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Protein

Dual specificity mitogen-activated protein kinase kinase 2

Gene

Map2k2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101ATPPROSITE-ProRule annotation1
Active sitei194Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi78 – 86ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: RGD
  • MAP kinase kinase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine/tyrosine kinase activity Source: UniProtKB
  • protein tyrosine kinase activity Source: UniProtKB-KW
  • receptor signaling protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • activation of MAPK activity Source: UniProtKB
  • epithelial cell proliferation involved in lung morphogenesis Source: Ensembl
  • ERK1 and ERK2 cascade Source: Ensembl
  • face development Source: Ensembl
  • heart development Source: Ensembl
  • MAPK cascade Source: RGD
  • positive regulation of axonogenesis Source: Ensembl
  • positive regulation of cell motility Source: Ensembl
  • protein phosphorylation Source: RGD
  • regulation of axon regeneration Source: Ensembl
  • regulation of early endosome to late endosome transport Source: UniProtKB
  • regulation of Golgi inheritance Source: UniProtKB
  • regulation of stress-activated MAPK cascade Source: UniProtKB
  • thymus development Source: Ensembl
  • thyroid gland development Source: Ensembl
  • trachea formation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2. 5301.
ReactomeiR-RNO-112411. MAPK1 (ERK2) activation.
R-RNO-445144. Signal transduction by L1.
R-RNO-5673000. RAF activation.
R-RNO-5674135. MAP2K and MAPK activation.
R-RNO-5674499. Negative feedback regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity mitogen-activated protein kinase kinase 2 (EC:2.7.12.2By similarity)
Short name:
MAP kinase kinase 2
Short name:
MAPKK 2
Alternative name(s):
ERK activator kinase 2
MAPK/ERK kinase 2
Short name:
MEK 2
Gene namesi
Name:Map2k2
Synonyms:Mek2, Mkk2, Prkmk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi61888. Map2k2.

Subcellular locationi

  • Cytoplasm By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity

  • Note: Membrane localization is probably regulated by its interaction with KSR1.By similarity

GO - Cellular componenti

  • cell cortex Source: RGD
  • cytosol Source: UniProtKB
  • early endosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • late endosome Source: UniProtKB
  • membrane Source: UniProtKB-SubCell
  • mitochondrion Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863741 – 400Dual specificity mitogen-activated protein kinase kinase 2Add BLAST400

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei23PhosphoserineBy similarity1
Modified residuei222Phosphoserine; by RAFBy similarity1
Modified residuei226Phosphoserine; by RAFBy similarity1
Modified residuei293PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei306PhosphoserineBy similarity1
Modified residuei394PhosphothreonineBy similarity1
Modified residuei396PhosphothreonineBy similarity1

Post-translational modificationi

MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei10 – 11Cleavage; by anthrax lethal factorBy similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP36506.
PRIDEiP36506.

PTM databases

iPTMnetiP36506.
PhosphoSitePlusiP36506.

Expressioni

Tissue specificityi

Expressed abundantly in the adult brain and muscle.

Gene expression databases

BgeeiENSRNOG00000020005.
GenevisibleiP36506. RN.

Interactioni

Subunit structurei

Interacts with MORG1, SGK1 and KSR1.By similarity

Protein-protein interaction databases

BioGridi248693. 7 interactors.
IntActiP36506. 1 interactor.
STRINGi10116.ENSRNOP00000027272.

Structurei

3D structure databases

ProteinModelPortaliP36506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 369Protein kinasePROSITE-ProRule annotationAdd BLAST298

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi266 – 315Pro-richAdd BLAST50

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
HOVERGENiHBG108518.
InParanoidiP36506.
KOiK04369.
OMAiEGASEAH.
OrthoDBiEOG091G0DEC.
PhylomeDBiP36506.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLARRKPVLP ALTINPTIAE GPSPTSEGAS EAHLVDLQKK LEELDLDEQQ
60 70 80 90 100
RKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR
110 120 130 140 150
KLIHLEIKPA VRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM
160 170 180 190 200
DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI
210 220 230 240 250
LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI
260 270 280 290 300
WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP
310 320 330 340 350
PGRPISGHGM DSRPAMAIFE LLDYIVNEPP PKLPSGVFSS DFQEFVNKCL
360 370 380 390 400
IKNPAERADL KLLTNHAFIK RSEGEDVDFA GWLCRTLRLK QPSTPTRTAV
Length:400
Mass (Da):44,282
Last modified:June 1, 1994 - v1
Checksum:iB4E6AB15DEAA82A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14592 mRNA. Translation: BAA03442.1.
L14936 mRNA. Translation: AAA41620.1.
BC126084 mRNA. Translation: AAI26085.1.
PIRiA48081.
RefSeqiNP_579817.1. NM_133283.1.
UniGeneiRn.82693.

Genome annotation databases

EnsembliENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005.
GeneIDi58960.
KEGGirno:58960.
UCSCiRGD:61888. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14592 mRNA. Translation: BAA03442.1.
L14936 mRNA. Translation: AAA41620.1.
BC126084 mRNA. Translation: AAI26085.1.
PIRiA48081.
RefSeqiNP_579817.1. NM_133283.1.
UniGeneiRn.82693.

3D structure databases

ProteinModelPortaliP36506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248693. 7 interactors.
IntActiP36506. 1 interactor.
STRINGi10116.ENSRNOP00000027272.

PTM databases

iPTMnetiP36506.
PhosphoSitePlusiP36506.

Proteomic databases

PaxDbiP36506.
PRIDEiP36506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005.
GeneIDi58960.
KEGGirno:58960.
UCSCiRGD:61888. rat.

Organism-specific databases

CTDi5605.
RGDi61888. Map2k2.

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
HOVERGENiHBG108518.
InParanoidiP36506.
KOiK04369.
OMAiEGASEAH.
OrthoDBiEOG091G0DEC.
PhylomeDBiP36506.

Enzyme and pathway databases

BRENDAi2.7.12.2. 5301.
ReactomeiR-RNO-112411. MAPK1 (ERK2) activation.
R-RNO-445144. Signal transduction by L1.
R-RNO-5673000. RAF activation.
R-RNO-5674135. MAP2K and MAPK activation.
R-RNO-5674499. Negative feedback regulation of MAPK pathway.

Miscellaneous databases

PROiP36506.

Gene expression databases

BgeeiENSRNOG00000020005.
GenevisibleiP36506. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMP2K2_RAT
AccessioniPrimary (citable) accession number: P36506
Secondary accession number(s): A0JN15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.