Reviewed, UniProtKB/Swiss-Prot P36404 (ARL2_HUMAN)

Names and origin

Protein names

Recommended name:
    ADP-ribosylation factor-like protein 2

Gene names

Name:

ARL2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Component of a regulated secretory pathway involved in Ca2+-dependent release of acetylcholine. Regulates formation of new microtubules. Required for normal progress through the cell cycle. Ref.7
Subunit structure	Interacts with SCOC and ELMOD2. The GTP-bound form interacts with ARL2BP. Ref.5 Ref.6 Ref.8
Subcellular location	Centrosome. Ref.7
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Biological process	Cell cycle
Coding sequence diversity	Polymorphism
Ligand	GTP-binding Nucleotide-binding
PTM	Isopeptide bond Lipoprotein Myristate Ubl conjugation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro tubulin complex assembly Traceable author statement. Source: ProtInc
Cellular component	centrosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase inhibitor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 184	183	ADP-ribosylation factor-like protein 2		PRO_0000207453
Regions
	Nucleotide binding	23 – 30	8	GTP By similarity
	Nucleotide binding	66 – 70	5	GTP By similarity
	Nucleotide binding	125 – 128	4	GTP By similarity
Sites
	Binding site	68	1	GTP; via amide nitrogen By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential
	Cross-link	71		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.9
Natural variations
	Natural variant	141	1	V → A: dbSNP rs664226. Ref.1 Ref.3		VAR_028056
Experimental info
	Mutagenesis	70	1	Q → L: Cell cycle arrest, reduced ability to form microtubules, and centrosome fragmentation. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P36404-1 [UniParc]. Last modified October 17, 2006. Version 4. Checksum: 0823F005719C17F9	FASTA	184	20,878
	10 20 30 40 50 60 MGLLTILKKM KQKERELRLL MLGLDNAGKT TILKKFNGED IDTISPTLGF NIKTLEHRGF 70 80 90 100 110 120 KLNIWDVGGQ KSLRSYWRNY FESTDGLIWV VDSADRQRMQ DCQRELQSLL VEERLAGATL 130 140 150 160 170 180 LIFANKQDLP GALSSNAIRE VLELDSIRSH HWCIQGCSAV TGENLLPGID WLLDDISSRI FTAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Selective amplification of additional members of the ADP-ribosylation factor (ARF) family: cloning of additional human and Drosophila ARF-like genes." Clark J., Moore L., Krasinskas A., Way J., Battey J.F., Tamkun J.W., Kahn R.A. Proc. Natl. Acad. Sci. U.S.A. 90:8952-8956(1993) [PubMed: 8415637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-141.
[2]	Kahn R.A. Submitted (NOV-1997) to UniProtKB Cited for: SEQUENCE REVISION TO 11.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-141. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[5]	"The ARF-like 2 (ARL2)-binding protein, BART. Purification, cloning, and initial characterization." Sharer J.D., Kahn R.A. J. Biol. Chem. 274:27553-27561(1999) [PubMed: 10488091] [Abstract] Cited for: INTERACTION WITH ARL2BP.
[6]	"ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteins." Van Valkenburgh H., Shern J.F., Sharer J.D., Zhu X., Kahn R.A. J. Biol. Chem. 276:22826-22837(2001) [PubMed: 11303027] [Abstract] Cited for: INTERACTION WITH SCOC.
[7]	"Arl2 and Arl3 regulate different microtubule-dependent processes." Zhou C., Cunningham L., Marcus A.I., Li Y., Kahn R.A. Mol. Biol. Cell 17:2476-2487(2006) [PubMed: 16525022] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLN-70, SUBCELLULAR LOCATION.
[8]	"ELMOD2 is an Arl2 GTPase-activating protein that also acts on Arfs." Bowzard J.B., Cheng D., Peng J., Kahn R.A. J. Biol. Chem. 282:17568-17580(2007) [PubMed: 17452337] [Abstract] Cited for: INTERACTION WITH ELMOD2.
[9]	"Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry." Meierhofer D., Wang X., Huang L., Kaiser P. J. Proteome Res. 7:4566-4576(2008) [PubMed: 18781797] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-71, MASS SPECTROMETRY.
[10]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L13687 mRNA. Translation: AAC37606.1.
AF493888 mRNA. Translation: AAM12602.1.
BC002530 mRNA. Translation: AAH02530.1.

IPI

IPI00003326.

PIR

A48259.

RefSeq

NP_001658.2.

UniGene

Hs.502836

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DOE	X-ray	2.25	A	1-184	[»]
3DOF	X-ray	3.30	A	1-184	[»]

SMR

P36404. Positions 1-179.

ModBase

Search...

Protein-protein interaction databases

IntAct

P36404. 1 interaction.

PTM databases

PhosphoSite

P36404.

Proteomic databases

PRIDE

P36404.

Genome annotation databases

Ensembl

ENSG00000213465. Homo sapiens. [Contig view]

GeneID

402.

KEGG

hsa:402.

Organism-specific databases

GeneCards

GC11P064539.

H-InvDB

HIX0079275.
HIX0079396.

HGNC

HGNC:693. ARL2.

MIM

601175. gene.

PharmGKB

PA24986.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P36404.

OMA

P36404. TDGLVWV.

Gene expression databases

ArrayExpress

P36404.

Bgee

P36404.

CleanEx

HS_ARL2.

GermOnline

ENSG00000110025. Homo sapiens.

Family and domain databases

InterPro

IPR006688. ARF.
IPR006689. ARF/SAR.
IPR005225. Small_GTP_bd.
[Graphical view]

PANTHER

PTHR11711. ARF/SAR. 1 hit.

Pfam

PF00025. Arf. 1 hit.
[Graphical view]

PRINTS

PR00328. SAR1GTPBP.

SMART

SM00177. ARF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51417. ARF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

1685.

SOURCE

Search...

Entry information

Entry name

ARL2_HUMAN

Accession

Primary (citable) accession number: P36404
Secondary accession number(s): Q9BUK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 86 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)