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Protein

ADP-ribosylation factor-like protein 3

Gene

ARL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31MagnesiumBy similarity1
Metal bindingi48MagnesiumBy similarity1
Binding sitei48GTPBy similarity1
Binding sitei70GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4
Nucleotide bindingi159 – 161GTPBy similarity3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: Ensembl
  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB

GO - Biological processi

  • cilium morphogenesis Source: UniProtKB
  • cytokinesis Source: UniProtKB
  • intraciliary transport Source: Ensembl
  • kidney development Source: UniProtKB
  • photoreceptor cell development Source: UniProtKB
  • post-Golgi vesicle-mediated transport Source: MGI
  • small GTPase mediated signal transduction Source: UniProtKB
  • smoothened signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138175-MONOMER.
ReactomeiR-HSA-5624138. Trafficking of myristoylated proteins to the cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 3
Gene namesi
Name:ARL3
Synonyms:ARFL3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:694. ARL3.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cilium Source: UniProtKB
  • cytoplasmic microtubule Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • midbody Source: UniProtKB
  • nucleus Source: UniProtKB
  • photoreceptor connecting cilium Source: UniProtKB
  • spindle microtubule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31T → N: Enhances the interaction with RP2. 2 Publications1
Mutagenesisi71Q → L: Enhances the interaction with RP2. Does not induce a mitotic arrest resulting from the loss of the microtubule-based mitotic spindle. Induces release of myristoylated proteins from UNC119. Interacts with ARL2BP, GOLGA4, PDE6D and UNC119. 4 Publications1

Organism-specific databases

DisGeNETi403.
OpenTargetsiENSG00000138175.
PharmGKBiPA24987.

Polymorphism and mutation databases

BioMutaiARL3.
DMDMi543851.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074562 – 182ADP-ribosylation factor-like protein 3Add BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei5PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP36405.
MaxQBiP36405.
PaxDbiP36405.
PeptideAtlasiP36405.
PRIDEiP36405.

2D gel databases

REPRODUCTION-2DPAGEIPI00003327.
UCD-2DPAGEP36405.

PTM databases

iPTMnetiP36405.
PhosphoSitePlusiP36405.

Expressioni

Tissue specificityi

Expressed in the retina. Strongly expressed in connecting cilium, the myoid region of the inner segments (IS) and in cone photoreceptors (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000138175.
CleanExiHS_ARL3.
GenevisibleiP36405. HS.

Organism-specific databases

HPAiHPA036292.
HPA063596.

Interactioni

Subunit structurei

Interacts with RP2. The GTP-bound form interacts with PDE6D. Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B). Interacts with SYS1. The GTP-bound form interacts with ARL2BP and PDE6D. Interacts with RP2; interaction is direct and stimulated with the activated GTP-bound form of ARL3. Microtubule-associated protein. Does not interact with TBCC. May interact with GOLGA4.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PDE6DO439244EBI-712710,EBI-712685
UNC119Q134329EBI-712710,EBI-711260

GO - Molecular functioni

  • microtubule binding Source: UniProtKB

Protein-protein interaction databases

BioGridi106896. 9 interactors.
DIPiDIP-47537N.
IntActiP36405. 17 interactors.
MINTiMINT-1368863.
STRINGi9606.ENSP00000260746.

Structurei

3D structure databases

ProteinModelPortaliP36405.
SMRiP36405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0074. Eukaryota.
ENOG410Y825. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP36405.
KOiK07944.
OMAiIQEGMNW.
OrthoDBiEOG091G0O6V.
PhylomeDBiP36405.
TreeFamiTF105463.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLSILRKL KSAPDQEVRI LLLGLDNAGK TTLLKQLASE DISHITPTQG
60 70 80 90 100
FNIKSVQSQG FKLNVWDIGG QRKIRPYWKN YFENTDILIY VIDSADRKRF
110 120 130 140 150
EETGQELAEL LEEEKLSCVP VLIFANKQDL LTAAPASEIA EGLNLHTIRD
160 170 180
RVWQIQSCSA LTGEGVQDGM NWVCKNVNAK KK
Length:182
Mass (Da):20,456
Last modified:February 1, 1995 - v2
Checksum:iE5C1ACAD0BD55537
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01486934L → M.1 PublicationCorresponds to variant rs1141895dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07151 mRNA. Translation: AAA21654.1.
AF493889 mRNA. Translation: AAM12603.1.
AK312525 mRNA. Translation: BAG35424.1.
CR407637 mRNA. Translation: CAG28565.1.
AL391121 Genomic DNA. Translation: CAI40862.1.
BC009841 mRNA. Translation: AAH09841.1.
CCDSiCCDS7538.1.
PIRiA54869.
RefSeqiNP_004302.1. NM_004311.3.
XP_016871749.1. XM_017016260.1.
UniGeneiHs.182215.

Genome annotation databases

EnsembliENST00000260746; ENSP00000260746; ENSG00000138175.
GeneIDi403.
KEGGihsa:403.
UCSCiuc001kwa.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07151 mRNA. Translation: AAA21654.1.
AF493889 mRNA. Translation: AAM12603.1.
AK312525 mRNA. Translation: BAG35424.1.
CR407637 mRNA. Translation: CAG28565.1.
AL391121 Genomic DNA. Translation: CAI40862.1.
BC009841 mRNA. Translation: AAH09841.1.
CCDSiCCDS7538.1.
PIRiA54869.
RefSeqiNP_004302.1. NM_004311.3.
XP_016871749.1. XM_017016260.1.
UniGeneiHs.182215.

3D structure databases

ProteinModelPortaliP36405.
SMRiP36405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106896. 9 interactors.
DIPiDIP-47537N.
IntActiP36405. 17 interactors.
MINTiMINT-1368863.
STRINGi9606.ENSP00000260746.

PTM databases

iPTMnetiP36405.
PhosphoSitePlusiP36405.

Polymorphism and mutation databases

BioMutaiARL3.
DMDMi543851.

2D gel databases

REPRODUCTION-2DPAGEIPI00003327.
UCD-2DPAGEP36405.

Proteomic databases

EPDiP36405.
MaxQBiP36405.
PaxDbiP36405.
PeptideAtlasiP36405.
PRIDEiP36405.

Protocols and materials databases

DNASUi403.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260746; ENSP00000260746; ENSG00000138175.
GeneIDi403.
KEGGihsa:403.
UCSCiuc001kwa.4. human.

Organism-specific databases

CTDi403.
DisGeNETi403.
GeneCardsiARL3.
HGNCiHGNC:694. ARL3.
HPAiHPA036292.
HPA063596.
MIMi604695. gene.
neXtProtiNX_P36405.
OpenTargetsiENSG00000138175.
PharmGKBiPA24987.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0074. Eukaryota.
ENOG410Y825. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP36405.
KOiK07944.
OMAiIQEGMNW.
OrthoDBiEOG091G0O6V.
PhylomeDBiP36405.
TreeFamiTF105463.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138175-MONOMER.
ReactomeiR-HSA-5624138. Trafficking of myristoylated proteins to the cilium.

Miscellaneous databases

GeneWikiiARL3.
GenomeRNAii403.
PROiP36405.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138175.
CleanExiHS_ARL3.
GenevisibleiP36405. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARL3_HUMAN
AccessioniPrimary (citable) accession number: P36405
Secondary accession number(s): B2R6C7, Q53X83, Q5JSM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 168 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.