Reviewed, UniProtKB/Swiss-Prot P36405 (ARL3_HUMAN)

Names and origin

Protein names

Recommended name:
    ADP-ribosylation factor-like protein 3

Gene names

Name:	ARL3
Synonyms:	ARFL3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for normal cytokinesis. Does not act as an allosteric activator of the cholera toxin catalytic subunit. Ref.12
Subunit structure	Interacts with RP2, SYS1 and SCOC. Ref.8 Ref.9 Ref.10 Ref.11
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Spindle. Nucleus. Note: Present on the mitotic spindle. Ref.12 Ref.10
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Golgi apparatus Membrane Nucleus
Coding sequence diversity	Polymorphism
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
PTM	Lipoprotein Myristate
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Ref.1 Traceable author statement. Source: ProtInc magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 182	181	ADP-ribosylation factor-like protein 3		PRO_0000207456
Regions
	Nucleotide binding	24 – 31	8	GTP By similarity
	Nucleotide binding	67 – 71	5	GTP By similarity
	Nucleotide binding	126 – 129	4	GTP By similarity
	Nucleotide binding	159 – 161	3	GTP By similarity
Sites
	Metal binding	31	1	Magnesium By similarity
	Metal binding	48	1	Magnesium By similarity
	Binding site	48	1	GTP By similarity
	Binding site	70	1	GTP; via amide nitrogen By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential
Natural variations
	Natural variant	34	1	L → M: dbSNP rs1141895. Ref.4		VAR_014869

Sequences

	Sequence		Length	Mass (Da)
	P36405-1 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: E5C1ACAD0BD55537	FASTA	182	20,456
	10 20 30 40 50 60 MGLLSILRKL KSAPDQEVRI LLLGLDNAGK TTLLKQLASE DISHITPTQG FNIKSVQSQG 70 80 90 100 110 120 FKLNVWDIGG QRKIRPYWKN YFENTDILIY VIDSADRKRF EETGQELAEL LEEEKLSCVP 130 140 150 160 170 180 VLIFANKQDL LTAAPASEIA EGLNLHTIRD RVWQIQSCSA LTGEGVQDGM NWVCKNVNAK KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ADP-ribosylation factor (ARF)-like 3, a new member of the ARF family of GTP-binding proteins cloned from human and rat tissues." Cavenagh M.A., Breiner M., Schurmann A., Rosenwald A.G., Terui T., Zhang C.-J., Randoazzo P.A., Adams M., Joost H.-G., Kahn R.A. J. Biol. Chem. 269:18937-18942(1994) [PubMed: 8034651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-34.
[5]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[7]	Lubec G., Vishwanath V. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 80-97 AND 128-149, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[8]	"ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteins." Van Valkenburgh H., Shern J.F., Sharer J.D., Zhu X., Kahn R.A. J. Biol. Chem. 276:22826-22837(2001) [PubMed: 11303027] [Abstract] Cited for: INTERACTION WITH SCOC.
[9]	"Functional overlap between retinitis pigmentosa 2 protein and the tubulin-specific chaperone cofactor C." Bartolini F., Bhamidipati A., Thomas S., Schwahn U., Lewis S.A., Cowan N.J. J. Biol. Chem. 277:14629-14634(2002) [PubMed: 11847227] [Abstract] Cited for: INTERACTION WITH RP2.
[10]	"Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p." Behnia R., Panic B., Whyte J.R.C., Munro S. Nat. Cell Biol. 6:405-413(2004) [PubMed: 15077113] [Abstract] Cited for: INTERACTIONN WITH SYS1, SUBCELLULAR LOCATION.
[11]	"Crystal structure of the human retinitis pigmentosa 2 protein and its interaction with Arl3." Kuehnel K., Veltel S., Schlichting I., Wittinghofer A. Structure 14:367-378(2006) [PubMed: 16472755] [Abstract] Cited for: INTERACTION WITH RP2.
[12]	"Specificity of Arl2/Arl3 signaling is mediated by a ternary Arl3-effector-GAP complex." Veltel S., Kravchenko A., Ismail S., Wittinghofer A. FEBS Lett. 582:2501-2507(2008) [PubMed: 18588884] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U07151 mRNA. Translation: AAA21654.1. AF493889 mRNA. Translation: AAM12603.1. AK312525 mRNA. Translation: BAG35424.1. CR407637 mRNA. Translation: CAG28565.1. AL391121 Genomic DNA. Translation: CAI40862.1. BC009841 mRNA. Translation: AAH09841.1.
IPI	IPI00003327.
PIR	A54869.
RefSeq	NP_004302.1.
UniGene	Hs.182215
3D structure databases
HSSP	HSSP built from PDB template 1FZQ based on UniProtKB Q9WUL7.
SMR	P36405. Positions 2-177.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36405. 6 interactions.
2-D gel databases
REPRODUCTION-2DPAGE	IPI00003327.
Proteomic databases
PeptideAtlas	P36405.
PRIDE	P36405.
Genome annotation databases
Ensembl	ENSG00000138175. Homo sapiens. [Contig view]
GeneID	403.
KEGG	hsa:403.
Organism-specific databases
GeneCards	GC10M104423.
H-InvDB	HIX0009165.
HGNC	HGNC:694. ARL3.
MIM	604695. gene.
PharmGKB	PA24987.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P36405.
HOVERGEN	P36405.
OMA	P36405. PTAGFNI.
Gene expression databases
ArrayExpress	P36405.
Bgee	P36405.
CleanEx	HS_ARL3.
GermOnline	ENSG00000138175. Homo sapiens.
Family and domain databases
InterPro	IPR006688. ARF. IPR006689. ARF/SAR. IPR005225. Small_GTP_bd. [Graphical view]
PANTHER	PTHR11711. ARF/SAR. 1 hit.
Pfam	PF00025. Arf. 1 hit. [Graphical view]
PRINTS	PR00328. SAR1GTPBP.
SMART	SM00177. ARF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51417. ARF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	1689.
SOURCE	Search...

Entry information

Entry name

ARL3_HUMAN

Accession

Primary (citable) accession number: P36405
Secondary accession number(s): B2R6C7, Q53X83, Q5JSM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)