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P36433 (T2H2_HAEPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HpaII

Short name=R.HpaII
EC=3.1.21.4
Alternative name(s):
Endonuclease HpaII
Type II restriction enzyme HpaII
Gene names
Name:hpaIIR
OrganismHaemophilus parainfluenzae
Taxonomic identifier729 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCGG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Subunit structure

Homodimer.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Type-2 restriction enzyme HpaII
PRO_0000077325

Sequences

Sequence LengthMass (Da)Tools
P36433 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: ECB312180C80303E

FASTA35840,926
        10         20         30         40         50         60 
MTEFFSGNRG EWSEPYALFK LLADGQLYLG DSQLNKLGIV MPILSILRQE KNYESSYILH 

        70         80         90        100        110        120 
NNSQNIIVTY NNEKFTVPIS GFQEKAVLLL SEIKNASGNR AFSIPSIDDF LKKLGFTHLS 

       130        140        150        160        170        180 
ASSSSKSDIH IVVHDLRTGI TPTLGFSIKS QLGSPATLLN ASKATNFTFK IYNLKDKQIE 

       190        200        210        220        230        240 
YINSLSGIKE KIKEIFSQDG KLEFVKVESC KFSNNLTLID TKLPEILAEM ILLYYSSKLN 

       250        260        270        280        290        300 
KIDDVTEHIS RLNPLNYNLS CNHNYYEYKV KHFLNDVALG MRPDDVWLGQ YDATGGYLVV 

       310        320        330        340        350 
KEDGELLCYH IYSKNSFEDY LYCNTKFDTP SSSRHDFGHI YQVNHDFFIK LNVQIRFL 

« Hide

References

[1]"Organization and sequence of the HpaII restriction-modification system and adjacent genes."
Kulakauskas S., Barsomian J.M., Lubys A., Roberts R.J., Wilson G.G.
Gene 142:9-15(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49669.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L17342 Genomic DNA. Translation: AAA20482.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1159. HpaII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019062. Restrct_endonuc_II_HpaII.
[Graphical view]
PfamPF09561. RE_HpaII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2H2_HAEPA
AccessionPrimary (citable) accession number: P36433
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries