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P36422

- SYI_TETTH

UniProt

P36422 - SYI_TETTH

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Protein
Isoleucine--tRNA ligase
Gene
ILSA, CUPC
Organism
Tetrahymena thermophila
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei610 – 6101ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ILSA
Synonyms:CUPC
OrganismiTetrahymena thermophila
Taxonomic identifieri5911 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10811081Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098602Add
BLAST

Proteomic databases

PRIDEiP36422.

Interactioni

Protein-protein interaction databases

STRINGi5911.P36422.

Structurei

3D structure databases

ProteinModelPortaliP36422.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionUniRule annotation
Add
BLAST
Motifi607 – 6115"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36422-1 [UniParc]FASTAAdd to Basket

« Hide

MASKTTFKDL QEIPDFPKEE ENILKFWDEI NAFKQQLEKT KDCPPFTFYD     50
GPPFATGLPH YGNLLAGTIK DVVCRYASQN GKYVERRFGW DCHGLPVEYE 100
IDKKLGITNR QEVLKMGVDK YNAECRSIVM RYAQEWRSIV NRFGRWVDFD 150
NDYKTLDLKF MESVWWVFKQ MFDKGLVYRG CKVMPYSNGC ATVLSNFETQ 200
QNYKEVDDPS LFIAFKTAED PKTKFIAWTT TPWTLPSNLA LVINKDFDYV 250
KVLDAKTQEH YILAECRLPE LYKKDKDGYK ILEKFKGSEL VGREYEPLFP 300
YFLSRKQDGC FRILAGDFVT ADAGTGIVHC APGFGDDDYK VSVANNIIKP 350
DDPPVPVDEN GHFTNVVSDF AGVYIKEADK LIRKNLKERG LLLVDSSFKH 400
NYPFCWRSDT PLIYKAVHCW FIKVTALKDD LLANNKKAYW VPKFAQEGRF 450
NNWLQNVSDW CFSRSRFWGN PIPIWVSEDF EEVVCIGSVE ELKKLTGATE 500
ITDLHKDFID HLTIPSQKGK GVLRRIDEVF DCWFESGSMP YGQQHYPFSM 550
NEEEFSKRFP ADFIGEGIDQ TRGWFYTLNV ISTALRNSNP YKNLIVNGIV 600
LAADGKKMSK SKKNYDDPLL IASKYSVDAI RLYMINSPLV RAEEMSFKSE 650
GVFAVKKDIF LPWYNAYKFL IQSITRWELA TGKDYMFNEQ LSVDTTKLTN 700
PTDRWIIISC QNLINYVRIE MEKYHLYNVV PRLIHFLENL TNWYIRLNRN 750
RLKGDYGLEE QETSLNVLFN VILNSTILMS PLVPFITESF YQNLRKVIPK 800
GSSYLEESIH FLRIPTPKQE LLDEKIERNF ERMQNIINFA RTLREKRKVS 850
LKQPIMSLTV INQDQEFHDS LKDYIQYIED EINTPSILHE INTANYVDLK 900
AIPNHKLLGQ KLGKEYNKDL KAAAGNLSAK DIETLKTTGS IDLVGKKLLL 950
EDFTITQNYK KEYSSGDLEL GGEGEVILLL NLAQDEKLKS KGLVREFTSN 1000
VQKTKKKTGL KVDDNIVIYY DVTKAPKLNA AIQSDLEAVQ KVLKKPLVPL 1050
SEKNAALKIV ENSLLTFQVD EESVTIEIAY A 1081
Length:1,081
Mass (Da):124,851
Last modified:June 1, 1994 - v1
Checksum:iD4D72616AFB8C795
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30942 Genomic DNA. Translation: AAA30122.1.
PIRiA42399.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30942 Genomic DNA. Translation: AAA30122.1 .
PIRi A42399.

3D structure databases

ProteinModelPortali P36422.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5911.P36422.

Proteomic databases

PRIDEi P36422.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0060.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isoleucyl-tRNA synthetase from the ciliated protozoan Tetrahymena thermophila. DNA sequence, gene regulation, and leucine zipper motifs."
    Csank C., Martindale D.W.
    J. Biol. Chem. 267:4592-4599(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiSYI_TETTH
AccessioniPrimary (citable) accession number: P36422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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