P36431 (SYP_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	CT_393

Organism

Chlamydia trachomatis

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 581	581	Proline--tRNA ligase HAMAP MF_01569		PRO_0000139327
Natural variations
	Natural variant	3	1	M → T in strain: E/UW-5/Cx.
	Natural variant	14	1	A → V in strain: E/UW-5/Cx.
	Natural variant	104	1	K → E in strain: E/UW-5/Cx.
	Natural variant	263	1	I → V in strain: E/UW-5/Cx.
	Natural variant	286	1	C → R in strain: E/UW-5/Cx.
	Natural variant	354	1	P → L in strain: E/UW-5/Cx.

Sequences

	Sequence		Length	Mass (Da)
	P36431 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 08815CF98623F259	FASTA	581	65,653
	10 20 30 40 50 60 MRMSLLFYRT SKNANKEASV LSYELLQKAG YLFKTSKGIY SYTPLFQRVI LKMTEIIREE 70 80 90 100 110 120 LNAIGGQEVC LPLLQPAELW EKTGRWKAFL SEKLLYVLKD RENKAMCLAP THEEVVSEFV 130 140 150 160 170 180 AQWLTGREQL PIHLYQIGTK FRDEIRPRFG LMRAKEFLME DSYTFSDSPE QMEEQYAKLR 190 200 210 220 230 240 LAYQRIFDRL NLKYVIVAAD GGKIGKGKSE EFHVLCSLGE DTICVSGSYG ANVEAAQAIP 250 260 270 280 290 300 PSYVYDSNLL PVEEVATPNI RTIEDLEVFF NTPKHKILKT LVVKTCQKDS EKFFAICIRG 310 320 330 340 350 360 DRQINLTKVA SFLQVDDCEL ASEEEILKHL HVEKGFIGPL YCPIPCYADE TTRPMTNFIC 370 380 390 400 410 420 ANNQKDVHCK HVNWGRDIPL PAFGDFLLAE AGDLCPQNGG APYEIFQGVE VAHIFNLGTR 430 440 450 460 470 480 YTESFSVGFQ DKNGDKQLCW MGTYGIGVGR TLAACIEQLA DNKGLVWPLA VAPFSITILY 490 500 510 520 530 540 NGGDTEGEAT ALQLYQSLNT EGFEPLLDDR NERLGFKLKD SDLLGIPYKL IIGKSFQSTG 550 560 570 580 LLEIESRSGE KCNVSPENLL DWCSKNLPCH TRKIPPLREQ N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Another putative heat-shock gene and aminoacyl-tRNA synthetase gene are located upstream from the grpE-like and dnaK-like genes in Chlamydia trachomatis." Schmiel D.H., Wyrick P.B. Gene 145:57-63(1994) [PubMed: 8045424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: E/UW-5/Cx.
[2]	"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L25105 Genomic DNA. Translation: AAA23161.1. AE001273 Genomic DNA. Translation: AAC67990.1.
PIR	G71520.
RefSeq	NP_219903.1. NC_000117.1.
3D structure databases
ProteinModelPortal	P36431.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	884722.
GenomeReviews	Gene locus CT_393 in contig AE001273_GR.
KEGG	ctr:CT393.
PATRIC	20380337. VBIChlTra43535_0423.
Phylogenomic databases
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_CHLTR

Accession

Primary (citable) accession number: P36431
Secondary accession number(s): O84398

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program