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Protein

E3 ubiquitin-protein ligase TRIM23

Gene

Trim23

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri31 – 76RING-type; degeneratePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri122 – 168B box-type; degeneratePROSITE-ProRule annotationAdd BLAST47
Nucleotide bindingi411 – 418GTPBy similarity8
Nucleotide bindingi454 – 458GTPBy similarity5
Nucleotide bindingi513 – 516GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • protein ADP-ribosylation Source: RGD
  • protein ubiquitination Source: HGNC

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandGTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM23 (EC:2.3.2.27)
Alternative name(s):
ADP-ribosylation factor domain-containing protein 1
GTP-binding protein ARD-1
RING-type E3 ubiquitin transferase TRIM23Curated
Tripartite motif-containing protein 23
Gene namesi
Name:Trim23
Synonyms:Ard-1, Ard1, Arfd1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621587 Trim23

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002074851 – 573E3 ubiquitin-protein ligase TRIM23Add BLAST573

Proteomic databases

PaxDbiP36407
PRIDEiP36407

Interactioni

Subunit structurei

Homodimer. Interacts with PSCD1. Interacts with UBE2D2. Interacts with TBK1 (via N-terminal kinase domain) and p62/SQSTM1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2494951 interactor.
STRINGi10116.ENSRNOP00000016647

Structurei

3D structure databases

ProteinModelPortaliP36407
SMRiP36407
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni390 – 573ARF-likeAdd BLAST184

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili351 – 378Sequence analysisAdd BLAST28

Domaini

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the small GTPase superfamily. Arf family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri31 – 76RING-type; degeneratePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri122 – 168B box-type; degeneratePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
KOG4185 Eukaryota
ENOG410ZGPK LUCA
HOVERGENiHBG000551
InParanoidiP36407
KOiK07963
PhylomeDBiP36407

Family and domain databases

CDDicd00021 BBOX, 1 hit
Gene3Di3.30.40.101 hit
InterProiView protein in InterPro
IPR003649 Bbox_C
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
IPR027370 Znf-RING_LisH
IPR000315 Znf_B-box
IPR013087 Znf_C2H2_type
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PF00643 zf-B_box, 1 hit
PF13445 zf-RING_UBOX, 1 hit
PRINTSiPR00328 SAR1GTPBP
SMARTiView protein in SMART
SM00502 BBC, 1 hit
SM00336 BBOX, 2 hits
SM00184 RING, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P36407-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALAVNKPG AGVDSGRQGS RGTAVVKVLE CGVCEDVFSL QGDKVPRLLL
60 70 80 90 100
CGHTVCHDCL TRLPLHGRAI RCPFDRQVTD LGDSGVWGLK KNFALLELLE
110 120 130 140 150
RLQNGHIGQY GAAEEALGIS GESIIRCDED EAHVASVYCT VCATHLCSEC
160 170 180 190 200
SQVTHSTKTL AKHRRVPLAD KPHEKTMCCQ HQVHAIEFVC LEEACQTSPL
210 220 230 240 250
MCCVCKEYGK HQGHKHSVLE PEANQIRASI LDMAHCIRTF TEEISDYSRK
260 270 280 290 300
LVGIVQHIEG GEQIVEDGIG MAHTEHVPGT AENARSCVRA YFSDLHETLC
310 320 330 340 350
RQEEMALSVV DAHVREKLIW LRQQQEDMTI LLSQVSTACL HCKTLQQDDC
360 370 380 390 400
RVVLAKQEIT RLLETLQKQQ QQFTEVADHI QLDASIPVTF TKDNRVYHGP
410 420 430 440 450
KMEIRVVTLG LDGAGKTTIL FKLKQDEFMQ PIPTIGFNVE TVEYKNLKFT
460 470 480 490 500
IWDVGGKHKL RPLWKHYYLN TQAVVFVVDS SHRDRISEAH SELAKLLTEK
510 520 530 540 550
ELRDALLLIF ANKQDVAGAL SVEEITELLS LHKLCCGRSW YIQGCDARSG
560 570
MGLYEGLDWL SRQLVAAGVL DVA
Length:573
Mass (Da):63,892
Last modified:August 10, 2010 - v2
Checksum:i62263E1DE6C2C909
GO
Isoform 2 (identifier: P36407-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     216-276: Missing.

Show »
Length:512
Mass (Da):57,098
Checksum:i79B2CFF27AAF5814
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61T → S in AAA41301 (PubMed:15057822).Curated1
Sequence conflicti342C → CE in AAA41301 (PubMed:15057822).Curated1
Sequence conflicti398H → Y in AAA41301 (PubMed:15057822).Curated1
Sequence conflicti456G → E in AAA41301 (PubMed:15057822).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039562216 – 276Missing in isoform 2. CuratedAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04760 mRNA Translation: AAA41301.1
RefSeqiNP_001094107.1, NM_001100637.1
UniGeneiRn.6763

Genome annotation databases

GeneIDi81002
KEGGirno:81002
UCSCiRGD:621587 rat [P36407-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTRI23_RAT
AccessioniPrimary (citable) accession number: P36407
Secondary accession number(s): D3ZT39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: August 10, 2010
Last modified: March 28, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome