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Protein

Type-2 restriction enzyme HpaII

Gene

hpaIIR

Organism
Haemophilus parainfluenzae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence CCGG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1159. HpaII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HpaII (EC:3.1.21.4)
Short name:
R.HpaII
Alternative name(s):
Endonuclease HpaII
Type II restriction enzyme HpaII
Gene namesi
Name:hpaIIR
OrganismiHaemophilus parainfluenzae
Taxonomic identifieri729 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Type-2 restriction enzyme HpaIIPRO_0000077325Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Family & Domainsi

Family and domain databases

InterProiIPR019062. Restrct_endonuc_II_HpaII.
[Graphical view]
PfamiPF09561. RE_HpaII. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEFFSGNRG EWSEPYALFK LLADGQLYLG DSQLNKLGIV MPILSILRQE
60 70 80 90 100
KNYESSYILH NNSQNIIVTY NNEKFTVPIS GFQEKAVLLL SEIKNASGNR
110 120 130 140 150
AFSIPSIDDF LKKLGFTHLS ASSSSKSDIH IVVHDLRTGI TPTLGFSIKS
160 170 180 190 200
QLGSPATLLN ASKATNFTFK IYNLKDKQIE YINSLSGIKE KIKEIFSQDG
210 220 230 240 250
KLEFVKVESC KFSNNLTLID TKLPEILAEM ILLYYSSKLN KIDDVTEHIS
260 270 280 290 300
RLNPLNYNLS CNHNYYEYKV KHFLNDVALG MRPDDVWLGQ YDATGGYLVV
310 320 330 340 350
KEDGELLCYH IYSKNSFEDY LYCNTKFDTP SSSRHDFGHI YQVNHDFFIK

LNVQIRFL
Length:358
Mass (Da):40,926
Last modified:June 1, 1994 - v1
Checksum:iECB312180C80303E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17342 Genomic DNA. Translation: AAA20482.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17342 Genomic DNA. Translation: AAA20482.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1159. HpaII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019062. Restrct_endonuc_II_HpaII.
[Graphical view]
PfamiPF09561. RE_HpaII. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Organization and sequence of the HpaII restriction-modification system and adjacent genes."
    Kulakauskas S., Barsomian J.M., Lubys A., Roberts R.J., Wilson G.G.
    Gene 142:9-15(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49669.

Entry informationi

Entry nameiT2H2_HAEPA
AccessioniPrimary (citable) accession number: P36433
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.