarity
	Modified residue	394	1	Phosphothreonine By similarity
	Modified residue	396	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36506-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: B4E6AB15DEAA82A4	FASTA	400	44,282
	10 20 30 40 50 60 MLARRKPVLP ALTINPTIAE GPSPTSEGAS EAHLVDLQKK LEELDLDEQQ RKRLEAFLTQ 70 80 90 100 110 120 KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR KLIHLEIKPA VRNQIIRELQ 130 140 150 160 170 180 VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG 190 200 210 220 230 240 LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ 250 260 270 280 290 300 GTHYSVQSDI WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP 310 320 330 340 350 360 PGRPISGHGM DSRPAMAIFE LLDYIVNEPP PKLPSGVFSS DFQEFVNKCL IKNPAERADL 370 380 390 400 KLLTNHAFIK RSEGEDVDFA GWLCRTLRLK QPSTPTRTAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of two members of the rat MAP kinase kinase gene family." Otsu M., Terada Y., Okayama H. FEBS Lett. 320:246-250(1993) [PubMed: 8462694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Erratum Otsu M., Terada Y., Okayama H. FEBS Lett. 331:307-307(1993) [PubMed: 8397117] [Abstract]
[3]	"Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2." Wu J., Harrison J.K., Dent P., Lynch K.R., Weber M.J., Sturgill T.W. Mol. Cell. Biol. 13:4539-4548(1993) [PubMed: 8393135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14592 mRNA. Translation: BAA03442.1. L14936 mRNA. Translation: AAA41620.1. BC126084 mRNA. Translation: AAI26085.1.
IPI	IPI00231331.
PIR	A48081.
RefSeq	NP_579817.1.
UniGene	Rn.82693
3D structure databases
SMR	P36506. Positions 60-392.
ModBase	Search...
Protein-protein interaction databases
STRING	P36506.
PTM databases
PhosphoSite	P36506.
Genome annotation databases
Ensembl	ENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005; Rattus norvegicus. [Genome view]
GeneID	58960.
KEGG	rno:58960.
UCSC	NM_133283. rat.
Organism-specific databases
CTD	58960.
RGD	61888. Map2k2.
Phylogenomic databases
eggNOG	roNOG05418.
HOVERGEN	P36506.
InParanoid	P36506.
PhylomeDB	P36506.
Enzyme and pathway databases
BRENDA	2.7.12.2. 248.
Gene expression databases
ArrayExpress	P36506.
Genevestigator	P36506.
GermOnline	ENSRNOG00000020005. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	611596.

Entry information

Entry name

MP2K2_RAT

Accession

Primary (citable) accession number: P36506
Secondary accession number(s): A0JN15

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	February 9, 2010
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)