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Protein

Tyrosine--tRNA ligase, cytoplasmic

Gene

TYS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of L-tyrosine to tRNA(Tyr) in a two-step reaction: L-tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). The specificity determinants on tRNA(Tyr) are the base pair C1-G72, the discriminator residue A73, and the three anticodon bases G34, U35 and A36. Also involved in nuclear tRNA export. Also attaches D-Tyr to tRNA(Tyr), this reaction is about 150-fold less efficient than attachment of L-Tyr (PubMed:10766779).3 Publications

Miscellaneous

Present with 2710 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).

Enzyme regulationi

Inhibited by N-ethylmaleimide and p-chloromercuribenzoate.1 Publication

Kineticsi

The catalytic activity is reduced by substitutions of residues forming the specificity determinant on the target tRNA(Tyr).
  1. KM=54 nM for tRNA(Tyr)1 Publication
  1. Vmax=280 nmol/min/mg enzyme for tRNA(Tyr)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43TyrosineBy similarity1
Binding sitei170TyrosineBy similarity1
Binding sitei174TyrosineBy similarity1
Binding sitei177TyrosineBy similarity1
Binding sitei192TyrosineBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • tyrosine-tRNA ligase activity Source: SGD

GO - Biological processi

  • tyrosyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30875-MONOMER
BRENDAi6.1.1.1 984

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligase, cytoplasmic (EC:6.1.1.1)
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name:
TyrRS
Gene namesi
Name:TYS1
Synonyms:MGM104
Ordered Locus Names:YGR185C
ORF Names:G7522
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR185C
SGDiS000003417 TYS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43Y → G: Decreases catalytic activity for L-tyrosine 400-fold, but allows the utilization of 3-iodo-L-tyrosine and other 3-modified tyrosines as substrates. 1 Publication1
Mutagenesisi364 – 368KKAKK → EEAEE: Abolishes nuclear localization. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000556762 – 394Tyrosine--tRNA ligase, cytoplasmicAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei235PhosphoserineCombined sources1
Modified residuei359PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP36421
PaxDbiP36421
PRIDEiP36421

PTM databases

iPTMnetiP36421

Interactioni

Subunit structurei

Homodimer. Interacts with KNR4/SMI1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SMI1P325663EBI-18843,EBI-17452

Protein-protein interaction databases

BioGridi33437, 218 interactors
DIPiDIP-5548N
IntActiP36421, 17 interactors
MINTiP36421
STRINGi4932.YGR185C

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 17Combined sources9
Beta strandi21 – 24Combined sources4
Helixi26 – 34Combined sources9
Beta strandi41 – 46Combined sources6
Helixi54 – 56Combined sources3
Helixi57 – 68Combined sources12
Beta strandi72 – 77Combined sources6
Helixi79 – 84Combined sources6
Turni85 – 87Combined sources3
Helixi93 – 111Combined sources19
Beta strandi120 – 123Combined sources4
Helixi125 – 128Combined sources4
Helixi131 – 141Combined sources11
Helixi146 – 152Combined sources7
Turni153 – 156Combined sources4
Helixi166 – 180Combined sources15
Beta strandi184 – 189Combined sources6
Helixi190 – 192Combined sources3
Helixi193 – 202Combined sources10
Helixi203 – 206Combined sources4
Beta strandi212 – 216Combined sources5
Helixi243 – 252Combined sources10
Helixi264 – 271Combined sources8
Helixi273 – 278Combined sources6
Beta strandi280 – 282Combined sources3
Beta strandi288 – 290Combined sources3
Helixi294 – 296Combined sources3
Beta strandi300 – 304Combined sources5
Helixi305 – 313Combined sources9
Helixi319 – 343Combined sources25
Helixi345 – 354Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DLCX-ray2.40X1-394[»]
ProteinModelPortaliP36421
SMRiP36421
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36421

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 56"HIGH" region9
Motifi227 – 231"KMSKS" region5
Motifi360 – 378Nuclear localization signalAdd BLAST19

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00790000123100
HOGENOMiHOG000228237
InParanoidiP36421
KOiK01866
OMAiYIGFEIS
OrthoDBiEOG092C2KRE

Family and domain databases

CDDicd00805 TyrRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002307 Tyr-tRNA-ligase
IPR023617 Tyr-tRNA-ligase_arc/euk-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PIRSFiPIRSF006588 TyrRS_arch_euk, 1 hit
PRINTSiPR01040 TRNASYNTHTYR
TIGRFAMsiTIGR00234 tyrS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36421-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAATVDPN EAFGLITKNL QEVLNPQIIK DVLEVQKRHL KLYWGTAPTG
60 70 80 90 100
RPHCGYFVPM TKLADFLKAG CEVTVLLADL HAFLDNMKAP LEVVNYRAKY
110 120 130 140 150
YELTIKAILR SINVPIEKLK FVVGSSYQLT PDYTMDIFRL SNIVSQNDAK
160 170 180 190 200
RAGADVVKQV ANPLLSGLIY PLMQALDEQF LDVDCQFGGV DQRKIFVLAE
210 220 230 240 250
ENLPSLGYKK RAHLMNPMVP GLAQGGKMSA SDPNSKIDLL EEPKQVKKKI
260 270 280 290 300
NSAFCSPGNV EENGLLSFVQ YVIAPIQELK FGTNHFEFFI DRPEKFGGPI
310 320 330 340 350
TYKSFEEMKL AFKEEKLSPP DLKIGVADAI NELLEPIRQE FANNKEFQEA
360 370 380 390
SEKGYPVATP QKSKKAKKPK NKGTKYPGAT KTNEIATKLE ETKL
Length:394
Mass (Da):44,020
Last modified:January 23, 2007 - v3
Checksum:i57E8DB9BE6D054B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12221 mRNA Translation: AAB59329.1
X71998 Genomic DNA No translation available.
X99074 Genomic DNA Translation: CAA67529.1
Z72970 Genomic DNA Translation: CAA97211.1
U13015 Genomic DNA Translation: AAA61641.1
BK006941 Genomic DNA Translation: DAA08279.1
PIRiA45999
RefSeqiNP_011701.3, NM_001181314.3

Genome annotation databases

EnsemblFungiiYGR185C; YGR185C; YGR185C
GeneIDi853097
KEGGisce:YGR185C

Similar proteinsi

Entry informationi

Entry nameiSYYC_YEAST
AccessioniPrimary (citable) accession number: P36421
Secondary accession number(s): D6VUW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health