P36433 (T2H2_HAEPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HpaII
Short name=R.HpaII
EC=3.1.21.4

Alternative name(s):
Endonuclease HpaII
Type II restriction enzyme HpaII

Gene names

Name:

hpaIIR

Organism

Haemophilus parainfluenzae

Taxonomic identifier

729 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCGG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Subunit structure	Homodimer.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Type-2 restriction enzyme HpaII		PRO_0000077325

Sequences

	Sequence		Length	Mass (Da)
	P36433 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: ECB312180C80303E	FASTA	358	40,926
	10 20 30 40 50 60 MTEFFSGNRG EWSEPYALFK LLADGQLYLG DSQLNKLGIV MPILSILRQE KNYESSYILH 70 80 90 100 110 120 NNSQNIIVTY NNEKFTVPIS GFQEKAVLLL SEIKNASGNR AFSIPSIDDF LKKLGFTHLS 130 140 150 160 170 180 ASSSSKSDIH IVVHDLRTGI TPTLGFSIKS QLGSPATLLN ASKATNFTFK IYNLKDKQIE 190 200 210 220 230 240 YINSLSGIKE KIKEIFSQDG KLEFVKVESC KFSNNLTLID TKLPEILAEM ILLYYSSKLN 250 260 270 280 290 300 KIDDVTEHIS RLNPLNYNLS CNHNYYEYKV KHFLNDVALG MRPDDVWLGQ YDATGGYLVV 310 320 330 340 350 KEDGELLCYH IYSKNSFEDY LYCNTKFDTP SSSRHDFGHI YQVNHDFFIK LNVQIRFL

References

[1]	"Organization and sequence of the HpaII restriction-modification system and adjacent genes." Kulakauskas S., Barsomian J.M., Lubys A., Roberts R.J., Wilson G.G. Gene 142:9-15(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49669.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L17342 Genomic DNA. Translation: AAA20482.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1159. HpaII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019062. Restrct_endonuc_II_HpaII. [Graphical view]
Pfam	PF09561. RE_HpaII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2H2_HAEPA

Accession

Primary (citable) accession number: P36433

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program