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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU30320-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BSU30320
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU30320. [Micado]

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Leucine--tRNA ligasePRO_0000151973Add
BLAST

Proteomic databases

PaxDbiP36430.

Interactioni

Protein-protein interaction databases

IntActiP36430. 1 interaction.
MINTiMINT-8365963.
STRINGi224308.Bsubs1_010100016511.

Structurei

3D structure databases

ProteinModelPortaliP36430.
SMRiP36430. Positions 2-803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
InParanoidiP36430.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.
PhylomeDBiP36430.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFQHKEIEK KWQTYWLENK TFATLDNNEK QKFYALDMFP YPSGAGLHVG
60 70 80 90 100
HPEGYTATDI LSRMKRMQGY DVLHPMGWDA FGLPAEQYAL DTGNDPAVFT
110 120 130 140 150
KQNIDNFRRQ IQALGFSYDW DREINTTDPE YYKWTQWIFL KLYEKGLAYV
160 170 180 190 200
DEVPVNWCPA LGTVLANEEV IDGKSERGGH PVERRPMKQW MLKITAYADR
210 220 230 240 250
LLEDLEELDW PESIKDMQRN WIGRSEGAHV HFAIDGHDDS FTVFTTRPDT
260 270 280 290 300
LFGATYTVLA PEHALVENIT TAEQKEAVEA YIKEIQSKSD LERTDLAKTK
310 320 330 340 350
TGVFTGAYAI NPVNGEKLPI WIADYVLASY GTGAVMAVPG HDERDFEFAK
360 370 380 390 400
TFGLPVKEVV KGGNVEEAAY TGDGEHVNSD FLNGLHKQEA IEKVIAWLEE
410 420 430 440 450
TKNGEKKVTY RLRDWLFSRQ RYWGEPIPVI HWEDGTSTAV PEEELPLILP
460 470 480 490 500
KTDEIKPSGT GESPLANIKE WVEVTDPETG KKGRRETNTM PQWAGSCWYF
510 520 530 540 550
LRYIDPHNPD QLASPEKLEK WLPVDMYIGG AEHAVLHLLY ARFWHKFLYD
560 570 580 590 600
IGVVPTKEPF QKLYNQGMIL GENNEKMSKS KGNVVNPDEI VASHGADTLR
610 620 630 640 650
LYEMFMGPLD ASIAWSESGL DGARRFLDRV WRLFIEDSGE LNGKIVEGAG
660 670 680 690 700
ETLERVYHET VMKVTDHYEG LRFNTGISQL MVFINEAYKA TELPKEYMEG
710 720 730 740 750
FVKLLSPVAP HLAEELWEKL GHSGTIAYEA WPVYDETKLV DDEVEIVVQL
760 770 780 790 800
NGKVKAKLQV PADATKEQLE QLAQADEKVK EQLEGKTIRK IIAVPGKLVN

IVAN
Length:804
Mass (Da):91,543
Last modified:July 15, 1998 - v3
Checksum:i306FD5A98FE5C47E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861P → L in AAA22571 (PubMed:1317842).Curated
Sequence conflicti195 – 1951T → N in AAA22571 (PubMed:1317842).Curated
Sequence conflicti247 – 28135RPDTL…AVEAY → DQIRCLALHTLSLPRNTHWW KTSQRQSKKKLLKLI (PubMed:1317842).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88581 Genomic DNA. Translation: AAA22571.1.
AF008220 Genomic DNA. Translation: AAC00259.1.
AL009126 Genomic DNA. Translation: CAB15010.1.
PIRiD69650.
RefSeqiNP_390910.1. NC_000964.3.
WP_003246072.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB15010; CAB15010; BSU30320.
GeneIDi938102.
KEGGibsu:BSU30320.
PATRICi18977966. VBIBacSub10457_3171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88581 Genomic DNA. Translation: AAA22571.1.
AF008220 Genomic DNA. Translation: AAC00259.1.
AL009126 Genomic DNA. Translation: CAB15010.1.
PIRiD69650.
RefSeqiNP_390910.1. NC_000964.3.
WP_003246072.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP36430.
SMRiP36430. Positions 2-803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP36430. 1 interaction.
MINTiMINT-8365963.
STRINGi224308.Bsubs1_010100016511.

Proteomic databases

PaxDbiP36430.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15010; CAB15010; BSU30320.
GeneIDi938102.
KEGGibsu:BSU30320.
PATRICi18977966. VBIBacSub10457_3171.

Organism-specific databases

GenoListiBSU30320. [Micado]

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
InParanoidiP36430.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.
PhylomeDBiP36430.

Enzyme and pathway databases

BioCyciBSUB:BSU30320-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis."
    Vander Horn P.B., Zahler S.A.
    J. Bacteriol. 174:3928-3935(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
    Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
    Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiSYL_BACSU
AccessioniPrimary (citable) accession number: P36430
Secondary accession number(s): O34465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 15, 1998
Last modified: July 22, 2015
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.