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P36422 (SYI_TETTH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ILSA
Synonyms:CUPC
OrganismTetrahymena thermophila
Taxonomic identifier5911 [NCBI]
Taxonomic lineageEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Protein attributes

Sequence length1081 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10811081Isoleucine--tRNA ligase HAMAP-Rule MF_02003
PRO_0000098602

Regions

Motif53 – 6311"HIGH" region HAMAP-Rule MF_02003
Motif607 – 6115"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site6101ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P36422 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: D4D72616AFB8C795

FASTA1,081124,851
        10         20         30         40         50         60 
MASKTTFKDL QEIPDFPKEE ENILKFWDEI NAFKQQLEKT KDCPPFTFYD GPPFATGLPH 

        70         80         90        100        110        120 
YGNLLAGTIK DVVCRYASQN GKYVERRFGW DCHGLPVEYE IDKKLGITNR QEVLKMGVDK 

       130        140        150        160        170        180 
YNAECRSIVM RYAQEWRSIV NRFGRWVDFD NDYKTLDLKF MESVWWVFKQ MFDKGLVYRG 

       190        200        210        220        230        240 
CKVMPYSNGC ATVLSNFETQ QNYKEVDDPS LFIAFKTAED PKTKFIAWTT TPWTLPSNLA 

       250        260        270        280        290        300 
LVINKDFDYV KVLDAKTQEH YILAECRLPE LYKKDKDGYK ILEKFKGSEL VGREYEPLFP 

       310        320        330        340        350        360 
YFLSRKQDGC FRILAGDFVT ADAGTGIVHC APGFGDDDYK VSVANNIIKP DDPPVPVDEN 

       370        380        390        400        410        420 
GHFTNVVSDF AGVYIKEADK LIRKNLKERG LLLVDSSFKH NYPFCWRSDT PLIYKAVHCW 

       430        440        450        460        470        480 
FIKVTALKDD LLANNKKAYW VPKFAQEGRF NNWLQNVSDW CFSRSRFWGN PIPIWVSEDF 

       490        500        510        520        530        540 
EEVVCIGSVE ELKKLTGATE ITDLHKDFID HLTIPSQKGK GVLRRIDEVF DCWFESGSMP 

       550        560        570        580        590        600 
YGQQHYPFSM NEEEFSKRFP ADFIGEGIDQ TRGWFYTLNV ISTALRNSNP YKNLIVNGIV 

       610        620        630        640        650        660 
LAADGKKMSK SKKNYDDPLL IASKYSVDAI RLYMINSPLV RAEEMSFKSE GVFAVKKDIF 

       670        680        690        700        710        720 
LPWYNAYKFL IQSITRWELA TGKDYMFNEQ LSVDTTKLTN PTDRWIIISC QNLINYVRIE 

       730        740        750        760        770        780 
MEKYHLYNVV PRLIHFLENL TNWYIRLNRN RLKGDYGLEE QETSLNVLFN VILNSTILMS 

       790        800        810        820        830        840 
PLVPFITESF YQNLRKVIPK GSSYLEESIH FLRIPTPKQE LLDEKIERNF ERMQNIINFA 

       850        860        870        880        890        900 
RTLREKRKVS LKQPIMSLTV INQDQEFHDS LKDYIQYIED EINTPSILHE INTANYVDLK 

       910        920        930        940        950        960 
AIPNHKLLGQ KLGKEYNKDL KAAAGNLSAK DIETLKTTGS IDLVGKKLLL EDFTITQNYK 

       970        980        990       1000       1010       1020 
KEYSSGDLEL GGEGEVILLL NLAQDEKLKS KGLVREFTSN VQKTKKKTGL KVDDNIVIYY 

      1030       1040       1050       1060       1070       1080 
DVTKAPKLNA AIQSDLEAVQ KVLKKPLVPL SEKNAALKIV ENSLLTFQVD EESVTIEIAY 


A 

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References

[1]"Isoleucyl-tRNA synthetase from the ciliated protozoan Tetrahymena thermophila. DNA sequence, gene regulation, and leucine zipper motifs."
Csank C., Martindale D.W.
J. Biol. Chem. 267:4592-4599(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M30942 Genomic DNA. Translation: AAA30122.1.
PIRA42399.

3D structure databases

ProteinModelPortalP36422.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5911.P36422.

Proteomic databases

PRIDEP36422.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0060.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_TETTH
AccessionPrimary (citable) accession number: P36422
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries