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Protein

Dual specificity mitogen-activated protein kinase kinase 2

Gene

Map2k2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 112Cleavage; by anthrax lethal factorBy similarity
Binding sitei101 – 1011ATPPROSITE-ProRule annotation
Active sitei194 – 1941Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 869ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. MAP kinase kinase activity Source: RGD
  3. protein serine/threonine/tyrosine kinase activity Source: UniProtKB
  4. protein serine/threonine kinase activator activity Source: Ensembl
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. protein tyrosine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. activation of MAPK activity Source: UniProtKB
  2. MAPK cascade Source: RGD
  3. peptidyl-serine autophosphorylation Source: Ensembl
  4. positive regulation of cell motility Source: Ensembl
  5. protein phosphorylation Source: RGD
  6. regulation of early endosome to late endosome transport Source: UniProtKB
  7. regulation of Golgi inheritance Source: UniProtKB
  8. regulation of stress-activated MAPK cascade Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2. 5301.
ReactomeiREACT_272389. RAF phosphorylates MEK.
REACT_291588. Signaling by FGFR.
REACT_296554. Signal transduction by L1.
REACT_311446. MEK activation.
REACT_317180. ERK2 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity mitogen-activated protein kinase kinase 2 (EC:2.7.12.2)
Short name:
MAP kinase kinase 2
Short name:
MAPKK 2
Alternative name(s):
ERK activator kinase 2
MAPK/ERK kinase 2
Short name:
MEK 2
Gene namesi
Name:Map2k2
Synonyms:Mek2, Mkk2, Prkmk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi61888. Map2k2.

Subcellular locationi

GO - Cellular componenti

  1. cell-cell junction Source: Ensembl
  2. cell cortex Source: RGD
  3. cytoplasmic side of plasma membrane Source: Ensembl
  4. cytosol Source: UniProtKB
  5. early endosome Source: UniProtKB
  6. endoplasmic reticulum Source: Ensembl
  7. focal adhesion Source: UniProtKB
  8. Golgi apparatus Source: UniProtKB
  9. late endosome Source: UniProtKB
  10. microtubule Source: Ensembl
  11. mitochondrion Source: UniProtKB
  12. nucleus Source: UniProtKB
  13. perinuclear region of cytoplasm Source: Ensembl
  14. peroxisomal membrane Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Dual specificity mitogen-activated protein kinase kinase 2PRO_0000086374Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei23 – 231PhosphoserineBy similarity
Modified residuei222 – 2221Phosphoserine; by RAFBy similarity
Modified residuei226 – 2261Phosphoserine; by RAFBy similarity
Modified residuei293 – 2931PhosphoserineBy similarity
Modified residuei295 – 2951PhosphoserineBy similarity
Modified residuei394 – 3941PhosphothreonineBy similarity
Modified residuei396 – 3961PhosphothreonineBy similarity

Post-translational modificationi

MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP36506.
PRIDEiP36506.

PTM databases

PhosphoSiteiP36506.

Expressioni

Tissue specificityi

Expressed abundantly in the adult brain and muscle.

Gene expression databases

GenevestigatoriP36506.

Interactioni

Subunit structurei

Interacts with MORG1. Interacts with SGK1.By similarity

Protein-protein interaction databases

BioGridi248693. 7 interactions.
IntActiP36506. 1 interaction.

Structurei

3D structure databases

SMRiP36506. Positions 60-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 369298Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi266 – 31550Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
HOVERGENiHBG108518.
InParanoidiP36506.
KOiK04369.
OMAiEGASEAH.
OrthoDBiEOG7HF1KZ.
PhylomeDBiP36506.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLARRKPVLP ALTINPTIAE GPSPTSEGAS EAHLVDLQKK LEELDLDEQQ
60 70 80 90 100
RKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR
110 120 130 140 150
KLIHLEIKPA VRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM
160 170 180 190 200
DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI
210 220 230 240 250
LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI
260 270 280 290 300
WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP
310 320 330 340 350
PGRPISGHGM DSRPAMAIFE LLDYIVNEPP PKLPSGVFSS DFQEFVNKCL
360 370 380 390 400
IKNPAERADL KLLTNHAFIK RSEGEDVDFA GWLCRTLRLK QPSTPTRTAV
Length:400
Mass (Da):44,282
Last modified:June 1, 1994 - v1
Checksum:iB4E6AB15DEAA82A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14592 mRNA. Translation: BAA03442.1.
L14936 mRNA. Translation: AAA41620.1.
BC126084 mRNA. Translation: AAI26085.1.
PIRiA48081.
RefSeqiNP_579817.1. NM_133283.1.
UniGeneiRn.82693.

Genome annotation databases

EnsembliENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005.
GeneIDi58960.
KEGGirno:58960.
UCSCiRGD:61888. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14592 mRNA. Translation: BAA03442.1.
L14936 mRNA. Translation: AAA41620.1.
BC126084 mRNA. Translation: AAI26085.1.
PIRiA48081.
RefSeqiNP_579817.1. NM_133283.1.
UniGeneiRn.82693.

3D structure databases

SMRiP36506. Positions 60-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248693. 7 interactions.
IntActiP36506. 1 interaction.

PTM databases

PhosphoSiteiP36506.

Proteomic databases

PaxDbiP36506.
PRIDEiP36506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005.
GeneIDi58960.
KEGGirno:58960.
UCSCiRGD:61888. rat.

Organism-specific databases

CTDi5605.
RGDi61888. Map2k2.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
HOVERGENiHBG108518.
InParanoidiP36506.
KOiK04369.
OMAiEGASEAH.
OrthoDBiEOG7HF1KZ.
PhylomeDBiP36506.

Enzyme and pathway databases

BRENDAi2.7.12.2. 5301.
ReactomeiREACT_272389. RAF phosphorylates MEK.
REACT_291588. Signaling by FGFR.
REACT_296554. Signal transduction by L1.
REACT_311446. MEK activation.
REACT_317180. ERK2 activation.

Miscellaneous databases

NextBioi611596.
PROiP36506.

Gene expression databases

GenevestigatoriP36506.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of two members of the rat MAP kinase kinase gene family."
    Otsu M., Terada Y., Okayama H.
    FEBS Lett. 320:246-250(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Erratum
    Otsu M., Terada Y., Okayama H.
    FEBS Lett. 331:307-307(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2."
    Wu J., Harrison J.K., Dent P., Lynch K.R., Weber M.J., Sturgill T.W.
    Mol. Cell. Biol. 13:4539-4548(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiMP2K2_RAT
AccessioniPrimary (citable) accession number: P36506
Secondary accession number(s): A0JN15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 1, 2015
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.