Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P36506

- MP2K2_RAT

UniProt

P36506 - MP2K2_RAT

Protein

Dual specificity mitogen-activated protein kinase kinase 2

Gene

Map2k2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei10 – 112Cleavage; by anthrax lethal factorBy similarity
    Binding sitei101 – 1011ATPPROSITE-ProRule annotation
    Active sitei194 – 1941Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi78 – 869ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: RGD
    2. MAP kinase kinase activity Source: RGD
    3. protein binding Source: RGD
    4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB
    5. protein serine/threonine kinase activator activity Source: Ensembl
    6. protein serine/threonine kinase activity Source: UniProtKB-KW
    7. protein tyrosine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. activation of MAPK activity Source: UniProtKB
    2. MAPK cascade Source: RGD
    3. peptidyl-serine autophosphorylation Source: Ensembl
    4. positive regulation of cell motility Source: Ensembl
    5. protein phosphorylation Source: RGD
    6. regulation of early endosome to late endosome transport Source: UniProtKB
    7. regulation of Golgi inheritance Source: UniProtKB
    8. regulation of stress-activated MAPK cascade Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.12.2. 5301.
    ReactomeiREACT_222166. Signaling by FGFR.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 2 (EC:2.7.12.2)
    Short name:
    MAP kinase kinase 2
    Short name:
    MAPKK 2
    Alternative name(s):
    ERK activator kinase 2
    MAPK/ERK kinase 2
    Short name:
    MEK 2
    Gene namesi
    Name:Map2k2
    Synonyms:Mek2, Mkk2, Prkmk2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi61888. Map2k2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell-cell junction Source: Ensembl
    2. cell cortex Source: RGD
    3. cytoplasmic side of plasma membrane Source: Ensembl
    4. cytosol Source: UniProtKB
    5. early endosome Source: UniProtKB
    6. endoplasmic reticulum Source: Ensembl
    7. focal adhesion Source: UniProtKB
    8. Golgi apparatus Source: UniProtKB
    9. late endosome Source: UniProtKB
    10. microtubule Source: Ensembl
    11. mitochondrion Source: UniProtKB
    12. nucleus Source: UniProtKB
    13. perinuclear region of cytoplasm Source: Ensembl
    14. peroxisomal membrane Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400Dual specificity mitogen-activated protein kinase kinase 2PRO_0000086374Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei222 – 2221Phosphoserine; by RAFBy similarity
    Modified residuei226 – 2261Phosphoserine; by RAFBy similarity
    Modified residuei293 – 2931PhosphoserineBy similarity
    Modified residuei295 – 2951PhosphoserineBy similarity
    Modified residuei394 – 3941PhosphothreonineBy similarity
    Modified residuei396 – 3961PhosphothreonineBy similarity

    Post-translational modificationi

    MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP36506.
    PRIDEiP36506.

    PTM databases

    PhosphoSiteiP36506.

    Expressioni

    Tissue specificityi

    Expressed abundantly in the adult brain and muscle.

    Gene expression databases

    GenevestigatoriP36506.

    Interactioni

    Subunit structurei

    Interacts with MORG1. Interacts with SGK1.By similarity

    Protein-protein interaction databases

    BioGridi248693. 7 interactions.
    IntActiP36506. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP36506.
    SMRiP36506. Positions 60-392.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini72 – 369298Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi266 – 31550Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00690000101918.
    HOGENOMiHOG000234206.
    HOVERGENiHBG108518.
    InParanoidiP36506.
    KOiK04369.
    OMAiRLKQPST.
    OrthoDBiEOG7HF1KZ.
    PhylomeDBiP36506.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36506-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLARRKPVLP ALTINPTIAE GPSPTSEGAS EAHLVDLQKK LEELDLDEQQ    50
    RKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR 100
    KLIHLEIKPA VRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM 150
    DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI 200
    LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI 250
    WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP 300
    PGRPISGHGM DSRPAMAIFE LLDYIVNEPP PKLPSGVFSS DFQEFVNKCL 350
    IKNPAERADL KLLTNHAFIK RSEGEDVDFA GWLCRTLRLK QPSTPTRTAV 400
    Length:400
    Mass (Da):44,282
    Last modified:June 1, 1994 - v1
    Checksum:iB4E6AB15DEAA82A4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14592 mRNA. Translation: BAA03442.1.
    L14936 mRNA. Translation: AAA41620.1.
    BC126084 mRNA. Translation: AAI26085.1.
    PIRiA48081.
    RefSeqiNP_579817.1. NM_133283.1.
    UniGeneiRn.82693.

    Genome annotation databases

    EnsembliENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005.
    GeneIDi58960.
    KEGGirno:58960.
    UCSCiRGD:61888. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14592 mRNA. Translation: BAA03442.1 .
    L14936 mRNA. Translation: AAA41620.1 .
    BC126084 mRNA. Translation: AAI26085.1 .
    PIRi A48081.
    RefSeqi NP_579817.1. NM_133283.1.
    UniGenei Rn.82693.

    3D structure databases

    ProteinModelPortali P36506.
    SMRi P36506. Positions 60-392.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248693. 7 interactions.
    IntActi P36506. 1 interaction.

    PTM databases

    PhosphoSitei P36506.

    Proteomic databases

    PaxDbi P36506.
    PRIDEi P36506.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027272 ; ENSRNOP00000027272 ; ENSRNOG00000020005 .
    GeneIDi 58960.
    KEGGi rno:58960.
    UCSCi RGD:61888. rat.

    Organism-specific databases

    CTDi 5605.
    RGDi 61888. Map2k2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00690000101918.
    HOGENOMi HOG000234206.
    HOVERGENi HBG108518.
    InParanoidi P36506.
    KOi K04369.
    OMAi RLKQPST.
    OrthoDBi EOG7HF1KZ.
    PhylomeDBi P36506.

    Enzyme and pathway databases

    BRENDAi 2.7.12.2. 5301.
    Reactomei REACT_222166. Signaling by FGFR.

    Miscellaneous databases

    NextBioi 611596.
    PROi P36506.

    Gene expression databases

    Genevestigatori P36506.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of two members of the rat MAP kinase kinase gene family."
      Otsu M., Terada Y., Okayama H.
      FEBS Lett. 320:246-250(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Erratum
      Otsu M., Terada Y., Okayama H.
      FEBS Lett. 331:307-307(1993) [PubMed] [Europe PMC] [Abstract]
    3. "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2."
      Wu J., Harrison J.K., Dent P., Lynch K.R., Weber M.J., Sturgill T.W.
      Mol. Cell. Biol. 13:4539-4548(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiMP2K2_RAT
    AccessioniPrimary (citable) accession number: P36506
    Secondary accession number(s): A0JN15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3