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P36506

- MP2K2_RAT

UniProt

P36506 - MP2K2_RAT

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Protein
Dual specificity mitogen-activated protein kinase kinase 2
Gene
Map2k2, Mek2, Mkk2, Prkmk2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 112Cleavage; by anthrax lethal factor By similarity
Binding sitei101 – 1011ATP By similarity
Active sitei194 – 1941Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 869ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. MAP kinase kinase activity Source: RGD
  3. protein binding Source: RGD
  4. protein serine/threonine kinase activator activity Source: Ensembl
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. protein serine/threonine/tyrosine kinase activity Source: UniProtKB
  7. protein tyrosine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. MAPK cascade Source: RGD
  2. activation of MAPK activity Source: UniProtKB
  3. peptidyl-serine autophosphorylation Source: Ensembl
  4. positive regulation of cell motility Source: Ensembl
  5. protein phosphorylation Source: RGD
  6. regulation of Golgi inheritance Source: UniProtKB
  7. regulation of early endosome to late endosome transport Source: UniProtKB
  8. regulation of stress-activated MAPK cascade Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2. 5301.
ReactomeiREACT_222166. Signaling by FGFR.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity mitogen-activated protein kinase kinase 2 (EC:2.7.12.2)
Short name:
MAP kinase kinase 2
Short name:
MAPKK 2
Alternative name(s):
ERK activator kinase 2
MAPK/ERK kinase 2
Short name:
MEK 2
Gene namesi
Name:Map2k2
Synonyms:Mek2, Mkk2, Prkmk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi61888. Map2k2.

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. cell cortex Source: RGD
  3. cell-cell junction Source: Ensembl
  4. cytoplasmic side of plasma membrane Source: Ensembl
  5. cytosol Source: UniProtKB
  6. early endosome Source: UniProtKB
  7. endoplasmic reticulum Source: Ensembl
  8. focal adhesion Source: UniProtKB
  9. late endosome Source: UniProtKB
  10. microtubule Source: Ensembl
  11. mitochondrion Source: UniProtKB
  12. nucleus Source: UniProtKB
  13. perinuclear region of cytoplasm Source: Ensembl
  14. peroxisomal membrane Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Dual specificity mitogen-activated protein kinase kinase 2
PRO_0000086374Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei222 – 2221Phosphoserine; by RAF By similarity
Modified residuei226 – 2261Phosphoserine; by RAF By similarity
Modified residuei293 – 2931Phosphoserine By similarity
Modified residuei295 – 2951Phosphoserine By similarity
Modified residuei394 – 3941Phosphothreonine By similarity
Modified residuei396 – 3961Phosphothreonine By similarity

Post-translational modificationi

MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP36506.
PRIDEiP36506.

PTM databases

PhosphoSiteiP36506.

Expressioni

Tissue specificityi

Expressed abundantly in the adult brain and muscle.

Gene expression databases

GenevestigatoriP36506.

Interactioni

Subunit structurei

Interacts with MORG1 By similarity. Interacts with SGK1 By similarity.

Protein-protein interaction databases

BioGridi248693. 7 interactions.
IntActiP36506. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP36506.
SMRiP36506. Positions 60-392.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 369298Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi266 – 31550Pro-rich
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00690000101918.
HOGENOMiHOG000234206.
HOVERGENiHBG108518.
InParanoidiP36506.
KOiK04369.
OMAiRLKQPST.
OrthoDBiEOG7HF1KZ.
PhylomeDBiP36506.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36506-1 [UniParc]FASTAAdd to Basket

« Hide

MLARRKPVLP ALTINPTIAE GPSPTSEGAS EAHLVDLQKK LEELDLDEQQ    50
RKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR 100
KLIHLEIKPA VRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM 150
DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI 200
LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI 250
WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP 300
PGRPISGHGM DSRPAMAIFE LLDYIVNEPP PKLPSGVFSS DFQEFVNKCL 350
IKNPAERADL KLLTNHAFIK RSEGEDVDFA GWLCRTLRLK QPSTPTRTAV 400
Length:400
Mass (Da):44,282
Last modified:June 1, 1994 - v1
Checksum:iB4E6AB15DEAA82A4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14592 mRNA. Translation: BAA03442.1.
L14936 mRNA. Translation: AAA41620.1.
BC126084 mRNA. Translation: AAI26085.1.
PIRiA48081.
RefSeqiNP_579817.1. NM_133283.1.
UniGeneiRn.82693.

Genome annotation databases

EnsembliENSRNOT00000027272; ENSRNOP00000027272; ENSRNOG00000020005.
GeneIDi58960.
KEGGirno:58960.
UCSCiRGD:61888. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14592 mRNA. Translation: BAA03442.1 .
L14936 mRNA. Translation: AAA41620.1 .
BC126084 mRNA. Translation: AAI26085.1 .
PIRi A48081.
RefSeqi NP_579817.1. NM_133283.1.
UniGenei Rn.82693.

3D structure databases

ProteinModelPortali P36506.
SMRi P36506. Positions 60-392.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248693. 7 interactions.
IntActi P36506. 1 interaction.

PTM databases

PhosphoSitei P36506.

Proteomic databases

PaxDbi P36506.
PRIDEi P36506.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027272 ; ENSRNOP00000027272 ; ENSRNOG00000020005 .
GeneIDi 58960.
KEGGi rno:58960.
UCSCi RGD:61888. rat.

Organism-specific databases

CTDi 5605.
RGDi 61888. Map2k2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00690000101918.
HOGENOMi HOG000234206.
HOVERGENi HBG108518.
InParanoidi P36506.
KOi K04369.
OMAi RLKQPST.
OrthoDBi EOG7HF1KZ.
PhylomeDBi P36506.

Enzyme and pathway databases

BRENDAi 2.7.12.2. 5301.
Reactomei REACT_222166. Signaling by FGFR.

Miscellaneous databases

NextBioi 611596.
PROi P36506.

Gene expression databases

Genevestigatori P36506.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of two members of the rat MAP kinase kinase gene family."
    Otsu M., Terada Y., Okayama H.
    FEBS Lett. 320:246-250(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Erratum
    Otsu M., Terada Y., Okayama H.
    FEBS Lett. 331:307-307(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2."
    Wu J., Harrison J.K., Dent P., Lynch K.R., Weber M.J., Sturgill T.W.
    Mol. Cell. Biol. 13:4539-4548(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiMP2K2_RAT
AccessioniPrimary (citable) accession number: P36506
Secondary accession number(s): A0JN15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 3, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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