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Protein

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Gene

pdhC

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

Acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine.

Cofactori

(R)-lipoateBy similarityNote: Binds 2 lipoyl cofactors covalently.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processGlycolysis

Enzyme and pathway databases

ReactomeiR-DDI-389661 Glyoxylate metabolism and glycine degradation
R-DDI-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (EC:2.3.1.12)
Alternative name(s):
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2
Short name:
PDC-E2
Short name:
PDCE2
Gene namesi
Name:pdhC
Synonyms:dlaA
ORF Names:DDB_G0277847
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0277847 pdhC

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000020480? – 635Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Transit peptidei1 – ?Mitochondrion

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei124N6-lipoyllysinePROSITE-ProRule annotationBy similarity1
Modified residuei247N6-lipoyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

PaxDbiP36413
PRIDEiP36413

Interactioni

Subunit structurei

20 to 30 alpha2-beta2 tetramers of E1 + 6 homodimers of E3 + 60 copies of E2.

Protein-protein interaction databases

STRINGi44689.DDB0215387

Structurei

3D structure databases

ProteinModelPortaliP36413
SMRiP36413
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 160Lipoyl-binding 1PROSITE-ProRule annotationAdd BLAST78
Domaini206 – 283Lipoyl-binding 2PROSITE-ProRule annotationAdd BLAST78
Domaini342 – 379Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST38

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni403 – 635CatalyticBy similarityAdd BLAST233

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi301 – 314Ser/Thr-rich (hinge)Add BLAST14

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated

Keywords - Domaini

Lipoyl, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0557 Eukaryota
COG0508 LUCA
InParanoidiP36413
KOiK00627
OMAiTMEFESF
PhylomeDBiP36413

Family and domain databases

Gene3Di3.30.559.10, 1 hit
4.10.320.10, 1 hit
InterProiView protein in InterPro
IPR001078 2-oxoacid_DH_actylTfrase
IPR000089 Biotin_lipoyl
IPR023213 CAT-like_dom_sf
IPR036625 E3-bd_dom_sf
IPR006257 LAT1
IPR004167 PSBD
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF00198 2-oxoacid_dh, 1 hit
PF00364 Biotin_lipoyl, 2 hits
PF02817 E3_binding, 1 hit
SUPFAMiSSF47005 SSF47005, 1 hit
SSF51230 SSF51230, 2 hits
TIGRFAMsiTIGR01349 PDHac_trf_mito, 1 hit
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 2 hits
PS00189 LIPOYL, 2 hits
PS51826 PSBD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36413-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRAINQNSA KVVKSLKQQL VVLEATNVVA YTGTKSFTTT KTFNNTQTKP
60 70 80 90 100
KIFTSSNVLS FSSPSSSNVF SEILNKRSYS SKGKEITMPA LSPSMTEGNI
110 120 130 140 150
VQWKKKEGDQ IKAGDVIAEV ETDKATMDFQ YEDGNGYLAK ILIPEGTKGI
160 170 180 190 200
EINKPIAIIV SKKEDIESAV KNYKPSSQAS STPVQEEAPK PKQEAPKKST
210 220 230 240 250
KTYPAHKVVG MPALSPSMET GGIASWTKKE GDQIKAGDAI AEVETDKATM
260 270 280 290 300
DFQYEDGNGY LAKILVPGGT SGIQINQPVC IIVKNKEDCD KFADYSVEEQ
310 320 330 340 350
SSSSSSSSQE STPSSSSSSS QESTPSQSSS QQTTRKSGER IFATPAARFE
360 370 380 390 400
ASSKGYDLSA INGTGPNNRI LKADVLEFVP QKQEVAQQQQ QQTTTTTKKP
410 420 430 440 450
TTPTSSGEFT DIPHSNIRKV TAARLTESKQ TIPHYYLTME CRVDKLLKLR
460 470 480 490 500
SELNAMNTVK ISVNDFIVKA SAAALRDNPV VNSTWTDQFI RRYHNIDINV
510 520 530 540 550
AVNTPQGLFT PIVRGVDMKG LNSISTSVKQ LAEKAQNGKL HPSEFESGTF
560 570 580 590 600
TISNLGMLGI KQFAAVINPP QAAILAVGTT ETRVVLSNKP DSPYETATIL
610 620 630
SVTLSCDHRV IDGAVGAEWL KSFKDYVENP IKLIL
Length:635
Mass (Da):68,986
Last modified:December 4, 2007 - v2
Checksum:iDC1B4924A680B2AB
GO

Sequence cautioni

The sequence AAA16511 differs from that shown. Reason: Frameshift at positions 577 and 585.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97E → V in AAA16511 (Ref. 2) Curated1
Sequence conflicti118A → R in AAA16511 (Ref. 2) Curated1
Sequence conflicti129 – 130FQ → S in AAA16511 (Ref. 2) Curated2
Sequence conflicti472 – 473AA → LP in AAA16511 (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA Translation: EAL68096.1
U06634 mRNA Translation: AAA16511.1 Frameshift.
RefSeqiXP_642438.1, XM_637346.1

Genome annotation databases

EnsemblProtistsiEAL68096; EAL68096; DDB_G0277847
GeneIDi8621644
KEGGiddi:DDB_G0277847

Similar proteinsi

Entry informationi

Entry nameiODP2_DICDI
AccessioniPrimary (citable) accession number: P36413
Secondary accession number(s): Q54XW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 4, 2007
Last modified: May 23, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health