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Reviewed, UniProtKB/Swiss-Prot P36444 (MAOC_FLAPR)

Last modified June 16, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADP-dependent malic enzyme, chloroplastic
      Short name=NADP-ME
    EC=1.1.1.40
Gene names
Name: MODA
OrganismFlaveria pringlei
Taxonomic identifier4226 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Tageteae › Flaveria

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Protein attributes

Sequence length647 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO2 released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO2 that occurs in most plants.

Catalytic activity

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Pathway

Photosynthesis; C3 acid pathway.

Subunit structure

Homotetramer.

Subcellular location

Plastid › chloroplast.

Sequence similarities

Belongs to the malic enzymes family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?6161Chloroplast
Chain?62 – 647586NADP-dependent malic enzyme, chloroplastic
PRO_0000018545

Regions

Nucleotide binding391 – 40717NADP By similarity

Sites

Active site1951Proton donor By similarity
Active site2661Proton acceptor By similarity
Metal binding3381Divalent metal cation By similarity
Metal binding3391Divalent metal cation By similarity
Metal binding3621Divalent metal cation By similarity
Binding site2481NAD By similarity
Binding site3621NAD By similarity
Binding site5031NAD By similarity
Site3621Important for activity By similarity

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Sequences

Sequence LengthMass (Da)Tools
P36444-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: B06AE314F48BE352

FASTA64771,204
        10         20         30         40         50         60 
MMSLNSSSVV KSSISGVSWT QSQSVRLSVR RPMVVAMVNS NGRPERSVGV SVDGAVKDVN 

        70         80         90        100        110        120 
APVAVEVADS ESKKPTAVVG GGVEDVYGED SATEDHFITP WSVSVASGYS LLRDPHHNKG 

       130        140        150        160        170        180 
LAFTEKERDA HYLRGLLPPV VVNHDLQVKK MMHNIRQYEV PLQRYQAMMD LQERNERLFY 

       190        200        210        220        230        240 
KLLIENIEEL LPIVYTPTVG EACQKYGTIF KNPQGLYISL KDKGKVLEIL KNWPQKKIQV 

       250        260        270        280        290        300 
IVVTDGERIL GLGDLGCQGM GIPVGKLSLY TALGGIRPSA CLPITIDVGT NNEKMLNDEF 

       310        320        330        340        350        360 
YIGLRQRRAS GKEYAELMNE FMSAVKQNYG EKVLIQFEDF ANHNAFDLLE KYRTTHLVFN 

       370        380        390        400        410        420 
DDIQGTASVV LAGLISALKL VGGSLADHKF LFLGAGEAGT GIAELIALEI SKQTNAPLEE 

       430        440        450        460        470        480 
TRKKIWLVDS KGLIVRSRLD SLQHFKKPWA HDHEPVNKFL DAVKAIKPTV LIGSSGAGQT 

       490        500        510        520        530        540 
FTKEVVEAMS SFNEKPIILA LSNPTSQSEC TAEQAYTWSE GRTIFASGSP FAPVEYNGKV 

       550        560        570        580        590        600 
YVSGQSNNAY IFPGFGLGLI ISGAIRVHDE MLLAASEALA EQVTQEHFDN GLIYPPFTNI 

       610        620        630        640 
RKISAHIAAK VAAKAYELGL ASRLPQPENL VAYAESCMYS PKYRNYR

« Hide

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References

[1]"The C3 plant Flaveria pringlei contains a plastidic NADP-malic enzyme which is orthologous to the C4 isoform of the C4 plant F. trinervia."
Lipka B., Steinmueller K., Rosche E., Borsch D., Westhoff P.
Plant Mol. Biol. 26:1775-1783(1994) [PubMed: 7858216] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.

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Cross-references

Sequence databases

X78069 mRNA. Translation: CAA54986.1.
PIRS42939. S52016.

3D structure databases

HSSPHSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.1.1.40. 2732.

Family and domain databases

InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N.
IPR012302. Malic_NAD_bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PRINTSPR00072. MALOXRDTASE.
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMAOC_FLAPR
AccessionPrimary (citable) accession number: P36444
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents