P36422 (SYI_TETTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ILSA
Synonyms:	CUPC

Organism

Tetrahymena thermophila

Taxonomic identifier

5911 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Hymenostomatida › Tetrahymenina › Tetrahymenidae › Tetrahymena

Protein attributes

Sequence length	1081 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1081	1081	Isoleucine--tRNA ligase		PRO_0000098602
Regions
	Motif	53 – 63	11	"HIGH" region
	Motif	607 – 611	5	"KMSKS" region
Sites
	Binding site	610	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36422 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: D4D72616AFB8C795	FASTA	1,081	124,851
	10 20 30 40 50 60 MASKTTFKDL QEIPDFPKEE ENILKFWDEI NAFKQQLEKT KDCPPFTFYD GPPFATGLPH 70 80 90 100 110 120 YGNLLAGTIK DVVCRYASQN GKYVERRFGW DCHGLPVEYE IDKKLGITNR QEVLKMGVDK 130 140 150 160 170 180 YNAECRSIVM RYAQEWRSIV NRFGRWVDFD NDYKTLDLKF MESVWWVFKQ MFDKGLVYRG 190 200 210 220 230 240 CKVMPYSNGC ATVLSNFETQ QNYKEVDDPS LFIAFKTAED PKTKFIAWTT TPWTLPSNLA 250 260 270 280 290 300 LVINKDFDYV KVLDAKTQEH YILAECRLPE LYKKDKDGYK ILEKFKGSEL VGREYEPLFP 310 320 330 340 350 360 YFLSRKQDGC FRILAGDFVT ADAGTGIVHC APGFGDDDYK VSVANNIIKP DDPPVPVDEN 370 380 390 400 410 420 GHFTNVVSDF AGVYIKEADK LIRKNLKERG LLLVDSSFKH NYPFCWRSDT PLIYKAVHCW 430 440 450 460 470 480 FIKVTALKDD LLANNKKAYW VPKFAQEGRF NNWLQNVSDW CFSRSRFWGN PIPIWVSEDF 490 500 510 520 530 540 EEVVCIGSVE ELKKLTGATE ITDLHKDFID HLTIPSQKGK GVLRRIDEVF DCWFESGSMP 550 560 570 580 590 600 YGQQHYPFSM NEEEFSKRFP ADFIGEGIDQ TRGWFYTLNV ISTALRNSNP YKNLIVNGIV 610 620 630 640 650 660 LAADGKKMSK SKKNYDDPLL IASKYSVDAI RLYMINSPLV RAEEMSFKSE GVFAVKKDIF 670 680 690 700 710 720 LPWYNAYKFL IQSITRWELA TGKDYMFNEQ LSVDTTKLTN PTDRWIIISC QNLINYVRIE 730 740 750 760 770 780 MEKYHLYNVV PRLIHFLENL TNWYIRLNRN RLKGDYGLEE QETSLNVLFN VILNSTILMS 790 800 810 820 830 840 PLVPFITESF YQNLRKVIPK GSSYLEESIH FLRIPTPKQE LLDEKIERNF ERMQNIINFA 850 860 870 880 890 900 RTLREKRKVS LKQPIMSLTV INQDQEFHDS LKDYIQYIED EINTPSILHE INTANYVDLK 910 920 930 940 950 960 AIPNHKLLGQ KLGKEYNKDL KAAAGNLSAK DIETLKTTGS IDLVGKKLLL EDFTITQNYK 970 980 990 1000 1010 1020 KEYSSGDLEL GGEGEVILLL NLAQDEKLKS KGLVREFTSN VQKTKKKTGL KVDDNIVIYY 1030 1040 1050 1060 1070 1080 DVTKAPKLNA AIQSDLEAVQ KVLKKPLVPL SEKNAALKIV ENSLLTFQVD EESVTIEIAY A

References

[1]	"Isoleucyl-tRNA synthetase from the ciliated protozoan Tetrahymena thermophila. DNA sequence, gene regulation, and leucine zipper motifs." Csank C., Martindale D.W. J. Biol. Chem. 267:4592-4599(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M30942 Genomic DNA. Translation: AAA30122.1.
PIR	A42399.
3D structure databases
ProteinModelPortal	P36422.
ModBase	Search...
Protein-protein interaction databases
STRING	5911.P36422.
Proteomic databases
PRIDE	P36422.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG0060.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_TETTH

Accession

Primary (citable) accession number: P36422

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)