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P36506 (MP2K2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity mitogen-activated protein kinase kinase 2

Short name=MAP kinase kinase 2
Short name=MAPKK 2
EC=2.7.12.2
Alternative name(s):
ERK activator kinase 2
MAPK/ERK kinase 2
Short name=MEK 2
Gene names
Name:Map2k2
Synonyms:Mek2, Mkk2, Prkmk2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with MORG1 By similarity. Interacts with SGK1 By similarity.

Tissue specificity

Expressed abundantly in the adult brain and muscle.

Post-translational modification

MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
Tyrosine-protein kinase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processMAPK cascade

Inferred from mutant phenotype PubMed 17310240. Source: RGD

activation of MAPK activity

Traceable author statement PubMed 19565474. Source: UniProtKB

peptidyl-serine autophosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell motility

Inferred from electronic annotation. Source: Ensembl

protein phosphorylation

Inferred from direct assay PubMed 7611406Ref.3. Source: RGD

regulation of Golgi inheritance

Traceable author statement PubMed 19565474. Source: UniProtKB

regulation of early endosome to late endosome transport

Traceable author statement PubMed 19565474. Source: UniProtKB

regulation of stress-activated MAPK cascade

Traceable author statement PubMed 19565474. Source: UniProtKB

   Cellular_componentGolgi apparatus

Traceable author statement PubMed 19565474. Source: UniProtKB

cell cortex

Inferred from direct assay PubMed 16272159. Source: RGD

cell-cell junction

Inferred from electronic annotation. Source: Ensembl

cytoplasmic side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

cytosol

Traceable author statement PubMed 19565474. Source: UniProtKB

early endosome

Traceable author statement PubMed 19565474. Source: UniProtKB

endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

focal adhesion

Traceable author statement PubMed 19565474. Source: UniProtKB

late endosome

Traceable author statement PubMed 19565474. Source: UniProtKB

microtubule

Inferred from electronic annotation. Source: Ensembl

mitochondrion

Traceable author statement PubMed 19565474. Source: UniProtKB

nucleus

Traceable author statement PubMed 19565474. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

peroxisomal membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionATP binding

Inferred from direct assay PubMed 7611406. Source: RGD

MAP kinase kinase activity

Inferred from direct assay PubMed 7611406Ref.3. Source: RGD

protein binding

Inferred from physical interaction PubMed 16272159. Source: RGD

protein serine/threonine kinase activator activity

Inferred from electronic annotation. Source: Ensembl

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine/tyrosine kinase activity

Traceable author statement PubMed 19565474. Source: UniProtKB

protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Dual specificity mitogen-activated protein kinase kinase 2
PRO_0000086374

Regions

Domain72 – 369298Protein kinase
Nucleotide binding78 – 869ATP By similarity
Compositional bias266 – 31550Pro-rich

Sites

Active site1941Proton acceptor By similarity
Binding site1011ATP By similarity
Site10 – 112Cleavage; by anthrax lethal factor By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue2221Phosphoserine; by RAF By similarity
Modified residue2261Phosphoserine; by RAF By similarity
Modified residue2931Phosphoserine By similarity
Modified residue2951Phosphoserine By similarity
Modified residue3941Phosphothreonine By similarity
Modified residue3961Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
P36506 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: B4E6AB15DEAA82A4

FASTA40044,282
        10         20         30         40         50         60 
MLARRKPVLP ALTINPTIAE GPSPTSEGAS EAHLVDLQKK LEELDLDEQQ RKRLEAFLTQ 

        70         80         90        100        110        120 
KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR KLIHLEIKPA VRNQIIRELQ 

       130        140        150        160        170        180 
VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG 

       190        200        210        220        230        240 
LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ 

       250        260        270        280        290        300 
GTHYSVQSDI WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP 

       310        320        330        340        350        360 
PGRPISGHGM DSRPAMAIFE LLDYIVNEPP PKLPSGVFSS DFQEFVNKCL IKNPAERADL 

       370        380        390        400 
KLLTNHAFIK RSEGEDVDFA GWLCRTLRLK QPSTPTRTAV 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of two members of the rat MAP kinase kinase gene family."
Otsu M., Terada Y., Okayama H.
FEBS Lett. 320:246-250(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Erratum
Otsu M., Terada Y., Okayama H.
FEBS Lett. 331:307-307(1993) [PubMed] [Europe PMC] [Abstract]
[3]"Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2."
Wu J., Harrison J.K., Dent P., Lynch K.R., Weber M.J., Sturgill T.W.
Mol. Cell. Biol. 13:4539-4548(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D14592 mRNA. Translation: BAA03442.1.
L14936 mRNA. Translation: AAA41620.1.
BC126084 mRNA. Translation: AAI26085.1.
PIRA48081.
RefSeqNP_579817.1. NM_133283.1.
UniGeneRn.82693.

3D structure databases

ProteinModelPortalP36506.
SMRP36506. Positions 60-392.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248693. 7 interactions.
IntActP36506. 1 interaction.

PTM databases

PhosphoSiteP36506.

Proteomic databases

PaxDbP36506.
PRIDEP36506.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005.
GeneID58960.
KEGGrno:58960.
UCSCRGD:61888. rat.

Organism-specific databases

CTD5605.
RGD61888. Map2k2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00690000101918.
HOGENOMHOG000234206.
HOVERGENHBG108518.
InParanoidP36506.
KOK04369.
OMARLKQPST.
OrthoDBEOG7HF1KZ.
PhylomeDBP36506.

Enzyme and pathway databases

BRENDA2.7.12.2. 5301.
ReactomeREACT_195021. Developmental Biology.
REACT_206767. Disease.
REACT_212996. Signal Transduction.
REACT_227097. Immune System.

Gene expression databases

GenevestigatorP36506.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio611596.
PROP36506.

Entry information

Entry nameMP2K2_RAT
AccessionPrimary (citable) accession number: P36506
Secondary accession number(s): A0JN15
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families