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Protein

Cytochrome f

Gene

petA

Organism
Brassica rapa subsp. rapa (Turnip)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.By similarity

Cofactori

heme3 PublicationsNote: Binds 1 heme group covalently.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Iron (heme axial ligand); via amino nitrogen3 Publications
Binding sitei56 – 561Heme (covalent)3 Publications
Binding sitei59 – 591Heme (covalent)3 Publications
Metal bindingi60 – 601Iron (heme axial ligand)3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome f
Gene namesi
Name:petA
Encoded oniPlastid; Chloroplast
OrganismiBrassica rapa subsp. rapa (Turnip)
Taxonomic identifieri51350 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei286 – 30520HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 35351 PublicationAdd
BLAST
Chaini36 – 320285Cytochrome fPRO_0000023806Add
BLAST

Post-translational modificationi

Purified from leaves as a water-soluble monomeric protein with a mass of 28.16 kDa, cleavage occurs after Gln-287 and separates the heme-binding from the membrane.1 Publication

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-62N.
MINTiMINT-1534157.

Structurei

Secondary structure

1
320
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi37 – 437Combined sources
Beta strandi45 – 484Combined sources
Helixi55 – 584Combined sources
Beta strandi67 – 693Combined sources
Beta strandi72 – 743Combined sources
Beta strandi79 – 857Combined sources
Beta strandi99 – 1024Combined sources
Beta strandi105 – 1117Combined sources
Helixi121 – 1233Combined sources
Helixi126 – 1327Combined sources
Beta strandi138 – 1414Combined sources
Beta strandi147 – 1548Combined sources
Helixi155 – 1584Combined sources
Beta strandi159 – 1668Combined sources
Turni170 – 1723Combined sources
Beta strandi180 – 19011Combined sources
Beta strandi202 – 2043Combined sources
Beta strandi212 – 2198Combined sources
Beta strandi225 – 2317Combined sources
Turni232 – 2354Combined sources
Beta strandi236 – 2427Combined sources
Beta strandi261 – 2633Combined sources
Beta strandi270 – 28011Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CTMX-ray2.30A36-285[»]
1HCZX-ray1.96A36-287[»]
1JX8model-A36-285[»]
1TKWNMR-B36-287[»]
2PCFNMR-B36-285[»]
ProteinModelPortaliP36438.
SMRiP36438. Positions 36-320.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36438.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome f family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.5.700. 1 hit.
2.60.40.830. 2 hits.
HAMAPiMF_00610. Cytb6_f_cytF. 1 hit.
InterProiIPR024058. Cyt-f_TM.
IPR002325. Cyt_f.
IPR024094. Cyt_f_lg_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01333. Apocytochr_F_C. 1 hit.
PF16639. Apocytochr_F_N. 1 hit.
[Graphical view]
PRINTSiPR00610. CYTOCHROMEF.
SUPFAMiSSF103431. SSF103431. 1 hit.
SSF49441. SSF49441. 2 hits.
SSF51246. SSF51246. 1 hit.
PROSITEiPS51010. CYTF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36438-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTRNTFSWI REEITRSISV SLMIYIITWA SISSAYPIFA QQNYENPREA
60 70 80 90 100
TGRIVCANCH LASKPVDIEV PQAVLPDTVF EAVVKIPYDM QLKQVLANGK
110 120 130 140 150
KGALNVGAVL ILPEGFELAP PDRISPEMKE KIGNLSFQNY RPNKKNILVI
160 170 180 190 200
GPVPGQKYSE ITFPILAPDP ATNKDVHFLK YPIYVGGNRG RGQIYPDGSK
210 220 230 240 250
SNNTVYNATA GGIISKILRK EKGGYEITIV DASNERQVID IIPRGLELLV
260 270 280 290 300
SEGESIKLDQ PLTSNPNVGG FGQGDAEIVL QDPLRVQGLL FFLGSVVLAQ
310 320
IFLVLKKKQF EKVQLSEMNF
Length:320
Mass (Da):35,389
Last modified:June 1, 1994 - v1
Checksum:i466324C040C3E00F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77011 Genomic DNA. Translation: CAA54307.1.
PIRiS45661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77011 Genomic DNA. Translation: CAA54307.1.
PIRiS45661.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CTMX-ray2.30A36-285[»]
1HCZX-ray1.96A36-287[»]
1JX8model-A36-285[»]
1TKWNMR-B36-287[»]
2PCFNMR-B36-285[»]
ProteinModelPortaliP36438.
SMRiP36438. Positions 36-320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-62N.
MINTiMINT-1534157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP36438.

Family and domain databases

Gene3Di1.20.5.700. 1 hit.
2.60.40.830. 2 hits.
HAMAPiMF_00610. Cytb6_f_cytF. 1 hit.
InterProiIPR024058. Cyt-f_TM.
IPR002325. Cyt_f.
IPR024094. Cyt_f_lg_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01333. Apocytochr_F_C. 1 hit.
PF16639. Apocytochr_F_N. 1 hit.
[Graphical view]
PRINTSiPR00610. CYTOCHROMEF.
SUPFAMiSSF103431. SSF103431. 1 hit.
SSF49441. SSF49441. 2 hits.
SSF51246. SSF51246. 1 hit.
PROSITEiPS51010. CYTF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYF_BRARR
AccessioniPrimary (citable) accession number: P36438
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.