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P36422

- SYI_TETTH

UniProt

P36422 - SYI_TETTH

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Protein

Isoleucine--tRNA ligase

Gene

ILSA

Organism
Tetrahymena thermophila
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei610 – 6101ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ILSA
Synonyms:CUPC
OrganismiTetrahymena thermophila
Taxonomic identifieri5911 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10811081Isoleucine--tRNA ligasePRO_0000098602Add
BLAST

Proteomic databases

PRIDEiP36422.

Interactioni

Protein-protein interaction databases

STRINGi5911.P36422.

Structurei

3D structure databases

ProteinModelPortaliP36422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi607 – 6115"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36422-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASKTTFKDL QEIPDFPKEE ENILKFWDEI NAFKQQLEKT KDCPPFTFYD
60 70 80 90 100
GPPFATGLPH YGNLLAGTIK DVVCRYASQN GKYVERRFGW DCHGLPVEYE
110 120 130 140 150
IDKKLGITNR QEVLKMGVDK YNAECRSIVM RYAQEWRSIV NRFGRWVDFD
160 170 180 190 200
NDYKTLDLKF MESVWWVFKQ MFDKGLVYRG CKVMPYSNGC ATVLSNFETQ
210 220 230 240 250
QNYKEVDDPS LFIAFKTAED PKTKFIAWTT TPWTLPSNLA LVINKDFDYV
260 270 280 290 300
KVLDAKTQEH YILAECRLPE LYKKDKDGYK ILEKFKGSEL VGREYEPLFP
310 320 330 340 350
YFLSRKQDGC FRILAGDFVT ADAGTGIVHC APGFGDDDYK VSVANNIIKP
360 370 380 390 400
DDPPVPVDEN GHFTNVVSDF AGVYIKEADK LIRKNLKERG LLLVDSSFKH
410 420 430 440 450
NYPFCWRSDT PLIYKAVHCW FIKVTALKDD LLANNKKAYW VPKFAQEGRF
460 470 480 490 500
NNWLQNVSDW CFSRSRFWGN PIPIWVSEDF EEVVCIGSVE ELKKLTGATE
510 520 530 540 550
ITDLHKDFID HLTIPSQKGK GVLRRIDEVF DCWFESGSMP YGQQHYPFSM
560 570 580 590 600
NEEEFSKRFP ADFIGEGIDQ TRGWFYTLNV ISTALRNSNP YKNLIVNGIV
610 620 630 640 650
LAADGKKMSK SKKNYDDPLL IASKYSVDAI RLYMINSPLV RAEEMSFKSE
660 670 680 690 700
GVFAVKKDIF LPWYNAYKFL IQSITRWELA TGKDYMFNEQ LSVDTTKLTN
710 720 730 740 750
PTDRWIIISC QNLINYVRIE MEKYHLYNVV PRLIHFLENL TNWYIRLNRN
760 770 780 790 800
RLKGDYGLEE QETSLNVLFN VILNSTILMS PLVPFITESF YQNLRKVIPK
810 820 830 840 850
GSSYLEESIH FLRIPTPKQE LLDEKIERNF ERMQNIINFA RTLREKRKVS
860 870 880 890 900
LKQPIMSLTV INQDQEFHDS LKDYIQYIED EINTPSILHE INTANYVDLK
910 920 930 940 950
AIPNHKLLGQ KLGKEYNKDL KAAAGNLSAK DIETLKTTGS IDLVGKKLLL
960 970 980 990 1000
EDFTITQNYK KEYSSGDLEL GGEGEVILLL NLAQDEKLKS KGLVREFTSN
1010 1020 1030 1040 1050
VQKTKKKTGL KVDDNIVIYY DVTKAPKLNA AIQSDLEAVQ KVLKKPLVPL
1060 1070 1080
SEKNAALKIV ENSLLTFQVD EESVTIEIAY A
Length:1,081
Mass (Da):124,851
Last modified:June 1, 1994 - v1
Checksum:iD4D72616AFB8C795
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30942 Genomic DNA. Translation: AAA30122.1.
PIRiA42399.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30942 Genomic DNA. Translation: AAA30122.1 .
PIRi A42399.

3D structure databases

ProteinModelPortali P36422.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5911.P36422.

Proteomic databases

PRIDEi P36422.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0060.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isoleucyl-tRNA synthetase from the ciliated protozoan Tetrahymena thermophila. DNA sequence, gene regulation, and leucine zipper motifs."
    Csank C., Martindale D.W.
    J. Biol. Chem. 267:4592-4599(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiSYI_TETTH
AccessioniPrimary (citable) accession number: P36422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3