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P36422

- SYI_TETTH

UniProt

P36422 - SYI_TETTH

Protein

Isoleucine--tRNA ligase

Gene

ILSA

Organism
Tetrahymena thermophila
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei610 – 6101ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:ILSA
    Synonyms:CUPC
    OrganismiTetrahymena thermophila
    Taxonomic identifieri5911 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10811081Isoleucine--tRNA ligasePRO_0000098602Add
    BLAST

    Proteomic databases

    PRIDEiP36422.

    Interactioni

    Protein-protein interaction databases

    STRINGi5911.P36422.

    Structurei

    3D structure databases

    ProteinModelPortaliP36422.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi53 – 6311"HIGH" regionAdd
    BLAST
    Motifi607 – 6115"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36422-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASKTTFKDL QEIPDFPKEE ENILKFWDEI NAFKQQLEKT KDCPPFTFYD     50
    GPPFATGLPH YGNLLAGTIK DVVCRYASQN GKYVERRFGW DCHGLPVEYE 100
    IDKKLGITNR QEVLKMGVDK YNAECRSIVM RYAQEWRSIV NRFGRWVDFD 150
    NDYKTLDLKF MESVWWVFKQ MFDKGLVYRG CKVMPYSNGC ATVLSNFETQ 200
    QNYKEVDDPS LFIAFKTAED PKTKFIAWTT TPWTLPSNLA LVINKDFDYV 250
    KVLDAKTQEH YILAECRLPE LYKKDKDGYK ILEKFKGSEL VGREYEPLFP 300
    YFLSRKQDGC FRILAGDFVT ADAGTGIVHC APGFGDDDYK VSVANNIIKP 350
    DDPPVPVDEN GHFTNVVSDF AGVYIKEADK LIRKNLKERG LLLVDSSFKH 400
    NYPFCWRSDT PLIYKAVHCW FIKVTALKDD LLANNKKAYW VPKFAQEGRF 450
    NNWLQNVSDW CFSRSRFWGN PIPIWVSEDF EEVVCIGSVE ELKKLTGATE 500
    ITDLHKDFID HLTIPSQKGK GVLRRIDEVF DCWFESGSMP YGQQHYPFSM 550
    NEEEFSKRFP ADFIGEGIDQ TRGWFYTLNV ISTALRNSNP YKNLIVNGIV 600
    LAADGKKMSK SKKNYDDPLL IASKYSVDAI RLYMINSPLV RAEEMSFKSE 650
    GVFAVKKDIF LPWYNAYKFL IQSITRWELA TGKDYMFNEQ LSVDTTKLTN 700
    PTDRWIIISC QNLINYVRIE MEKYHLYNVV PRLIHFLENL TNWYIRLNRN 750
    RLKGDYGLEE QETSLNVLFN VILNSTILMS PLVPFITESF YQNLRKVIPK 800
    GSSYLEESIH FLRIPTPKQE LLDEKIERNF ERMQNIINFA RTLREKRKVS 850
    LKQPIMSLTV INQDQEFHDS LKDYIQYIED EINTPSILHE INTANYVDLK 900
    AIPNHKLLGQ KLGKEYNKDL KAAAGNLSAK DIETLKTTGS IDLVGKKLLL 950
    EDFTITQNYK KEYSSGDLEL GGEGEVILLL NLAQDEKLKS KGLVREFTSN 1000
    VQKTKKKTGL KVDDNIVIYY DVTKAPKLNA AIQSDLEAVQ KVLKKPLVPL 1050
    SEKNAALKIV ENSLLTFQVD EESVTIEIAY A 1081
    Length:1,081
    Mass (Da):124,851
    Last modified:June 1, 1994 - v1
    Checksum:iD4D72616AFB8C795
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30942 Genomic DNA. Translation: AAA30122.1.
    PIRiA42399.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30942 Genomic DNA. Translation: AAA30122.1 .
    PIRi A42399.

    3D structure databases

    ProteinModelPortali P36422.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5911.P36422.

    Proteomic databases

    PRIDEi P36422.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0060.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isoleucyl-tRNA synthetase from the ciliated protozoan Tetrahymena thermophila. DNA sequence, gene regulation, and leucine zipper motifs."
      Csank C., Martindale D.W.
      J. Biol. Chem. 267:4592-4599(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiSYI_TETTH
    AccessioniPrimary (citable) accession number: P36422
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3